Dietary Intake Recommendations Research Articles

Micronutrient Patterns and Low Intake of Vitamin A, Vitamin D, Folate, Magnesium, and Potassium Among Prediabetes and Type 2 Diabetes Patients.

Background Assessing micronutrient intake is important in identifying deficiencies that may contribute to insulin resistance, poor glycemic control, and increased risk of diabetes-related complications. The study's objectives were to evaluate micronutrient intake in prediabetes (PD) and type 2 diabetes (T2DM) patients compared to recommended dietary intakes (RDI) and to determine the associations between the micronutrient patterns and both anthropometric measurements and biomarkers of diabetes. Methods This cross-sectional study was conducted on 349 patients with T2DM and 252 patients with PD. Micronutrient intake was evaluated using a validated food frequency questionnaire. Micronutrient patterns were extracted from factor analysis using principal component analysis with varimax rotation. Participants in the highest tertile were considered to have the highest adherence to the corresponding micronutrient pattern. Results T2DM patients had a significantly lower intake of vitamin E (9.4 ± 0.2 vs. 10.1 ± 0.3 mg; p = 0.048), vitamin D (44.3 ± 1.1 vs. 48.9 ± 1.7 IU; p = 0.020), and thiamin (1.3 ± 0.1 vs. 1.4 ± 0.1 mg; p = 0.013) compared to PD patients. All patients had a significantly lower intake of vitamin A, vitamin D, folate, magnesium, and potassium and a significantly higher intake of vitamin B12 and copper compared to RDI. Three distinct micronutrient patterns were identified within each group. In the PD group, the Fe-Mn-Se pattern correlated significantly with waist circumference (WC) and fasting plasma glucose (FPG). The Vit.C-K-Folate pattern showed significant associations with body fat (BF). The Vit.B2-P-Vit.B12 pattern was significantly linked to WC, body mass index (BMI), BF, FPG, and serum insulin (SI). For the T2DM patients, the K-Folate-Mg pattern displayed an inverse and significant association with weight and WC. The Iron-Se-Vit.B3 pattern showed a significant association with low-density lipoprotein (LDL) cholesterol, triglycerides, and total cholesterol. The Vit.B2-P-Ca pattern was significantly associated with fasting plasma glucose (FPG). Conclusion This study demonstrated that T2DM patients had significantly lower vitamin E, vitamin D, and thiaminintake than PD patients. Both T2DM and PD patients had a significantly lower intake of vitamin A, vitamin D, folate, magnesium, and potassium compared to the RDI. Among the identified micronutrient patterns, only the K-Folate-Mg pattern exhibited a significant association withreduced body weight and WC.

Global guidelines recommendations for dietary sodium and potassium intake.

Non-communicable diseases (NCDs) cause a significant global health challenge, with unhealthy diets identified as a major risk factor. Sodium and potassium, which are essential minerals for human health, play important roles in various bodily functions, and an imbalance in their intake can have significant health implications, particularly concerning hypertension and cardiovascular diseases. This review compiles dietary sodium and potassium intake recommendations from prominent global health organizations and compares global guidelines to Japan's Dietary Reference Intake (DRI) guidelines. Sodium and potassium intake guidelines from organizations such as the World Health Organization (WHO), American College of Cardiology (ACC) and American Heart Association (AHA), Dietary Guidelines for Americans (DGA), European Food Safety Authority (EFSA), and DRI for Japanese exhibit variations. Compared to other Asian countries, Japan's historically higher sodium goal aligns with Southeast Asia where traditional preserved foods contribute to high sodium intake. Contrarily, Japan's lower potassium goal contrasts with other countries in Asia promoting a diet rich in fruits and vegetables. The ongoing effort by Japan to align with global recommendations reflects a gradation approach considering social habits. While harmonizing international efforts is essential, appreciating regional diversities is paramount through tailoring guidelines to cultural and dietary habit practices. Implementing context-specific guidelines informed by scientific research can contribute to global efforts in promoting healthy diets and reducing the burden of NCDs. Global guidelines that recommended the daily dietary intake goal for sodium and potassium exhibit variations. These disparities are influenced by diverse factors, including cultural dietary habits, socioeconomic status, health priorities, and available scientific research. Each population should follow the recommendations of their region.

