Dietary Replacement Of Soybean Meal Research Articles

Dietary Replacement of Soybean Meal with Zanthoxylum bungeanum Seed Meal on Growth Performance, Blood Parameters, and Nutrient Utilization in Broiler Chickens.

Zanthoxylum bungeanum seed meal (ZBM), a novel plant protein raw material, has shown promising potential in enhancing the growth of broiler chickens as a substitute for soybean meal (SBM) in feed. In the artificial digestive experiment of vitro experiments, the digestibility of ZBM and SBM were assessed using the SDS-III Single Stomach Animal Biometric Digestion System. Subsequently, 180 1-day old AA chicks were divided into three groups for in vivo experiments: corn-soybean-meal-based diet (CON group); ZBM replacing 5% soybean meal in the basal diet (ZBM-1 group); ZBM replacing 10% soybean meal in the basal diet (ZBM-2 group). The experiment period lasted for 42 days. Compared to SBM, ZBM demonstrated higher crude protein content, dry matter digestibility, and extracorporeal digestible protein. Compared with the CON group, the broilers in the ZBM-2 group showed improved ADG and ADFI during the 1-21 d, 22-42 d, and 1-42 d periods (p < 0.05). Furthermore, the ZBM groups exhibited significant increases in slaughter performance compared with the CON group (p < 0.05). The substitution of ZBM for SBM also leads to a significant reduction in serum enzyme indicators (p < 0.05). Additionally, the lipoprotein and total cholesterol of the ZBM groups were significantly lower than those of the CON group (p < 0.05). Substituting SBM with ZBM significantly enhances the activity of superoxide dismutase and the content of immunoglobulin G in broiler serum, while reducing the content of malondildehyde (p < 0.05). The ZBM groups showed significantly higher utilization of dry matter, crude protein, and energy compared with the CON group (p < 0.05). In conclusion, the study confirmed that the substitution of SBM with 5-10% ZBM in broiler diets has a significant positive effect on growth, development, antioxidant capacity, immune function, and nutrient utilization. This study not only provides a theoretical foundation for the utilization of ZBM in broiler diets but also offers an effective approach for reducing reliance on soybean meal.

Dietary replacement of soybean meal with heat-treated grain soybean in diets of feedlot-finished beef cattle: impacts on intake, digestibility, and ruminal parameters.

The objective of this study was to assess the impact of increasing levels of heat-treated soybean in the diet of crossbred cattle during the finishing phase on nutrient intake and digestibility, ruminal parameters, digesta passage rate, nitrogen balance, and microbial protein synthesis. Five steers, crossbred 7/8 Jersey x Zebu, fitted with rumen cannulas and with an average weight of 350 ± 50kg, were utilized. The experimental treatments consisted of 0, 7, 14, 21, and 28% inclusion of heat-treated soybean in the total diet dry matter. The animals were randomly allocated in a 5 × 5 Latin square design. Evaluation of the animals took place over five experimental periods, each lasting 20days. During each experimental period, the first 15days were allocated for animal adaptation to the experimental diets, followed by five days of data collection. No significant differences were observed among the diets in terms of dry matter intake (average of 6.57kgday-1; P = 0.615) and organic matter intake (average of 6.23kgday-1; P = 0.832). However, heat-treated soybean had a significant impact on the digestibility of dry matter (P = 0.02), organic matter (P = 0.01), crude protein (P < 0.01), and neutral detergent fiber (P < 0.01). There was no observed change on microbial protein synthesis (average of 409.6gday-1) in animals with the inclusion of heat-treated soybean in the diets. With each 1% inclusion of heat-treated soybean in the cattle diet, there was an increase of 0.00754 units in ruminal pH values and a reduction of 0.75839mg dL-1 in ruminal ammoniacal nitrogen values. This study suggests that heat-treated soybean can be included in up to 15% of the dry matter in diets for finishing feedlot cattle.

Dietary replacement of soybean meal with black soldier fly larvae meal in juvenile Labeo rohita and Catla catla: Effects on growth, nutritional quality, oxidative stress biomarkers and disease resistance.

