Differences In Hematocrit Levels Research Articles

Hematocrit and Hemoglobin Levels in Pregnant Women with Preeclampsia in Palembang City

Preeclampsia is a collection of symptoms that arise in pregnant, maternity, and postpartum women in the form of hypertension, edoema, and proteinuria that appear in the 20th week of pregnancy until the end of the first week after delivery. Several parameters of hematological examination in pregnant women are hematocrit and hemoglobin levels, which are suspected to be different between normal pregnant women and pregnant women who have preeclampsia. This study aims to determine the levels of hematocrit and hemoglobin in pregnant women with preeclampsia at RSIA Rika Amelia Palembang in 2021. This research is descriptive in nature, with a total sample size of 105 patients. The results of the study showed that the average hematocrit level in preeclamptic pregnant women was 37% and the hemoglobin level was 12 g/dl. Statistical tests showed no difference in hematocrit levels based on the mother's age (p-value = 0.678) or gestational age (p-value = 0.226). Meanwhile, for the hemoglobin level parameter, statistical tests showed no difference in hemoglobin levels based on the mother's age (p-value = 0.386) or gestational age (p-value = 0.104). It can be concluded that there is no difference in hematocrit and hemoglobin levels in preeclamptic pregnant women based on age and gestational age.

Effect of Additional Iron Injection to Suckling Pigs on Hematocrit Level during the Suckling Period.

Two experiments were conducted to evaluate the effects of additional iron injection to suckling piglets on hematocrit level during the suckling period. In Experiment 1, a total of 24 piglets were allotted into 3 treatments within litter based on body weight and sex at d 1 of age. Treatments were: (1) Control: a 200 mg iron-dextran intramuscular injection only at d 0 of experiment (d 1 of age), (2) Iron100: intramuscular iron-dextran injections at d 0 (200 mg iron) and 15 (11 d before weaning) of experiment (100 mg iron), and (3) Iron200: intramuscular iron-dextran injections at d 0 (200 mg iron) and 15 of experiment (200 mg iron). In Experiment 2, a total of 20 piglets were allotted into 2 treatments within litter based on body weight and sex at d 1 to 2 of age. Treatments were: (1) Iron100b: 100 mg iron-dextran intramuscular injection at d 0 of experiment (d 1 to 2 of age), and (2) Iron200b: 200 mg iron-dextran intramuscular injection at d 0 of experiment. An additional 200 mg iron-dextran was injected to all piglets intramuscularly at d 14 of experiment (11 d before weaning). In Experiment 1, there was no difference in hematocrit levels among treatments at d 15 of experiment. Both iron treatments had greater hematocrit levels than the Control treatment at d 22 and 26 of experiment (p < 0.05). The Iron200 treatment had greater hematocrit level at d 26 of experiment (p < 0.05) and tended to have a greater increase of hematocrit levels in d 22 to 26 of experiment (p = 0.09) than the Iron100 treatment. In Experiment 2, the Iron200b treatment had greater hematocrit levels than the Iron100b treatment in d 14 and 25 (p < 0.05) of experiment. Hematocrit level changes tended to be greater in the Iron200b treatment in d 0 to 14 (p = 0.08) of experiment but lower in d 14 to 25 (p < 0.05) of experiment than the Iron100b treatment. The additional iron injection to suckling piglets increased hematocrit levels with greater values at weaning in the higher injection level whereas the increase was greater when the hematocrit level was low at the time of additional injection.

Modeling the Effect of Hyperoxia on the Spin-Lattice Relaxation Rate R1 of Tissues.

