Manufacturers Of Dietary Supplements Research Articles

Lack of genotoxicity potential and safety assessment of 4-(2-hydroxyethyl) morpholine present as an impurity in pharmaceuticals and nutritional supplements

4-(2-Hydroxyethyl) morpholine (HEM) is widely used as a building block of macromolecules in the manufacture of pharmaceuticals and dietary supplements and could remain as an impurity in the finished products. An evaluation of HEM was conducted to identify endpoints that could be used to determine the point-of-departure (POD) for use in assessing the potential risk from exposure to HEM. No oral repeated dose toxicological studies of appropriate duration were found for HEM. Therefore, suitable analogue(s) were identified. Although oral repeated dose studies were available for the analogues, the studies were not of sufficient duration for use in the assignment of a POD for risk evaluation. Accordingly, the Threshold of Toxicological Concern (TTC) approach, which proposes that a de minimis value can be derived to qualitatively assess risk, was considered for HEM. To determine the appropriate TTC approach (genotoxic or non-genotoxic), the genotoxicity of HEM and its analogues were evaluated. The weight of the evidence indicated that HEM, and the appropriate analogues, are not genotoxic. Considering the chemical structure of HEM, the non-genotoxic Cramer class III TTC value of 1.5 μg/kg bw/day was determined to be appropriate for use in safety assessment of HEM as an impurity in products intended for human consumption.

Variation of Saponarin Content in Barley Sprouts (Hordeum vulgare L.) by Natural Light Shielding: Implication of the Importance of Light Intensity.

Saponarin is a functional metabolite produced by barley sprouts, and the mass production of saponarin by this crop is attractive for dietary supplement manufacturing. Light is the most important environmental factor determining plant growth, survival, and the production of secondary metabolites including flavonoids. This study was conducted to investigate the importance of light intensity for saponarin production in barley sprouts using a hydroponic growth system. Light intensity was manipulated by using shielding treatments to 100, 80, 70, and 50% natural sunlight (NS), and crop cultivation was performed on a monthly cycle. We found that the growth rate and biomass of barley sprouts did not differ in response to the shield treatments, whereas the saponarin content did. The highest saponarin content (i.e., from 1329 to 1673 mg 100 g-1) was observed in the 100% NS treatment, and it gradually decreased as light intensity also decreased. Statistical analysis revealed a significant polynomial relationship of saponarin content with cumulative PPFD (R2 = 76%), implying that the absolute total amount of light exposure over the growth period has a large effect on saponarin productivity in a hydroponic facility. Taken together, our results showed that shielding conditions, which are often unintentionally created by the design of cultivation facilities, can adversely affect saponarin production in barley sprouts. In addition, it was confirmed through our findings that light conditions with at least 70% NS in the cultivation facility enable the production of an amount corresponding to the saponarin content of the sprouts (>1000 mg 100 g-1) produced in the open field. Further studies are needed to investigate the underlying physiological and molecular mechanisms responsible for the relationship of saponarin content with light quantity and quality in barley sprouts.

Optimizing pricing and inventory strategies for dietary supplement production under stochastic demand

PurposeThe increasing popularity of ERP solutions has provided dietary supplement manufacturing companies with modules to manage pricing and inventory. However, the decisions made by these modules are often independent and rely on deterministic forecasts. This paper studies a multi-product dietary supplement manufacturing system under stochastic demands. The purpose is to maximize the long-run expected profit by jointly considering pricing and inventory strategies.Design/methodology/approachThe authors investigate both the general cases and three special cases including stable demand, negligible backlog and instantaneous replenishment. A two-stage algorithm named PAS is proposed. In the strategy construction stage, the constructed objective bounds are combined to provide estimates which then help to derive the optimal product prices. In the system operation stage, replenishment decisions are further made based on the prices generated from the previous stage.FindingsIt is proved that base-stock policy is optimal for the studied system, and the optimal based-stock level is provided. The global optimal strategies are obtained for three important special cases. For the general case, theoretical objective bounds are established. These bounds provide quick and reliable performance estimates for practical applications.Originality/valueVery few studies have jointly considered pricing and inventory strategies with uncertainty demands in the dietary supplement industry. The PAS algorithm developed integrates these decisions and consistently generates high-quality solutions even under highly varying demands. Such algorithm could be a valuable add-on to the pricing and inventory management modules in ERP systems.

