Dietary Yeast Supplementation Research Articles

Dietary supplementation of prebiotic yeast Saccharomyces cerevisiae cell wall promotes growth performance and intestinal health in broiler chickens challenged with Clostridium perfringens

ABSTRACT 1. This study evaluated the effectiveness of yeast (S accharomyces cerevisiae) cell wall (YCW) supplementation on the growth performance, carcase characteristics, serum biomarkers, liver function, ileal histology and microbiota of broiler chickens challenged with Clostridium perfringens (C. perfringens). 2. In a 35-d trial, 240 chicks aged 1-d-old were randomly assigned to one of four treatment groups, each with 10 replicates: control (CON) with no challenge or additives, challenged with C. perfringens (CHAL), CHAL and supplemented with YCW at either 0.25 g/kg (YCW0.25) or 0.5 g/kg (YCW0.5). 3. In comparison to CON, the CHAL birds had reduced growth performance, survival rate, dressing percentage, breast meat yield, levels of total protein (TP), globulin (GLO), glucose (GLU), total antioxidant capacity (T-AOC) and total superoxide dismutase (T-SOD), as well as a decreased Lactobacillus population (P < 0.01). Additionally, this group showed elevated levels of glutamic oxaloacetic transaminase (GOT), glutamic pyruvic transaminase (GPT), and C. perfringens count (P < 0.01). Compared to CHAL, the YCW0.25 or YCW0.5 groups had improved growth performance, survival rate, dressing percentage, breast meat yield, levels of TP, GLO, GLU, and T-AOC, as well as the activities of T-SOD, GOT, and GPT, villus height, villus surface area, villus height to crypt depth ratio, and the populations of both Lactobacillus and C. perfringens; (P < 0.01). 4. The data suggested that YCW supplementation at either 0.25 or 0.50 g/kg can restore the growth performance of broiler chickens during a C. perfringens challenge.

Effects of Dietary Selenium Yeast Supplementation on Lactation Performance, Antioxidant Status, and Immune Responses in Lactating Donkeys.

Donkey milk is a traditional medicinal food with various biological activities. However, its production is very low, and lactating donkeys often experience oxidative stress, leading to a further decline in milk yield. In this study, we supplemented the diets of lactating donkeys with yeast selenium (SY) to investigate its effects on lactation performance, antioxidant status, and immune responses, and we expected to determine the optimum additive level of SY in the diet. For this study, 28 healthy lactating Dezhou donkeys with days in milk (DIM, 39.93 ± 7.02 d), estimated milk yield (EMY, 3.60 ± 0.84 kg/d), and parity (2.82 ± 0.48) were selected and randomly divided into 4 groups of 7 donkeys in each: Group SY-0 (control), Group SY-0.15, Group SY-0.3, and Group SY-0.5, with selenium supplementation of 0, 0.15, 0.3, and 0.5 mg of Se/kg DM (in form of SY) to the basal diet, respectively. The results showed a dose-dependent increase in milk yield, milk component yield, milk protein production efficiency, milk production efficiency, the activities of glutathione peroxidases (GSH-Px), catalase (CAT), and total antioxidant capacity (T-AOC), as well as the content of serum interleukin-10 (IL-10), white blood cells (WBC), lymphocytes (LYM), red blood cells (RBC), hematocrit, plasma selenium, and milk selenium. Conversely, it presented a dose-dependent decrease in the activity of nitric oxide synthase (iNOS) and the contents of malondialdehyde (MDA), reactive oxygen species (ROS), nitric oxide (NO), interleukin-1β (IL-1β), interleukin-6 (IL-6), and interferon-γ (IFN-γ). In conclusion, the results confirmed that dietary supplementation with SY can improve lactation performance, antioxidant status, and immune responses in lactating donkeys, and the recommended dose of SY was 0.3 mg/kg.

Effects of Dietary Selenium Yeast Supplementation in Pregnant Cashmere Goats on the Development of Offspring Hair Follicles

