Diethylenetriamine Pentaacetate Research Articles

Biodegradable Citrate‐Based Polymers Enable 5D Monitoring of Implant Evolution

AbstractBiodegradable tissue engineering scaffolds have garnered increasing interest for their role in providing mechanical support, promoting tissue regeneration, and eliminating the need for removal. However, the in vivo degradation processes remain challenging to track. Here, a novel biodegradable polymer, N‐methyldiethanolamine (MDEA) and Gadolinium(III) diethylenetriamine pentaacetate (Gd‐DTPA) modified biodegradable photoluminescent polymers (BPLPMGd), which combines near‐infrared (NIR) fluorescence and magnetic resonance (MR) dual‐modality imaging are introduced to monitor scaffold degradation in vivo. The chemical structure of BPLPMGd is characterized and its dual‐imaging properties in vitro are evaluated. Subsequently, non‐invasive dual‐modality imaging to track the degradation of implanted BPLPMGd scaffolds is performed in a rat model, comparing these results with histological data. This approach reveals that BPLPMGd enables reliable non‐invasive tracking of the degradation, where NIR fluorescence imaging offers a qualitative and quantitative analysis of scaffold mass loss, total volume and solid content changes, while magnetic resonance imaging (MRI) details structural and morphological changes, allowing for 5D monitoring of implant degradation, including 3D structure, location, mass, volume, and geometry. The combination of these imaging modalities provides a comprehensive view of scaffold degradation, where the synergistic use of both yields results greater than either modality alone, offering unprecedented 5D information of implantable devices. This innovative approach has potential applications in regenerative engineering and beyond.

Transforming Zinc-Ion Batteries with DTPA-Na: A Synergistic SEI and CEI Engineering Approach for Exceptional Cycling Stability and Self-Discharge Inhibition.

Aqueous zinc-ion batteries (ZIBs) hold immense potential for large-scale energy storage, but their practical implementation faces significant challenges related to the zinc anode, including dendrite formation, corrosion, and hydrogen evolution. This study addresses these challenges by introducing diethylenetriamine pentaacetate sodium salt (DTPA-Na) as a novel electrolyte additive. DTPA-Na exhibits a unique dual functionality, enabling the formation of a robust, multi-layered solid electrolyte interphase (SEI) on the zinc anode and a stable cathode electrolyte interphase (CEI) on the MnOOH cathode. The engineered SEI effectively suppresses interfacial side reactions, facilitates uniform zinc deposition, and mitigates dendrite growth, while the CEI inhibits MnOOH dissolution and detrimental cathode side reactions. This synergistic SEI/CEI engineering approach significantly enhances ZIB performance, achieving remarkable cycling stability and self-discharge inhibition, as evidenced by the extended lifespan of Zn||Zn symmetrical cells (4400 hours at 0.5 mA/cm2) and the exceptional capacity retention of Zn||MnOOH full cells after prolonged rest (98.61 % after 720 hours).

Influence of various ion chelators on mechanical, transport and microstructure properties of cement-based materials

Influence mechanism of various ion chelators on the mechanical, transport, and microstructure properties of cement-based materials was explored. The ion chelators included pentasodium diethylenetriamine pentaacetate, tetrasodium ethylenediaminetetraacetate, sodium hexametaphosphate and tetrasodium glutamate diacetate, with a dosage of 1% of the cement mass. The effect of ion chelators on the mechanical, transport, and pore structure properties was evaluated by compressive, sorptivity, and mercury intrusion porosimetry (MIP) tests. The composition and microstructure of hydration products were studied by scanning electron microscope (SEM) and X-ray diffractometer (XRD). Test results indicated that four ion chelators reduced the mechanical and transport properties, and increased the porosity of specimens at 3 d and 7 d. And four ion chelators improved the mechanical and transport properties, and reduced porosity at 28 d and 56 d. The fundamental reason for the differences in the basic properties of cement-based materials caused by the types of ion chelators is that different ion chelators possess various calcium chelating capacities and stability of calcium complexes, which leads to different impact on the complexation substitution reaction process. Moreover, compared to the stability of calcium complexes, the influence of calcium chelating ability on the basic properties of specimens is more significant in the early curing ages.