Optimizing the Relationship between Regulation and Innovation in Dietary Supplements: A Case Study of Food with Function Claims in Japan.

Regulation has long been a counterpart of innovation in the health care industry, and recent cases have demonstrated that appropriately designed regulations can both coexist with and promote innovation. This study is the first study to explore how the regulatory environment affected the innovation process during the transition of the regulations for functional foods in Japan by examining quantitatively the impact of the foods with function claims (FFC) system on industry, companies, and products. Based on a dataset of Japanese dietary supplement manufacturing companies (n = 169) and their products (n = 731) in 2019, we found that companies that have newly entered the FFC system are smaller in scale than existing companies (p < 0.01, Wilcoxon rank sum test). We also found that companies with FFC products have larger revenue growth (p = 0.01). A multiple regression analysis revealed that FFC product sales increased with in-house clinical testing (coefficient: 26.8, p < 0.0001), diverse active ingredients (coefficient: 7.6, p < 0.001), and the claim of new functions (coefficient: 10.2, p < 0.05). These results suggested that the FFC system facilitated the market entry of small and mid-size enterprises and promoted the creation of high-value products through innovative company efforts.

Chiral separation of α-tocopherol and α-tocopheryl acetate by supercritical fluid chromatography for accurate vitamin E nutrition labeling

Separations of respective stereoisomers of α-tocopherol and α-tocopheryl acetate by supercritical fluid chromatography (SFC) using polysaccharide-based chiral stationary phases (CSP) were studied. Two separate methods have been developed for α-tocopherol and α-tocopheryl acetate with total runtime of 35 min and 15 min, respectively. Under the optimized conditions, all-rac-α-tocopherol is separated into four peaks (peak area ratio 5:1:1:1) on an amylose tris(3,5-dimethylphenylcarbamate) CSP, and all-rac-α-tocopheryl acetate is separated into 2 peaks with equal peak areas on a cellulose tris(3,5-dimethylphenylcarbamate) CSP. The characteristic peak profiles of these racemic mixtures are distinctive enough to differentiate synthetic vitamin E (racemate) from the naturally occurring vitamin E (RRR-stereoisomer only). The α-tocopherol method has been demonstrated free of interference from other natural tocopherols, such as β-, γ-, and δ-tocopherol. Evaluation of the α-tocopherol method with commercially available vitamin E dietary supplements and an infant formula showed acceptable results in the qualitative analysis of α-tocopherol. These SFC methods provide fast, simple, and “green” solutions to verify or determine the type of vitamin E for dietary supplement and food manufacturers to ensure accurate vitamin E nutrition label claims.

The knowledge and attitudes of pharmacists related to the use of dietary supplements: An observational study in northeastern Italy

Inappropriate use of dietary supplements can be potentially harmful to patients, especially given the uncontrolled information on the Internet and social media. The role that pharmacists play in advising citizens and purchasing these products can help improve their safer use, but the knowledge, attitudes, and practices of these professionals on this topic are still largely unknown.Pharmacists in the Italian Region of Friuli-Venezia Giulia (FVG) were surveyed from September 2020 to February 2021 using a 54-item online questionnaire. The questions were related to knowledge, attitudes, and practice. Data on gender, age, work experience, degree, and function in pharmacy were also collected.232 questionnaires were completed, predominantly by women (71 %). The majority of respondents had a degree in Pharmacy (77 %) and were employed in a pharmacy (66 %) where they had worked for at least 10 years (61 %). Pharmacists’ knowledge was rated as low, with one-third of participants above the median; knowledge was higher among more experienced pharmacists. Larger knowledge gaps were noted regarding the potential carcinogenicity of multivitamin misuse and the undesirable presence of unlabeled ingredients in dietary supplements. Employees were more likely than owners to report purchasing dietary supplements at the direct request of patients (p < 0.05).The low level of pharmacists’ knowledge about the use of dietary supplements is unsatisfactory, with concerning impact on patient safety. Young professionals in particular would benefit from continuous education to better advise patients. Nevertheless, the European regulatory framework for the manufacture, sale and post-marketing surveillance of dietary supplements needs to be strengthened.