This experiment aimed to investigate the effects of partial substitution of crude protein from soybean meal (SBM) with black soldier fly (Hermetia illucens) larvae meal (BSFLM) in juvenile rohu (Labeo rohita) and catla (Catla catla). Four isonitrogenous diets (23% crude protein) were formulated to replace 0% (T0), 40% (T40), 80% (T80) and 100% (T100) crude protein from SBM with BSFLM. Triplicate groups of each species (10 fish per replicate) were fed in an eight week growth experiment. After final sampling (n = 20 fish per dietary group), the remaining fish were exposed to bacterial (Staphylococcus aureus) challenge (0.80 CFU/ml) for 15 days. Rohu fed with BSFLM substituted diets showed significantly higher growth and feed conversion ratio as compared to those in T0. Catla fed with BSFLM substituted diets showed slightly higher growth indices. The growth response of rohu to BSFLM substitution was better than that noted in catla in all groups. The chemical composition, amino acids and fatty acids profile, haematological and biochemical parameters, levels of liver function enzymes measured in T0, T40, T80 and T100 were similar between four dietary groups in both species. However, the maximum value of cholesterol and triglycerides were noted in T100 both in catla and rohu. The values of lauric acid, α-linolenic acid, decosahexanoic acid, n3:n6 fatty acids ratio progressively increased with dietary increase of BSFLM in both species. At end of the growth experiment, the levels of catalase, superoxide dismutase and lysozyme increased linearly with the inclusion of BSFLM in both species while malondialdehyde showed similar values between different groups. However, catalase, and superoxide dismutase increased (T0<T40<T80<T100) in both rohu and catla after exposure to bacterial challenge while malondialdehyde remained almost the same. These biomarkers indicate that substitution of SBM with BSFLM up to 100% improved disease resistance in both species against gram-positive bacteria.

Dietary replacement of soybean meal with black soldier fly larvae meal improves growth and disease resistance in juvenile Pangasius bocourti

Abstract This eight week long experiment was performed to study the effects of substitution of crude protein from soybean meal (SBM) with non-defatted black soldier fly (Hermetia illucens) larvae meal (BSFLM) on growth, nutritional quality, haematology, biochemistry, oxidative stress biomarkers, and bacterial disease resistance in juvenile basa (Pangasius bocourti). Four isonitrogenous and isolipidic diets (30% crude protein) were formulated to replace 0% (T0), 40% (T40), 80% (T80) and 100% (T100) of crude protein from SBM with BSFLM. Fingerlings (initial weight = 18.00 ± 1.25 g, n = 135) were stocked in aquaria. At end of the growth experiment, fifteen fish from each group were exposed to bacterial (Staphylococcus aureus) challenge (0.80 CFU/ml) for fifteen days. Fish in BSFLM substituted groups showed significantly higher growth and condition as compared to those in T0. Inclusion of BSFLM did not affect the chemical composition, profile of amino acids, and fatty acids in fish. However, the levels of lauric acid, linoleic acid, α-linolenic acid, decosahexanoic acid, and eicosapentanoic acid increased in BSFLM fed fish. The concentration of catalase, superoxide dismutase and lysozyme increased with addition of BSFLM in diet at end of the growth experiment. These oxidative stress biomarkers indicate that replacement of SBM with BSFLM up to 80% improved the growth and disease resistance in basa against gram-positive bacteria. BSFLM can be a future alternative protein source for commercial production of aquafeed for this species.

Dietary replacement of soybean meal by fermented feedstuffs for aged laying hens: effects on laying performance, egg quality, nutrient digestibility, intestinal health, follicle development, and biological parameters in a long-term feeding period

This study aimed to investigate the effects of dietary supplementation with fermented soybean meal (FSM) or fermented miscellaneous meal (FMM, cottonseed meal: coconut meal=at a 1:1 ratio) on the intestinal health, laying performance, egg quality, and follicle development of laying hens. A total of 1,008 54-wk-old laying hens were randomly divided into 7 treatment groups and fed a corn-soybean base diet in addition to 2%, 4%, and 8% FSM or FMM. The results showed that fermentation increased the contents of crude protein, amino acids (Ser, Gly, Cys, Leu, Lys, His, and Arg), and organic acids (butyric acid, citric acid, succinic acid) and decreased the contents of neutral and acid detergent fiber in the soybean and miscellaneous meals (P < 0.05). Compared with the results found for the control group, feeding with 4% FSM increased the egg production, egg mass and average daily feed intake (ADFI), and feeding with 4% FMM increased the ADFI of laying hens (P < 0.05). Furthermore, feeding with 8% FMM reduced the productive performance and laying performance, supplementation with 4% FSM increased the eggshell strength and weight, and 2 to 4% FSM increased the egg albumen height and Haugh unit (P < 0.05). Moreover, 2 to 8% FSM or 2 to 4% FMM enhanced the apparent digestibility of dry matter, crude protein, and NDF for laying hens (P < 0.05). The relative weight, villus height, crypt depth, and villus:crypt ratio of the jejunum were higher in the 4% FSM- and FMM-fed groups (P < 0.05). Moreover, diamine oxidase (DAO) activity, transepithelial electrical resistance (TEER), and the expression of tight junction proteins (ZO-1, Occluding, and Claudin1), the intestinal stem cell marker Lgr5, and the proliferation cell marker proliferating cell nuclear antigen (PCNA) was upregulated in the jejunum of laying hens fed 4% FSM and FMM (P < 0.05). The relative weight of the ovaries, and the number of small yellow follicles and large white follicles were elevated after 4% FSM or FMM supplementation. Furthermore, the levels of serum follicle-stimulating hormone and luteinizing hormone were increased in the 4% FSM and FMM groups (P < 0.05). In conclusion, the supplementation of laying hen feed with FSM and FMM improved the laying performance, egg quality, intestinal barrier function, and follicle development of aged laying hens, and 4% FSM supplementation was optimal.