The Impact of Organic and Chemical Organic Fertilizers on the Efficiency of Cadmium Mobility Reduction by Potassium Hydroxide Modified Biochar

The aim of this study was to investigate the impact of organic and chemical organic fertilizers on the translocation of cadmium in crude oil-contaminated soil treated with potassium hydroxide-modified biochar (KOH-biochar). The soil sample was collected from Chonburi Province. The soil was characterized as moderately acidic sandy loam with relatively low organic matter, medium salinity, medium cation exchange capacity, and high nutrient levels. The concentration of cadmium in the soil fell within the acceptable range for agricultural use. The KOH-biochar exhibited strong alkalinity, a high carbon/nitrogen (C/N) ratio, and an oxygen/carbon (O/C) ratio. Crude oil was slightly acidic, with high organic matter content and low sulfur and cadmium concentrations. The synthetic soil created in this study composed of 5% crude oil, and 100 mg/kg of cadmium. KOH-biochar, organic and chemical organic fertilizers were applied to this synthetic soil. Subsequently, the soil was subjected to extraction with 0.005 M diethylenetriamine pentaacetate (DTPA), and a sequential extraction method was employed to determine six different forms of cadmium in the soil samples. Cadmium concentrations in the extracts were measured using a graphite furnace atomic absorption spectrophotometer. The findings revealed that fertilizers effectively slowed down the movement of cadmium. Fertilizer application led to the transformation of cadmium from unstable forms to more stable forms within the soil. Fertilizer with the highest organic matter content and pH showed the least cadmium mobility. Increased nitrogen and phosphorus content in the fertilizer resulted in slightly higher cadmium mobility within the soil. Conversely, higher potassium content in the fertilizer led to slightly reduced cadmium mobility in the soil.

Transforming Zinc‐Ion Batteries with DTPA‐Na: A Synergistic SEI and CEI Engineering Approach for Exceptional Cycling Stability and Self‐Discharge Inhibition

Aqueous zinc‐ion batteries (ZIBs) hold immense promise for large‐scale energy storage, but their practical application is hindered by zinc anode limitations. We introduce diethylenetriamine pentaacetate sodium salt (DTPA‐Na) as a novel electrolyte additive to address these challenges. DTPA‐Na's unique dual functionality enables the formation of robust, multi‐layered solid electrolyte interphases (SEI) on the zinc anode and stable cathode electrolyte interphases (CEI) on the MnOOH cathode. This synergistic SEI/CEI engineering approach effectively suppresses interfacial side reactions, promotes uniform zinc deposition, and inhibits dendrite growth, leading to exceptional cycling stability and self‐discharge inhibition. Asymmetrical cells employing DTPA‐Na achieve an unprecedented 32,000 cycles at a high charging rate of 50 mA/cm2, while symmetric cells demonstrate a lifespan of 160 hours with 95% zinc utilization. Full Zn||MnOOH cells exhibit remarkable stability, maintaining 98.61% capacity retention after 720 hours of self‐discharge and negligible capacity decay over 5000 cycles. Our work highlights the transformative potential of DTPA‐Na as a dual‐functional electrolyte additive, paving the way for high‐performance ZIBs for practical energy storage applications.

Enhanced thorium decorporation and mitigation of toxicity through combined use of Liv52® and diethylenetriamine pentaacetate

Thorium-232 (Th-232) is a promising fuel for advanced nuclear reactors. However, in case of internal human exposure to Th, there is currently no effective modality for its removal from liver and skeleton or for mitigating its effect. The FDA-approved agent, diethylenetriaminepentaacetate (DTPA), can remove Th and other actinides from blood circulation only. For the first time, a rationally-selected polyherbal hepatoprotective i.e. Liv52® (L52S), was evaluated in-combination with DTPA for its Th decorporation ability in Swiss mice. Inductively-coupled plasma mass spectroscopic analysis showed that oral administration of L52S in conjunction with DTPA significantly decreased Th burden from liver (20 %) and skeleton (33 %) as well as enhanced Th excretion (∼2.5 folds) through urine in comparison to DTPA or L52S alone. The combinatorial therapy was found to be complementary in-action, ameliorating Th-induced tissue damage in liver, spleen, and bone more effectively than monotherapy. Furthermore, markers of liver function (alanine transaminase) and liver inflammation and fibrosis (NF-κB & keratin) further validated the beneficial effect of L52S. The human consumption of L52S for various liver disorders further supports its clinical application for Th decorporation and mitigation of its health effects.

