Difference In Turnaround Time Research Articles

Precision Pathology: Unveiling the role of Interval Sectioning Protocol for Optimal Diagnosis in Pancreatic, Ampullary, and Bile Duct Biopsies

Abstract Introduction/Objective Endoscopic techniques offer advanced diagnostics but encounter challenges in obtaining limited pancreaticobiliary biopsies, including ampullary (AMP), EUS-guided pancreatic (PAN), and spy bite biliary (BD) biopsies. Interval sectioning optimizes immunohistochemical stain utility and tissue conservation. This study evaluates interval sectioning protocols’ impact on diagnostic precision and tissue preservation at our institution. Methods/Case Report This analysis examines cases over four months before and after a protocol change, focusing on AMP, BD, and PAN biopsies. Previously, AMP and BD biopsies used three H&E-stained slides, while PAN required two H&E-stained and five unstained slides (USS). The new protocol adopts seven slides with H&E staining on levels 1, 4, and 7, emphasizing consistency. Parameters assessed include diagnosis, turnaround time (TAT), and USS utilization. A 30% USS utilization cutoff justifies upfront interval sectioning. Results (if a Case Study enter NA) A total of 77 cases from the old protocol and 142 from the new protocol were reviewed. No significant difference in TAT between old and new protocols was found (p=0.340). However, PAN TAT notably improved (4848 min vs. 2932 min). Usage rates varied by site: 22.2% (AMP), 33.3% (BD), and 34.4% (PAN). Conclusion USS usage was lowest for AMP cases (22.2%), below the 30% threshold for upfront interval sectioning justification. Eliminating interval sectioning for AMP would save 116 USS in the new protocol. BD and PAN cases warrant upfront interval sectioning, with USS usage rates of 31.5% and 33.7%, respectively. While PAN cases show no cost difference, the new protocol improves specimen visualization, correlates immunohistochemical stains better, and significantly enhances turnaround time.

Abbreviated MRI in the evaluation of dizziness: report turnaround times and impact on length of stay compared to CT, CTA, and conventional MRI.

Neuroimaging is often used in the emergency department (ED) to evaluate for posterior circulation strokes in patients with dizziness, commonly with CT/CTA due to speed and availability. Although MRI offers more sensitive evaluation, it is less commonly used, in part due to slower turnaround times. We assess the potential for abbreviated MRI to improve reporting times and impact on length of stay (LOS) compared to conventional MRI (as well as CT/CTA) in the evaluation of acute dizziness. We performed a retrospective analysis of length of stay via LASSO regression for patients presenting to the ED with dizziness and discharged directly from the ED over 4 years (1/1/2018-12/31/2021), controlling for numerous patient-level and logistical factors. We additionally assessed turnaround time between order and final report for various imaging modalities. 14,204 patients were included in our analysis. Turnaround time for abbreviated MRI was significantly lower than for conventional MRI (4.40h vs. 6.14h, p < 0.001) with decreased impact on LOS (0.58h vs. 2.02h). Abbreviated MRI studies had longer turnaround time (4.40h vs. 1.41h, p < 0.001) and was associated with greater impact on ED LOS than non-contrast CT head (0.58h vs. 0.00h), however there was no significant difference in turnaround time compared to CTA head and neck (4.40h vs. 3.86h, p = 0.06) with similar effect on LOS (0.58h vs. 0.53h). Ordering both CTA and conventional MRI was associated with a greater-than-linear increase in LOS (additional 0.37h); the same trend was not seen combining CTA and abbreviated MRI (additional 0.00h). In the acute settings where MRI is available, abbreviated MRI protocols may improve turnaround times and LOS compared to conventional MRI protocols. Since recent guidelines recommend MRI over CT in the evaluation of dizziness, implementation of abbreviated MRI protocols has the potential to facilitate rapid access to preferred imaging, while minimizing impact on ED workflows.

The Impact of Laboratory Automation on the Time to Urine Microbiological Results: A Five-Year Retrospective Study.

