Differences In Protein Metabolism Research Articles

Chronic social stress alters protein metabolism in juvenile rainbow trout, Oncorhynchus mykiss

When confined in pairs, juvenile rainbow trout (Oncorhynchus mykiss) form dominance hierarchies in which subordinate fish exhibit characteristic physiological changes including reduced growth rates and chronically elevated plasma cortisol concentrations. We hypothesized that alterations in protein metabolism contribute to the reduced growth rate of socially stressed trout, and predicted that subordinate trout would exhibit reduced rates of protein synthesis coupled with increases in protein degradation. Protein metabolism was assessed in dominant and subordinate fish after 4 days of social interaction, and in fish that were separated after 4 days of interaction for a 4 days recovery period, to determine whether effects on protein metabolism recovered when social stress was alleviated. Protein metabolism was assessed in liver and white muscle by measuring the fractional rate of protein synthesis and markers of protein degradation. In the white muscle of subordinate fish, protein synthesis was inhibited and activities of the ubiquitin-proteasome pathway (UPP) and the autophagy lysosomal system (ALS) were elevated. By contrast, the liver of subordinate fish exhibited increased rates of protein synthesis and activation of the ALS. When allowed to recover from chronic social stress for 4 days, differences in protein metabolism observed in white muscle of subordinate fish during the interaction period disappeared. In liver, protein synthesis returned to baseline levels during recovery from social stress, but markers of protein degradation did not. Collectively, these data support the hypothesis that inhibition of muscle protein synthesis coupled with increases in muscle protein breakdown contribute to the reduced growth rates of subordinate rainbow trout.

Differential protein metabolism and regeneration in hypertrophic diaphragm and atrophic gastrocnemius muscles in hibernating Daurian ground squirrels.

What is the central question of this study? The aim was to investigate whether diaphragm hypertrophy and gastrocnemius atrophy during hibernation of Daurian ground squirrels involve differential regulation of protein metabolism and regeneration. What is the main finding and its importance? We clarified the differences in protein metabolism and muscle regenerative potential in the diaphragm and gastrocnemius of hibernating ground squirrels, reflecting the different adaptability of muscles. Are differences in the regulation of protein metabolism and regeneration involved in the different phenotypic adaptation mechanisms of muscle hypertrophy and atrophy in hibernators? Two fast-type muscles (diaphragm and gastrocnemius) in summer active and hibernating Daurian ground squirrels were selected to detect changes in cross-sectional area (CSA) and protein expression indicative of protein synthesis metabolism (protein expression of P-Akt, P-mTORC1, P-S6K1 and P-4E-BP1), protein degradation metabolism (MuRF1, atrogin-1, calpain-1, calpain-2, calpastatin, desmin, troponinT, Beclin1 and LC3-II) and muscle regeneration (MyoD, myogenin and myostatin). In the hibernation group compared with the summer active group, the CSA of the diaphragm muscle increased significantly by 26.1%, whereas the CSA of the gastrocnemius muscle decreased significantly by 20.4%. Our study also indicated that increased protein synthesis, decreased protein degradation and increased muscle regenerative potential contributed to diaphragm muscle hypertrophy, whereas decreased protein synthesis, increased protein degradation and decreased muscle regenerative potential contributed to gastrocnemius muscle atrophy. In conclusion, the differences in muscle regeneration and regulatory pattern of protein metabolism might contribute to the different adaptive changes observed in the diaphragm and gastrocnemius muscles of ground squirrels.

Different protein metabolic strategies for growth during food-induced physiological plasticity in echinoid larvae.

Food-induced morphological plasticity, a type of developmental plasticity, is a well-documented phenomenon in larvae of the echinoid echinoderm, Dendraster excentricus A recent study in our lab has shown that this morphological plasticity is associated with significant physiological plasticity for growth. The goal of the current study was to measure several aspects of protein metabolism in larvae growing at different rates to understand the mechanistic basis for this physiological growth plasticity. Larvae of D. excentricus were fed rations of 1000 algal cellsml-1 (low-fed larvae) or 10,000 algal cellsml-1 (high-fed larvae). Relative protein growth rate was 6.0 and 12.2%day-1 for low- and high-fed larvae, respectively. The energetic cost of protein synthesis was similar for the two treatments at 4.91 J mg-1 protein synthesized. Larvae in both treatments used about 50% of their metabolic energy production to fuel protein synthesis. Mass-specific rates of protein synthesis were also similar. Large differences in mass-specific rates of protein degradation were observed. Low-fed larvae had relatively low rates of degradation early in development that increased with larval age, surpassing those of high-fed larvae at 20 days post-fertilization. Changes in protein depositional efficiency during development were similar to those of larval growth efficiency, indicating that differences in protein metabolism are largely responsible for whole-organism growth plasticity. Low-fed larvae also had alanine transport rates that were 2 times higher than those of high-fed larvae. In total, these results provide an explanation for the differences in growth efficiency between low- and high-fed larvae and allow for a more integrated understanding of developmental plasticity in echinoid larvae.