Natural Products of Licorice for Uranium Decorporation with Low Toxicity and High Efficiency.

The development and exploration of uranium decorporation agents with straightforward synthesis, high removal ability, and low toxicity are crucial guarantees for the safety of workers in the nuclear industry and the public. Herein, we report the use of traditional Chinese medicine licorice for uranium decorporation. Licorice has good adsorption performance and excellent selectivity for uranium in the simulated human environment. Glycyrrhizic acid (GL) has a high affinity for uranium (p(UO2) = 13.67) and will complex with uranium at the carbonyl site. Both licorice and GL exhibit lower cytotoxicity compared to the commercial clinical decorporation agent diethylenetriamine pentaacetate sodium salts (CaNa3-DTPA). Notably, at the cellular level, the uranium removal efficiency of GL is eight times higher than that of CaNa3-DTPA. Administration of GL by prophylactic intraperitoneal injection demonstrates that its uranium removal efficiency from kidneys and bones is 55.2 and 23.9%, while CaNa3-DTPA shows an insignificant effect. The density functional theory calculation of the bonding energy between GL and uranium demonstrates that GL exhibits a higher binding affinity (-2.01 vs -1.15 eV) to uranium compared to DTPA. These findings support the potential of licorice and its active ingredient, GL, as promising candidates for uranium decorporation agents.

Preoperative Super-Selective Embolization versus "On-Clamp" Laparoscopic Partial Nephrectomy for T1 Renal Tumors- A Prospective Randomized Study.

To analyze and compare the intraoperative and post-operative outcomes of "on-clamp" laparoscopic partial nephrectomy (LPN) with "preoperative super-selective angioembolization" before LPN. This randomized clinical study was conducted at Gauhati Medical College Hospital, Guwahati, India, between November 2021 and November 2023. Adult patients of either gender diagnosed with T1 renal tumors were included in the study. All patients underwent diethylenetriamine pentaacetate scan preoperatively and at 1-month follow-up. The patients were randomized using a parallel group design with an allocation ratio of 1:1 to receive either preoperative angioembolization followed by LPN or conventional "on-clamp" LPN. Demographic and baseline parameters were recorded along with pre- and post-operative data. There was no significant difference between the two groups in terms of age (P = 0.11), gender distribution (P = 0.32), body mass index (P = 0.43), preoperative hemoglobin (P = 0.34), and preoperative estimated glomerular filtration rate (eGFR; P = 0.64). One patient in the embolization group required radical nephrectomy because of accidental backflow of glue into the renal artery during embolization whereas four patients required clamping due to inadequate embolization. Preoperative super-selective embolization yielded significantly less blood loss, compared to "on-clamp" LPN (145 [50.76 mL] vs. 261 [66.12 mL], P < 0.01). There was no significant difference between post-operative eGFR (at 1 month) between the two groups (P = 0.71). Preoperative embolization offers improved outcomes in the dissection plane, total operative time, and blood loss, compared to conventional "on-clamp" LPN but has no significant effect on change in eGFR.

Radiolabeling and preclinical animal model evaluation of DTPA coupled 99mTc-labelled flutamide complex ([99mTc]DTPA-FLUT) as a potential radiotracer for cancer imaging.