PurposeInducing hyperoxia in tissues is common practice in several areas of research, including oxygen‐enhanced MRI (OE‐MRI), which attempts to use the resulting signal changes to detect regions of tumor hypoxia or pulmonary disease. The linear relationship between PO2 and R1 has been reproduced in phantom solutions and body fluids such as vitreous fluid; however, in tissue and blood experiments, factors such as changes in deoxyhemoglobin levels can also affect the ΔR1.Theory and MethodsThis manuscript proposes a three‐compartment model for estimating the hyperoxia‐induced changes in R1 of tissues depending on B0, SO2, blood volume, hematocrit, oxygen extraction fraction, and changes in blood and tissue PO2. The model contains two blood compartments (arterial and venous) and a tissue compartment. This model has been designed to be easy for researchers to tailor to their tissue of interest by substituting their preferred model for tissue oxygen diffusion and consumption. A specific application of the model is demonstrated by calculating the resulting ΔR1 expected in healthy, hypoxic and necrotic tumor tissues. In addition, the effect of sex‐based hematocrit differences on ΔR1 is assessed.ResultsThe ΔR1 values predicted by the model are consistent with reported literature OE‐MRI results: with larger positive changes in the vascular periphery than hypoxic and necrotic regions. The observed sex‐based differences in ΔR1 agree with findings by Kindvall et al. suggesting that differences in hematocrit levels may sometimes be a confounding factor in ΔR1.ConclusionThis model can be used to estimate the expected tissue ΔR1 in oxygen‐enhanced MRI experiments.

Exercise and the Effects of Hydration on Blood Viscosity in Sickle Cell Trait Carriers: A Critically Appraised Topic

Clinical Question: During bouts of exercises in a hypohydrated state, do patients with sickle cell trait compared with patients without sickle cell trait demonstrate higher blood viscosity? Clinical Bottom Line: There is evidence that there is a difference in hematocrit levels in patients with sickle cell trait and patients without sickle cell trait both pre- and postexercise with suboptimal hydration status. Moreover, the effects on hematocrit levels increase over baseline levels in the recovery stages postexercise in patients with sickle cell trait.

Comparative Analysis of Beet Juice and Red Guava Juice against Erythrocyte and Hematocrit Levels in Post-partum Women

BACKGROUND: Post-partum physiological bleeding can cause post-partum mothers to experience anemia; often, post-partum mothers are limited by nutrient and fluid intake to keep the body strong and have ideal body weight. AIM: The aim of the study is to knowing the difference between beet juice and Guava juice on erythrocyte and hematocrit (HTC) levels in post-partum mothers at Darul Imarah Health Center, Darul Imarah District, Aceh Besar District. METHODS: This was a quasi-experimental study with a pre-post test design using a randomized controlled trial. The population of post-partum mothers in the Darul Imarah Health Center, Darul Imarah District, Aceh Besar District. The sample selection is purposive sampling with inclusion and exclusion criteria; the sample is 45 people. RESULTS: The results showed differences in erythrocyte and HTC levels between before and after giving guava and iron (Fe) juices with a p-value of 0.00 <0.05. Beet juice increases the average erythrocytes and HTC levels higher than guava juice. There were significant differences in erythrocyte levels in the guava + Fe, beet + Fe, and control (Fe) treatment groups with a p-value of 0.001 <0.05. the difference in the average difference in erythrocyte levels between the guava and beet treatment groups was 0.03. The treatment group Bit + Fe had a higher mean erythrocyte level different than the guava + the treatment group and the control group (Fe). There was a significant difference in HTC levels in the guava + Fe, beet + Fe, and control (Fe) treatment groups with a p-value of 0.001 <0.05. the difference in the average difference in erythrocyte levels between the guava and beet treatment groups was 0.03. The treatment group Bit + Fe had a higher mean difference in HTC levels than the guava + the treatment group and the control group (Fe). CONCLUSION: There is a significant difference in erythrocytes and HTC levels between before and after giving guava juice and beet juice to post-partum mothers with a p-value of 0.00 <0.05. Beet juice increases the average level of erythrocytes and HTC, which is higher than guava juice in post-partum mothers at the Darul Imarah Health Center.