Triploidy affects postprandial ammonia excretion but not specific dynamic action in 1+ brook charr, Salvelinus fontinalis

The use of sterile triploids in aquaculture is currently limited because of reduced performance in situations of aerobic stress such as high temperature, hypoxia, and exhaustive exercise. Many studies have therefore attempted to find underlying metabolic differences between triploids and their diploid counterparts to improve triploid rearing protocols. This study investigated the effects of triploidy on postprandial metabolism (and therefore also pre-feeding standard metabolic rate; SMR) in 1+ (193 ± 5 g; mean ± SE) diploid and triploid brook charr, Salvelinus fontinalis. Oxygen uptake and total ammonia nitrogen (TAN) excretion were measured at 14–15 °C for previously fasted (for eight days) fish from 48 h before to 48 h after being sham-fed or fed a single ration of 0.4% of their body mass. While there were no ploidy-related differences in SMR or any of the measured SDA parameters, triploids had significantly higher net TAN excretion (by 36%) than diploids. Such a difference in protein metabolism could affect triploid growth and survival when simultaneously faced with suboptimal conditions, though further studies are warranted.

Relationship between feed efficiency indexes and thermography, blood, and ruminal parameters in pre-weaning dairy heifers.

The objective of this study was to evaluate whether pre-weaning heifer calves divergent for residual feed intake (RFI) or residual feed intake and body weight gain (RIG) exhibit differences in thermography, blood, and ruminal parameters. Thirty-two Gyr heifer calves were enrolled in a 63-d trial and classified into 2 feed efficiency (FE) groups based on RFI and RIG (mean ± 0.5 SD). The groups were classified as high efficiency (HE) RFI (HE RFI, n = 9), HE RIG (HE RIG, n = 10), low efficiency (LE) RFI (LE RFI, n = 10), and LE RIG (LE RIG, n = 11). The amount of whole milk provided for each calf was calculated based on their metabolic weight at birth (42% x BW0.75). The liquid diet was divided into two meals at 0700 and 1400 h. The total solid diet (TSD) was composed of 92% concentrate and 8% of Tifton 85 hay chopped in 5-cm lengths, as fed. Intake was measured daily. Blood concentrations of insulin, beta hydroxybutyrate, urea, and glucose, and ruminal pH, N-NH3, and volatile fatty acids (VFA) were evaluated at 14, 28, 42, 56, and 70 days of age. Thermal images of the calves were taken with an infrared camera (FLIR T420, FLIR Systems Inc., Wilsonville, OR) on d 56 (±3) at 0600 h, before the morning feeding. Total VFA concentration and propionate as % of total VFA were 24.2% and 22.2% lower in HE RFI compared to LE RFI calves, respectively. On the other hand, acetate as % of total VFA was 10.6% greater in HE RFI than LE RFI calves. Blood urea concentration tended to be greater in LE RFI than HE RFI calves. High efficiency HE RIG tended to have 6.8% greater acetate and 15.4% lower propionate as % of total VFA than LE RIG. Blood insulin concentration was greater and blood glucose tended to be greater for LE RIG than HE RIG group. Low efficiency RIG group had greater left rib, left flank, and anus surface temperature measured by infrared thermography than the HE RIG group. Differences in ruminal fermentation do not seem to be associated with pre-weaning calves efficiency, while differences in protein metabolism seem to affect RFI during this phase. Infrared thermography appears to be correlated to RIG in pre-weaning heifer calves.

Atypical structural tendencies among low-complexity domains in the Protein Data Bank proteome.

