Dietary peNDF Research Articles

Effect of dietary peNDF levels on digestibility and rumen fermentation, and microbial community in growing goats.

Physically effective neutral detergent fiber (peNDF) is a concept that accounts for the particle length of NDF in diets, sustaining the normal chewing behavior and rumen fermentation of ruminants. Specifically, peNDF>1.18 is the commonest one that is calculated from NDF and the percentage of feed dry matter left on the 1.18, 8.00, and 19.00 mm sieves. This study aimed to investigate the effects of different levels of peNDF>1.18 on the rumen microbiome and its correlation with nutrient digestibility and rumen fermentation in goats. A total of 30 Lezhi black goats were randomized and blocked to five dietary treatments (n = 6). All the diets were identical in composition but varied in hay lengths, leading to the different peNDF>1.18 content of the diets: 32.97, 29.93, 28.14, 26.48, and 24.75%. The results revealed that the nutrient digestibility increased when dietary peNDF>1.18 levels decreased from 32.97% to 28.14%, with the highest digestibility at 28.14% peNDF>1.18 treatment, after which nutrient digestibility decreased with the decreasing of dietary peNDF levels. Ruminal NH3-N concentrations in the 29.93% and 28.14% groups were higher than that in the 24.75% group (p < 0.05). Ruminal microbial protein concentration was the highest in the 32.97% group (p < 0.05). Daily CH4 production in the 32.97% and 24.75% peNDF>1.18 treatments was lower than that in the 26.48% group (p < 0.05) and no differences were observed among other groups. The relative abundance of rumen fungi at the phylum and genus levels and archaea at the species were affected by dietary peNDF>1.18 content. In conclusion, decreasing dietary peNDF>1.18 levels within a certain range can improve nutrient digestibility and change the rumen microbial community structure of goats. Dietary peNDF>1.18 level should be 28.14% (roughage length around 1 cm) among the five levels for 4 months Lezhi black goats with the purpose of optimal nutrient digestibility.

Changes in Rumen Bacterial Community Induced by the Dietary Physically Effective Neutral Detergent Fiber Levels in Goat Diets.

Physically effective neutral detergent fiber (peNDF) is a concept that accounts for the particle length of NDF in a feed, sustaining the normal chewing behavior and rumen fermentation of ruminants. This study aimed to elucidate the effects of dietary peNDF on growth performance and bacterial communities in the rumen of goats through a high-throughput sequencing technique. A total of 30 male Lezhi black goats were randomly assigned to five groups, corresponding to five diets with identical compositions and nutrient levels but with varying forage lengths (the peNDF1.18 contents of the diets were 33.0, 29.9, 28.1, 26.5, and 24.8%, respectively). The whole trial lasted for 44 days. As results show, feed intake and average daily gain were highest when peNDF1.18 content was 26.5%, in which the papilla length of the dorsal sac in rumen was the highest. Chao1 and ACE indexes were similar among the treatments, while Shannon and Simpson indexes of the peNDF1.18 = 28.1% group were the highest (p < 0.05). As the level of dietary peNDF1.18 decreased, the dominant phylum transitioned from Bacteroidetes to Firmicutes. The top three dominant genera of rumen bacteria were Prevotella 1, Ruminococcaceae NK4A214 group, and Christensenellaceae R-7 group. They all showed a quadratic correlation with dietary peNDF1.18 level (p < 0.05). The relative abundance of Ruminococcaceae UCG-011 was positively correlated, while that of Prevotella 1 was negatively correlated, with amino acid metabolism and energy metabolism (p < 0.01). In conclusion, dietary peNDF level influenced goat growth performance, rumen development, and rumen bacterial community structures, and a peNDF1.18 level between 26.5 and 28.1% was considered optimal for goat diet.

Feeding diets varying in forage proportion and particle length to lactating dairy cows: I. Effects on ruminal pH and fermentation, microbial protein synthesis, digestibility, and milk production