Total laboratory automation (TLA) is a valuable component of microbiology laboratories and a growing number of publications suggest the potential impact of automation in terms of analysis standardization, streaking quality, and the turnaround time (TAT). The aim of this project was to perform a detailed investigation of the impact of TLA on the workflow of commonly treated specimens such as urine. This is a retrospective observational study comparing two time periods (pre TLA versus post TLA) for urine specimen culture processing. A total of 35,864 urine specimens were plated during the pre-TLA period and 47,283 were plated during the post-TLA period. The median time from streaking to identification decreased from 22.3 h pre TLA to 21.4 h post TLA (p < 0.001), and the median time from streaking to final validation of the report decreased from 24.3 h pre TLA to 23 h post TLA (p < 0.001). Further analysis revealed that the observed differences in TAT were mainly driven by the contaminated and positive samples. Our findings demonstrate that TLA has the potential to decrease turnaround times of samples in a laboratory. Nevertheless, changes in laboratory workflow (such as extended opening hours for plate reading and antibiotic susceptibility testing or decreased incubation times) might further maximize the efficiency of TLA and optimize TATs.

Comparison of Aerobic Bacterial Culture Among Four Veterinary Microbiology Laboratories from Dogs with Superficial Pyoderma.

Bacterial culture and susceptibility are widely used in veterinary medicine to determine the specific bacteria causing infection as well as aid in appropriate antimicrobial selection. Previous studies have shown variable results with culture and susceptibility depending on the laboratory and methodology used. Samples from dogs with superficial pyoderma were obtained to make a homogeneous solution of bacteria. Sample acquisition from this solution was randomized and submitted to four veterinary laboratories for microbial identification and sensitivity. There was fair agreement among the laboratories in identification of a Staphylococcus spp. as well as fair agreement among the laboratories on the same Staphylococcus sp. Very good agreement was noted on identification of methicillin-resistant Staphylococcus spp. Additionally, good to very good agreement was noted on all antimicrobials that were tested across all four laboratories. A difference in turnaround time for sample processing was observed between the laboratories in the present study. Overall, there was mild variability among the laboratory results in this study.

Perbandingan Turnaround Time Antara Verifikasi Manual dan Verifikasi Otomatis terhadap Hasil Pemeriksaan Profil Lipid di Laboratorium Klinik Pramita Cabang Pajajaran Kota Bandung

Turnaround Time (TAT) for laboratory services is an indicator of the quality of laboratory services to meet customer satisfaction and as a benchmark for laboratory performance. Laboratory results are very important to be delivered in the shortest possible time for the rapid diagnosis and management of patients. One of the factors that causes the length of TAT in the reporting process at the analytical stage is the verification process of inspection results which is still carried out manually by ATLM personnel. Implementing automatic verification of examination results is one way to overcome the long waiting time (TAT) for laboratory services. The aim of this research is to determinate the TAT of manual verification and automatic verification of lipid profile examination results, and testing the difference in TAT between manual verification and automatic verification of lipid profile examination results at the analytical stage at the Pramita clinical laboratory, Pajajaran branch in Bandung city. The method used in this research is to use descriptive comparative method with a quantitative approach. Based on the research that has been done, the average ± standard deviation TAT for manual verification of the results of lipid profile examination was 112.44 ± 46.02 minutes, while the automatic verification TAT was 68.03 ± 28.07 minutes. The results of the independent sample t-test between TAT manual verification and automatic verification of the lipid profile examination results with a significance value of Sig.(2-tailed) 0.000 &lt;0.05. The conclusion of this research is that TAT verification of lipid profile examination results at the analytical stage decreased after automatic verification was implemented. There is a significant difference in Turnaround Time between manual verification and automatic verification of the results of lipid profile examinations at the analytical stage at the Pramita Clinical Laboratory, Pajajaran branch in Bandung city.&#x0D; &#x0D;

A side-by-side comparison of the new VITEK MS PRIME and the MALDI Biotyper sirius in the clinical microbiology laboratory