Dietary replacement of soybean meal with heat-treated soybean meal or high-protein corn distillers grains on nutrient digestibility and milk composition in mid-lactation cows.

Lactation diets dependent on rumen undegradable protein (RUP) sources derived from soybean meal (SBM) products are generally high in Lys and poor in Met. We conducted an experiment to evaluate the effects of increasing dietary RUP and altering digestible AA supply by inclusion of heat-treated soybean meal (HTSBM) or high-protein corn dried distillers grains with soluble (DDGS) on performance in mid-lactation dairy cows. Twenty-four Holstein cows (200 ± 40 d in milk and 30.0 ± 3.92 kg/d of milk yield) blocked according to parity, milk yield, and days in milk were used in a 3 × 3 Latin square design experiment with 21-d periods. Treatments were (1) control (CON), a diet with 6.0% RUP containing 15.9% SBM as the main protein source; (2) HTSBM, a diet with 6.7% RUP containing 4.4% HTSBM partially replacing SBM; and (3) high-protein DDGS (FP; FlexyPro, SJC Bioenergia), a diet with 6.9% RUP containing 5.34% FP partially replacing SBM and ground corn. Diets had similar crude protein (16.9%) and net energy of lactation. Data were submitted to ANOVA using the mixed procedure of SAS software (SAS Institute Inc.). Treatment differences were evaluated using orthogonal contrasts: (1) increasing RUP (SBM vs. HTSBM + FP) and (2) altering digestible AA supply (HTSBM vs. FP). Cows fed HTSBM and FP had greater intake (values in parentheses represent treatment means of CON, HTSBM, and FP, respectively) of neutral detergent fiber (7.14, 7.35, and 7.69 kg/d), crude protein (4.27, 4.37, and 4.51 kg/d), and ether extract (0.942, 0.968, and 1.04 kg/d) compared with cows fed CON. Feeding FP resulted in greater intake of neutral detergent fiber and ether extract compared with HTSBM. Cows fed HTSBM and FP had lower sorting index for feed particles <4 mm than cows fed CON (1.029, 1.008, and 1.022). Feeding FP resulted in greater intake of feed particles <4 mm compared with HTSBM. Treatments containing HTSBM or FP tended to decrease organic matter digestibility (72.4, 71.2, and 71.1%), but no other effects were detected in digestibility of neutral detergent fiber, crude protein, or ether extract. No evidence for differences among treatments was detected in excretion of purine derivatives in milk and urine. Milk yield was greater in cows fed HTSBM or FP than in cows fed CON (28.0, 28.9, and 28.8 kg/d, respectively). Cows fed HTSBM or FP tended to have greater energy-corrected milk and protein yield compared with those fed CON. Milk protein concentration was greater in DDGS cows than those in the HTSBM group (3.45 and 3.40%, respectively). No differences were detected in milk fat yield and concentration, milk urea nitrogen, feed efficiency, or serum concentrations of urea and glucose. Overall, increasing dietary RUP by feeding HTSBM or FP improved intake of nutrients and milk yield without affecting feed efficiency. Altering digestible AA supply while maintaining similar dietary RUP had negligible effects on performance of cows.

Effects of soybean meal replacement byHermetia illucens larvae meal on growth performance, meat quality and gastrointestinal health in broilers

The growing demand for broilers production is related to environmental and economic impacts worldwide, increasing the urgency to find alternative feed ingredients. Recent studies indicated the productive potential of some insect species, which may serve as a protein and lipid source for animal feeding, as the case of black soldier fly (BSF) larvae (Hermetia illucens). This study aimed at assessing the effects of dietary replacement of soybean meal by BSF larvae meal in broiler diets, in growth parameters, liver and intestinal tract morphology, carcass and meat quality. Forty-eight 1-day old chicks (Ross 308) were randomly assigned to one of four feeding regimes, which consisted of a basal diet in which soybean meal was replaced BSF larvae meal, at levels of 0% (Hi0), 25% (Hi25), 50% (Hi50) and 75% (Hi75). The animals’ excreta were collected and registered daily, and half the animals were slaughtered at 28 days of age. The liver, caecum and other portions of the intestines were analysed, and the intestinal tract was collected for histopathological analysis. BSF larvae meal did not affect carcass weight, but it resulted in a lower weight of the right breast of birds when fed with a 75% inclusion level (P=0.049). In addition, all growth parameters, general meat quality and excreta production were similar among treatments, while excreta characteristics were significantly different (P&lt;0.05). Dietary BSF meal resulted in lower liver weight (P=0.03) in comparison to the control. No histopathological lesions were found in the gastrointestinal tract and morphometric traits were similar among treatments. It was concluded that the dietary inclusion of BSF larvae meal for broilers did not significantly affect the animals’ growth and gut health. This alternative feed ingredient can be used as a more sustainable option for broilers at up to 75% of soybean meal replacement.