Herb-drug interaction resources: The PHYDGI database

BackgroundAccording to the WHO, about 60% of the world's population currently uses traditional herbal remedies as complementary or alternative medicine and 80% of the population in developing countries depends almost totally on them for their primary health-care requirements. Due to a lack of knowledge on the action mechanisms of plant compounds or mixture, concerns regarding the safety of potential Herb-Drug Interactions (HDIs) have been raised. Over the past two decades and by 2018, 124 WHO Member States had implemented regulations on herbal medicines. However, databases of interactions between plants and modern allopathic drugs are still limited. MethodsThis study aims to build a database containing the information required for the proper concomitant use of chemical drugs and plant remedies. To develop the HDI database, scientific research articles were searched online using Medline, Scopus and Google scholar databases. Specific key-words, including plant names and “herb” “drug” “interactions” were questioned. Articles were then selected on the availability of required information including the fine identification of the plant extracts. Peculiar attention was paid on data reliability, and redundancies in interaction data increased their credibility. To inform about this reliability, a specific grade was applied. Besides, a grade based on the strength of pharmacokinetic changes was also given. ResultsAs of February 2022, the PHYDGI database allows health care professionals, researchers and manufacturers of plant-based remedies and dietary supplements to search an entity amongst 58 plants and its potential interactions with a given drug amongst 114 commonly used medical substances. ConclusionThe PHYDGI database integrates scientific data on HDI in well-structured forms including reference to the scientific sources. It provides herbal entities and their interactions as well as the level of strength for the pharmacokinetic interactions. This database which will be available for health practitioners, for phyto-drugs and food supplement manufacturers should help reducing potential HDI and developing safe use of plant-based remedies or supplements.

ProBioQuest: a database and semantic analysis engine for literature, clinical trials and patents related to probiotics.

The use of probiotics to improve health via the modulation of gut microbiota has gained wide attention. The growing volume of investigations of probiotic microorganisms and commercialized probiotic products has created the need for a database to organize the health-promoting functions driven by probiotics reported in academic articles, clinical trials and patents. We constructed ProBioQuest to collect up-to-date literature related to probiotics from PubMed.gov, ClinicalTrials.gov and PatentsView. More than 2.8 million articles have been collected. Automated information technology-assisted procedures enabled us to collect the data continuously, providing the most up-to-date information. Statistical functions and semantic analyses are provided on the website as an advanced search engine, which contributes to the semantic tool of this database for information search and analyses. The semantic analytical output provides categorized search results and functions to enhance further analysis. A keyword bank is included which can display multiple tables of contents. Users can select keywords from different displayed categories to achieve easily filtered searches. Additional information on the searched items can be browsed via the link-out function. ProBioQuest is not only useful to scientists and health professionals but also to dietary supplement manufacturers and the general public. In this paper, the method we used to build this database-web system is described. Applications of ProBioQuest for several literature-based analyses of probiotics are included as examples of the various uses of this search engine. ProBioQuest can be accessed free of charge at http://kwanlab.bio.cuhk.edu.hk/PBQ/.Database URL http://kwanlab.bio.cuhk.edu.hk/PBQ/

Mass Spectrometry Metabolomics and Feature-Based Molecular Networking Reveals Population-Specific Chemistry in Some Species of the Sceletium Genus.