Melatonin prevented the elevation of leukocyte count and the decreased of hematocrit levels in burn-induced Wistar Rats

Background: Burns are commonly caused by thermal injuries that are often encountered in everyday life. Burns may cause tissue damage and tissue infection. Melatonin, a hormone that acts as an antioxidant and anti-inflammatory, can be used as a supportive therapy in patients with burns by preventing excess tissue damage, decreasing inflammatory response, and preventing extreme leukocyte increase. The study aims to investigate the effects of melatonin supplementation on leukocyte count and hematocrit levels in male Wistar rats with burns injury.Method: This was an experimental animal study with randomized control group design. A total of twelve healthy male Wistar rats were randomized and divided into two groups. All Wistar rats were induced with 30% burns injuries area. The melatonin group was intraperitoneally administered with melatonin at 0, 8, and 16 hours after treatment, while the control group was administered with placebo (aquadest) with similar method. Leukocyte count and hematocrit levels were measured by taking retroorbital venous blood at 0, 3, and 24 hours after treatment. Data were analyzed with Paired t-Test, Independent t-Test, Mann-Whitney, or Wilcoxon test depend on their normality.Results: There was a higher leukocyte count in control group compared to melatonin group at 3 hours after treatment (T3) (p=0.050), but not at 0 hours after treatment (T0) and at 24 hours after treatment (T24) (p>0.05). There was increased leukocyte count in both melatonin and control groups in T3 in comparison with T0, however the increment was steeper in control group compared to melatonin group. There were no significant differences in hematocrit levels between the melatonin and control groups in T0, T3, and T24 (p>0.05). There was a steeper increased hematocrit levels in the control group than the melatonin group after T24, although it was not statistically significant.Conclusion: Melatonin might have role in preventing a steeper rise of leukocyte count and hematocrit levels in burns injury. If confirmed by further studies, melatonin might be potential for adjuvant management in burns.

Performance evaluation of the ZL 6000i cone-plate rotational viscometer

Elevated whole blood viscosity (WBV) is associated with increasing blood flow resistance in the microcirculation and is used to predict the occurrence and outcome of cardiovascular diseases. We evaluated the analytical performance of the ZL 6000i (Zonci Technology, Beijing, China), new fully automated cone-plate rotational type viscometer. We collected blood samples from 287 adults to establish a reference range. We evaluated total precision for 20 days using quality control (QC) samples and within-run precision was evaluated by performing 50 consecutive measurements of blood samples. Aliquots of blood samples were stored at room temperature and 5°C to assess the effect of storage time and temperature on viscosity measurement. The reference ranges and median values of WBV were significantly higher in males than in females due to difference in hematocrit level. The coefficients of variation (CVs) for precision using QC and human blood samples were < 7.5% at all shear rates. WBV of the samples stored at room temperature for up to 6 h, and at 5°C for samples stored up to 2 days was not different from the control. In this study, the ZL 6000i viscometer yields reproducible WBV data and is clinically useful for monitoring viscosity.

PENGARUH HIJAMAH TERHADAP KADAR ERITROSIT DAN HEMATOKRIT DARAH VENA ORANG SEHAT

Hijamah is a treatment technique, based on the tradition that has been practiced by humans since time immemorial, now the treatment has been modernized and adjusted and follows scientific principles by using practical, sterile and effective tools, hijamah is done to remove blood dangerous from inside the body through puncture on the surface of the skin, the aim of the study was to determine the effect of hijamah on erythrocyte levels and hematocrit of venous blood of healthy people. South while blood collection was carried out at the South Sumatra Provincial Center for Health Laboratory (BBLK), the study sample was 30 people with healthy conditions, aged 18 to 22 years, this study was conducted 8 hours after hijamah treatment, the results of the study were significant differences after doing hijamah on venous blood erythrocyte levels of healthy people with a p value of 0.012, while at hematocrit levels there was a statistically significant difference in hematocrit levels of healthy venous blood with p value 0.001. Hijamah affects levels of erythrocytes and hematocrit, hijamah can be used in alternative and complementary medicine. Keywords: Hijamah. Erythrocytes, Hematocrit

THE EFFICACY OF DUTASTERIDE AND GREEN TEA TOWARDS BLEEDING ON BPH AFTER TURP: STUDY THEIR EFFECT ON HIF-1α EXPRESSION &amp; HEMATOCRIT