Effects of replacing soybean meal with linseed meal in broiler diet on selected broilers’ blood parameters, meat chemical composition, fatty acid profiles, and sensory characteristics

The study was conducted with the objective of determining the effects of the dietary replacement of soybean meal with graded levels of linseed meal on selected blood parameters, meat composition, fatty acid profiles, and meat quality of broiler chickens. Cobb500 broilers were fed diets containing linseed meal at 0% (T1), 6.5% (T2), 13% (T3), 19.5 (T4), and 26% (T5), replacing 0% to 100% soybean meal in compound rations for 45 days. The experiment was conducted using a completely randomized design with five treatments, each replicated three times with 12 birds. Blood hematological indices were not affected (P&amp;gt;0.05) by treatment diets while among the blood biochemistry triglyceride and cholesterol concentrations in T1 were higher (P&amp;lt;0.05) than in T3, T4, and T5. Glucose was higher in T3, T4, and T5 than in T1 and T2 (P&amp;lt;0.01). The breast and thigh proximate composition for crude protein (CP), ether extract (EE), ash, carbohydrate, and gross energy were similar (P&amp;gt;0.05) among treatments. The sensory scores for breast and thigh meat samples were not different (P&amp;gt;0.05) among treatments. The palmitic acid content of breast and thigh meat was higher (P&amp;lt;0.05) in T1 and T2 than in the other treatments. Eliadic and stearic acid concentrations in thigh meat were greater (P&amp;lt;0.05) in T1, T2, and T3 than in T4 and T5. The oleic and linoleic acid contents of thigh meat were higher (P&amp;lt;0.05) in T2 and T3 than in the rest of the treatments. Breast linolenic acid was high (P&amp;lt;0.05) in T5 but similar in thigh meat. Total saturated fatty acid (SFA) showed a decreasing trend with an increasing level of linseed meal (LSM) in the ration. The ratio of monounsaturated fatty acid (MUFA) to SFA for breast and thigh was higher in T4 and T5 than in T1 and T2. Breast meat ratio of omega-6 (n-6) to omega-3 (n-3) showed a decreasing trend as the level of LSM replacement for soybean meal (SBM) in the ration increased. Generally, linseed meal replacement levels up to 26% in the broilers’ diet improves the essential fatty acid content of chicken meat without affecting the proximate composition and the chickens’ normal blood indices and does not alter meat sensory attributes.

Impacts of dietary replacement of soybean meal with dried Ulva lactuca meal on growth performance, feed efficiency, and physiological responses of Oreochromis niloticus

Impacts of dietary replacement of soybean meal with dried Ulva lactuca meal on growth performance, feed efficiency, and physiological responses of Oreochromis niloticus

The effect of the dietary inclusion of pea seeds of colored-flowered and white-flowered varieties on gastrointestinal function in turkeys.

This study investigated the effects of dietary replacement of soybean meal (SBM) with graded levels of pea seeds (PS) on the gastrointestinal function of turkeys. Seeds of 2 pea varieties, a colored-flowered variety and a white-flowered variety (CFP and WFP, respectively) were fed to 56-d-old birds for 8 wk. A total of 539 female Hybrid turkeys were allocated to 7 groups, each group consisted of 7 pens with 11 birds per pen. The experiment had a 2-factorial design, with 3 dietary inclusion levels of PS (100, 200 and 300 g/kg) and 2 pea varieties (CFP and WFP). The control group (diets without PS) was compared with CFP and WFP treatments by simple contrast analysis. In comparison with CFP seeds, WFP seeds contained 7-fold less tannins (0.67 vs. 4.66 g/kg) and less non-starch polysaccharides (NSP, 117.8 vs. 132.7 g/kg), but more trypsin inhibitors (1.34 vs. 0.98 g/kg) and starch (489 vs. 455 g/kg). A rise in the PS content of diets from 100 to 200 and 300 g/kg increased the weight of the small intestine (P = 0.031) and the dry matter (DM) content of intestinal digesta (P = 0.001), but it had no effect on the pH of digesta. Only the highest PS content differentiated the concentrations of short-chain fatty acids (SCFAs) in the small intestinal digesta (WFP > CFP, P = 0.008), whereas PS did not cause any changes in the morphological parameters of the small intestinal mucosa. The dietary inclusion of PS had no influence on the levels of acetate, butyrate, putrefactive SCFAs or total SCFAs in the cecal contents. Apart from increasing the activities of β-glucosidase (P = 0.017) and β-galactosidase (P = 0.025), pea varieties did not affect the activities of the analyzed cecal microbial enzymes. However, CFP seeds decreased the DM content (P = 0.041) and increased the pH of cecal digesta, compared with WFP seeds (P = 0.013). The results of this study, pointing to a few differences in the functional parameters of the small intestine and cecum, indicate that tannins are not a factor differentiating the suitability of CFP and WFP seeds in the nutrition of finisher turkeys. The inclusion of PS at 200 and 300 g/kg of the diet reduces the content of SBM and wheat in turkey diets, which has a positive effect on gastrointestinal function.