PurposeThis study aims to evaluate the performance of two latest generation matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) systems in routine laboratory settings, focusing on turnaround time (TAT), time to results (TTR), hands-on time, and identification rate.MethodsWe conducted a time and motion study on three workflow scenarios to simulate different laboratory settings. Overall, 618 bacterial isolates from a tertiary hospital’s laboratory were processed using the VITEK MS PRIME (bioMérieux) and the MALDI Biotyper sirius (Bruker Daltonics) and their corresponding databases VITEK IVD Database 3.2 and MBT reference library 12.ResultsThe target preparation process showed no significant difference in TAT, but the Biotyper workflow had a shorter hands-on time by 3 to 6 min. In the measurement process, TTR was three to five times shorter for the Biotyper sirius while hands-on time was significantly shorter for VITEK MS PRIME (approximately 1.5 min per target). The identification rate without retesting was 97.9% for VITEK MS PRIME and 98.9% for Biotyper sirius. Both systems achieved 100% agreement at genus and 96.2% at species level.ConclusionBoth systems exhibited excellent identification rates for routine bacterial isolates. Due to its high speed, the Biotyper sirius is suited for laboratories with high sample throughput and a workflow designed for processing larger batches. The VITEK MS PRIME, with its “load and go” system accommodating up to 16 targets, reduces hands-on time, making it a reasonable choice for laboratories with fewer identifications overall but a higher number of targets and a workflow designed for parallel processing on different workstations.

Penerapan Metode Weight Product (WP) untuk Rekomendasi Pemilihan Jasa Paket Laundry Terbaik

Laundy is an activity of washing clothes or the like at the same time and place in one place. The author takes samples to select package services from one of the shops or laundry businesses, namely dry regular, dry medium and dry express which customers can choose from with differences in turnaround time, weight and price, and can help laundry shop owners find out the type of packages that are often chosen by customers and can make it easier for shop owners to find out the results of sales transactions and reports. To understand this, we need a recommendation system that can help customers and laundry shop owners according to what the author wants. Here the author uses the Product Weight method to find alternative values ​​or the best results using several calculation formulas and gets a result of 0.335 on R2, namely Dry Medium with price criteria, completion time and weight

Resident pre-dictation of ED case delay signoff of those reports, but overall, residents improve report turnaround time.

In academic radiology departments, attendings and resident are increasingly working together overnight for Emergency Department (ED) radiology coverage. The purpose of this study was to quantify reporting turnaround time for overnight cases read by residents and for overnight shifts with residents on duty. A retrospective study was performed at a hospital system where one overnight attending covers two hospitals with a 2nd/3rd year overnight resident, and a second overnight attending covers two other hospitals 80% of the time independently and 20% of the time with a fourth-year resident. In the first analysis, the median difference in turnaround time, from the time when the case was completed by the technologist to the time the attending finalized it, between cases read independently by attendings and cases pre-dictated by residents was calculated. In the second analysis, the median difference in turnaround time for all cases performed at the second two hospitals was compared on nights when an attending was on duty alone versus nights when a fourth-year resident was also on duty, regardless of if the resident had pre-dictated the case. For computed tomography (CT), radiographs (XR), and ultrasound (US), there was a significant delay in turnaround time for cases pre-dictated by residents compared to cases read independently by attendings, ranging between 11 and 49 min depending on resident seniority and modality (p ≤ 0.001). For all cases on nights with a 4th year resident working, overall median report turnaround time decreased by 7 min (p < 0.001). Resident pre-dictation causes delay in the finalization of individual CT, US, and XR reports; however, overall, working with residents results in a significant decrease in report turnaround time supporting the belief that overnight resident education does not delay patient care.

Time to publish? Turnaround times, acceptance rates, and impact factors of journals in fisheries science.