The Sceletium genus has been of medicinal importance in southern Africa for millennia and Sceletium tortuosum (Aizoaceae), one of eight species in the genus has gained pharmaceutical importance as an anxiolytic and anti-depressant due to the presence of mesembrine alkaloids. S. tortuosum is used for the manufacture of herbal teas, dietary supplements and other phytopharmaceutical products. This study aimed to provide a metabolomic characterization of S. tortuosum and its sister species as these are not easy to distinguish using morphology alone. Plant samples were thus collected from various locations in the succulent Karoo (South Africa) and analyzed through liquid chromatography-mass spectrometry (LC-MS), using MSE fragmentation as a putative tool for chemical identities. Metabolomics-based analyses in combination with molecular networking were able to distinguish between the four species of Sceletium based on the presence of 4-(3,4-dimethyoxyphenyl)-4-[2-acetylmethlamino)ethyl]cyclohexanone (m/z 334.2020; RT 6.60 min), mesembrine (m/z 290.1757; RT 5.10 min) and 4'-O-demethylmesembrenol (m/z 276.1597; RT 4.17 min). Metabolomic profiles varied according to the different localities and metabolites occurred at variable quantitative levels in Sceletium ecotypes. Molecular networking provided the added advantage of being able to observe mesembrine alkaloid isomers and coeluting metabolites (from the joubertiamine group) that were difficult to discern without this application. By combining high-throughput metabolomics together with global and feature based-molecular networking, a powerful metabolite profiling platform that is able to discern chemical patterns within and between populations was established. These techniques were able to reveal chemotaxonomic relationships and allowed for the discovery of chemical markers that may be used as part of monitoring protocols during the manufacture of phytopharmaceutical and dietary products based on Sceletium.

Development of Kudzu (Pueraria Montana var. lobata) Reference Materials for the Determination of Isoflavones and Toxic Elements.

In collaboration with the Office of Dietary Supplements at the National Institutes of Health, the National Institute of Standards and Technology issued a suite of botanical matrix reference materials (RMs) and Standard Reference Material® (SRM) for determination of isoflavones and toxic elements in kudzu dietary supplement ingredients. RM 8650 Pueraria montana var. lobata (Kudzu) Rhizome, SRM 3268 Pueraria montana var. lobata (Kudzu) Extract, and RM 8652 Kudzu-Containing Solid Oral Dosage Form were issued with values assigned for isoflavones (puerarin, daidzin, and daidzein), toxic elements (arsenic, cadmium, and lead), and selenium. Isoflavone values were assigned using liquid chromatography with UV absorbance or mass spectrometry detection. Element values were assigned using inductively coupled plasma mass spectrometry and results from an interlaboratory comparison exercise. Mass fractions for puerarin were 32.2 ± 3.2 mg/g, 128 ± 13 mg/g, and 68.2 ± 6.9 mg/g in RM 8650, SRM 3268, and RM 8652, respectively. Arsenic increases from 156 ± 14 ng/g to 849 ± 83 ng/g and cadmium decreases from 348 ± 14 ng/g to 82.1 ± 4.9 ng/g from rhizome to extract. The kudzu RM/SRM suite complements previously issued soy-related SRMs with values assigned for isoflavones, which have been studied for their potential health benefits, and expands the analytical resource by providing values for puerarin, an isoflavone not found in soy. The three new kudzurmaterials are for use in the determination of isoflavones, toxic elements, and selenium. For the isoflavones, these new kudzu materials provide higher levels of daidzin and daidzein than existing soy-related SRMs, and they provide a value for an isoflavone not in existing SRMs (puerarin). Toxic elements in RM 8650 and SRM 3268 provide new botanical matrixes for use by dietary supplement manufacturers for the verification of the safety of their raw materials.

Chemical, Manufacturing, and Standardization Controls of Grape Polyphenol Dietary Supplements in Support of a Clinical Study: Mass Uniformity, Polyphenol Dosage, and Profiles.