Effects of Dietary Replacement of Soybean Meal with Corn Distillers Dried Grain with Solubles on Growth Performance and Carcass Characteristics of Broilers

Effects of Dietary Replacement of Soybean Meal with Corn Distillers Dried Grain with Solubles on Growth Performance and Carcass Characteristics of Broilers

Effect of dietary cowpea (Vigna unguiculata [L] walp) and chickpea (Cicer arietinum L.) seeds on growth performance, blood parameters and breast meat fatty acids in broiler chickens

The effects of dietary replacement of soybean meal (SBM) with two different protein sources (cowpea [CWP] and chickpea [CKP]) on growth performance (GP), blood parameters and breast meat fatty acids (FA) content were evaluated. A total of 780 one-d-old broiler chicks (Cobb 500) were allocated, over 2 growth phases (starter, 0–21 d; finisher, 22–42 d), to 5 dietary treatments as follow: (1) a corn-SBM basal diet as control; (2) 10% raw cowpea; (3) 20% raw cowpea; (4) 10% raw chickpea and (5) 20% raw chickpea. The results indicated that CWP and CKP diets conducted to similar GP (body weight, body weight gain, feed intake and feed conversion ratio) when compared to the SBM diet, but changes in the content of blood parameters were observed. Glucose and total cholesterol decreased significantly in birds fed CWP (p<.0001) and CKP (p<.001) diet. Also, the use of CWP and CKP had a significant effect on the content of breast muscle FA, especially for alfa-linolenic (p <.0001), eicosapentaenoic (p<.0001), docosapentaenoic (p=.034) and docosahexaenoic (p =.003), which were higher than those fed SBM diet. In addition, there was a significant interaction between the protein source and the inclusion level (S × L) for the majority of the FA’s. Based on the results obtained, it can be concluded that CWP and CKP seeds represent an interesting alternative protein sources, which can improve broilers performance, health status and breast fatty acids composition. Highlights Cowpea (CWP) and chickpea (CKP) can be used as replacement for soybean meal in broiler chickens diets, at inclusion levels up to 200 g/kg. Dietary use of CWP and CKP decreased plasma cholesterol and glucose concentration. Use of CWP and CKP in broilers diets positively modified breast meat fatty acids, especially α-linolenic acid, eicosapentaenoic or docosahexaenoic acids with potential benefits to human nutrition.

Effect of dietary replacement of soybean meal with linseed meal on feed intake, growth performance and carcass quality of broilers

To increase the chicken's productivity and performance it is imperative to exploit underutilized oil crops such as linseed meal as protein source. This study evaluated the effect of replacing soybean meal with graded levels of linseed meal on feed intake, growth performance and carcass parameters of broiler chickens. A total of 180 day-old Cobb500 broilers were distributed to five treatment diets in a completely randomized design replicated three times with 12 chicks each. Isocaloric and isonitrogenous treatment diets formulated were T1 (0%, diet with no linseed meal), T2 (25%), T3 (50%), T4 (75%) and T5 (100%, soybean meal in the diet was replaced by linseed meal). The feeding experiment lasted for 44 days. The total feed intake, mortality rate and feed conversion ratio (FCR) during starter phase were similar (P > 0.05) among treatments. High (P < 0.05) starter phase body weight was recorded for T3 compared to T1, T2, T4 and T5 treatments but T2, T4 and T5 had similar average daily gain. The feed intake, body weight change, FCR and mortality during finisher phase and entire period were similar (P > 0.05) among treatment groups. The weight of most carcasses were similar (P > 0.05) among treatment except the weight of kidney, heart, breast, liver and abdominal fat. Kidney weight for T1 and T3 were higher (P < 0.05) than for T2, T4 and T5. The weight of heart for T3 was higher than T2 and T5 while T1, T3 and T4 were similar (P > 0.05). High (P < 0.05) breast weight were observed for T3 than T2 and T4. Liver weight for T3 was greater (P < 0.05) than T2 and T5. The total feed cost decreased with increasing levels of linseed meal. High net return was obtained from T3 followed by T4 and T2. The results showed that although linseed meal can replace 100% soybean meal in the ration without detrimental effect on the health, replacement at 50% (T3) is recommended for better performance of broilers.