The objective of this study was to investigate the effects of maternal dietary selenium yeast (SY) supplementation during pregnancy on the hair follicle development of kids. Sixty pregnant Hanshan white cashmere goats were randomly divided into the con group (fed with a basal diet) and the SY group (fed with a basal diet with 0.4 mg/kg SY). SY was supplemented during the pregnancy until the birth of the kids. The growth performance, cashmere performance, hair follicle characteristics, and serum antioxidant capacity of the kids were periodically determined. The results showed that the birth weight of the kids in the SY group was significantly higher than that in the con group (p &lt; 0.05), and the average weight at 15 days, 1 month, 3 months, and 5 months of age increased by 13.60%, 8.77%, 8.86%, and 3.90%, respectively (p &gt; 0.05). The cashmere fineness at early birth was dramatically reduced with SY supplementation (p &lt; 0.001), whereas cashmere length and production were significantly increased at 5 months of age (p &lt; 0.05). Histology assays indicated that the primary hair follicles were fully developed at birth, and there was no significant difference in the number of primary hair follicles between the two groups (p &gt; 0.05). The number of secondary hair follicles and the number and density of active secondary hair follicles in the SY group at 15 days were significantly higher than those in the con group (p &lt; 0.05) and were increased by 11.18%, 6.18%, and 22.55% at 5 months of age, respectively (p &gt; 0.05). The serum antioxidant capacity analysis revealed that the SY group had higher levels of T-AOC, SOD, CAT, and GSH-Px activities and lower levels of MDA (p &gt; 0.05). These results reveal that the maternal dietary supplementation of SY in gestation can promote the morphogenesis and maturation of secondary hair follicles and increase the number and density of secondary hair follicles by enhancing the body’s antioxidant capacity, contributing to the improvement of cashmere quality and yield.

Enhanced Antioxidative Capacity Transfer between Sow and Fetus via the Gut-Placenta Axis with Dietary Selenium Yeast and Glycerol Monolaurate Supplementation during Pregnancy.

This study aims to investigate the impact of dietary supplementation with selenium yeast (SeY) and glycerol monolaurate (GML) on the transfer of antioxidative capacity between the mother and fetus during pregnancy and its underlying mechanisms. A total of 160 sows with similar body weight and parity of 3-6 parity sows were randomly and uniformly allocated to four groups (n = 40) as follows: CON group, SeY group, GML group, and SG (SeY + GML) group. Animal feeding started from the 85th day of gestation and continued to the day of delivery. The supplementation of SeY and GML resulted in increased placental weight and reduced lipopolysaccharide (LPS) levels in sow plasma, placental tissues, and piglet plasma. Furthermore, the redox balance and inflammatory markers exhibited significant improvements in the plasma of sows fed with either SeY or GML, as well as in their offspring. Moreover, the addition of SeY and GML activated the Nrf2 signaling pathway, while downregulating the expression of pro-inflammatory genes and proteins associated with inflammatory pathways (MAPK and NF-κB). Vascular angiogenesis and nutrient transportation (amino acids, fatty acids, and glucose) were upregulated, whereas apoptosis signaling pathways within the placenta were downregulated with the supplementation of SeY and GML. The integrity of the intestinal and placental barriers significantly improved, as indicated by the increased expression of ZO-1, occludin, and claudin-1, along with reduced levels of DLA and DAO with dietary treatment. Moreover, supplementation of SeY and GML increased the abundance of Christensenellaceae_R-7_group, Clostridium_sensus_stricto_1, and Bacteroidota, while decreasing levels of gut microbiota metabolites LPS and trimethylamine N-oxide. Correlation analysis demonstrated a significant negative relationship between plasma LPS levels and placental weight, oxidative stress, and inflammation. In summary, dietary supplementation of SeY and GML enhanced the transfer of antioxidative capacity between maternal-fetal during pregnancy via gut-placenta axis through modulating sow microbiota composition.

Effects of Dietary Selenium Yeast Supplementation on Oxidative Biomarkers of the Brain and Blood in Goats.

The present study evaluated the effects of dietary selenium yeast (SY) on the brain, CSF, and blood of 30 crossbreed goats (5-6months of age) of both sexes. After the acclimatization of 2weeks, they were randomly separated into two groups (n = 15) named C and SY groups. The C group received only a basal diet, while SY received a basal diet along with 0.3mg/kg/diet of SY (Sel-Plex®) in total 0.035mg/kg/diet of SY for 10weeks. Se concentration (µg /g dry weight) in 15 different parts of the goat's brain was accessed, and results showed that the highest concentration was found in the occipital cerebrum (322.0 ± 6.146), whereas the lowest concentration was found in the midbrain (10.33 ± 0.232). Besides, the oxidative biomarkers including GSH (12.13 ± 0.191), GSH-Px (206.7 ± 2.362), GST (23.80 ± 0.279), CAT (14.80 ± 0.279), and SOD (152.5 ± 9.540) were increased in SY as compared to GSH (8.200 ± 0.144), GSH-Px (112.9 ± 1.183), GST (18.93 ± 0.284), CAT (12.53 ± 0.215), and SOD (109.0 ± 1.966) of C. The level of cholesterol was also significantly decreased in the serum of the SY group (84.87 ± 0.960) as compared to C (110.5 ± 0.592). In addition, the cholesterol level in CSF decreased significantly in SY (0.3567 ± 0.016) as compared to C (0.509 ± 0.009). The current research suggests that SY supplementation has improved the brain's antioxidant status, blood biochemistry, and cholesterol levels in both serum and CSF of goats.