Physically effective neutral detergent fiber (peNDF) content of dairy cow diets was modified by varying the theoretical chop length of alfalfa silage and forage:concentrate (F:C) ratio, and effects on nutrient intakes, ruminal fermentation, site and extent of digestion, microbial protein synthesis, and milk production were evaluated. Estimates of dietary peNDF contents were compared with recommendations, and predictions of ruminal pH from peNDF and the recently developed physically adjusted neutral detergent fiber (paNDF) system were compared with observed pH. The experiment was designed as a triple 4 × 4 Latin square using 12 mid-lactating dairy cows with 4 intact, 4 ruminally cannulated, and 4 ruminally and duodenally cannulated cows. Site and extent of digestion and microbial protein synthesis were measured in a single 4 × 4 Latin square. Treatments were a 2 × 2 factorial arrangement; 2 forage particle lengths (FPL) of alfalfa silage (short and long) were combined with low (35:65) and high (60:40) F:C ratios [dry matter (DM) basis]. The peNDF contents were determined by multiplying the proportion (DM basis) of total mixed ration retained on 2 (8 and 19 mm; peNDF8.0) or 3 (1.18, 8, and 19 mm; peNDF1.18) sieves of the Penn State Particle Separator by the neutral detergent fiber content of the diet. The dietary peNDF contents ranged from 10.7 to 17.5% for peNDF8.0 or from 23.1 to 28.2% for peNDF1.18. Interactions between F:C ratio and FPL content were few. Increasing peNDF content of diets by increasing F:C ratio decreased DM intake, milk yield, and milk protein yield, whereas apparent total-tract DM digestibility and milk efficiency improved. Increasing F:C ratio improved ruminal pH status but decreased total volatile fatty acid concentration and microbial protein synthesis. Increasing peNDF content of diets via dietary FPL increased mean ruminal pH, but did not affect DM intake, total-tract digestibility, or milk production. The results indicate that feeding dairy cows a low F:C diet helps increase DM intake, milk production, and microbial protein synthesis, but may adversely affect feed digestibility and milk efficiency due to increased risk of subacute ruminal acidosis. Increased FPL improved ruminal pH status, but had minimal effects on feed intake, ruminal fermentation, nutrient digestibility, and milk production. The results indicate a trade-off between reducing the risk of subacute ruminal acidosis and maximizing ruminal fermentation, feed digestibility, and milk production of dairy cows. The paNDF model showed improvement in the predictability of ruminal pH over the peNDF model, but the accuracy of predictions varied depending upon the diet and ruminal fermentation variables considered in the equations.

Dietary protein level and corn processing method: Intake, digestibility, and feeding behavior of lactating dairy cows✰

The effects of corn processing and dietary protein level and their interaction on intake, digestibility, and feeding behavior in dairy cow was examined using eight multiparous Holstein dairy cows in mid-lactation (105 ± 9 days in milk and 47.2 ± 3 kg/day milk production at the start of the experiment) that were placed in a replicated 4 × 4 Latin square with a 2 × 2 factorial arrangement of treatments. Experimental diets contained either finely ground corn (FGC) or steam-flaked corn (SFC) based on either low protein (LP, 14.8%) or high protein (HP, 16.2%) content. Diets contained 40% forage (corn silage and alfalfa hay) and 40% corn grain. Dry matter intake did not differ between HP and LP diets. In contrast, cows fed LP had greater total tract digestibility compared with that for HP-fed cows. Cows fed LP had greater chewing time and lower chewing rate (g of DM/min) compared to those for cows fed HP. Cows receiving SFC had a lower intake of nutrients and tended to have greater ADF and starch digestibility than cows fed FGC. There was an interaction between corn processing and level of protein with respect to the apparent total tract digestibility with cows fed FGC with HP resulted in the lowest digestibility. Steam-flaked corn had lower density and greater moisture and mean particle size than the FGC. In comparison with FGC, SFC did not pass through the 8-mm sieve and caused significant increase in physical effectiveness factor (pef) and physically effective fiber (peNDF) in SFC diets compared to that for FGC diets. Cows fed SFC sorted against longer particles (P = 0.04) to a greater extent than cows fed FGC. Dietary protein level had no detectable effect on sorting index. Chewing time did not differ for cows fed SFC or FGC; however, cows fed SFC had lower chewing and rumination rate (g of DM/min). Chewing time per kg of DM and CP were greater and per kg of peNDF were lower in cows fed SFC rather than cows fed FGC. Results indicated that corn processing and dietary protein level may interact to affect nutrient digestibility, but did not affect the feeding and chewing behavior of lactating cows under our experimental conditions. Results also showed that dietary peNDF content and chewing activity can be effectively manipulated by steam flaking of corn, and cows fed SFC had greater peNDF and energy intake despite lower DMI.

Effects of physically effective neutral detergent fiber content on dry matter intake, digestibility, and chewing activity in Korean native goats (Capra hircus coreanae) fed with total mixed ration.

ObjectiveThis experiment was to determine proper physical traits in the diet for goats by investigating the effects of physically effective neutral detergent fiber (peNDF) content on dry matter intake (DMI), digestibility, and chewing activity in black goats fed with total mixed ration (TMR).MethodsSix growing wethers of Korean native black goats (Capra hircus coreanae) aged 8 months and weighing between 26.9 kg and 27.1 kg (27.03±5.05 kg) were used in this experiment. Three diets of varying peNDF content were obtained by original TMR (T1), 12,000 rpm grinding (T2), and 15,500 rpm grinding (T3) of the same TMR diet. The peNDF1.18 content of the experimental diets was 23.85%, 21.71%, and 16.22% for T1, T2, and T3, respectively.ResultsAverage daily gain (ADG) was higher in T2 group compared to those of the control and T3 groups, but ADG and DMI were not affected by the dietary particle size and peNDF content. Also, there was no difference between apparent nutrient digestibility of dry matter, crude fiber, ether extract, neutral detergent fiber, and acid detergent fiber. Although there was no significant difference, rumination and total chewing time were associated with decreased peNDF content.ConclusionThe feeding of peNDF-based TMR showed no impact on apparent nutrient digestibility and nitrogen balance. Further studies are required with a wider range of dietary peNDF level and particle size to better identify the effect of dietary peNDF and particle size on chewing activity and performance in goats.