Selecting a target journal is a universal decision faced by authors of scientific papers. Components of the decision, including expected turnaround time, journal acceptance rate, and journal impact factor, vary in terms of accessibility. In this study, I collated recent turnaround times and impact factors for 82 journals that publish papers in the field of fisheries sciences. In addition, I gathered acceptance rates for the same journals when possible. Findings indicated clear among-journal differences in turnaround time, with median times-to-publication ranging from 79 to 323 days. There was no clear correlation between turnaround time and acceptance rate nor between turnaround time and impact factor; however, acceptance rate and impact factor were negatively correlated. I found no field-wide differences in turnaround time since the beginning of the COVID-19 pandemic, though some individual journals took significantly longer or significantly shorter to publish during the pandemic. Depending on their priorities, authors choosing a target journal should use the results of this study as guidance toward a more informed decision.

A Tertiary Academic Medical Center Blood Bank's Experience With Four-Factor Prothrombin Complex Concentrate.

The role of transfusion medicine consultative services in prospectively auditing (PA) orders for four-factor prothrombin complex concentrate (4F-PCC) was evaluated at an academic medical center. Data from 4 years of 4F-PCC orders were obtained from the laboratory information system, and electronic health records of patients receiving concentrate were reviewed. 4F-PCC was ordered for 427 patients with warfarin-, apixaban-, or rivaroxaban-associated hemorrhage. Turnaround time (TAT) to prepare 4F-PCC was longer when PA-recommended dose adjustments were needed (85 vs 66 minutes, P = .03). There was no difference in TAT between patients who died and those who were ultimately discharged (60 vs 70, P = .22). TAT was shortest for orders originating in the emergency department (ED) compared with other locations (64 vs 85, P < .001), and ED TAT was not associated with patient outcomes in ED patients. PA and dose adjustments reduced amounts of concentrate issued by 27 IU per dose (P = .01). Median international normalized ratio less than 1.3 after 4F-PCC transfusion was achieved for all anticoagulants after dose adjustments. PA did not affect order cancellation or product wastage rates. PA can ensure 4F-PCC is dosed appropriately without affecting patient outcomes.

Assessment of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF- MS) for Accurate Bacterial Identification in Clinical Labs

Abstract Introduction/Objective On behalf of the diagnostic Medical Laboratory rapid, and accurate identification of bacteria with their one-to-one anti-microbial susceptibility outlines is of ultimate importance for the management of infected patients. Contemporary microbial identification methods employed in routine clinical diagnostic laboratories relies on the use of conventional phenotypic methods. Phenotypic methods are time consuming with minimum turn-around times of at least 24 hrs and in many occurrences of 48hrs. With the intention of accelerate laboratory processes the MALDI-TOF MS was familiarized. MALDI-TOF MS is established on proteomic profiling and permits for rapid identification of bacteria. This technology has not been widely used in Egypt, but has been regularly used in Europe for the past few years. Methods Two hundred forty three positive non duplicate blood cultures were accrued over a period of six months. Experimental aliquots were taken from excess sample material that was collected as part of routine clinical care. 105 were positive for Gram negative bacilli, 123 were positive for Gram positive cocci, 3 positive for Gram positive bacilli, and 7 were positive for yeast. MALDI-TOF identification was compared to conventional identification. Conventional identification consisted of a combination of MALDI-TOF identification of a subcultures colony by direct smear, biochemical reactions, Vitek 2, and molecular identification. Results Ninety seven of the one hundred and five blood cultures positive for Gram negative bacilli were monomicrobial. The majority of these were identified as Escherichia coli by conventional methods, followed by Klebsiella pneumoniae and Pseudomonas aeruginosa. Eighty-four of these monomicrobial cultures were identified by MALDI-TOF to the species level. Eighty-one of the eighty-four were concordant with the conventional identification (96.4%). Conclusion The MALDI-TOF proved to be useful for the rapid and reliable identification of g-ve bacteria from the clinical specimens. The difference in turnaround time for bacterial identification was significant between MALDI-TOF MS and VITEK 2 with minimal preparation for the blood cultures.

Interpretation of Discordant Rifampicin Susceptibility Test Results Obtained Using GeneXpert vs Phenotypic Drug Susceptibility Testing.