Bioactive dietary polyphenols in grape (Vitis vinifera) have been used in Dietary Supplements (DSs) with the aim to prevent numerous diseases, including cardiovascular and neurodegenerative diseases, and to reduce depression and anxiety. Given prior recognition that DSs can be quality challenged from the purity, authentication, adulteration, and actual concentration of targeted bioactives, to ensure consumer health protection as well as the quality and safety of grape polyphenol-based DSs, the present investigation was aimed at establishing a comprehensive quality control (QC) approach for grape polyphenol-based DSs in support of a human clinical study. In this study, the manufactured grape seed polyphenol extract (GSPE) and trans-resveratrol (RSV) capsules and Concord Grape Juice (CGJ) along with the corresponding original drug materials were analyzed using the developed different liquid chromatography/UV-visible spectroscopy/mass spectrometry (LC/UV-Vis/MS) methods. The weight variation of GSPE and RSV capsules was also evaluated according to the US Pharmacopeia (USP) tests. The results indicate that the total identified polyphenol content in each grape seed extract (GSE) capsule/CGJ is very similar and all GSE/RSV capsules pass the content/weight uniformity test. Given the complexity of these and many botanical products from the issues of purity, quality, adulteration, consistency, and their coupling to the complex chemistry in each grape-derived botanical, quality assurance and the steps needed to ensure grape-derived DSs being well homogeneous and stable and containing the known and expected bioactives at specific concentration ranges are fundamental to any research study and in particular to a clinical trial. Each of these issues is essential to provide a solid foundation upon which clinical trials with botanicals can be conducted with the goal of realizing measurable mental health outcomes such as reducing depression and anxiety as well as understanding of their underlying biological mechanisms.

Are Salix purpurea L. genotypes from natural locations promising candidates for the production of high-quality herbal raw materials under controlled conditions?

Recent years have witnessed a considerable increase in demand for natural plant-based raw materials, which is why the agricultural production of bioresources on arable or marginal land is not only a viable alternative, but also an important direction for the development of modern agriculture. The present study focuses on evaluating the suitability of Salix purpurea L. genotypes from different natural locations for the agricultural production of high-quality herbal raw materials used in the manufacture of drugs, dietary supplements, cosmetics, biological preparations and animal feed. High variation was noted in the analyzed traits of S. purpurea (plant height, stem diameter, number of shoots per plant and plant weight) and leaf rust infection rates. Two S. purpurea genotypes were characterized by highly satisfactory values of yield-related traits and a high content of salicylic glycosides (SG) in the bark (109.3 mg g−1 and 86.3 mg g-1, respectively), regardless of plant age, they were free of infection caused by fungi of the genus Melampsora. Due to their high potential for the agricultural production of high-quality herbal raw materials, they constitute promising source material for S. purpurea cultivation under controlled conditions. The above genotypes were registered as cultivars ASPI and ASPIRA by the Research Center for Cultivar Testing (COBORU). The results of this study indicate that S. purpurea genotypes from natural locations can be used for genetic recombination in targeted plant breeding.

Knowledge, Attitudes, and Practices Related to Dietary Supplementation, before and during the COVID-19 Pandemic: Findings from a Cross-Sectional Survey in the Lebanese Population.

At the start of 2020, a new coronavirus (COVID-19) invaded the world leading to the death of 3.92 million people. Sadly, to date, no remedy has been discovered for this virus. Preventive vaccines have been under investigation, but were unavailable until December 2020. Clinical deficiencies of nutrients may increase susceptibility to infections. This knowledge may have provided an incentive for some dietary supplement (DS) manufacturers to advertise their products as COVID-19 preventatives or cures without any substantiation, targeting mainly social media fans. The objective of this research was to assess the usage, knowledge and attitudes towards dietary supplementation before and during the COVID-19 pandemic among Lebanese people. A cross-sectional study was conducted based on a convenience sample (N = 2966) and information from participants aged 18 years and above was collected about periods before and during the pandemic. Our findings showed that attitudes towards DSs changed when the pandemic emerged and people believed that DSs can improve their health and strengthen their immunity. Despite the rise in DS prescription by healthcare professionals, the prevalence of DS use decreased from 73.3% before the pandemic to 69.9% during the pandemic (p < 0.001). Study results declared that the weekly or the daily estimated intake had increased during the pandemic as compared to before the pandemic, from 14% to 15.6% for antioxidants (p = 0.014), from 35.3% to 42.1% for vitamin C (p < 0.001), from 35.5% to 41% for vitamin D (p < 0.001), from 15.2% to 17.5% for vitamin E (p = 0.002), and from 18.8% to 29.3% for zinc (p < 0.001) and other vitamins and minerals (from 9% to 10.9%, p < 0.001). Binary logistic regression indicated that the use of DSs during the pandemic was 4 times higher among those infected with COVID-19, 30 times higher among those who used to take DSs before pandemic, and 1.5 times higher among those who worked in the medical sector. To conclude, there is a crucial need to increase awareness among Lebanese people regarding the use of DSs.