Effects of corn straw or mixed forage diet on rumen fermentation parameters of lactating cows using a wireless data logger.

The objective of this study was to evaluate the effect of two different forage types on rumen fermentation parameters and profiles using a wireless data logger. Eight lactating cows were randomly assigned to one of two dietary treatments with a low forage diet with corn straw (CS) or a high forage diet with mixed forage (MF) as the forage source, respectively. Dietary physically effective neutral detergent fiber (peNDF) content was 11.3% greater in CS. Dry matter intake and milk fatty acid content decreased upon CS (P < 0.05). Ruminal pH, temperature and oxidation reduction potential (ORP) were monitored for 14 weeks. The CS group had significantly higher pH but lower temperature and ORP compared to MF (P < 0.01). With the CS diet regime, pH at the time before morning feeding, rumination and post-ingestion were significantly higher than those in the MF group (P < 0.05). However, times with the ruminal pH below 6.0 and 5.8 were significantly reduced (P < 0.05), whereas ruminal pH below 5.6 tended to be lower (P = 0.07). The results indicated that rumen fermentation parameters were affected by forage types and dietary peNDF content might be predominant in ruminal pH regulation.

Effect of dietary physically effective fiber on ruminal fermentation and the fatty acid profile of milk in dairy goats

The objective of this experiment was to characterize the relationship among rumen fermentation variables, milk fatty acid profile, and dietary physically effective neutral detergent fiber (peNDF) content in a study that controlled for the potential confounding effects of dissimilar dry matter intake among treatments. Ten multiparous Xinong Saanen dairy goats were divided into 2 groups with 2 ruminally cannulated goats per group. Goats in each group were assigned to 1 of 2 dietary treatments (high and low peNDF) according to a 2×2 crossover design with 2 periods. The peNDF content of alfalfa hay (proportion of neutral detergent fiber retained on an 8.0-mm screen) was 42.1% for the high-peNDF and 14.5% for the low-peNDF group. To ensure similar dry matter intake, each morning the amount of alfalfa hay consumed on the prior day by the high-peNDF group was determined (amount offered minus morning refusals), and this was the amount of hay offered to the low-peNDF group that day. Each adaptation period consisted of 21d, followed by a 9-d sampling period. Dry matter intake and milk production and composition were similar between treatments. Milk energy efficiency increased with low dietary peNDF. Duration of pH below 5.60 was longer for goats fed the low-peNDF ration compared with the high-peNDF ration (4.08 vs. 0.41h/d); however, mean rumen pH (6.05 vs. 6.13) was not different between treatments. Reducing dietary peNDF increased rumen total volatile fatty acids (114.6 vs. 95.1mM) and decreased chewing time (404 vs. 673min/d), but did not affect the ratios of acetate, propionate, and butyrate. The relative abundance of Fibrobacter succinogenes and Ruminococcus flavefaciens increased with reduced dietary peNDF, but Ruminococcus albus proportions were not influenced by treatment. Reducing dietary peNDF decreased the proportion of iso C14:0, iso C15:0, and trans-11 C18:1 in milk fat, whereas the iso C17:0 and trans-10 C18:1 increased. This study demonstrated that low dietary peNDF in dairy goats increases rumen volatile fatty acids, reduces chewing time, and is correlated to the amount of F. succinogenes and R. flavefaciens.

Effects of alfalfa hay particle size in high-concentrate diets supplemented with unsaturated fat: Chewing behavior, total-tract digestibility, and milk production of dairy cows

This study evaluated the effects of increasing the physically effective neutral detergent fiber (peNDF) intake of lactating dairy cows fed high-concentrate diets supplemented with unsaturated fat on intake, eating behavior, diet sorting, chewing activity, total-tract digestibility, and milk production and composition. Diets contained 24% alfalfa hay (AH), 16% corn silage, 58% concentrate, and 2% yellow grease [dry matter (DM) basis], and dietary peNDF content was increased by varying the particle size (PS) of the AH. Nine multiparous cows averaging 87.8±14.8d in milk and weighing 653±53kg were randomly assigned to a triplicate 3×3 Latin square. During each 21-d period, cows were offered 1 of 3 total mixed rations that varied in PS of AH: fine, medium, and long, with a geometric mean particle length of 3.00, 3.57 and 3.87mm, respectively. Increasing PS quadratically affected DM intake (DMI; 24.7, 25.4, and 23.7kg/d, for fine, medium, and long, respectively), but cumulative DMI at 2, 4, and 6h after feeding was similar across treatments, averaging 23.4, 35.6 and 46.4% of total DMI for the 3 time points, respectively. Increased peNDF intake did not affect feed sorting, but increased daily eating time, and eating and total chewing time per kilogram of DMI. Daily rumination time exhibited a quadratic response, with highest rumination time for the medium diet. Dietary PS had no effects on digestibility in the total tract, but we observed, for fine, medium, and long diets, quadratic responses in milk production (41.5, 43.3, and 40.4kg/d), 4% fat-corrected milk production, and milk protein yield. Milk fat content decreased linearly with increasing PS, but milk fat content and fat:protein ratio were low for all treatments, likely due to adding unsaturated fat to a diet containing a high level of nonfiber carbohydrates (42.2% of DM). The composition, degree of saturation, and total conjugated linoleic acid content of fatty acids in milk fat were not affected by the change in peNDF content of the diet. The study indicates that a moderate increase in the PS of AH in diets containing unsaturated fat elevates peNDF intake and increases chewing activity, DMI, milk yield and milk fat production. However, the effects of dietary PS were quadratic, with maximum DMI and milk production observed with diets supplying 24% dietary peNDF (measured as the proportion of the ration retained on sieves >1.18mm multiplied by dietary neutral detergent fiber content; DM basis).