Effects of Different Dietary Selenium Sources on the Meat Quality and Antioxidant Capacity of Yellow Catfish (Pelteobagrus fulvidraco).

To assess the effect of dietary selenium (Se) sources on the meat quality and antioxidant capacity of yellow catfish (Pelteobagrus fulvidraco), sodium selenite (Na2SeO3), Se yeast, and selenium-enriched Spirulina platensis (Se-SP) were supplemented in the control diet at 0.30 mg Se/kg feed to formulate four diets. The experimental period lasted 50 days. The results showed that Se levels in the plasma, liver, muscle, and whole body were significantly increased by dietary Se yeast supplementation (P < 0.05) but showed no change in response to Na2SeO3 (P > 0.05). The three types of Se all increased the firmness and decreased the fracturability of the muscles (P < 0.05), but only Na2SeO3 resulted in higher springiness, flexibility, stringiness, and stickiness (P < 0.05). In addition, the muscle n-3 polyunsaturated fatty acid (PUFA) content was increased by Se yeast (P < 0.05). Regarding antioxidant capacity, dietary Se yeast and Se-SP supplementation improved hepatic glutathione peroxidase activity but decreased hepatic malondialdehyde content (P < 0.05). Given these results, Se yeast was found to be the optimal source of Se for yellow catfish for higher tissue retention, antioxidant capacity, and PUFA levels. Dietary Se is an effective way to regulate the meat quality and antioxidant capacity of yellow catfish.

Diet supplemented with Saccharomyces cerevisiae from different fermentation media modulates the faecal microbiota and the intestinal fermentative products in dogs.

This study aimed at evaluating the coefficients of total tract apparent digestibility (CTTAD) of nutrients, metabolisable energy (ME), diet palatability, faecal fermentative products and microbiota of dogs fed yeasts from different fermentation media and its fractions. Four diets were evaluated: control, without yeast (CO); diet with 10 g/kg brewer's yeast (BY); diet with 10 g/kg brewer's yeast + corn yeast (BCY); and diet with 10 g/kg BCY + cell wall fractions (BCYF). Twelve adult dogs were distributed in a randomized block design (periods). Each of the four diets was fed to a group of three dogs per period of 20 days, totalling two periods and six repetitions per treatment. Sixteen adult dogs were used for the palatability test, which compared the CO diet versus each one of the yeast diets. Data with normal distribution were subjected to analysis of variance (p < 0.05). Means were compared by orthogonal contrasts (p < 0.05): (A) CO diet versus BY, BCYand BCYF diets; (B) BY diet versus BCY and BCYF diets; (C) BCY diet versus BCYF diet. There was no difference in the CTTAD and ME of the diets (p > 0.05). Yeast diets reduced faecal odour and indole peak area (p < 0.05). Faecal short-chain fatty acids concentration was greater in dogs fed yeast diets compared to those fed the CO (p < 0.05). Yeast diets showed a higher intake ratio compared to the CO (p < 0.05). The BCY and BCYF diets resulted in a greater abundance of Bacteroides, Faecalibacterium, Coprococcus, and Phascolarctobacterium in relation to the CO (p < 0.05). Our results suggest that dietary yeast supplementation results in beneficial changes in intestinal functionality indicators, mainly with the combination of yeasts from brewers and corn fermentation media. In addition, yeast supplementation improves diet palatability without compromising nutrient digestibility.

The effects of ration particle size and live yeast supplementation on dairy cows performance under heat stress conditions

The objectives of this experiment were to evaluate the effects of ration particle size and dietary supplementation of live yeast (LY; Saccharomyces cerevisiae) on dry matter intake, milk yield and milk quality, apparent nutrient digestibility, ruminal fermentation parameters, and ruminal volatile fatty acids in dairy cattle under heat stress condition. Four multiparous Holstein dairy cattle in midlactation were fed 4 diets: high particle size (HPS), high particle size with 1g/d/cow LY (Levucell® Sc 10 ME Titan® CNCM I-1077 (10 × 109cfu/g) (HPS + LY), short particle size (SPS), short particle size with 1g/d/cow LY (Levucell® Sc 10 ME Titan® CNCM I-1077 (10 × 109cfu/g) (SPS + LY). Treatments were arranged in a 2 × 2 factorial within a 4 × 4 Latin square design. Decreasing ration particle size increased intakes of dry matter (DM), neutral detergent fiber (NDF) (P < 0.05) but decreased peNDF intake (P < 0.0001). Milk production and milk fat percentage were similar in cows. Cows fed SPS had lower milk protein percentage (P < 0.05). No treatments had any significant effect on apparent nutrient digestibility. Ruminal pH was higher in cows supplemented LY (P < 0.05). The ruminal total volatile fatty acid (VFA) concentration and percentage of VFAs were not significantly affected by ration particle size or dietary LY supplementing. Rectal temperature (oC) and respiratory rate (breaths/min) were similar between the groups. In conclusion, it may be concluded that decreasing ration particle size increased DM, and NDF intakes, without affecting milk yield or feed efficiency in cows exposed moderate heat stress.