Advances in molecular imaging strategies have had an effect on precise diagnosis and treatment. Research has been intensified to develop more effective and versatile radiopharmaceuticals to uplift diagnostic efficiency and, consequently, the treatment. To label the flutamide (FLUT) coupled with diethylenetriamine pentaacetate (DTPA) with technetium-99 m (99mTc) and to evaluate its binding efficiency with rhabdomyosarcoma (RMS) cancer cells. Radiolabeling of FLUT with 185 MBq freshly eluted 99mTcO4-1 was carried out via DTPA bifunctional chelating agent using stannous chloride reducing agent at pH 5. The labeled compound was assessed for its purity using chromatography analysis, stability in saline and blood serum, AND charge using paper electrophoresis. Normal biodistribution was studied using a mouse model, while binding affinity with RMS cancer cells was studied using an internalization assay. The in vivo accumulation of RMS cancer cells in a rabbit model was monitored using a SPECT gamma camera. Radiolabeling reaction displayed a pharmaceutical yield of 97% and a stability assay showed >95% intact radiopharmaceutical up to 6 h in saline and blood serum. In vitro internalization studies showed the potential of [99mTc]DTPA-FLUT to enter into cancer cells. This biodistribution study showed rapid blood clearance and minimum uptake by body organs, and scintigraphy displayed the [99mTc]DTPA-FLUT uptake by lesion, induced by RMS cancer cell lines in rabbit. Stable, newly developed [99mTc]DTPA-FLUT seeks its way to internalize into RMS cancer cells, indicating it could be a potential candidate for the diagnosis of RMS cancer.

The Influence of Chemical Fertilizers on the Effectiveness of Biochar in Mitigating Cadmium Mobility in Soil

The focus of this study was to explore how various fertilizers influence the movement of cadmium in soil treated with biochar. The research utilized a strong acid sandy loam soil from Chanthaburi Province, naturally rich in organic matter with moderate cation exchange capacity and low nitrogen, potassium, phosphorus, sulfate, salinity and chloride levels. The soil was purposely contaminated with 50 mg kg-1 of cadmium and treated with biochar derived from water hyacinth through pyrolysis at 450°C for an hour. This biochar displayed moderate alkalinity, high organic matter, phosphorus, potassium, and cation exchange capacity, but low nitrogen content. Analytical techniques like Scanning Electron Microscopy and Fourier-Transform Infrared Spectroscopy were employed to study the surface characteristics of the biochar. The cadmium adding soil was blended with 10% biochar and various chemical fertilizers at a 0.04% ratio. Diethylenetriamine pentaacetate (DTPA) extraction was used to assess the bioavailability of cadmium to plants in the soil, while sequential extraction was conducted to identify the different forms of cadmium present in the soil. The study revealed distinct effects of different fertilizers on cadmium mobility. Fertilizers like 46-0-0 and 0-3-0 caused a transformation of cadmium from stable to less stable forms, increasing the bioavailability of cadmium to plants. Conversely, fertilizers such as 15-15-15, 0-0-50, 0-0-60, and 0-52-34 shifted cadmium from less stable to more stable forms, resulting in decreased cadmium extracted by DTPA. Other fertilizers showed no significant impact on cadmium mobility in the soil. A stepwise linear regression analysis highlighted that nitrogen content, potassium content, and electrical conductivity were influential factors affecting cadmium mobility.

Efficacy of Endopyelotomy for Secondary Pelvi-Ureteric Junction (PUJ) Obstruction in Terms of Improvement in Renal Function.

Objective The objective of this study is to measure renal functionimprovement after endopyelotomy for secondary pelvi-ureteric junction (PUJ) obstruction using technetium-99m diethylene-triamine-pentaacetate (DTPA) renal scintigraphy. Material and methods This descriptive study was carried out at the Department of Urology, Institute of Kidney Diseases, Peshawar, Pakistan from June 1, 2021, to May 31, 2023. The study included 118 secondary PUJ blockage patients who underwent endopyelotomy. Patient demographics, clinical history, and preoperative imaging findings were obtained. DTPA renal scintigraphy assessed renal function improvement postoperatively at intervals to determine the efficacy of endopyelotomy. Results The majority of the patients included in the study were male (n=65, 55.1%). The average age of the patients was 45.2 years, with the majority falling within the age range of 46-60 years (n=42, 35.6%). All patients had ultrasonography and computed tomography imaging done, and preoperativerenal functions were obtained. Comorbidities included hypertension in 32 (27.12%) and diabetes in 18 (15.25%). DTPA renal scintigraphy showedimproved renal function in 81.35% of patients at three months, 88.13% at six months, and 83.05% at 12 months; 15.3% of patients needed further treatments, and 5.1% had PUJ blockage recurrence. Conclusion This study offers significant insights into the results and complexities of endopyelotomy in patients suffering from PUJ blockage. The findings indicate that the technique efficiently enhances kidney function and alleviates symptoms in most patients. However, the study also emphasizes the need to monitor patients undergoing this procedure.