Effects of physically effective fiber on chewing activity, ruminal fermentation, and digestibility in goats1

The objective of this study was to evaluate the effects of physically effective NDF (peNDF) in goat diets containing alfalfa hay as the sole forage source on feed intake, chewing activity, ruminal fermentation, and nutrient digestibility. Four rumen-fistulated goats were fed different proportions of chopped and ground alfalfa hay in a 4 × 4 Latin square design. Diets were chemically similar but varied in peNDF content: low, moderate low, moderate high, and high. Dietary peNDF content was determined using the Penn State Particle Separator with 2 sieves (8 and 19 mm) or 3 sieves (1.18, 8, and 19 mm). The dietary peNDF content ranged from 1.9 to 11.7% using the 2 sieves and from 15.2 to 20.0% using the 3 sieves. Increasing forage particle length increased intake of peNDF, but decreased DMI linearly (P = 0.05). Ruminating and total chewing time (min/d) were increased linearly (P = 0.001 and 0.007, respectively) with increased dietary peNDF, resulting in a linear reduction (P < 0.001) in the duration of time that ruminal pH was less than 5.8 (10.9, 9.0, 1.2, and 0.3 h/d, respectively). Increasing dietary peNDF tended to increase the molar proportion of propionate linearly (P = 0.08) and decrease the molar proportion of butyrate (P = 0.09), but did not affect total VFA concentration. Increasing dietary peNDF linearly decreased the apparent digestibility of OM, NDF, and ADF in the total tract (P = 0.009, 0.003, and 0.008, respectively). This study demonstrated that increasing the dietary peNDF contained in alfalfa hay forage stimulated chewing activity and improved ruminal pH status, but reduced nutrient intake and efficiency of feed use.

Effects of rice straw particle size on digesta particle size distribution, nitrogen metabolism, blood biochemical parameters, microbial amino acid composition and intestinal amino acid digestibility in goats

Effects of rice straw particle size and physically effective neutral detergent fiber (peNDF) on particle size distribution of different digestive tract, nitrogen (N) metabolism, blood biochemical parameters, microbial amino acid (AA) composition and intestinal AA digestibility in goats were investigated. A 4 × 4 Latin square design was employed using four mature Liuyang black goats fitted with permanent ruminal, duodenal, and terminal ileal fistulae. During each of the four periods, goats were offered one of four diets that were similar in chemical composition, but varied in particle sizes and peNDF through alteration of the theoretical cut length of rice straw (10, 20, 40 and 80 mm, respectively). Dietary peNDF contents of four diets were 17.4, 20.9, 22.5 and 25.4%, respectively. Results showed that increasing particle size of rice straw and dietary peNDF significantly affected the particle size distributions of digesta in rumen, duodenum and ileum, except feces. However, increasing particle size of rice straw and peNDF did not affected N metabolism in goats, except the increased apparent N digestibility in rumen and large intestine, and the decreased apparent N digestibility in small intestine. Furthermore, increasing particle size of rice straw and peNDF showed little influence on the profile of blood biochemical parameters, microbial AA composition and intestinal AA digestibility in goats.

A model to optimise the requirements of lactating dairy cows for physically effective neutral detergent fibre

This study modelled multiple physiological responses of dairy cows to physical and chemical characteristics of a diet aiming to optimise their requirements for physically effective neutral detergent fibre, expressed inclusive of particles-dry matter > 8 mm (peNDF>8). Extensive research data, comprising a wide range of feeding conditions (n = 64 studies and 257 different dietary treatments), were used to parameterise the model, while statistical modelling was used to account for the inter- and intra-experiment variation as well as to derive the model estimates. Physiological thresholds and ‘safety limits’ of peNDF>8 for maintaining different physiological variables were derived using non-linear statistical modelling. Results showed that peNDF>8 content in the diet is a key factor stimulating rumination activity, maintaining optimal ruminal pH and promoting fibre digestion. Modelling data with regard to the association of fibre digestion and time duration of ruminal pH < 5.8 and dietary peNDF>8 suggests that feeding of less than 13.7% peNDF>8 (the lower ‘safety limit’) is critical to prevent depression of fibre digestion in dairy cows. The study also indicated that the beneficial effects of peNDF>8 on ruminal pH and fibre digestion can be at the expense of the dry matter intake (DMI) level of high-producing cows when the peNDF>8 threshold of 14.9% in the diet is exceeded. In terms of the optimisation of peNDF>8 requirements, the modelling data suggest that feeding of 17–18.5% peNDF>8 can be beneficial in maintaining ruminal pH, while allowing a relatively high DMI (22.3–22.7 kg · d−1) for average high-producing dairy cows.