Objective: This study proves differentiation of the combination of dutasteride and green tea, dutasteride, green tea, and placebo alone and their association with differences in hematocrit levels and the expression of hypoxia induced factor-1 alpha (HIF-1α) in patients with Benign Prostate Hyperplasia (BPH) were performed Trans urethral resection of the prostate (TURP) surgery. Material &amp; method: Experimental study with the draft "randomized control trial". Comparing angiogenesis changes between groups of BPH patients who underwent TURP surgery to assess the expression of HIF-1α and Δ Ht (Hematocrit) after administration of dutasteride, green tea with combination of dutasteride and green tea for 14 days. Results: The combination of dutasteride and green tea was not significant in reducing the expression of HIF-1α. Mean P1 group (59.32 ± 14.69); P2 group (59.11 ± 20.73); P3 group (64.21 ± 14.95); K group (58.16 ± 16.00). Kruskal test results obtained p=0.491 walis which means the difference percentage of HIF-1α among the 4 groups was not significant. The mean Δ Ht P1 group (0.61 ± 0.204); P2 group (0.54 ± 0.250); P3 group (0.41 ± 0.275); group K (0.41 ± 0.275). In statistical test with Mann Whitney test comparing the percentage reduction obtained Ht levels dutasteride group against group of green tea obtained p=0.213 means that there is no significant difference. Where a significant difference to the other groups. Conclusion: The combination of dutasteride and green tea for 14 days before TURP surgery does not reduce the expression of HIF-1α in BPH patients who underwent TURP surgery. Δ Ht significant decline in the combination group compared with other groups and might be influenced by several factors during TURP surgery.

스쿠버 운동 시 서로 다른 호흡 산소 분압 차이가 헤마토크리트 변화에 미치는 영향

This research examined the effect of oxygen partial pressure on hematocrit changes in SCUBA diving exercise Ten male university students divers performed cycle exer cise on immersion at 5 m and a pedaling of stationary bike for 20 min. Blood was drown from ante cubital v ein at resting, immediately after exercise, and 30min after that. No significant differences was found in circulating red blood c ell, mean corpuscular volume and mean cor -puscular hemoglobin concentration among the three experimental conditions. There were significant differences in hematocrit levels, hemoglobin, and mean corpuscular hemoglobin among the three different experimental conditions. In conclusion, The hyperbaric hyperoxia condition during cycle exercise on immersion was found to effect the hematocrit and hemoglobin. The phenomenon maybe related to hyperbaric hyperoxia condition. The hyperbaric hyperoxia condition can lead to improve diffusive oxyg en transport as indicated by the improvement of local tissue oxygen partial pressure in the absence of a sig nificant change in total oxygen delivery capacity. Our results indicate that exercise in the hyperbaric hyperoxia condition like scuba diving might be play a role in the change the blood profile and hemodynamics.

Xenogeneic transplantation of erythropoietin-secreting cells immobilized in microcapsules using transient immunosuppression

Cell encapsulation technology holds promise for the sustained and controlled delivery of therapeutic proteins such as erythropoietin (Epo). Transplantation of microencapsulated C 2C 12 myoblasts in syngeneic and allogeneic recipients has been proven to display long-term survival when implanted subcutaneously. However, xenotransplantation approaches may be affected by the rejection of the host and thus may require transient immunosuppression. C 2C 12 myoblasts genetically engineered to secrete murine Epo (mEpo) were encapsulated in alginate-poly- L-lysine-alginate (APA) microcapsules and implanted subcutaneously in Fischer rats using a transient immunosuppressive FK-506 therapy (2 or 4 weeks) to ameliorate immunoprotection of microcapsules. Rats receiving short-term immunosupression with FK-506 maintained high hematocrit levels for a longer period of time (14 weeks) in comparison with the non-immunosuppressed group. In addition, a significant difference in hematocrit levels was detected by day 65 among rats immunosuppressed for 2 or 4 weeks, corroborating the need of a minimum period of immunosuppression (4 weeks) for this purpose. These results highlight the importance of applying a minimum period (4 weeks) of transient immunosuppression if the host acceptance of xenogeneic implants based on microencapsulated Epo-secreting cells is aimed.