Dietary Live Yeast Supplementation Influence on Cow’s Milk, Teat and Bedding Microbiota in a Grass-Diet Dairy System

The supplementation of animal feed with microbial additives remains questioning for the traditional or quality label raw milk cheeses with regard to microbial transfer to milk. We evaluated the effect of dietary administration of live yeast on performance and microbiota of raw milk, teat skin, and bedding material of dairy cows. Two balanced groups of cows (21 primiparous 114 ± 24 DIM, 18 multiparous 115 ± 33 DIM) received either a concentrate supplemented with Saccharomyces cerevisiae CNCM I-1077 (1 × 1010 CFU/d) during four months (LY group) or no live yeast (C group). The microbiota in individual milk samples, teat skins, and bedding material were analysed using culture dependent techniques and high-throughput amplicon sequencing. The live yeast supplementation showed a numerical increase on body weight over the experiment and there was a tendency for higher milk yield for LY group. A sequence with 100% identity to that of the live yeast was sporadically found in fungal amplicon datasets of teat skin and bedding material but never detected in milk samples. The bedding material and teat skin from LY group presented a higher abundance of Pichia kudriavzevii reaching 53% (p < 0.05) and 10% (p < 0.05) respectively. A significant proportion of bacterial and fungal ASVs shared between the teat skin and the milk of the corresponding individual was highlighted.

Dietary supplementation of active dry yeast (Saccharomyces cerevisiae) to finishing bulls: effects on growth performance, blood hormones, fatty acid concentrations in the gastrointestinal tract and trace mineral elements utilisation

This study was conducted to investigate the effects of dietary supplementation of active dry yeast (Saccharomyces cerevisiae) on growth performance, circulating ghrelin, fatty acid concentrations in the gastrointestinal tract, and trace mineral elements utilisation of finishing bulls. Twenty Yanbian cattle (bulls) [body weight (BW) 485 ± 38 kg] were randomly divided into two groups (control and treatment groups) and participated in a 100-day finishing trial. The control group (CON) was fed a basal diet (total mixed ration, TMR). The treatment group (active dry yeast, ADY) was fed a basal diet supplemented with ADY (Levucell SC, Saccharomyces cerevisiae CNCM I-1077, viable count ≥8.0 × 109 CFU/g, 1.0 g/bull/day). ADY supplementation significantly increased the dry matter intake (DMI) of bulls (p < .05); significantly increased the concentration of ghrelin O-acyl transferase (GOAT) (p < .05), significantly decreased the concentration of corticotropin-releasing hormone (CRH) (p < .05), tended to increase the concentration of acyl ghrelin (AG) (p = .053) in serum; significantly increased the content of C12:0 in duodenal digesta, the apparent digestibility of Zn, the concentrations Zn and Cu in serum and the deposition of Cu in the liver (p < .05), significantly decreased the deposition of Fe in muscle in finishing bulls (p < .05). In conclusion, ADY supplementation improved DMI of finishing bulls may be related to the change of AG, and a potential mechanism for the change of AG by ADY supplementation is via its effects on the metabolism of C12:0, Zn, and Cu in finishing bulls. HIGHLIGHTS Active dry yeast (ADY) supplementation increased the dry matter intake (DMI) and tended to increase the concentration of acyl ghrelin (AG) in the serum of finishing bulls. ADY supplementation increased the content of C12:0 in duodenal digesta and improved the bioavailability of Zn and Cu of finishing bulls. A potential mechanism for the change of AG by ADY supplementation is via its effects on the metabolism of C12:0, Zn, and Cu in finishing bulls.