Effects of Rice Straw Particle Size on Chewing Activity, Feed Intake, Rumen Fermentation and Digestion in Goats

Effects of particle size and physical effective fibre (peNDF) of rice straw in diets on chewing activities, feed intake, flow, site and extent of digestion and rumen fermentation in goats were investigated. A 4x4 Latin square design was employed using 4 mature Liuyang black goats fitted with permanent ruminal, duodenal, and terminal ileal fistulae. During each of the 4 periods, goats were offered 1 of 4 diets that were similar in nutritional content but varied in particle sizes and peNDF through alteration of the theoretical cut length of rice straw (10, 20, 40, and 80 mm, respectively). Dietary peNDF contents were determined using a sieve for particle separation above 8 mm, and were 17.4, 20.9. 22.5 and 25.4%, respectively. Results showed that increasing the particle size and peNDF significantly (p<0.05) increased the time spent on rumination and chewing activities, duodenal starch digestibility and ruminal pH, and decreased ruminal starch digestibility and NH 3 -N concentration. Intake and total tract digestibility of nutrients (i.e. dry matter, organic matter, and starch) and ruminal fermentation were not affected by the dietary particle size and peNDF. Increased particle size and peNDF did not affect ruminal fibre digestibility, but had a great impact on the intestinal and total tract fibre digestibility. The study suggested that rice straw particle size or dietary peNDF was the important influential factor for chewing activity, intestinal fibre and starch digestibility, and ruminal pH, but had minimal impact on feed intake, duodenal and ileal flow, ruminal and total tract digestibility, and ruminal fermentation.

Increasing physically effective fiber content of dairy cow diets through forage proportion versus forage chop length: Chewing and ruminal pH

A study was conducted to evaluate whether the risk of acidosis in dairy cows can be lowered by increasing the physically effective fiber (peNDF) concentration of the diet, either through increased theoretical chop length of alfalfa silage or higher proportion of forage in the diet. The experiment was designed as a replicated 4×4 Latin square using 8 ruminally cannulated lactating dairy cows. Treatments were arranged in a 2×2 factorial design; 2 forage particle lengths (FPL) of alfalfa silage (short and long) were combined with low (35:65) and high (60:40) forage:concentrate (F:C) ratios [dry matter (DM) basis]. Dietary peNDF concentration (DM basis) was determined from the sum of the proportion of dietary DM retained either on the 2 sieves (8 and 19mm) or on the 3 sieves (1.18, 8, and 19mm) of the Penn State Particle Separator multiplied by the neutral detergent fiber concentration of the diet. The dietary peNDF concentrations were altered by changing the F:C or the FPL, and ranged from 10.7 to 17.5% using 2 sieves, or from 23.1 to 28.2% using 3 sieves. Intake of peNDF was increased by increasing FPL but not by increasing F:C ratio because of the reduction of DM intake at the higher F:C ratio. Chewing activity, including number of chews and chewing time, increased with increasing F:C ratio or FPL. Mean ruminal pH was elevated by 0.4 and 0.2 units with increasing F:C ratio and FPL, respectively. Lowering the F:C ratio decreased the duration that ruminal pH was below 5.8 (1.2 vs. 8 h/d). Increased F:C ratio or FPL reduced ruminal volatile fatty acids concentration from 137 to 122 or from 133 to 126mM, respectively, whereas acetate:propionate ratio was increased from 2.55 to 3.46 with increasing F:C ratio. Dietary peNDF concentration measured using 2 sieves was correlated to chewing time (r=0.57) and mean ruminal pH (r=0.75), whereas dietary peNDF concentration measured using 3 sieves was correlated to mean ruminal pH (r=0.83) and negatively correlated to the time that pH was below 5.8 (r=−0.78). This study shows that the risk of ruminal acidosis is high for cows fed a low F:C diet. Increasing the proportion of forage in the diet helps prevent ruminal acidosis through increased chewing time, a change in meal patterns, and decreased ruminal acid production. Increasing FPL elevates ruminal pH, but in low forage diets, increased FPL does not alleviate subacute acidosis because the fermentability of the diet is high and changes in chewing activity are marginal.

Evaluation of the Response of Ruminal Fermentation and Activities of Nonstarch Polysaccharide-Degrading Enzymes to Particle Length of Corn Silage in Dairy Cows