BackgroundThe 3-month difference in turnaround time between Xpert and conventional phenotypic drug susceptibility testing (pDST) causes patient treatment challenges when pDST rifampin (RIF) susceptibility results and earlier Xpert results disagree, resulting in unnecessary tuberculosis (TB) patient exposure to toxic second-line drugs. Here, the prevalence of discordant RIF susceptibility test results, specifically Xpert (resistant) vs pDST (susceptible) results, was determined.MethodsTuberculosis patients enrolled between January 2015 and June 2018 at Beijing Chest Hospital who consecutively tested positive for RIF resistance using Xpert then negative using pDST were studied. DNA sequences and minimal inhibitory concentration (MIC) results provided insights for understanding discordant results.ResultsOf 26 826 patients with suggestive TB symptoms undergoing Xpert MTB/RIF testing, 728 diagnosed as RIF-resistant were evaluated. Of these, 118 (16.2%) exhibiting Xpert RIF resistance and phenotypic RIF susceptibility yielded 104 successfully subcultured isolates; of these, 86 (82.7%) harbored rpoB gene RIF resistance–determining region mutations and 18 (17.3%) did not. The Leu511Pro (25.0%) and Leu533Pro (17.3%) mutations were most frequently associated with discordant RIF susceptibility test results. Of the 86 isolates with rpoB mutations, 42 (48.8%) with MICs ≤1.0 mg/L were assigned to the RIF-susceptible group, with Leu511Pro being the most common mutation observed. Isolates with a very low bacterial load were most frequently misdiagnosed as RIF-resistant by Xpert.ConclusionsApproximately one-sixth of RIF-resistant TB isolates identified via Xpert yielded discordant pDST results due to questionable interpretation of specific “disputed” mutations. Thus, a diagnostic flowchart should be used to correctly interpret Xpert RIF resistance results to best guide patient treatment.

Abstract TP360: Triage Nursing Intervention Improves Stroke Treatment Rate and Times for Walk-In Strokes

Introduction: Our comprehensive stroke center provides community outreach and stroke education to patients, caregivers, and community members on the importance of calling 911 in the event of a stroke. However, approximately 1/3 of our center’s stroke alerts are walk-ins. With a walk-in stroke, rapid assessment is essential because the stroke response team has no information compared to information that otherwise would be provided by EMS. As such, our center developed a rapid assessment by the emergency triage nurse or technician, who can then activate a stroke alert. Methods: The change to rapid stroke assessment and stroke alert activation by triage nurses and technicians (rather than waiting for an emergency physician to assess and activate a stroke alert) was made in March 2018. Cases from one year prior to the intervention were compared to cases from the year after implementation. Differences in turnaround times (door to stroke alert activation, door to needle [DTN]) were calculated. Results: In the period before implementation, there were 1200 stroke alerts, of which 420 arrived via triage (35%). Median door to stroke alert was 0 min. Of those who arrived through triage, 8 received IV alteplase (8/420=2%). For those patients, median DTN was 39 min. In contrast, after implementation, there were 1401 stroke alerts, of which 342 arrived via triage (24%). Median door to stroke alert was 2 min. Of those, 15 received IV alteplase (15/342=4%), with a median DTN of 32 min. Discussion: A nursing driven initiative at Emergency Department triage was effective at improving stroke treatment rate and decreasing DTN for IV alteplase for walk-in stroke patients.

Neurological Dashboards and Consultation Turnaround Time at an Academic Medical Center.