A variety of studies have suggested that low-complexity domains (LCDs) tend to be intrinsically disordered and are relatively rare within structured proteins in the Protein Data Bank (PDB). Although LCDs are often treated as a single class, we previously found that LCDs enriched in different amino acids can exhibit substantial differences in protein metabolism and function. Therefore, we wondered whether the structural conformations of LCDs are likewise dependent on which specific amino acids are enriched within each LCD. Here, we directly examined relationships between enrichment of individual amino acids and secondary structure tendencies across the entire PDB proteome. Secondary structure tendencies varied as a function of the identity of the amino acid enriched and its degree of enrichment. Furthermore, divergence in secondary structure profiles often occurred for LCDs enriched in physicochemically similar amino acids (e.g. valine vs. leucine), indicating that LCDs composed of related amino acids can have distinct secondary structure tendencies. Comparison of LCD secondary structure tendencies with numerous pre-existing secondary structure propensity scales resulted in relatively poor correlations for certain types of LCDs, indicating that these scales may not capture secondary structure tendencies as sequence complexity decreases. Collectively, these observations provide a highly resolved view of structural tendencies among LCDs parsed by the nature and magnitude of single amino acid enrichment.

Determinants and Regulation of Protein Turnover in Yeast.

Protein turnover maintains the recycling needs of theproteome, and its malfunction has been linked to aging and age-related diseases. However, not allproteins turnover equally, and the factors that contribute to accelerate or slow down turnover are mostly unknown. We measured turnover rates for 3,160 proteins in exponentially growing yeast and analyzed their dependence on physical, functional, and genetic properties. We found that functional characteristics, including protein localization, complex membership, and connectivity, have greater effect on turnover than sequence elements. We also found that protein turnover and mRNA turnover are correlated. Analysis under nutrient perturbation and osmotic stress revealed that protein turnover highly depends on cellular state and is faster when proteinsare being actively used. Finally, stress-induced changes in protein and transcript abundance correlated with changes in protein turnover. This study provides a resource of protein turnover rates and principles to understand the recycling needs of the proteome under basal conditions and perturbation.

12 Differences between overweight/obese patients and normal weight patients with stable heart failure. A possible link to survival benefit

Background Chronic heart failure (CHF) patients with increased body mass index (BMI) (overweight and obese) have better survival rates than their normal weight counterparts. There is no clear consensus about what factors-related to increased BMI underlie this benefit hence more studies are encouraged. Objective We aimed to assess how body composition, diet, exercise levels and functional capacity differed between obese/overweight and normal weight patients. Methods 106 patients with chronic stable heart failure were categorised according to their BMI. Anthropometric measurements, body composition (Tanita), fat mass and free Fat mass, dietary 3 day records and functional assessments including 6 minute walk test, 3 day FitBit records and NYHA class were recorded (Table 1). Results There were no differences in daily energy, fat, or carbohydrate consumption or activity levels between the groups. However, overweight and obese CHF patients have significantly increased fat mass, % fat, free fat mass and consumed more protein. Mean age 66.8 ± 12.7. 72.2% (n = 78) were male and 27.8% (n = 30) were female. Conclusion Our study highlights that energy intake and activity levels do not differ significantly between overweight/obese and normal weight patients. However, the higher fat free mass and protein intake suggests differences in protein metabolism may be relevant to the survival benefit with higher BMI.

Selection for residual feed intake affects appetite and body composition rather than energetic efficiency

Residual feed intake (RFI) is the difference between an animal’s actual feed intake and that which would be expected based on production. This experiment was to test the hypothesis that part of the variation in RFI may be due to differences in energetic efficiency through changes in heat production, these being in part due to differences in protein metabolism. Following three generations of divergent selection for RFI, eight High and eight Low-RFI heifers were fed at both 105% and 180% of predicted maintenance feed requirements. Between-RFI line and feeding-level differences were assessed for energy intake, protein metabolism, heat production, body composition, energy and nitrogen balance and digestibility. The RFI lines did not differ in protein metabolism or heat production. The High-RFI heifers deposited 51% and 56% more subcutaneous fat at the P8 rump and 12/13th rib sites, respectively, with no difference in eye muscle area gain or average daily weight gain. The greater fat deposition of High-RFI heifers was due to a larger ad libitum feed consumption compared with the Low-RFI heifers. Energy and nitrogen balance did not differ between the RFI lines. The energy transactions indicated no difference in the efficiency of energy use on 105% maintenance, although when fed 180% of maintenance the differences in feed intake suggest variation in appetite as the mechanism contributing to RFI. All of the extra energy consumed by High-RFI heifers above maintenance and deposition of protein was associated with additional energy retained as fat. This study suggests that selection for RFI may not lead to improved efficiency of energy use.