The main objective of this study was to evaluate effects of particle length (PL) of corn silage (CS) on distribution of dietary particle fractions, contents of physically effective neutral detergent fiber (peNDF), cows’ intake patterns and sorting activity, fermentation pro-file, and activities of nonstarch polysaccharide-degrading enzymes as well as degradation in the rumen and total tract in lactating dairy cows. Four ruminally cannulated Holstein cows, weighing 624±50kg and 60±8 d in milk, were fed ad libitum 3 total mixed rations [about 16% crude protein, 34% neutral detergent fiber (NDF), and 7 MJ of net energy of lactation/kg of dry matter (DM)] containing on DM basis 50% concentrate, 10% grass hay, and 40% CS with 3 different theoretical PL at harvesting (14, 8.1, and 5.5mm for long, medium, and short, respectively). Results showed that the amount of DM retained on sieves with 19- and 8-mm screens of Penn State Particle Separator decreased linearly with decreasing PL of CS. The latter was reflected in a significant decrease in the content of dietary peNDF including both the DM (peNDF>8) and the NDF (peNDF>8-NDF) retained on 19- and 8-mm screens. In contrast, the fraction of particles retained between the 1.18- and 8-mm screens was increased, such that no differences among the diets were observed regarding the content of peNDF that includes DM of particles >1.18mm (peNDF>1.18). The intake of particles retained between the 1.18- and 8-mm screens increased linearly, whereas the intake of peNDF>1.18 increased quadratically with decreasing PL of CS. Sorting consumption was reduced by feeding the short CS, which was reflected in a reduced proportion of propionate and increased acetate-to-propionate ratio and butyrate pro-portion in the rumen. In contrast, no effects of PL of CS were observed on the concentration of total volatile fatty acids and pH in the rumen. In general, decreasing the PL of CS significantly increased the activities of nonstarch polysaccharide-degrading enzymes. However, greater ruminal and total tract degradation of fiber and nonfiber carbohydrates were observed only by medium CS. Results of the present study suggest that in addition to fractions of long particles (i.e., >8mm), the particle fraction retained between 1.18- and 8-mm should also be considered to better predict rumen conditions and digestion. In conclusion, a moderate reduction of PL of CS has beneficial effects on nutrient digestion, and may maximize feed efficiency and energy supply in high-yielding dairy cows.

Altering Physically Effective Fiber Intake Through Forage Proportion and Particle Length: Digestion and Milk Production

Intake of physically effective neutral detergent fiber (peNDF) of dairy cows was altered by adjusting the proportion of forage in the diet and forage particle length, and effects on nutrient intake, site and extent of digestion, microbial N synthesis, and milk production were measured. The experiment was designed as a triplicated 4×4 Latin square using 12 lactating dairy cows, with 4 that were ruminally and duodenally cannulated, 4 that were ruminally cannulated, and 4 that were intact. Thus, the site and extent of digestion, and microbial N synthesis were measured in a single 4×4 Latin square. Treatments were arranged in a 2×2 factorial design; 2 forage particle lengths (FPL) of alfalfa silage (short and long) were combined with low (35:65) and high (60:40) forage:concentrate (F:C) ratios (dry matter basis). Dietary peNDF content was determined from the sum of the proportion (dry matter basis) of dietary dry matter retained either on the 2 screens (8- and 19-mm) or on the 3 screens (1.18-, 8-, and 19-mm) of the Penn State Particle Separator multiplied by the neutral detergent fiber content of the diet. An increased F:C ratio reduced intakes of dry matter and starch by 9 and 46%, respectively, but increased intake of fiber from forage sources by 53%. Digestibility of dry matter in the total tract was not affected, whereas total digestion of fiber and N was improved by increasing the F:C ratio. Improved total fiber digestion resulted from higher ruminal digestion, which was partially due to a shift in starch digestion from the rumen to the intestine with the increased F:C ratio. Actual milk yield was decreased but production of 4% fat-corrected milk was similar between the low and high F:C diets because of increased milk fat content. Increased FPL increased intake of peNDF, especially when the high F:C diet was fed. However, nutrient intakes, N metabolism in the digestive tract, and milk production were not affected. Digestibility of neutral detergent fiber in the total tract was increased because of improved fiber digestion in the rumen with increased FPL. These results indicate that feeding dairy cows a low F:C diet is beneficial in terms of increasing feed intake, microbial N synthesis, and milk production. However, low F:C diets do not maximize feed digestion and production efficiency because of the effects of subacute ruminal acidosis. Increased FPL improves fiber utilization with minimal effects on the digestion of other nutrients and milk production. Increasing dietary peNDF, through an increased proportion of forage or increased FPL, improves fiber digestion because of improved rumen function.

Altering Physically Effective Fiber Intake Through Forage Proportion and Particle Length: Chewing and Ruminal pH