Effect of Dietary Supplementation of Chromium and Yeast on in vitro Dry Matter Degradability and Rumen Fermentation Pattern

Background: Yeast (Saccharomyces cerevisiae), widely used probiotics, was found to increase the utilization of fiber and improve the production in ruminants. The concept of chromium (Cr) supplementation has also received a great deal of attention since past decade, as Cr could have supra-physiological benefits for domestic livestock. The present in vitro experiment was conducted to investigate the effect of dietary chromium and yeast supplementation on in-vitro dry matter degradability and rumen fermentation by rumen simulation technique. Methods: The yeast (Saccharomyces cerevisiae) (Y) (5 x 109 CFU /kg) and chromium (Cr) (1.5 mg/kg) supplementation to sorghum stover based complete diets (Basal diet: 10.58% CP) either alone or in combination on in vitro nutrient degradability and rumen fermentation was assessed by rumen simulation technique. Result: Chromium supplementation alone did not affect the cumulative degradability and effective dry matter degradability in vitro compared to BD. Yeast supplementation enhanced (P less than 0.01) in vitro DM degradability on 12, 24 and 48 h, effective DM degradability and lowered (P less than 0.01) ammonia nitrogen level in comparison to control. When compared to control, combination of yeast and Cr positively affected rumen fermentation pattern and in vitro nutrient degradability but no further improvement was observed compared to only yeast supplementation in diet.

Yeast (Saccharomyces cerevisiae) Culture Promotes the Performance of Fattening Sheep by Enhancing Nutrients Digestibility and Rumen Development

Paraprobiotics are potential agents for improving animal health and performance. This experiment investigated the effect of dietary supplementation of yeast (Saccharomyces cerevisiae) culture (YC) on the growth performance, nutrient digestibility, rumen development and microbiome of fattening sheep. Ninety male Hu sheep weighed 38 ± 1.47 kg were randomly assigned to three treatments: CON diet (basal diet), LYC diet (basal diet supplied with 10 and 20 g/d yeast culture at the early and late stages, respectively), and HYC diet (basal diet supplied with 20 and 40 g/d yeast culture at the early and late stages, respectively). Treatments (LYC or HYC) were sprinkled on the feed surface according to the required dosage before feeding the basal diet to each sheep throughout the trial. The trial included early (60 days) and later (30 days) fattening periods. The results showed that average daily gain and feed efficiency were higher (p &lt; 0.05) in the LYC group compared with CON in later and whole stages. Digestibility of DM, OM, CP, NDF and ADF were higher (p &lt; 0.05) in LYC and HYC compared with CON. The retained N, the utilization efficiency of N and the biological value of N were higher (p &lt; 0.05) in LYC compared with CON and HYC. Rumen NH3-N was higher (p &lt; 0.05) in LYC and HYC. The papillary height of the rumen was higher (p &lt; 0.05) in LYC when compared with CON and HYC, whereas rumen wall thickness and muscular layer thickness were higher (p &lt; 0.05) in HYC compared with CON and LYC. The dressing percentage of LYC and HYC was higher (p &lt; 0.05) compared with CON. The diversity, richness and structure of rumen microbiota showed no significant difference (p &gt; 0.05); however, still observed remarkable increases in the relative abundance of several specific genera including Succiniclasticum and Fibrobacter with increasing doses of yeast culture. In addition, at the ASV level, ASV83, ASV123 (Succiniclasticum), and ASV148, ASV250 (Fibrobacter) were increased in YC groups. In conclusion, we confirmed that the supplementation of YC in diet could improve the growth and slaughter performance of fattening Hu sheep through improving nutrient digestion, especially nitrogen utilization, rumen microbial environment and the development of rumen epithelium, which proves the benefits of paraprobiotics in animal production.

Growth, Nutrient Retention, Waste Output, and Antioxidant Capacity of Juvenile Triangular Bream (Megalobrama terminalis) in Response to Dietary Selenium Yeast Concentration.