Genetic Strain-Dependent Protein Metabolism and Muscle Hypertrophy Under Chronic Isometric Training in Rat Gastrocnemius Muscle

Genetic strain-dependent reactivity to mechanical stimuli in rat skeletal muscle has not been examined. This study aimed to examine whether genetic strain-dependency is associated with reactivity in protein metabolism and the resultant muscle hypertrophy after isometric resistance training (RT). The right triceps of Sprague-Dawley (SD) and Wistar rats underwent 12 sessions of RT. After RT, a transition from the IIb to the IIx myosin heavy-chain isoform was observed in both strains. In SD rats, the lateral gastrocnemius muscle (LG) mass of the trained legs (TRN) was significantly higher than that of the control legs (CON) (7.8 %, P<0.05). Meanwhile, in Wistar rats, the LG mass was unchanged. In SD rats, the levels of 70-kDa ribosomal protein S6 kinase (p70S6k) and forkhead box 3a (FOXO3a) phosphorylation in the TRN were significantly greater than those of the CON (2.2- and 1.9-fold, respectively; P<0.05). The expression of muscle ring finger-1 (MuRF1) and muscle atrophy F-box (MAFbx/atrogin-1) in the TRN were significantly lower than those of the CON (0.6- and 0.7-fold, respectively; P<0.05). However, in Wistar rats, there was no significant difference. These results suggest a genetic strain difference in protein metabolism. This phenomenon may be useful for studying individual differences in response to RT.

Protein metabolism in women and men: similarities and disparities

To provide an objective and comprehensive review of the recent literature addressing the effects of sex on protein metabolism. We also evaluate whether these differences can be attributed to physiology or methodology. Because of the developmental changes in hormonal milieu and body composition that occur across life, the literature has been examined in a longitudinal manner across the lifespan. Throughout most points of life, men and women of similar health status and BMI display fairly similar protein turnover rates. However, some investigators have reported sexual dimorphism in protein metabolism, which may be partly attributable to differences in fat-free mass and/or methodology. In periods of significant changes in the hormonal milieu (puberty and menopause), sex differences may become more evident. Finally, anabolic stimuli such as feeding and exercise may help highlight any discrepancies in protein turnover between men and women. Sex differences in protein metabolism, if any, are most evident during the main phases of hormonal changes, and may be also due to differences in body composition. However, methodological issues and sample size must be considered when designing and evaluating these studies.

A unifying theory for general multigenic heterosis: energy efficiency, protein metabolism, and implications for molecular breeding

Hybrids between genetically diverse varieties display enhanced growth, and increased total biomass, stress resistance and grain yield. Gene expression and metabolic studies in maize, rice and other species suggest that protein metabolism plays a role in the growth differences between hybrids and inbreds. Single trait heterosis can be explained by the existing theories of dominance, overdominance and epistasis. General multigenic heterosis is observed in a wide variety of different species and is likely to share a common underlying biological mechanism. This review presents a model to explain differences in growth and yield caused by general multigenic heterosis. The model describes multigenic heterosis in terms of energy-use efficiency and faster cell cycle progression where hybrids have more efficient growth than inbreds because of differences in protein metabolism. The proposed model is consistent with the observed variation of gene expression in different pairs of inbred lines and hybrid offspring as well as growth differences in polyploids and aneuploids. It also suggests an approach to enhance yield gains in both hybrid and inbred crops via the creation of an appropriate computational analysis pipeline coupled to an efficient molecular breeding program.

Integrated multilaboratory systems biology reveals differences in protein metabolism between two reference yeast strains

The field of systems biology is often held back by difficulties in obtaining comprehensive, high-quality, quantitative data sets. In this paper, we undertook an interlaboratory effort to generate such a data set for a very large number of cellular components in the yeast Saccharomyces cerevisiae, a widely used model organism that is also used in the production of fuels, chemicals, food ingredients and pharmaceuticals. With the current focus on biofuels and sustainability, there is much interest in harnessing this species as a general cell factory. In this study, we characterized two yeast strains, under two standard growth conditions. We ensured the high quality of the experimental data by evaluating a wide range of sampling and analytical techniques. Here we show significant differences in the maximum specific growth rate and biomass yield between the two strains. On the basis of the integrated analysis of the high-throughput data, we hypothesize that differences in phenotype are due to differences in protein metabolism.