Alfalfa silages varying in theoretical chop length and diets high and low in forage proportion were used to evaluate whether increasing the physically effective (pe) neutral detergent fiber (NDF) content of dairy cow diets reduces the risk of acidosis. The experiment was designed as a replicated 4×4 Latin square using 8 ruminally cannulated lactating dairy cows. Treatments were arranged in a 2×2 factorial design; 2 forage particle lengths (FPL) of alfalfa silage (short and long) were combined with low (35:65) and high (60:40) forage:concentrate (F:C) ratios [dry matter (DM) basis]. Dietary peNDF content (DM basis) was determined from the sum of the proportion of dietary DM retained on either the 2 sieves (8 and 19mm) or the 3 sieves (1.18, 8, and 19mm) of the Penn State Particle Separator multiplied by the NDF content of the diet. The dietary peNDF contents ranged from 9.6 to 19.8% using 2 sieves, or from 28.6 to 34.0% using 3 sieves. Intake of peNDF was increased by increasing both the F:C ratio and the FPL of the diets. However, F:C ratio and FPL affected chewing activity differently; increasing F:C ratio increased chewing time but increasing FPL only increased chewing when a high-forage diet was fed. Mean ruminal pH was increased by 0.5 and 0.2 units with increasing F:C ratio and FPL, respectively. Cows fed the low F:C diet had>10 or 7h daily in which ruminal pH was below 5.8 or 5.5, respectively, compared with 1.2 and 0.1h for cows fed the high F:C ratio diet. Increased F:C ratio reduced ruminal VFA concentration from 135 to 121mM but increased the acetate:propionate ratio from 1.82 to 3.13. Dietary peNDF content when measured using 2 sieves was positively correlated to chewing time (r = 0.61) and mean ruminal pH (r = 0.73), and negatively correlated to the time that pH was below 5.8 or 5.5 (r = −0.46). This study shows that the risk of ruminal acidosis is high for cows fed a low F:C diet, particularly when finely chopped silage is used. Intake of dietary peNDF is a good indicator of ruminal pH status of dairy cows. Increasing the proportion of forage in the diet helps prevent ruminal acidosis through increased chewing time, a change in meal patterns, and decreased ruminal acid production. Increasing FPL elevates ruminal pH, but in low-forage diets increased FPL does not completely alleviate subacute acidosis because the fermentability of the diet is high and changes in chewing activity are marginal.

Physically Effective Fiber: Method of Determination and Effects on Chewing, Ruminal Acidosis, and Digestion by Dairy Cows

A study was conducted to investigate the effects of physically effective neutral detergent fiber (peNDF) content of dairy cow diets containing corn silage as the sole forage on intake, chewing, ruminal pH, microbial protein synthesis, digestibility, and milk production. A second objective was to compare current methods of measuring peNDF to determine the most suitable approach for use in ration formulation. The experiment was designed as a replicated 3×3 Latin square using 6 lactating dairy cows with ruminal cannulas. Diets varied in peNDF content (high, medium, and low) by altering the particle length of corn silage. The physical effectiveness factors (pef) and peNDF contents of the corn silage and diets were determined based on the original (19- and 8-mm sieves) and new Penn State Particle Separator (PSPS; 19-, 8-, and 1.18-mm sieves). A dry-sieving technique that measures the proportion of particles retained on a 1.18-mm sieve was also used. The new PSPS and the 1.18-mm sieve produced similar estimates of pef and peNDF of diets but gave higher values than the original PSPS. There was a much smaller range in pef of corn silage when 3 sieves, rather than 2, were used with the PSPS (range of 0.93 to 0.96 vs. 0.41 to 0.72, respectively). Consequently, increased forage particle length in the diets increased dietary peNDF content and its intake when using the original PSPS; however, the new PSPS and the 1.18-mm sieve failed to detect changes in dietary peNDF and peNDF intake. The peNDF values estimated based on fractional NDF rather than the total NDF content were higher, but the ranking of diets was not changed. Increased intake of peNDF linearly increased digestibility of CP and tended to linearly increase digestibility of fiber in the total tract. As a result, milk yield tended to linearly increase with no effect on milk composition. Ruminal microbial protein synthesis and microbial efficiency were higher with the medium peNDF than with the high or low peNDF diets. Total chewing time and ruminating time were linearly increased with increasing dietary peNDF, whereas influence of dietary peNDF on ruminal pH and fermentation was minimal. This study showed that increasing peNDF content of diets improved fiber digestion. Of the systems used to measure peNDF, the original PSPS provided a good description of dietary particle length and its effects on chewing time and rumen pH, whereas the new PSPS provided a more consistent chewing index, the ratio of total chewing activity to peNDF, across diets varying in chop length of corn silage.

Increasing the Physically Effective Fiber Content of Dairy Cow Diets May Lower Efficiency of Feed Use

Barley silages varying in theoretical chop length were used to evaluate the effects of physically effective (pe) neutral detergent fiber (NDF) content of dairy cow diets on nutrient intakes, site and extent of digestion, microbial protein synthesis, and milk production. The experiment was designed as a replicated 3×3 Latin square using 6 lactating dairy cows with ruminal and duodenal cannulas. During each of 3 periods, cows were offered 1 of 3 diets (low, medium, and high peNDF) obtained using barley silage that varied in particle length: fine (theoretical chop length of 4.8mm), medium (equal proportions of long and fine silages), and long (theoretical chop length of 9.5mm). The peNDF contents were determined by multiplying the proportion (dry matter basis) of feed retained on the 2 screens (8 and 19mm) of the Penn State Particle Separator by the NDF content of the diet, and were 10.5, 11.8, and 13.8% for the low, medium, and high diets, respectively. Increased forage particle length linearly increased intake of peNDF but intakes of dry matter, organic matter, starch, and N were highest for cows fed the medium peNDF diet. Digestibilities of organic matter, NDF, and acid detergent fiber in the total tract were linearly decreased with increasing dietary peNDF, although total digestibility of starch and N was not affected by the treatments. Nevertheless, decreased digestibility due to increased dietary peNDF did not reduce milk production or milk composition because the cows were in mid to late lactation. Ruminal microbial protein synthesis and microbial efficiency were numerically higher with the low peNDF than with the medium or high peNDF diets. These results indicate that increasing the peNDF content of a diet containing barley silage decreases fiber digestibility in the total tract and lowers microbial efficiency. Therefore, the benefits of increasing dietary particle size, expressed as peNDF, on reducing the risk of ruminal acidosis should be weighed against potentially negative effects on efficiency of feed use.