An 8-week feeding trial was conducted to investigate the effects of different dietary selenium yeast levels on growth, nutrient retention, waste output, and antioxidant capacity of juvenile triangular bream (Megalobrama terminalis). Five isonitrogenous (320 g/kg crude protein) and isolipidic (65 g/kg crude lipid) diets were formulated, with supplementation of graded levels of selenium yeast at 0 (diet Se0), 1 (diet Se1), 3 (diet Se3), 9 (diet Se9), and 12 g/kg (diet Se12). No significant differences were found in initial body weight, condition factor, visceral somatic index, hepatosomatic index, and whole body contents of crude protein, ash, and phosphorus among fish fed different test diet. The highest final body weight and weight gain rate were found in fish fed diet Se3. The specific growth rate (SGR) is closely related to dietary selenium (Se) concentrations with a relationship described as SGR = -0.0043 Se2 + 0.1062 Se + 2.661. Higher feed conversion ratio was found, while lower retention efficiencies of nitrogen and phosphorus were found in fish fed diets Se1, Se3, and Se9 than in fish fed diet Se12. Contents of selenium in whole body, vertebra, and dorsal muscle increased with dietary supplementation of selenium yeast increased from 1 mg/kg to 9 mg/kg. Lower nitrogen and phosphorous waste was found in fish fed diets Se0, Se1, Se3, and Se9 than in fish fed diet Se12. Fish fed diet Se3 exhibited the highest activities of superoxide dismutase, glutathione peroxidase, and lysozyme while the lowest malonaldehyde content in both the liver and kidney. Our results showed that the optimal dietary selenium requirement for triangular bream should be 12.34 mg/kg based on the nonlinear regression on SGR, and fish fed diet Se3 in which selenium concentration (8.24 mg/kg) was close to the optimal requirement displayed the best growth performance, feed nutrient utilization, and antioxidant capacity.

Dietary live yeast supplementation alleviates transport-stress-impaired meat quality of broilers through maintaining muscle energy metabolism and antioxidant status.

BACKGROUNDThis experiment was to investigate the effect of dietary live yeast (LY, 1 × 1010 CFU g−1) supplementation on serum metabolic parameters, meat quality as well as antioxidant enzyme activity of transported broilers. A total of 192 one‐day‐old broilers were randomly assigned to four treatments with six replicates and eight chicks per replicate: a basal diet without transportation (CON), a basal diet containing 0 (T), 500 (T + LY500) and 1000 mg kg−1 (T + LY1000) LY with 3 h of transportation after feeding for 42 days, respectively. The serum and muscle samples of broilers were collected immediately after 3 h of transportation.RESULTSA higher (P < 0.05) final body weight and average daily weight gain were observed in T + LY1000 group compared with CON and T groups. The T + LY1000 group reduced (P < 0.05) the serum lactate contents and improved (P < 0.05) the pH24h and decreased (P < 0.05) the drip loss in muscles of transported‐broilers. Also, the T + LY1000 group enhanced (P < 0.05) the total‐antioxidant capacity and reduced (P < 0.05) the malondialdehyde in serum and muscles. Besides, the messenger RNA (mRNA) expression of avian uncoupling protein (avUCP) in muscles was down‐regulated (P < 0.05) of T + LY1000 group compared with T group.CONCLUSIONDietary LY supplementation alleviates transport‐stress‐impaired meat quality of broilers through maintaining muscle energy metabolism and antioxidant status. Therefore, LY may serve as a potential protector for broilers under transport stress in the future. © 2022 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

Effect of dietary supplementation of Yeast, Garlic and Enzymes on growth performance and economic evaluation in European Eel (Anguilla anguilla) fry

Effect of dietary supplementation of Yeast, Garlic and Enzymes on growth performance and economic evaluation in European Eel (Anguilla anguilla) fry

Dietary chromium yeast supplementation length in diets for growing-finishing pigs

ABSTRACT This study aimed to evaluate different periods of chromium yeast (CrY) supplementation on growth performance and quantitative carcass characteristics of growing-finishing pigs. We used eighty barrows (Duroc/Pietran × Large White/Landrace) with an initial body weight of 24.5±2.4 kg. A completely randomized block design was used within four periods of dietary CrY supplementation (control diet: CrY-free, from 25 to 110 kg; Cr25-110 kg: diet with 0.4 mg kg−1 of CrY, from 25 to 110 kg; Cr50-110 kg: diet with 0.4 mg kg−1 of CrY, from 50 to 110 kg; and Cr70-110 kg: diet with 0.4 mg kg−1 of CrY, from 70 to 110 kg), with ten replicates and two animals each. The CrY supplementation did not affect (P>0.05) either the grow performance or the carcass characteristics evaluated. The dietary supplementation of 0.4 mg kg−1 of CrY for growing-finishing pigs (25 to 110 kg) does not alter the performance neither the quantitative carcass characteristics.

Ruminal pH pattern, fermentation characteristics and related bacteria in response to dietary live yeast (Saccharomyces cerevisiae) supplementation in beef cattle.