The effects of the dietary replacement of soybean meal with yellow mealworm larvae (Tenebrio molitor) on the growth, nutrient digestibility and nitrogen output of fattening rabbits

The present study aimed to obtain novel information concerning the effects of the total dietary replacement of soybean meal (SBM) with yellow mealworm (Tenebrio molitor; TM) on the coefficients of total tract apparent digestibility (CTTAD) in the diet and on the nitrogen output of fattening rabbits. Two diets were formulated that included 60 g/kg SBM (SBM diet) or 30 g/kg TM (TM diet) as the main crude protein (CP) source. The TM diet had slightly lower CP content than the SBM diet (141 vs. 150 g/kg). A total of 30 rabbits (Hyplus PS 19 x Hyplus PS 40; males) weaned at the age of 32 day were randomly allocated into 2 groups (15 rabbits per group), individually housed, and fed one of the two diets ad libitum for 47 days. Feed intake was measured daily during the entire fattening period (between 32 and 79 day of age). The total faecal and urine output were individually measured between 57 and 61 day of age. A lower daily weight gain (ADWG) between 32 and 53 day of age (by 6.9 g/day, 12 %; P = 0.034), resulting in lower live weight (LW) at the age of 53 day, and a lower feed intake (ADFI) between 32 and 79 day of age (by 13 g/day, 7 %; P = 0.047) were recorded in rabbits fed the TM diet compared to rabbits fed the SBM diet. The ADWG or feed conversion ratio concerning the entire fattening period, the final LW of rabbits, or the CTTAD of the diets were not affected by treatments. Whatever the diet, on average, losses of nitrogen in faeces represented 26 % of the nitrogen intake. Lower losses of nitrogen in urine (by 0.35 g/day, 32 %; P = 0.007) and total excretion of nitrogen (by 0.53 g/day, 23 %; P = 0.003), as well as a higher nitrogen retention coefficient (by 0.06, 11 %; P = 0.026) were detected in rabbits fed the TM diet compared to rabbits fed the SBM diet. It can be concluded that fattening rabbits effectively utilized the diet containing TM without detrimental effects on the CTTAD of the diets or on their nitrogen output.

Dietary replacement of soybean meal with lipid matrix-encapsulated urea does not modify milk production and composition in dairy goats

This study evaluated the performance and quality of milk from dairy goats supplemented with urea protected into lipids matrix microencapsulation for controlled release replacing soybean meal. Eight crossbred Alpine dairy goats, multiparous, with an average lactation stage of 70 ± 5.0 days after calving were distributed in a double latin square design was used with four treatments [SBM = control, soybean meal as nitrogen (N) source; microencapasulated urea using beeswax (BWUME), carnauba wax (CWUME) and vegetable fat (VFUME) as encapsulants], replacing soybean meal at 25 % in goat diets. The BWUME replacing SBM protein improved (P ≤ 0.05) intake of CP, N-total, nitrogen insoluble in acid detergent (NIAD), and EE also improved milk production4% corrected, solids-corrected milk, protein, fat, total solids and defatted dry extract and feeding conversion comparing other microencapsulated systems. BWUME and CWUME presented greater (P ≤ 0.05) crude ash, nitrogen insoluble in neutral detergent (NIND) and NDFap intakes compared to VFUME and SBM. The CWUME and VFUME presented greater and lower DMI and crude energy intakes, respectively comparing to other microencapsulated systems and SBM. The TC and NFC intake were greater (P ≤ 0.05) in SBM and VFUME, and lower for BWUME. The SBM presented greater milk production, lower fat and total solids contents compared to other microencapsulated systems. Hematological parameters and serum protein in goats did not differ among treatments. However, at ½ hour before and 1:30 h (P = 0.023) after feeding, the goats receiving BWUME presented greater (P ≤ 0.05) blood urea nitrogen (BUN) comparing to other diets. At 3:00 h after feeding, the BUN concentrations of the all microencapsulated urea formulations were higher que SBM (P = 0.011). The BWUME inclusion for controlled release replacing 25 % SBM promoted similar milk production, solids content and feeding conversion, as well as greater CP intake, N-intake and fat milk concentration in dairy goats.