Effects of Physically Effective Fiber on Digestive Processes and Milk Fat Content in Early Lactating Dairy Cows Fed Total Mixed Rations

Data from recent research studies were analyzed quantitatively, and the random effect of experiment was assessed to define the physiological responses of dairy cows in early lactation to intake of physically effective neutral detergent fiber (peNDF). All studies were conducted with lactating Holstein cows (84.8±3.54 days in milk) in Latin square designs, and feeds were offered ad libitum as total mixed rations (TMR). The peNDF was estimated by 2 measurement techniques, the NDF content of TMR multiplied by amount of dry matter (DM) retained on a 1.18-mm screen (peNDF> 1.18) and NDF content of TMR multiplied by the proportion of DM retained by 19- and 8-mm Penn State Particle Separator screens (peNDF> 8). Other factors, including concentrations of NDF, forage NDF, non-fiber carbohydrates, the amount of digestible organic matter of forages (FDOM), and the intake of ruminally degradable starch (RDSI) from grain in the diet were also investigated. The studied animal response variables included feed intake, ruminal fermentation, chewing activity, fiber digestibility, and milk production and composition. The ruminal pH (day mean) in this study ranged from 5.30 to 6.59. Using peNDF> 1.18 approach, the requirements for physically effective fiber in high-yielding dairy cows fed TMR in an ad libitum intake were estimated to be about 19% of ration DM or 4.1kg/d or 0.6kg/100kg of body weight to maintain a ruminal pH of about 6.0. When peNDF was measured as peNDF> 8, ruminal pH responded in a quadratic fashion but the confidence of estimation was lower (R2=0.27) compared with the peNDF> 1.18 approach (R2=0.67). Results of these data analyses showed that peNDF> 1.18 provided a satisfactory estimation of the mean ruminal pH (R2=0.67) and NDF digestibility (R2=0.56). Furthermore, peNDF> 1.18 was poorly, although positively, correlated to daily chewing (R2=0.17), and rumination (R2=0.24) activity. On the other hand, results from these analyses showed that milk parameters are less sensitive to the effects of dietary peNDF than other variables, such as ruminal pH, chewing activity, and fiber digestibility. Dietary FDOM correlated positively (moderately) to ruminal pH (R2=0.24), daily chewing (R2=0.23), and rumination (R2=0.29) activity, whereas the daily RDSI from grain correlated negatively to ruminal pH (R2=0.55) and positively to total volatile fatty acids (R2=0.27). Inclusion of FDOM and RDSI from grain along with peNDF> 1.18 in the models that predict rumen pH further improved the accuracy of prediction. This approach appeared to further complement the concept of peNDF that does not account for differences in ruminal fermentability of feeds.

Effects of Physically Effective Fiber on Chewing Activity and Ruminal pH of Dairy Cows Fed Diets Based on Barley Silage

The objective of this study was to investigate the effects of physically effective neutral detergent fiber (peNDF) content of dairy cow diets containing barley silage as the sole forage source on feed intake, chewing activity, and ruminal pH. The experiment was designed as a replicated 3×3 Latin square using 6 lactating dairy cows with ruminal cannulas. Cows were offered 1 of 3 diets (high, medium, and low peNDF) obtained using barley silage that varied in particle length: long (theoretical cut length of 9.5mm), medium (equal proportions of long and fine silages), and fine (theoretical cut length of 4.8mm). The peNDF contents were determined using the Penn State Particle Separator and were 13.8, 11.8, and 10.5%, for the high, medium, and low diets, respectively. The physical effectiveness factors (defined as proportion retained on 19- and 8-mm screens) for the long and fine silages were 0.84 and 0.68, respectively. Increased forage particle size increased intake of peNDF but did not affect intake of DM and NDF. Ruminating and total chewing time were linearly increased with increasing dietary peNDF. Mean ruminal pH, area between the curve and a horizontal line drawn at pH 5.8 or 5.5, and time that pH was below 5.8 or 5.5 were not affected by peNDF content. Intake of peNDF was not correlated to any chewing activity but proportion of long particles on the 19-mm sieve tended to be correlated to ruminating chews (r = 0.36) and ruminating time (r = 0.36). These results indicate that increasing the peNDF content of diets increases chewing time. However, increased chewing time does not always improve ruminal pH status. Increasing chewing time and thus increasing salivary secretion may not fully overcome the effects of feed digestion and the production of fermentation acids that lower rumen pH. The results suggest that dietary peNDF and fermentable OM intake are critical in regulating rumen pH. Dietary particle size, expressed as peNDF, was a reliable indication of chewing activity.