A Single-Center Study on the Clinical Profiles and Ultrasonographic Assessments of Living Kidney Donors in the Marathwada Region of Maharashtra.

Living donor kidney transplantation plays a vital role in renal replacement therapy, particularly in India, where a substantial increase in kidney transplants has been observed. Thorough assessments of living kidney donors are crucial, focusing on parameters such as kidney size and glomerular filtration rate (GFR). Despite the importance of GFR in donor assessments, there is a noticeable lack of data on normal GFR ranges in the Indian population. This study aims to address the gap in knowledge by establishing a reference range for GFR in healthy kidney donors from the Marathwada region of Maharashtra. The research also explores the clinical profiles and ultrasonographic features of living kidney donors. A retrospective analysis was conducted at the Mahatma Gandhi Mission (MGM) Medical College and Hospital in Aurangabad, involving 134 living kidney donors. Inclusion criteria encompassed healthy donors with a BMI of less than 30 kg/m², while donors with uncontrolled hypertension, diabetes, microalbuminuria, or a measured GFR below 70 mL/min/1.73 m² were excluded. Comprehensive medical histories, demographic parameters, and ultrasonographic assessments were conducted, with GFR measured using 99M technetium diethylenetriamine pentaacetate scans. The study reveals that the majority of donors were females (80.6%), and the highest number fell within the 41-50 age group. Parents constituted the primary donor category (68.7%), reflecting a familial inclination toward organ donation. Ultrasonographic assessments indicated larger kidney sizes compared to other studies, suggesting regional or population-specific differences. The mean GFR for the right and left kidneys, as well as the total GFR, was within the expected range. The negative correlation between age and GFR emphasizes the need to consider age in donor assessments. The findings emphasize the unique features of this population, including a higher average age, female preponderance, and larger kidney sizes. The study contributes to the understanding of living kidney donors' profiles in the region and highlights the importance of individualized assessments in the donor selection process.

Synthesis, characterization and evaluation of novel manganese nanoclay polymer composite and nano‐MnO2 in wheat

AbstractBackgroundManganese (Mn) deficiency due to nutrient mining by high yielding cereal–cereal cropping patterns and forgetfulness of Mn fertilizer applications becomes potential challenge in crop production.AimNano‐enabled Mn fertilizers can be safer and more nutrient efficient than conventional Mn fertilizers (nutrient use efficiency ≈ 1%–3%). However, studies about nano‐Mn fertilizer synthesis and their behaviour in soil–plant system are rare.MethodsIn this study, two novel nano‐Mn fertilizers, that is nano‐MnO2 (NMO) and manganese nanoclay polymer composites (Mn‐NCPC), were synthesized, characterized (dynamic light scattering, X‐ray diffraction, Scanning electron microscopic and energy‐dispersive X‐ray, Fourier transform infrared spectroscopy etc.) and investigated for their impact on growth, yield and nutrient acquisition by wheat crop (Triticum aestivum L., variety HD‐2967) in a pot culture experiment. Treatment comprised 25%, 50% and 100% of recommended dose of Mn (RDMn) through NMO along with 100% RDMn through MnSO4·H2O (MS). Effect of exposure route was also investigated using foliar spray of NMO at tillering stage. Mn‐NCPC was found to be most efficient Mn fertilizer in terms of yield, Mn uptake and use efficiency by wheat crop.ResultsNano‐sized formulations improved the solubility of Mn in soil due to its higher active surface area (NMO) and slow‐release behaviour (Mn‐NCPC); thus, minimal losses happened due to the fixing of Mn in oxide/hydroxide forms. Application of 25% RDMn through NMO fertilizers maintained equitant diethylenetriamine pentaacetate Mn content to 100% RDMn through MnSO4·H2O. Mn‐NCPC stimulated the soil enzymatic activities, namely dehydrogenase, acid–alkaline phosphatase activities. Mn‐NCPC and NMO at 100% RDMn recorded 3.51% and 5.20% improvement in grain yield, respectively, when compared to MnSO4·H2O 100%.ConclusionsMn fertilizer doses can be reduced up to 25% of RDMn when applied through NMO or Mn‐NCPC fertilizers. However, effects of Mn‐NCPC and NMO need to be critically evaluated in long‐term field experiments in various cropping systems especially under cereal–cereal sequences for economic profitability and wide‐scale farmer's adaptability.