Effects of dietary replacement of soybean meal with dried distillers grains with solubles on the microbiota occupying different ecological niches in the rumen of growing Hu lambs

BackgroundDiet has a profound impact on the rumen microbiota, and the impact can vary among the different rumen ecological niches (REN). This study investigated the effects of dietary replacement of soybean meal (SBM) with dried distillers grains with solubles (DDGS) on the rumen microbiota occupying different REN of growing Hu lambs. After a 9-week feeding trial, 6 lambs from each dietary treatment (SBM vs. DDGS-based diets) were slaughtered for sample collection. The microbiota of the rumen solid, liquid, and epithelium fractions was examined using amplicon sequencing analysis of bacterial 16S rRNA gene, functional prediction, and qPCR.ResultsNo interaction of dietary protein source (PS) and REN were detected for virtually all the measurements made in this study. The DDGS substitution resulted in very limited influence on bacterial community structure. However, the metabolic pathways predicted from 16S rRNA gene sequences varied greatly between SBM- and DDGS-based diets. The populations of rumen total bacteria, fungi, sulfate-reducing bacteria (SRB), and methanogens were not influenced by DDGS substitution, but the population of protozoa was reduced. The bacterial communities in rumen solid (RS) and liquid (RL) were similar in taxonomic composition but were different in relative abundance of some taxa. In contrast, the bacterial composition and relative abundance of rumen epithelium (RE) were greatly distinct from those of the RS and the RL. In alignment with the bacterial relative abundance, the metabolic pathways predicted from 16S rRNA genes also varied greatly among the different REN. The populations of total bacteria, protozoa, and methanogens attached to the RE were smaller than those in the RS and RL, and the fungal population on the rumen epithelium was smaller than that in the RS but similar to that in the RL. On the contrary, the SRB population on the RE was greater than that in the RS and RL.ConclusionsSubstitution of SBM with DDGS had greater impact to the protozoa than to the other microbes, and the microbial community structure and functions at different REN are distinct and niche-adapted.

DIETARY REPLACEMENT OF SOYBEAN MEAL BY TOASTED SUNFLOWER SEEDMEAL IN THE DIET OF Clarias gariepinus: EFFECT ON GROWTH, BODY COMPOSITION, DIGESTIBILITY, HAEMATOLOGY AND HISTOPATHOLOGY OF THE LIVER.

Dietary replacement of soybean meal by toasted sunflower meal in Clarias gariepinus diet was investigated in a 56-day feeding trial using growth, body composition, digestibility, haematology and liver histology as indices of assessment. Sunflower seed meal was toasted at 2040C for 5, 10 and 15 minutes. Control diet consist of soybean meal which was replaced by differently toasted sunflower seed meal at a rate 15, 30 and 45% to produce nine 40% crude protein, 18kj/kg test diets. Triplicate groups of fish in 70-litre capacity aerated rectangular plastic tanks were assigned to each dietary treatment at a stocking rate of 15 fingerlings (4.97±0.42g average weight) per tank in a completely randomized design. Fish were fed to satiation. Data generated from the experiment were subjected to one-way analysis of variance. The results of the experiment revealed that up to 30% replacement level of the differently toasted sunflower had a statistically similar results with soybean-based control diets using growth, body composition, digestibility and haematology as indices. Liver histology however show that massive fatty infiltration recorded in the liver of fish fed TSF145 and TSF 345 and diffuse vacuolar degeneration of hepatocytes and kupffer cell hyperplasia observed in the liver of fish fed TSF245 and TSF330.

The influence of dietary replacement of soybean meal with high-tannin faba beans on gut-bone axis and metabolic response in broiler chickens

Abstract Faba bean (FB) seeds can be a good protein-energy component in animal feed. However, the presence of anti-nutritional substances is a negative feature of FB seeds. The aim of this study was to examine the influence of different levels of unprocessed FB seeds in feed on the gut-bone axis and metabolic profile in broilers. Ninety six, 1-day-old Ross 308 broiler chickens were randomly selected to one of the 3 dietary treatments (32 chickens in each, divided into 8 pens with 4 birds per each pen): the control group fed standard diet with soybean meal and without FB seeds, group I fed 8/15% (starter/grower) of high-tannin FB seeds, and group II fed 16/22% of high-tannin FB seeds. Bone mechanical examination, hematological and serum biochemical analysis as well histomorphometry of small intestine and liver tissue were performed. The intake of high-tannin FB seeds, irrespective of their amount, did not alter the bone geometric, mechanical and densitometric parameters nor influenced basal hematological parameters, however it resulted in: decreased serum concentration of total cholesterol and calcium; a reduced longitudinal myenteron of small intestine; increased mucosa and villus epithelium thickness, villus length, thickness and absorptive surface in duodenum; increased number of active crypts in jejunum; unchanged collagen area, intercellular space, and total cell number in the liver; decreased number of multinuclear hepatocyte cells. Moreover, the livers of birds fed the higher dose of high-tannin FB seeds had lymphocytic infiltrates in portal tracts and sinusoids. Feeding of unprocessed high-tannin FB seeds exerted an influence on the gastrointestinal tract by increased absorptive surface. In conclusion, the dietary inclusion of unprocessed high-tannin FB seeds had no negative effects on broiler growth, tibial bone mechanical properties and intestinal characteristics. Unprocessed high-tannin FB seeds may be used in broiler diets, but their dietary levels should not be higher than those discussed.