Ex Vivo Antioxidant Capacities of Fruit and Vegetable Juices. Potential In Vivo Extrapolation.

Background: In support of claims that their products have antioxidant properties, the food industry and dietary supplement manufacturers rely solely on the in vitro determination of the ORAC (oxygen radical antioxidant capacity) value, despite its acknowledged lack of any in vivo relevance. It thus appears necessary to use tests exploiting biological materials (blood, white blood cells) capable of producing physiological free radicals, in order to evaluate more adequately the antioxidant capacities of foods such as fruit and vegetable juices. Materials: Two approaches to assessing the antioxidant capacities of 21 commercial fruit and vegetable juices were compared: the ORAC assay and the “PMA–whole blood assay,” which uses whole blood stimulated by phorbol myristate acetate to produce the superoxide anion. We described in another paper the total polyphenol contents (TPCs) and individual phenolic compound contents of all the juices were investigated. Results: Ranking of the juices from highest to lowest antioxidant capacity differed considerably according to the test used, so there was no correlation (r = 0.33, p = 0.13) between the two assays when considering all juices. Although the results of the ORAC assay correlated positively with TPC (r = 0.50, p = 0.02), a much stronger correlation (r = 0.70, p = 0.004) emerged between TPC and % superoxide anion inhibition. In the PMA–whole blood assay, peonidin-3-O-glucoside, epigallocatechin gallate, catechin, and quercetin present in juices were found to inhibit superoxide anion production at concentrations below 1 µM, with a strong positive correlation. Conclusions: Associated with the determination of total and individual phenolic compounds contained in fruit and vegetable juices, the PMA–whole blood assay appears better than the ORAC assay for evaluating juice antioxidant capacity.

Ganoderma sessile is a fast polysaccharide producer among Ganoderma species

ABSTRACT The selection of fast-growing and high-yield-producing strains is required to satisfy the market demand on fungal food supplements. To that aim, three strains deposited in our collection as G. lucidum and G. oregonense were screened for polysaccharide production and biomass yield. Ganoderma strains deposited as G. lucidum were identified as G. sessile and G. lingzhi by nuc rDNA internal transcribed spacer ITS1-5.8S-ITS2 (ITS) and translation elongation factor 1-α (TEF1-α) phylogenies. The identity of G. oregonense was confirmed by molecular phylogeny and biogeography. Additionally, mycelial antagonism confirmed species differentiation, and strains were further distinguished by morphology and protein profiles. Biomass and polysaccharide yields of G. sessile were clearly different from those of G. lingzhi and G. oregonense in both liquid culture and solid-state fermentation. The maximum polysaccharide yield (4.52 ± 0.83 g L−1) for G. sessile was obtained from submerged cultures at day 9. G. sessile also achieved the highest linear growth in lignocellulosic solid substrates. Consequently, basidiomata were successfully obtained by solid-state fermentation in polypropylene bags, whereas G. lingzhi and G. oregonense mushrooms were not produced in artificial solid substrates. G. sessile, a species frequently collected in America, showed to be a promising polysaccharide producer for the manufacture of dietary supplements.

Overview on Microbial Enzymatic Production of Algal Oligosaccharides for Nutraceutical Applications.