A Novel Method Using Gadolinium-Ethoxybenzyl Diethylenetriamine Pentaacetate Acid-Enhanced Magnetic Resonance Imaging for Predicting Post-Hepatectomy Liver Failure in Hepatocellular Carcinoma Patients with a Major Portal Vein Tumor Thrombus

Introduction: The usefulness of gadolinium-ethoxybenzyl diethylenetriamine pentaacetate acid-enhanced magnetic resonance imaging (EOB-MRI) in assessing the functional future remnant liver volume (fFRLV) to predict post-hepatectomy liver failure (PHLF) has been previously reported. Herein, we evaluated the efficacy of this technique in patients with hepatocellular carcinoma (HCC) with a major portal vein tumor thrombus (PVTT). Methods: This study included 21 patients with PVTT in the ipsilateral first-order branch (Vp3) and 30 patients with PVTT in the main trunk/contralateral branch (Vp4). To evaluate fFRLV, the signal intensity (SI) of the remnant liver was determined on T1-weighted images, using both conventional and newly developed methods. The fFRLV was calculated using the SI of the remnant liver and muscle, remnant liver volume, and body surface area. Preoperative factors predicting PHLF (≥grade B) in HCC patients with Vp3/4 PVTT were evaluated. Results: In the Vp3 group, we found fFRLV area under the receiver-operating characteristic curves (AUCs) above 0.70 (AUC = 0.875, 0.750) using EOB-MRI results calculated using either the plot or whole method. None of the parameters in the Vp4 group had an AUC greater than 0.70. Conclusion: The fFRLV calculated by EOB-MRI using the whole method can be as useful as the conventional method in predicting PHLF (≥grade B) for HCC patients with Vp3 PVTT.

Enhancing oral bioavailability of Ca-DTPA by self double emulsifying drug delivery system (SDEDDS)

Objective BCS class III drug (highly soluble, poorly permeable) possesses low oral bioavailability. The research work highlights the utility of self-double emulsifying drug delivery system (SDEDDS) which are stable isotropic mixture of w/o primary emulsion and hydrophilic surfactants for improving oral bioavailability of Ca-DTPA (Calcium diethylenetriamine pentaacetate). Upon oral administration, SDEDDS rapidly emulsifies into w/o/w double emulsions in the aqueous gastrointestinal environment, with hydrophilic drugs entrapped inside oil reservoirs. Methods SDEDDS formulation was successfully developed using excipients, that is, medium chain triglycerides, oleic acid, phospholipids, Span 80, Tween 80 using double emulsification technique. Results The optimized formulation F4 (Aq. phase: 11.6%w,w; MCT & oleic acid: 70.9%w/w; Span 80:17.5%w/w; Lecithin:16%w/w and Tween 80 (10%w/w)) appeared bright yellow liquid which upon dilution appeared milky white within 2 min, droplet size (501.7 nm), pdi value (0.044), zeta potential (-52 mV), entrapment efficiency (79.6 ± 1.63), viscosity (72.2 ± 1.8 mpA.s), significant high cumulative in vitro drug permeation (CDP) and 2.17-fold increase in apparent permeability coefficient. Pharmacokinetic studies in rats showed 1.17-fold increases in AUC of F4 and comparatively higher plasma levels (Cmax) compared with pure drug administered orally. The Absolute (OF4, OD) and Relative bioavailability was found to be 14.52%, 12.35%, and 117.47%, respectively. Conclusion The present studies have clearly demonstrated that SDEDDS could readily form w/o/w double emulsions in vivo with enhanced in vitro and in vivo oral bioavailability. Therefore, considerable augmentation in the rate and extent of oral drug absorption ratified the better performance of the SDEDDS in enhancing the bioavailability of Ca-DTPA.