Neurologists perform a significant amount of consultative work. Aggregative electronic health record (EHR) dashboards may help to reduce consultation turnaround time (TAT) which may reflect time spent interfacing with the EHR. This study was aimed to measure the difference in TAT before and after the implementation of a neurological dashboard. We retrospectively studied a neurological dashboard in a read-only, web-based, clinical data review platform at an academic medical center that was separate from our institutional EHR. Using our EHR, we identified all distinct initial neurological consultations at our institution that were completed in the 5 months before, 5 months after, and 12 months after the dashboard go-live in December 2017. Using log data, we determined total dashboard users, unique page hits, patient-chart accesses, and user departments at 5 months after go-live. We calculated TAT as the difference in time between the placement of the consultation order and completion of the consultation note in the EHR. By April 30th in 2018, we identified 269 unique users, 684 dashboard page hits (median hits/user 1.0, interquartile range [IQR] = 1.0), and 510 unique patient-chart accesses. In 5 months before the go-live, 1,434 neurology consultations were completed with a median TAT of 2.0 hours (IQR = 2.5) which was significantly longer than during 5 months after the go-live, with 1,672 neurology consultations completed with a median TAT of 1.8 hours (IQR = 2.2; p = 0.001). Over the following 7 months, 2,160 consultations were completed and median TAT remained unchanged at 1.8 hours (IQR = 2.5). At a large academic institution, we found a significant decrease in inpatient consult TAT 5 and 12 months after the implementation of a neurological dashboard. Further study is necessary to investigate the cognitive and operational effects of aggregative dashboards in neurology and to optimize their use.

Blast flagging of the Sysmex XN-10 hematology analyzer with supervised cell image analysis: Impact on quality parameters.

The Sysmex XN-10 automated hematology analyzer (Sysmex Corporation) is routinely used in hematology laboratories to perform complete blood cell count with differential (CBC w/ diff). The sensitivity of this system for blast detection is unclear, since many prior studies evaluating the blast flagging capabilities of Sysmex XN series used the white precursor cell (WPC) channel, which is not cleared for use in the United States. We assessed the blast flagging capabilities of the Sysmex XN-10 compared with CellaVision (a cell image analyzer)-assisted visual hematology results. We evaluated the following flags: "blasts?/abnormal lymph?" and "immature granulocytes present" and compared differences in turnaround time between methods. We collected data on 2239 CBC w/ diff Sysmex automated analyzer differential and CellaVision-assisted visual differential from the inpatient hematology-oncology population of a tertiary care medical center. Solely analyzing the first CBC/diff from each unique patient, both flags had a combined sensitivity of 100%, specificity of 50.2%, PPV of 21.7%, and NPV of 100%. The mean turnaround time for the automated differential was 19.5minutes (SD35.9minutes) compared with 66.4minutes for the CellaVision-assisted visual differential (SD68.5minutes; P<0.001; Figure 1). The Sysmex XN-10 abnormal lymphocyte/blast and immature granulocytes flags had excellent sensitivity and acceptable specificity in detecting circulating blasts with shorter turnaround time than the CellaVision-assisted visual differential. Our study suggests that automated differentials performed on Sysmex XN-10 can replace visual differentials as a first-line screening method for blast detection with improved turnaround time in hematology-oncology populations.

Use of matrix-assisted laser desorption/ionisation-time of flight mass spectrometry analyser in a diagnostic microbiology laboratory in a developing country.

BackgroundRapid and accurate identification of pathogens is of utmost importance for management of patients. Current identification relies on conventional phenotypic methods which are time consuming. Matrix-assisted laser desorption/ionisation-time of flight mass spectrometry (MALDI-TOF MS) is based on proteomic profiling and allows for rapid identification of pathogens.ObjectiveWe compared MALDI-TOF MS against two commercial systems, MicroScan Walkaway and VITEK 2 MS.MethodsOver a three-month period from July 2013 to September 2013, a total of 227 bacteria and yeasts were collected from an academic microbiology laboratory (N = 121; 87 Gram-negatives, seven Gram-positives, 27 yeasts) and other laboratories (N = 106; 35 Gram-negatives, 34 Gram-positives, 37 yeasts). Sixty-five positive blood cultures were initially processed with Bruker Sepsityper kit for direct identification.ResultsFrom the 65 blood culture bottles, four grew more than one bacterial pathogen and MALDI-TOF MS identified only one isolate. The blood cultures yielded 21 Gram-negatives, 43 Gram-positives and one Candida. There were 21 Escherirchia coli isolates which were reported by the MALDI-TOF MS as E. coli/Shigella. Of the total 292 isolates, discrepant results were found for one bacterial and three yeast isolates. Discrepant results were resolved by testing with the API system with MALDI-TOF MS showing 100% correlation.ConclusionThe MALDI-TOF MS proved to be very useful for rapid and reliable identification of bacteria and yeasts directly from blood cultures and after culture of other specimens. The difference in time to identification was significant for all isolates. However, for positive blood cultures with minimal sample preparation time there was a massive difference in turn-around time with great appreciation by clinicians.