More dietary advice may be needed to support healthy body composition for children with cerebral palsy in Aotearoa New Zealand

The available literature on the nutritional status of children with cerebral palsy (CP) worldwide has identified high rates of malnutrition, specifically undernutrition(1). However, there is a current lack of clear CP-specific dietary guidelines for children with CP across all functional levels. Standard reference tools such as estimated energy requirement (EER) and recommended dietary intake (RDI) may overestimate requirements in children with CP, especially those with reduced mobility and activity levels. Furthermore, for children with severe CP, body composition data indicates higher risks of obesity and obesity-related conditions(2). There can be a wide range of functional abilities, classifiable with tools such as the Gross Motor Function Classification System (GMFCS) and the Eating and Drinking Ability Classification System (EDACS). The majority of nutrition-related CP literature focuses on children requiring assistance for feeding (EDACS IV-V) with little information available for children with higher levels of functional independence. The aim of this study was to determine whether children with CP had received any prior dietary guidance for healthy body composition and to summarise the type of advice received. Children aged 5-12 years and their whānau were invited to participate in a study where a purpose-developed questionnaire captured their history of receiving tailored dietary recommendations for CP. Body composition was assessed via whole-body dual-energy X-ray absorptiometry scan. Nine participants (6 males, median age: 10y, n = 2 Māori), across GMFCS levels I-IV and EDACS levels I-III took part in the study. Out of 9 children, 5 (55%) indicated that their child had never received dietary advice, 3 of whom were classified as obese or overweight based on growth chart percentiles using their measures of body fat percentage, 1 was classified as underweight and 1 was within the healthy ranges. Of the 4 who had previously received advice, its nature was reported as in support of weight gain (n = 3), and weight loss (n = 1). All 4 received dietary advice from a dietitian and 1 reported some additional advice from a pediatrician and/or orthopedic doctor. Two of the children who had received dietary advice fell within a healthy body fat percentage based on the growth chart percentiles at the time of the study, while the other two were classified as overweight or obese. The results indicate the importance of developing clear dietary guidance for children with CP which may differ from that for typically developing children, particularly depending upon CP subtype diagnosis and functional level, in order to support healthy body composition.

In Vitro bioaccessibility evaluation of minerals in Camu-Camu and Spirulina dietary supplements

This study aimed to determine the total concentration of minerals (Mg, Ca, Zn, Fe, Mn, and Cu) in Spirulina and camu-camu dietary supplements, to evaluate the bioaccessibility of minerals using in vitro digestion method, and to assess the correlation with protein, ash, lipids, dietary fibers and with the bioaccessible fraction (BF) of minerals. The vitamin C content of camu-camu was also determined, and its correlation with the mineral bioaccessible fraction was evaluated. Spirulina presented higher bioaccessible fractions in the gastrointestinal phase (%) for Mg (82−91), Zn (82−90), and Mn (64−81), followed by Cu (59−100), Ca (50−76), and Fe (37−45). Camu-camu supplements presented higher bioaccessible fractions (%) for Cu (100), Fe (32−104) and Ca (64−89), followed by Mn (7−93), Mg (4−99) and Zn (31−51), in the gastrointestinal phase. Mineral bioaccessibility showed negative correlations with the matrix, mainly with dietary fiber. It was evident that vitamin C was found to have favored the bioaccessibility of Fe and Ca in camu-camu. For 1 g intake of Spirulina, BF Fe contributes 6.6% of the Recommended Dietary Intake (RDI) of Fe and 3.6% of the RDI of Mg for camu-camu. Based on this study, an insight into the nutritional value of Spirulina and camu-camu in terms of minerals and the correlation with proximate composition was demonstrated.

Harnessing the power of resistant starch: a narrative review of its health impact and processing challenges.

Starch is a primary energy storage for plants, making it an essential component of many plant-based foods consumed today. Resistant starch (RS) refers to those starch fractions that escape digestion in the small intestine and reach the colon where they are fermented by the microflora. RS has been repeatedly reported as having benefits on health, but ensuring that its content remains in food processing may be challenging. The present work focuses on the impact RS on health and explores the different processes that may influence its presence in foods, thus potentially interfering with these effects. Clinical evidence published from 2010 to 2023 and studying the effect of RS on health parameters in adult populations, were identified, using PUBMED/Medline and Cochrane databases. The search focused as well on observational studies related to the effect of food processes on RS content. While processes such as milling, fermentation, cooking and heating seem to have a deleterious influence on RS content, other processes, such as cooling, cooking time, storage time, or water content, may positively impact its presence. Regarding the influence on health parameters, there is a body of evidence suggesting an overall significant beneficial effect of RS, especially type 1 and 2, on several health parameters such as glycemic response, insulin resistance index, bowel function or inflammatory markers. Effects are more substantiated in individuals suffering from metabolic diseases. The effects of RS may however be exerted differently depending on the type. A better understanding of the influence of food processes on RS can guide the development of dietary intake recommendations and contribute to the development of food products rich in RS.