Enhancing microbial superoxide generation and conversion to hydroxyl radicals for enhanced bioremediation using iron-binding ligands

Harnessing bacteria for superoxide production in bioremediation holds immense promise, yet its practical application is hindered by slow production rates and the relatively weak redox potential of superoxide. This study delves into a cost-effective approach to amplify superoxide production using an Arthrobacter strain, a prevalent soil bacterial genus. Our research reveals that introducing a carbon source along with specific iron-binding ligands, including deferoxamine (DFO), diethylenetriamine pentaacetate (DTPA), citrate, and oxalate, robustly augments microbial superoxide generation. Moreover, our findings suggest that these iron-binding ligands play a pivotal role in converting superoxide into hydroxyl radicals by modulating the electron transfer rate between Fe(III)/Fe(II) and superoxide. Remarkably, among the tested ligands, only DTPA emerges as a potent promoter of this conversion process when complexed with Fe(III). We identify an optimal Fe(III) to DTPA ratio of approximately 1:1 for enhancing hydroxyl radical production within the Arthrobacter culture. This research underscores the efficacy of simultaneously introducing carbon sources and DTPA in facilitating superoxide production and its subsequent conversion to hydroxyl radicals, significantly elevating bioremediation performance. Furthermore, our study reveals that DTPA augments superoxide production in cultures of diverse soils, with various soil microorganisms beyond Arthrobacter identified as contributors to superoxide generation. This emphasizes the universal applicability of DTPA across multiple bacterial genera. In conclusion, our study introduces a promising methodology for enhancing microbial superoxide production and its conversion into hydroxyl radicals. These findings hold substantial implications for the deployment of microbial reactive oxygen species in bioremediation, offering innovative solutions for addressing environmental contamination challenges.

Investigation of Chelating Agents for the Removal of Thorium from Human Teeth upon Nuclear Contamination.

Thorium-232 (232Th) is a radioactive heavy metal that is of increasing interest as a source of nuclear energy. However, upon nuclear incidents, the ingestion or inhalation of Th in major quantities can contribute to chemical and radiological health problems, including accumulation in the bone tissue and an increased risk of developing pancreatic, lung, and hematopoietic cancers. The major mineral component of the bone is hydroxyapatite (HAP)─also the major mineral component of the teeth. As such, the teeth are the first site of exposure upon oral ingestion of Th-contaminated materials, and Th can pose a potential risk to teeth development. In essence, in the case of human contamination, it is critical to identify effective chelating agents capable of removing Th. Using a batch study methodology, this present work investigates the uptake and the removal of Th from synthetic HAP and from teeth samples by diethylenetriamine pentaacetate (DTPA), ethylenediaminetetraacetic acid (EDTA), and other promising chelating agents. Th uptake over synthetic HAP exceeds 98% at physiological pH with <1 min of contact time and uptake exceeds 90% across the entire pH range. Regarding teeth, over 1 mg Th uptaken per gram of tooth is observed after 24 h. The overall effectiveness of chelating agents for the removal of Th from is as follows: DTPA > EDTA > NaF/mouthwash/3,4,3-LI(1,2-HOPO); this trend was observed both in synthetic HAP and Th-impregnated teeth samples.

Predictive Value of Camera-based Donor Glomerular Filtration Rate Estimation on the Immediate Renal Allograft Outcome Following Live-related Renal Transplant: A Single-center Retrospective Study.