Casting Doubt on the Safety of “Off-the-shelf” Mesenchymal Stem Cells for Cell Therapy

Mesenchymal stem cells (MSCs) show promise for gene delivery to treat various diseases such as anemia and stroke, as well as other oncological and neural disorders.1,2 In this issue of Molecular Therapy, Campeau et al.3 report on an experimental model that compares the responses of allogeneic and syngeneic hosts to the transfer of erythropoietin (EPO)-expressing MSCs. The studies were based on the premise that EPO could be delivered using genetically modified MSCs to treat anemia or myocardial infarction. The treatment of anemia with expanded autologous MSCs seems plausible, in that the chronic nature of anemia is compatible with the time needed to expand bone marrow−derived MSCs to sufficient numbers. However, in the case of acute disorders such as myocardial infarction and stroke,4,5 gene delivery interventions would have to be immediate, thereby eliminating autologous gene-modified MSCs as an option. Because MSCs have been reported to suppress allogeneic responses, in particular graft-versus-host disease,6,7,8 “off-the-shelf” sources of such cells have been proposed to treat various clinical disorders that require intervention at early time points.

Effect of photoperiod manipulation on the growth performance and stress response of juvenile red sea bream ( Pagrus major)

Juvenile of red sea bream ( Pagrus major, body weight 1∼30 g) were reared under four photoperiods (6L:6D, 12L:12D, 16L:8D and 24L:0D) with constant light intensity 1500 lx on the water surface to investigate their growth performance and stress response. Fish were fed a commercial diet to apparent satiation for 8 weeks of the experiment. Significantly higher weight gain and specific growth rates (SGR) were observed in fish exposed to a 24L:0D photoperiod followed by 16L:8D, 6L:6D and 12L:12D photoperiods ( P < 0.05). Food intake was significantly higher in fish exposed to 24L:0D followed by 6L:6D, 16L:8D and 12L:12D photoperiods ( P < 0.05). There was no significant difference in the feed conversion efficiency (FCE) between fish exposed to 24L:0D and 16L:8D photoperiods, but the FCE in both photoperiods was significantly higher than that of 6L:6D and 12L:12D photoperiods. There were no significant differences in hematocrit levels and plasma levels of cortisol and glucose among the treatments at the end of experiment. The results demonstrated that the growth performance of juvenile red sea bream reared from 1 to 30 g can be stimulated significantly by using a continuous (24L:0D) photoperiod without any measurable significant stress response in fish.

Alleviating Heat Loss Associated with Modified Ultrafiltration

Modified ultrafiltration (MUF) has been described and utilized for the removal of extracellular water in the immediate postcardiopulmonary bypass (CPB) period. This technique has been associated with improved hematological status and hemodynamic stability post cardiopulmonary bypass. Hypothermia during the MUF period has been described as a complication associated with this technique. Decreased patient temperature may be associated with increased bleeding causing an increase in time to sternal re-approximation, OR time, decreases in cardiac function, peripheral vascular perfusion, and an increase in blood product utilization. These complications may reduce some of the benefits described with the use of MUF. The purpose of this study was to evaluate the use of a heated MUF infusion line to reduce the heat loss associated with this technique in a pediatric population. After obtaining Committee for Protection of Human Subjects exemption, a retrospective review to evaluate the efficiency of the hot MUF infusion line was undertaken. Twenty patients under 10 kg who underwent MUF before the change to a heated infusion line were retrospectively identified and matched to patients undergoing MUF with a heated infusion line with regard to weight, lesion, procedure, surgical staff and technique, and disposable equipment. Groups were evaluated for temperature and hematocrit change during the MUF period, blood loss and transfusion postprotamine in the OR and 24 h, and time to sternal re-approximation postprotamine. Statistical significance was seen between the two groups in temperature (−0.24 ± 0.72 vs. − 1.58 ± 0.89°C; p &lt; .0001) with the HotLine group having little change post MUF. Significance was also seen in the last OR temperature recorded (37.0 ± 1.2 vs. 36.0 ± 1.0°C; p = .01) with the HotLine group having the higher temperature. There were no significant differences in hematocrit levels at 24 hours, last in the OR, or the change after the MUF period. No significant difference was found in blood transfused postprotamine in the OR, 24-h blood transfused, 24-h chest tube loss, or sternal closure. The study suggests that the use of a heated MUF infusion line safely reduces the heat loss associated with MUF in the immediate post-operative period.