The balancing act between the cellular processes of protein synthesis and breakdown: exercise as a model to understand the molecular mechanisms regulating muscle mass

the more we learn about skeletal muscle biology the more it becomes clear how important muscle tissue is for maintaining overall health. Specifically, muscle is important to provide sufficient strength/force production for locomotion and performing regular activities associated with daily living. In

Human amyloid-β synthesis and clearance rates as measured in cerebrospinal fluid in vivo

Certain disease states are characterized by disturbances in production, accumulation or clearance of protein. In Alzheimer disease, accumulation of amyloid-beta (Abeta) in the brain and disease-causing mutations in amyloid precursor protein or in enzymes that produce Abeta indicate dysregulation of production or clearance of Abeta. Whether dysregulation of Abeta synthesis or clearance causes the most common form of Alzheimer disease (sporadic, >99% of cases), however, is not known. Here, we describe a method to determine the production and clearance rates of proteins within the human central nervous system (CNS). We report the first measurements of the fractional production and clearance rates of Abeta in vivo in the human CNS to be 7.6% per hour and 8.3% per hour, respectively. This method may be used to search for novel biomarkers of disease, to assess underlying differences in protein metabolism that contribute to disease and to evaluate treatments in terms of their pharmacodynamic effects on proposed disease-causing pathways.

Protein kinetic differences between children with edematous and nonedematous severe childhood undernutrition in the fed and postabsorptive states

Pathogenic factors that cause a child to develop the edematous instead of the nonedematous form of severe childhood undernutrition (SCU) during food deprivation are not clear. It was hypothesized that, in edematous but not nonedematous SCU, impaired protein breakdown leading to inadequate amino acids for maintenance of important organ systems was a factor. We measured protein kinetics in children with edematous and nonedematous SCU. Endogenous leucine flux, an index of whole-body protein breakdown rate, was determined in 4 groups of children with edematous or nonedematous SCU in the malnourished and recovered states. Two groups were studied in the postabsorptive state, and 2 groups were studied in the fed state. In the postabsorptive state, leucine flux was slower (P < 0.01) in the edematous group than in the nonedematous group in the malnourished state, but in the recovered state, it was faster (P < 0.05) in the children who previously had edematous SCU. When compared with the malnourished state value, leucine flux at recovery doubled in the group that previously had edematous SCU, but it did not change in the other group. In the fed state, leucine flux was slower (P < 0.01) in the edematous group than in the nonedematous group in the malnourished state but not in the recovered state. In the recovered state, enteral leucine extraction by splanchnic tissues trended higher in the group that previously had edematous SCU than in the nonedematous group. These findings indicate different protein breakdown responses to food deprivation between children with edematous and nonedematous SCU and inherent differences in protein metabolism when they have recovered.

Arginine and Mixed Amino Acids Increase Protein Accretion in the Growth-Restricted and Normal Ovine Fetus by Different Mechanisms

Protein metabolism may be perturbed in intrauterine growth restriction (IUGR). Arginine is indispensable for growth and nitrogen balance in young mammals. Fetuses with IUGR therefore may benefit from arginine supplementation. The purpose of this study was to determine 1) the effects of IUGR on protein metabolism in the ovine fetus and 2) the effects of arginine or mixed amino acid (AA) infusion on protein metabolism in these fetuses. Pregnant ewes and their fetuses were catheterized at 110 d gestation and randomly assigned to control or IUGR groups. IUGR was induced by repetitive placental embolization. Parameters of fetal protein metabolism were determined from [ring-(2)H(5)]phenylalanine kinetics at baseline and in response to a 4-h infusion of either arginine or an isonitrogenous AA mixture. There were no differences in protein metabolism between control and IUGR groups either at baseline or in response to arginine or AA treatment. Both arginine and AA infusion increased fetal protein accretion in both groups. Arginine did this by decreasing protein turnover, synthesis, and breakdown. AAs increased protein turnover and synthesis while decreasing protein breakdown. AA infusion resulted in a significantly higher increase in protein accretion than arginine infusion. Thus, in the ovine fetus, placental embolization has no clear effect on protein metabolism. Arginine and AAs both stimulate protein accretion but do so in distinctly different ways. Mixed AA infusion has a greater effect on protein accretion than arginine alone and therefore may be a better strategy for stimulating fetal growth.