Women in Canada are consuming above the upper intake level of folic acid but few are meeting dietary choline recommendations in the second trimester of pregnancy: data from the CHILD cohort study.

There is concern that during a low-risk pregnancy, women are consuming more than recommended (400 µg/day) supplemental folic acid and may not meet recommendations for other nutrients. The objective of this study was to determine folic acid supplement use and dietary folate intakes in the second trimester (week 18) of pregnancy in women (n=2996) in the Canadian CHILD cohort study. Vitamin B12 and choline intakes were also assessed because they are metabolically related to folate. The majority of participants (71.6%) were consuming a daily prenatal supplement. Twenty-eight percent of women (n=847) reported consuming a folic acid supplement and of these women, 45.3% had daily supplemental folic acid intakes above the upper intake level (UL; 1000 µg/day). Daily dietary folate intakes were (mean (SD)) 575 (235) DFE µg/day. In contrast, only 24.8% of women met the dietary choline adequate intake (AI) recommendation (AI ≥ 450mg/day) with a mean (SD) intake of 375 (151) mg/day. Further understanding of the impact of supplemental folic acid intake above the UL and low choline intake during pregnancy requires further investigation.

Three approaches to assessing dietary quality in Mexican adolescents from 2006 to 2018 with data from national health and nutrition surveys.

To assess trends in the dietary quality of Mexican adolescents from 2006 to 2018, both overall and by sociodemographic indicators, using adaptations of the EAT-Lancet Planetary Health (PH) recommendations, optimal intake estimated by the Global Burden of Disease (GBD) and 2015 Mexican Dietary Guidelines (MDG) in nationally representative samples. Using dietary data from a semi-quantitative FFQ, dietary quality indexes were constructed as adaptations of three dietary intake recommendations. Trends in adherence to recommendations were evaluated with multivariate quantile regression models with survey year as the main independent variable and adjusted for age, sex, energy intake, dwelling area, geographical region, household assets condition, and student/non-student status. P values and CI were Bonferroni-corrected. Mexico. Non-pregnant or lactating adolescents aged 12-19 years (n 16 520). Adherence to the PH index was about 40 %, GBD was nearly 35 % and MDG was about 37 %. The lowest adherences were for added sugars, sugar-sweetened beverages, nuts and seeds, red meats, processed meats, and legumes (<28 %). No 2006-2018 trends in total adherence were found in any index. Nevertheless, negative adherence trends were identified for poultry (β = -2·4), and saturated fats (β = -0·93), and positive for unsaturated oils (β = 1·23), in the PH. In MDG, relevant trends were found for plain water (β = 1·63) and foods rich in fats (β = -1·24). Mexican adolescents have demonstrated poor dietary quality by these three approaches. Therefore, this population has a high-risk profile for diet-associated chronic diseases. Further research and appropriate public policies are needed.

Promising Practices to Promote Physical Activity and Healthy Eating Among Head Start Caregivers and Their Children

Many children in Head Start programs do not meet physical activity (PA) and dietary intake recommendations and have increased risk for obesity. The purpose of this pilot study was to formatively assess a multi-level intervention, exploring strategies to encourage PA and healthy eating among north Texas Head Start families. In year 1, 5 Head Start sites with 217 children began the Head Start to Healthy Lifestyles (HSHL) project. Direct and indirect education strategies began in the last 2 months of the school year. Surveys were used to evaluate child and caregiver PA, healthy eating behaviors, and interagency collaboration. Strategies and barriers to adopting healthy lifestyles were assessed via grant partner discussions. Of 217 children enrolled, 109 caregivers consented to participate in research, of which 35 completed baseline surveys and 10 also submitted post surveys. The number of fruits eaten by Head Start caregivers improved ( P &lt; .05). Perceived level of collaboration among grant partners was high. Strategies appreciated by Head Start site partners included PA support in the classrooms, take-home gardens, and reading food group books. Additional needed support included tasting and on-site cooking classes. Barriers to participation included few face-to-face meetings with parents, staffing shortages post-COVID, and difficulty engaging families.