Comparative performance study of six commercial molecular assays for rapid detection of toxigenic Clostridium difficile

ObjectivesRapid and accurate detection of Clostridium difficile in stool affects patient treatment and containment efforts. Detection of C. difficile toxin genes using nucleic acid amplification techniques (NAAT) is part of a multistep algorithm. Our objective was to directly compare the diagnostic accuracy and applicability of six commercial C. difficile NAAT. MethodsTwo hundred ten specimens were analysed in parallel by six commercial NAAT. Toxigenic culture was used as a reference method. ResultsWe analysed 98 positive and 112 negative samples. The Xpert C. difficile had 99% sensitivity (95% confidence interval (CI) 94.45–99.97), followed by Simplexa C. difficile Universal Direct 95% (95% CI 88.49–98.32), Illumigene C. difficile, and Quidel AmpliVue C. difficile, both 93% (95% CI 85.84–97.08), and BDmax Cdiff and GenomEra C. difficile, both 92% (95% CI 84.55–96.41). All assays had very high specificity (>99%). Invalid results requiring retesting were the highest in GenomEra (6.7%; 14/210) and BDmax (4.3%; 9/210), followed by AmpliVue (1.4%; 3/210) and Xpert (0.96%; 2/210). No retesting was required with Simplexa and Illumigene. The turnaround time was the shortest for the Illumigene and Xpert and the longest for BDmax, mostly due to the different reaction times of assays. Total hands-on time was comparable for all six assays. ConclusionsAll assays had high sensitivity and specificity. The differences in turnaround time, repeat testing rates and platform characteristics could help laboratories decide which assay would integrate better in their setting and to better select a molecular platform for C. difficile detection.

Cell‐free DNA testing: how to choose which laboratory to use?

Cell‐free <scp>DNA</scp> testing: how to choose which laboratory to use?

Assessment of suitability of the one step nucleic acid amplification (OSNA) assay as an intraoperative procedure for detection of metastasis in sentinel lymph nodes of breast cancer

AimWe aimed to assess the one step nucleic acid amplification (OSNA) assay as an intraoperative method in comparison with frozen sections (FS) for detection of metastasis in sentinel lymph nodes...

Communication gaps in nursing home transfers to the ED: impact on turnaround time, disposition, and diagnostic testing

IntroductionThis study aims to determine the source of communication gaps in history of present illness (HPI), medical history, and advanced directives in nursing home (NH) patients transferred to the emergency department (ED). We also attempt to determine if these gaps create differences in patient turnaround time (TAT), disposition decision, or diagnostic testing. MethodsA convenience sample of patients transferred from NHs to a level 1 community trauma center was enrolled by the physicians caring for them. The physicians assessed the adequacy and source of the history for each patient. The patient's chart was then retrospectively reviewed to determine disposition, ED TAT, and diagnostic tests ordered. ResultsOne hundred patients were enrolled. Physicians found that NH paperwork contained adequate HPI 35% of the time. Patients could provide their own HPI 28% of the time. In 32% of patients, adequate HPI could not be obtained from the patient, NH paperwork, or NH personnel. Comparing patients in whom adequate HPI was available (n = 68) to those in whom HPI was not available (n = 32), there was no difference in TAT (146 vs 173 minutes, P = .22), admissions (60% vs 66%, P = .66), or diagnostic testing (P = .89-1.0). ConclusionEmergency department physicians often do not have adequate HPI in patients transferred from NHs. The absence of adequate information does not affect patient TAT, disposition decision, or ED diagnostic testing.