Effects of dietary inclusion of high- and low-tannin faba bean (Vicia faba L.) seeds on microbiota, histology and fermentation processes of the gastrointestinal tract in finisher turkeys

This study evaluated the effects of dietary replacement of soybean meal (SBM) with graded levels of faba bean (FB) seeds with high or low tannin content (HT or LT) on the gastrointestinal function and growth performance of turkeys at 13–18 weeks of age. Hybrid Converter turkeys were distributed into 7 treatments corresponding to 7 different finisher diets: a control wheat-soybean meal-based (FB0) diet and experimental diets where SBM was partially replaced with HT or LT seeds at 100, 200 and 300 g/kg. Each treatment comprised 210 turkeys, with seven replicate pens and 30 birds per pen. The LT treatment decreased jejunal crypt depth (vs. FB0; P = 0.049) and the experimental factors had no significant effect on the analysed caecal histological parameters. In comparison with the FB0 diet, diets containing HT and LT FB contributed to an increase in the total bacterial counts (P = 0.001 and P = 0.033) and Bacteria domain (P = 0.001 and P = 0.060), and a decrease in the counts of Bacteroides (P = 0.002 and P = 0.013). Diets containing LT FB reduced the abundance of Salmonella bacteria, relative to the FB0 diet (P = 0.011) and diets with HT FB (P = 0.023). The LT treatment decreased the counts of total bacteria and Bacteria domain (P = 0.005), in comparison with the HT treatment. The highest ileal short-chain fatty acid (SCFA) concentrations were observed in response to the LT200 diet. LT diets stimulated increased SCFA production in the caeca, relative to the FB0 diet (P = 0.022), and the opposite effect was noted when HT and SBM dietary treatments were compared. In comparison with HT diets, LT diets led to a desirable increase in the concentrations of all major fatty acids (acetic, propionic and butyric) in the caecal contents. It can be concluded that FB seeds enhanced fermentation processes in the gastrointestinal tract of turkeys. In comparison with HT FB, LT seeds improved selected parameters of intestinal function, including a decrease in the counts of Salmonella bacteria (P = 0.023), increased SCFA production (including butyrate; P = 0.001), and a decrease in the pH of intestinal digesta (P = 0.105). In conclusion, both LT and HT FB seeds, the latter containing up to 7.1 g/kg tannins, can be included in finisher turkey diets at up to 300 g/kg as a safe and effective substitute for SBM.

Gut-bone axis response to dietary replacement of soybean meal with raw low-tannin faba bean seeds in broiler chickens.

It seems that faba bean (FB) seeds could be a good protein-energy component in animal feed, but the presence of anti-nutritional substances limits their use as a substitute of soybean meal. The aim of the study was to examine the influence of different concentrations of raw, low-tannin, FB seeds on the gut-bone axis in Ross 308 broilers. One-day old chickens were randomly subjected to one of the 3 dietary treatments: the control group was fed standard diet based on soybean meal and without FB seeds, and two groups were fed 8%/15% and 16%/22% of raw low-tannin FB seeds in the starter and grower, respectively. On the 35th day, hematological and serum biochemical analyses as well histomorphometry of the small intestine and liver tissue and bone mechanical tests were performed. The diet type had no effect on the body weight gain and feed conversion ratio. However, the basal intestinal structures were significantly reduced in birds fed the lower concentration of FB. The enlargement of nerve plexuses was dependent on the concentration used in the diet and, additionally, on the kind of plexus and location in the intestinal tract. The liver was characterized by an increase in non-hepatocytes. There was no influence of the low-tannin FB seeds on most of the analyzed serum parameters in the 35-day-old broiler chickens, except the decreased concentration of total cholesterol and Ca in both experimental groups, triglycerides in group I, and P and uric acid in group II. Furthermore, the increasing concentration of the dietary low-tannin FB did not influence the activities of AspAT (except the group fed the higher amount of FB), ALAT, and LDH. The broiler chickens had no visible leg lesions and no problem in the locomotor function, but the tibiae were lighter mainly in birds fed the higher concentration of FB seeds. Geometric analysis revealed reduction of the cross section area and wall thickness, indicating a decline in the bone midshaft, which influenced the densitometric parameters and the results of mechanical tests. In conclusion, the dietary inclusion of raw low-tannin FB seeds had no negative effects on broiler growth, but disturbed the intestine structure and tibia characteristics. Therefore, all these negative effects necessitate additional examinations before inclusion of raw low-tannin FB seed into poultry diet.