Global requirement for algal foods is increasing, as they are progressively consumed for its nutrition and health. Macroalgae is a proven source of metabolites, proteins, pigments, bioactive compounds, and algal polysaccharides. The unique polysaccharides such as agar, carrageenan, porphyran, alginate, fucoidan, laminarin, and ulvan are known for its wide range of bioactivities and extensively used for applications from tissue engineering to drug delivery. However, there are few limitations due to its high molecular size, low compatibility, and hydrocolloid nature. Hence, the enzymatically produced algal oligosaccharides have drawn tremendous attention due to its green synthesis, solubility, and lower molecular size. They are reported to have bioactivities including antioxidant, antiglycemic, immunostimulatory, anti-inflammatory, and prebiotic activities, which can be used in the healthcare and nutraceutical industry for the manufacture of functional foods and dietary supplements. However, identification of potential microorganisms, producing polysaccharide hydrolyzing enzymes, remains a major bottle neck for efficient utilization of bioactive algal oligosaccharides. This review summarizes the recent developments in the identification and characterization of microbial enzymes for the production of bioactive algal oligosaccharides. This can improve our understanding of bioactive algal oligosaccharides and pave way for efficient utilization of macroalgae to prevent various chronic diseases.

Using “Text Mining” Analysis for the Assessment of the Health Quality of Dietary Supplements

Abstract Techniques of text data analysis have been known for many years and commonly used in many areas of life. Text mining enables, among others, the acquisition of information from the text, its filtering, and studying of similarities and relationships. The aim of this paper is to design a method that would make it possible to assess the health quality of dietary supplements, on the basis of text mining techniques. A fictional plant-based product was used in the study, which was compared with other products containing at least one of the tested ingredients registered in the years 2007–2019 in the register of dietary supplements kept by the Chief Sanitary Inspectorate (GIS), which were given either the “consistent” or “to be clarified” status. The obtained results concern the frequency of occurrence of the individual ingredients (St John’s wort/Hypericum, melissa, rose root/Rhodiola) in other products, considering their status in the register. The data thus obtained was subjected to classical statistical analysis in order to find correlations between the presence of a given ingredient and the product status. In view of the obtained results, the text mining analysis may be considered as a helpful tool in the process of internal risk assessment performed by manufacturers of dietary supplements.

Subcritical dimethyl ether extraction as a simple method to extract nutraceuticals from byproducts from rice bran oil manufacture

The byproducts of rice bran oil processes are a good source of fat-soluble nutraceuticals, including γ-oryzanol, phytosterol, and policosanols. This study aimed to investigate the effects of green technology with low pressure as the subcritical fluid extraction with dimethyl ether (SUBFDME) on the amount of γ-oryzanol, phytosterol, and policosanol extracted from the byproducts and to increase the purity of policosanols. The SUBFDME extraction apparatus was operated under pressures below 1 MPa. Compared to the chemical extraction method, SUBFDME gave the highest content of γ-oryzanol at 924.51 mg/100 g from defatted rice bran, followed by 829.88 mg/100 g from the filter cake, while the highest phytosterol content was 367.54 mg/100 g. Transesterification gave the highest extraction yield of 43.71% with the highest policosanol content (30,787 mg/100 g), and the SUBFDME method increased the policosanol level from transesterified rice bran wax to 84,913.14 mg/100 g. The results indicate that the SUBFDME method is a promising tool to extract γ-oryzanol and phytosterol and a simple and effective technique to increase the purity of policosanol. The study presented a novel technique for the potential use of SUBSFDME as an alternative low-pressure and low-temperature technique to extract γ-oryzanol and phytosterol. The combination of transesterification and the SUBFDME technique is a potential simple two-step method to extract and purify policosanol, which is beneficial for the manufacture of dietary supplements, functional foods and pharmaceutical products.

Probiotics: Promise, Evidence, and Hope

Probiotics: Promise, Evidence, and Hope

IS IT TIME TO REGULATE THE DIETARY SUPPLEMENT INDUSTRY?

ABSTRACT This paper focuses on the regulatory state of dietary supplements, specifically in the United States, with respect to the current safety and advertising practices of dietary supplement manufacturers, sellers, and distributors. It will delve into existing dishonest and dangerous practices relating to the selling and marketing of dietary supplements, while exposing the various risks these practices have on the buyers, sellers, manufacturers, and the industry in general. The central message of this study is that there is a great need for honesty in advertising and more safety in the products sold as dietary supplements, a need which can, perhaps, only be met through more stringent FDA regulations. The paper summarizes findings from the latest data and puts forward practical suggestions for regulators, suppliers, distributors, and consumers alike. Keywords Dietary supplements; honesty in advertising; product safety, ethics