Human soleus and vastus lateralis muscle protein metabolism with an amino acid infusion

The calf muscles, compared with the thigh, are less responsive to resistance exercise in ambulatory and bed-rested individuals, apparently due to muscle-specific differences in protein metabolism. We chose to evaluate the efficacy of using amino acids to elevate protein synthesis in the soleus, because amino acids have been shown to have a potent anabolic effect in the vastus lateralis. Mixed muscle protein synthesis in the soleus and vastus lateralis was measured before and after infusion of mixed amino acids in 10 individuals (28 +/- 1 yr). Phosphorylation of ribosomal protein p70 S6 kinase (p70S6K; Thr389) and eukaryotic initiation factor 4E-binding protein-1 (4E-BP1; Thr37/46) was also evaluated at rest and after 3 h of amino acid infusion. Basal protein synthesis was similar (P = 0.126), and amino acids stimulated protein synthesis to a similar extent (P = 0.004) in the vastus lateralis (0.043 +/- 0.011%/h) and soleus (0.032 +/- 0.017%/h). Phosphorylation of p70S6K (P = 0.443) and 4E-BP1 (P = 0.192) was not increased in either muscle; however, the soleus contained more total (P = 0.002) and phosphorylated (P = 0.013) 4E-BP1 than the vastus lateralis. These data support the need for further study of amino acid supplementation as a means to compensate for the reduced effectiveness of calf resistance exercise in ambulatory individuals and those exposed to extended periods of unloading. The greater 4E-BP1 in the soleus suggests that there is a muscle-specific distribution of general translational initiation machinery in human skeletal muscle.

Gender differences in protein metabolism.

Adult male and female humans have clear differences in muscle mass and there is mounting evidence that substrate metabolism differs between genders. These facts suggest that there are gender differences in protein metabolism between males and females. Studies utilizing stable isotopically labeled amino acids show little indication that whole body protein synthesis or breakdown is different between genders. There is evidence that leucine oxidation may be different, both at rest and during exercise, but this evidence is not unequivocal and more, properly controlled studies need to be undertaken to clarify this controversy. Muscle hypertrophy results from positive net muscle protein balance, thus, adult males must have greater net muscle protein synthesis than females, at least at some point in development. Although there is a paucity of data, no gender differences in the basal level net muscle protein balance have been found. It is possible that there are small differences that cannot be distinguished with current methods due to small sample sizes and the sensitivity of the methods. It is more likely, however, that sex hormones contribute to the clear differences in musculature by influencing muscle protein metabolism, especially during puberty. Testosterone increases muscle protein synthesis and net muscle protein balance, resulting in increased muscle mass. Males and females have similar amounts of testosterone until puberty, then testosterone levels increase much more dramatically in males, as does muscle mass. Furthermore, although no evidence exists in humans, in-vitro and rat data suggest that ovarian hormones inhibit muscle protein synthesis. Whereas solid conclusions are difficult to make given the paucity of studies focusing on gender differences in human protein metabolism, it seems that the sex hormones may play an important role. Certainly, more studies need to be conducted to ascertain what gender differences in whole body and muscle protein metabolism exist and how these differences result in different phylogenetic characteristics.

Population responses to contaminant exposure in marine animals: influences of genetic diversity measured as allozyme polymorphism.

Current understanding of the genetic and metabolic basis of relations between heterozygosity and animal performance under non-polluted conditions is relevent to interpreting apparently inconsistent findings concerning the relative advantage of allozyme polymorphism during contaminant exposure. Many interrelated factors which may influence and even compromise those relations include species (lifestyle, reproductive behaviour etc), lifestage, environmental influences and a variety of background genetic effects (limited parentage, null alleles, aneuploidy, genomic imprinting etc). Nevertheless, there is some promise that single-locus responses may be diagnostic for specific pollutants. In addition, limited evidence to date supports the a priori expectation that reduced energy requirements for maintenance metabolism may facilitate longer survival of multiple-locus heterozygotes during exposure to contaminants with toxic effects that result either in the reduced acquisition or availability of metabolizable energy, and/or a reduction in the efficiency with which metabolizable energy is used to fuel metabolic processes. More work is required to fully establish this trend in response to specific contaminant types, to assess any direct consequences of underlying differences in protein metabolism, and to resolve the interactive effects of contaminant mixtures. But the functional value of genetic variation within populations is confirmed. Reduced genetic diversity may not only compromise the capacity of an impacted population for genetic adaptation in the face of further environmental challenge, but may also result in increased energy requirements, lower production efficiency and reduced reproductive output. These metabolic consequences of reduced genetic polymorphism would further lower that population's potential for survival under lethal conditions of contaminant exposure, and also affect the genetic makeup of populations through differential reproduction under conditions of sublethal stress.