ObjectiveIn this study we aimed to evaluate the effect of dietary live yeast supplementation on ruminal pH pattern, fermentation characteristics and associated bacteria in beef cattle.MethodsThis work comprised of in vitro and in vivo experiments. In vitro fermentation was conducted by incubating 0%, 0.05%, 0.075%, 0.1%, 0.125%, and 0.15% active dried yeast (Saccharomyces cerevisiae, ADY) with total mixed ration substrate to determine its dose effect. According to in vitro results, 0.1% ADY inclusion level was assigned in in vivo study for continuously monitoring ruminal fermentation characteristics and microbes. Six ruminally cannulated steers were randomly assigned to 2 treatments (Control and ADY supplementation) as two-period crossover design (30-day). Blood samples were harvested before-feeding and rumen fluid was sampled at 0, 3, 6, 9, and 12 h post-feeding on 30 d.ResultsAfter 24 h in vitro fermentation, pH and gas production were increased at 0.1% ADY where ammonia nitrogen and microbial crude protein also displayed lowest and peak values, respectively. Acetate, butyrate and total volatile fatty acids concentrations heightened with increasing ADY doses and plateaued at high levels, while acetate to propionate ratio was decreased accordingly. In in vivo study, ruminal pH was increased with ADY supplementation that also elevated acetate and propionate. Conversely, ADY reduced lactate level by dampening Streptococcus bovis and inducing greater Selenomonas ruminantium and Megasphaera elsdenii populations involved in lactate utilization. The serum urea nitrogen decreased, whereas glucose, albumin and total protein concentrations were increased with ADY supplementation.ConclusionThe results demonstrated dietary ADY improved ruminal fermentation dose-dependently. The ruminal lactate reduction through modification of lactate metabolic bacteria could be an important reason for rumen pH stabilization induced by ADY. ADY supplementation offered a complementary probiotics strategy in improving gluconeogenesis and nitrogen metabolism of beef cattle, potentially resulted from optimized rumen pH and fermentation.

Effects of dietary live yeast supplementation on growth performance and biomarkers of metabolism and inflammation in heat-stressed and nutrient-restricted pigs.

Study objectives were to determine the effects of dietary live yeast (Saccharomyces cerevisiae strain CNCM I-4407; ActisafHR+; 0.25g/kg of feed; Phileo by Lesaffre, Milwaukee, WI) on growth performance and biomarkers of metabolism and inflammation in heat-stressed and nutrient-restricted pigs. Crossbred barrows (n = 96; 79 ± 1 kg body weight [BW]) were blocked by initial BW and randomly assigned to one of six dietary-environmental treatments: 1) thermoneutral (TN) and fed ad libitum the control diet (TNCon), 2) TN and fed ad libitum a yeast containing diet (TNYeast), 3) TN and pair-fed (PF) the control diet (PFCon), 4) TN and PF the yeast containing diet (PFYeast), 5) heat stress (HS) and fed ad libitum the control diet (HSCon), or 6) HS and fed ad libitum the yeast diet (HSYeast). Following 5 d of acclimation to individual pens, pigs were enrolled in two experimental periods (P). During P1 (7 d), pigs were housed in TN conditions (20 °C) and fed their respective dietary treatments ad libitum. During P2 (28 d), HSCon and HSYeast pigs were fed ad libitum and exposed to progressive cyclical HS (28–33 °C) while TN and PF pigs remained in TN conditions and were fed ad libitum or PF to their HSCon and HSYeast counterparts. Pigs exposed to HS had an overall increase in rectal temperature, skin temperature, and respiration rate compared to TN pigs (0.3 °C, 5.5 °C, and 23 breaths per minute, respectively; P < 0.01). During P2, average daily feed intake (ADFI) decreased in HS compared to TN pigs (30%; P < 0.01). Average daily gain and final BW decreased in HS relative to TN pigs (P < 0.01); however, no differences in feed efficiency (G:F) were observed between HS and TN treatments (P > 0.16). A tendency for decreased ADFI and increased G:F was observed in TNYeast relative to TNCon pigs (P < 0.10). Circulating insulin was similar between HS and TN pigs (P > 0.42). Triiodothyronine and thyroxine levels decreased in HS compared to TN treatments (~19% and 20%, respectively; P < 0.05). Plasma tumor necrosis factor-alpha (TNF-α) did not differ across treatments (P > 0.57) but tended to decrease in HSYeast relative to HSCon pigs (P = 0.09). In summary, dietary live yeast did not affect body temperature indices or growth performance and had minimal effects on biomarkers of metabolism; however, it tended to improve G:F under TN conditions and tended to reduce the proinflammatory mediator TNF-α during HS. Further research on the potential role of dietary live yeast in pigs during HS or nutrient restriction scenarios is warranted.

Influences of Selenium-Enriched Yeast on Growth Performance, Immune Function, and Antioxidant Capacity in Weaned Pigs Exposure to Oxidative Stress.