The purpose of this study was to assess the association of measured glomerular filtration rate (mGFR) using camera-based method with early transplant outcomes. Diethylenetriamine pentaacetate renograms of all voluntary kidney donors between January 2016 and December 2022 at Kasturba Hospital, Manipal, India, were retrieved for the study. Recipients' posttransplant biochemical parameters were collected and compared against donors with scaled mGFR >80 ml/min/1.73 m2 (Group 1) and with mGFR between 60 and 80 ml/min/1.73 m2 (Group 2). Donor-recipient pair age, anthropometric parameters, and their differences were also assessed against the immediate transplant outcome. Posttransplant immediate graft function was assessed by posttransplant nadir serum creatinine, day to achieve nadir serum creatinine, the incidence of slow graft or delayed graft function, and serum creatinine at 1-month posttransplantation. Recipients with serum creatinine of >2.5 mg/dl on posttransplant day 7 were taken as slow graft function. A total of 161 donor-recipient pairs were analyzed in the study. In recipients who showed persistently high serum creatinine posttransplant, older donor age(p < 0.001), higher difference in body mass index among the donor-recipient pair (p= 0.03), and mGFR <80ml/min (p < 0.001) were significantly associated. Slow graft function was significantly more in Group II recipients, with donors having mGFR <80ml/min as compared to Group I with mGFR >80 ml/min (37.3% vs. 10.6%) (P < 0.001). Camera-based mGFR using Gates' formula is a reliable tool to predict inferior graft outcomes in the immediate posttransplant period. Kidneys from donors with mGFR of 60-80 mL/min/1.73 m2 are likely to experience slow graft function in the immediate posttransplant period.

A magnetically recyclable carboxyl-functionalized chitosan composite for efficiently removing methyl orange from wastewater: Isotherm, kinetics, thermodynamic, and adsorption mechanism

In this study, a kind of magnetically recyclable adsorbent for dyes was synthesized by grafting diethylenetriamine pentaacetate acid (DTPA) to the composite of Fe3O4 microspheres and crosslinked chitosan (CS). The microstructures, molecular structure, crystal structure, and magnetic hysteresis loops of the chitosan matrix adsorbent before and after grafting was characterized. The results suggested that DTPA was covalent bonded with the composite of Fe3O4 microspheres and chitosan. The modified composite has larger specific surface area and can realize rapid solid-liquid separation. Batch experiments were conducted to optimize the parameters affecting the adsorption of methyl orange (MO). The adsorption process could be better described by pseudo-second-order kinetics model and Langmuir isotherm equation, and its saturated adsorption capacity of the modified adsorbents was 1541.5 mg·g−1 at 25 °C, which was 1.40 times of that the unmodified adsorbent (1104.1 mg·g−1). The obtained values of the thermodynamic parameters indicated that the adsorption was a spontaneous process. The regeneration experiment proved the stability and reproducibility of the adsorbent even after five cycles of adsorption-desorption. The primary adsorption mechanism was electrostatic interaction and hydrogen bonding. The adsorbent could be potentially applied for removing dyes from wastewater in wide pH of range, especially acid wastewater.

Prediction tool for renal adaptation after living kidney donation using interpretable machine learning.

Post-donation renal outcomes are a crucial issue for living kidney donors considering young donors' high life expectancy and elderly donors' comorbidities that affect kidney function. We developed a prediction model for renal adaptation after living kidney donation using interpretable machine learning. The study included 823 living kidney donors who underwent nephrectomy in 2009-2020. AutoScore, a machine learning-based score generator, was used to develop a prediction model. Fair and good renal adaptation were defined as post-donation estimated glomerular filtration rate (eGFR) of ≥ 60 mL/min/1.73 m2 and ≥ 65% of the pre-donation values, respectively. The mean age was 45.2 years; 51.6% were female. The model included pre-donation demographic and laboratory variables, GFR measured by diethylenetriamine pentaacetate scan, and computed tomography kidney volume/body weight of both kidneys and the remaining kidney. The areas under the receiver operating characteristic curve were 0.846 (95% confidence interval, 0.762-0.930) and 0.626 (0.541-0.712), while the areas under the precision-recall curve were 0.965 (0.944-0.978) and 0.709 (0.647-0.788) for fair and good renal adaptation, respectively. An interactive clinical decision support system was developed. The prediction tool for post-donation renal adaptation showed good predictive capability and may help clinical decisions through an easy-to-use web-based application.