This study elucidated the function role of dietary selenium-enriched yeast (SeY) supplementation on growth performance, immune function, and antioxidant capacity in weaned pigs exposure to oxidative stress. Thirty-two similarity weight pigs were randomly divided into four treatments: (1) nonchallenged control, (2) control+SeY, (3) control+diquat, and (4) control+SeY+diquat. The period of experiment was 21 days; on day 16, pigs were injected with diquat or sterile saline. Results revealed that oxidative stress was notably detrimental to the growth performance of piglets, but SeY supplementation ameliorated this phenomenon, which might be regarding the increasing of body antioxidant capacity and immune functions. In details, SeY supplementation improved the digestibility of crude protein (CP), ash, and gross energy (GE). Moreover, the serum concentrations of proinflammatory cytokines (TNF-α, IL-1β, and IL-6), glutamic-pyruvic transaminase(GPT), and glutamic-oxaloacetic transaminase (GOT) were reduced via SeY supplemented, and serum concentrations of immunoglobulins A (IgA), IgG, and activities of antioxidant enzymes such as the superoxide dismutase (SOD), catalase (CAT) ,and glutathione peroxidase (GSH-Px) were improved in the diquat-challenged pigs (P < 0.05). In addition, SeY supplementation acutely enhanced the activities of these antioxidant enzymes in the liver and thymus upon diquat challenge, which involved with the upregulation of the critical genes related antioxidant signaling such as the nuclear factor erythroid-derived 2-related factor 2 (Nrf-2) and heme oxygenase-1 (HO-1) (P < 0.05). Importantly, we also found that SeY supplementation apparently reduced the malondialdehyde (MDA) concentrations in the liver, thymus, and serum (P < 0.05). Specifically, the expression levels of TNF-α, IL-6, IL-1β, Toll-like receptor 4 (TLR-4), and nuclear factor-κB (NF-κB) in the liver and thymus were downregulated by SeY upon diquat challenge. These results suggested that SeY can attenuate oxidative stress-induced growth retardation, which was associated with elevating body antioxidant capacity, immune functions, and suppressed inflammatory response.

Dietary supranutritional supplementation of selenium-enriched yeast improves feed efficiency and blood antioxidant status of growing lambs reared under warm environmental condition.

The objective of this study was to investigate the impacts of dietary supranutritional supplementation of selenium-yeast (SY) on growth performance, blood cells, antioxidant status, and metabolic profile of lambs. Twenty-one Kermani male lambs (28.5 ± 2.6kg of body weight) were used in a completely randomized design for 8weeks under warm condition with temperature-humidity index (THI) of 81.3 ± 0.37 unit. The lambs were randomly divided into 3 groups given the basal diet either un-supplemented (control group) or supplemented with 0.6 or 1.2mg of Se/kg dry matter (DM) as SY. Average daily Se intake was 0.12, 0.83, and 1.54mg in lambs on control, 0.6mg and 1.2mg of supplemental Se treatments, respectively (P< 0.0001). The results showed that average daily gain tended to be higher in lambs received Se-supplemented diets than those fed the unsupplemented diet (P < 0.1). Feed efficiency improved by SY supplementation, thereby feed to gain ratio (FG) reduced 19 and 17% respectively in lambs fed 0.6 (FG = 6.58) and 1.2 (FG = 6.7) mg of Se/kg DM compared to those of the control (FG = 8.12) (P < 0.05). Feeding 1.2mg of Se to growing lambs not only improved blood total antioxidant capacity (T-AOC) as 18.1% and glutathione peroxidase activity (GSH-Px) as 17.6%, but also made 13.1% reduction in serum malondialdehyde (MDA) concentration as a lipid peroxidation marker (P < 0.05). Despite its statistical insignificance (P > 0.05), erythrocyte superoxide dismutase (SOD) activity was also greater in Se-fed groups compared to that of the control. Based on the obtained data, lambs fed 1.2mg of Se/kg DM, had lower serum urea concentration and albumin to globulin ratio than those on unsupplemented diet (P < 0.05). Moreover, increased RBC count was observed in lambs received 0.6mg of supplemental Se (P < 0.05). Selenium-enriched yeast supplementation gave rise to increased blood lymphocyte percentage (P < 0.05). The results also indicated that dietary high Se feeding had no adverse effects on blood metabolites including glucose, total protein, albumin, globulin, liver enzymes, and triglyceride content. In the overall, these findings suggest that Se-enriched yeast is a kind of safe Se source for sheep and its dietary supranutritional supplementation for 8weeks improves feed efficiency of growing lambs. Furthermore, increasing the supplemental Se to 1.2mg/kg of diet promotes lambs' blood antioxidant status without inducing any detrimental impacts on cell metabolism.