Proximate and Mineral Composition of Noodles Incorporated with Moringa and Sardine Powders

This study aims to investigate the effect of incorporating Moringa oleifera leaf powder and sardine powder into wheat-based noodle formulations on their nutritional composition. Different formulations were prepared with varying ratios, expressed in percentage (%): WM1 (99.6 wheat: 0.4 moringa ), WM2 (99.2 wheat: 0.8 moringa) andWM3 (99 wheat :1 moringa ); WS1 (95 wheat: 5 sardine ), WS2 (90 wheat: 10 sardine ), WS3 (85 wheat: 15 sardine) and the control sample (WC) containing 100% wheat was also included for comparison. The nutritional parameters assessed included crude protein, crude fiber, crude fat, ash, carbohydrate and energy content. Mineral compositions were also determined, including calcium (Ca), magnesium (Mg), iron (Fe) and zinc (Zn). The results demonstrated significant variations in the nutritional composition of the different formulations compared to the control sample. Results of proximate composition based on dry matter showed that the crude protein values ranged from 11.76-21.40g/100g, crude fibre values ranged from 0.00-0.02g/100g, crude fat values ranged from 1.89-2.94g/100g, ash content values ranged from 1.38-2.26g/100g, carbohydrate content values ranged from 62.31-74.29g/100g and energy values ranged from 358.50-364.53 kcal/100g. Furthermore, the minerals results showed iron values ranged from 32.55-65.50mg/100g, calcium values ranged from 7.39-66.61g/100g, magnesium values ranged from 28.86-87.35mg/100g and zinc values ranged from 2.21-39.25mg/100g. These results indicate that the fortification of noodles with moringa and sardine powders can be a viable approach for enhancing the nutritional value of the final product. Keywords: Proximate composition, Micronutrients, Moringa leaf, Sardine, Recommended Dietary Intake.

Assessment of Anthropogenic Impact on Ecosystem Service Safety of Agboyi River in Lagos, Southwestern, Nigeria

The Agboyi River in Lagos, Nigeria provides important ecosystem services; however, anthropogenic activities are polluting the river, necessitating periodic monitoring. This study assessed the heavy metal content (lead, cadmium, chromium, copper, and arsenic) in water samples from the river. Additionally, we evaluated various physicochemical parameters, including pH, total dissolved solids (TDS), conductivity, hardness, magnesium, calcium, chloride, sulfate, and nitrate. The values of each heavy metal were used to calculate human average daily ingestion (ADI), average daily dermal exposure (ADDE), hazard quotient (HQ), and carcinogenic risk (CR). The physicochemical analysis revealed non-permissible levels of TDS, electrical conductivity, alkalinity, hardness, magnesium, calcium, sulfate, nitrate, chloride, and phosphate. The heavy metal analysis showed intolerable levels of lead, arsenic, cadmium, chromium, and copper. The ADIs for the heavy metals were within the recommended dietary intake (RDI), but their ADDEs exceeded the RDI, except for chromium. The HQ and CR for all heavy metals exceeded recommended limits. Seasonal variations were observed in the physicochemical parameters, with TDS, turbidity, acidity, nitrate, and phosphate being higher in the wet season, while other parameters were higher in the dry season. The water poses health hazards to users, indicating the need for river remediation.

A mixed-methods approach to understanding barriers and facilitators to healthy eating and exercise from five European countries: highlighting the roles of enjoyment, emotion and social engagement

Healthy adults are consistently falling below national and international recommendations for physical activity and dietary intake across Europe. This study took a co-creative approach with adult samples from five European countries to qualitatively and quantitatively establish motivators, barriers and sustaining factors for positive health behaviour change. Stage 1 delivered a newly-designed online programme, creating a community who identified challenges, motivators and solutions to sustaining positive healthy eating and physical activity behaviours. Stage 2 administered an online survey (developed from Stage 1 findings) to a larger sample to quantify the relative importance of these motivators and barriers. Results from both stages indicated enjoyment, positive emotions, and reward as key motivators for both behaviours across all five countries. Barriers included habit-breaking difficulties, temptation and negative affective states. Those with a high BMI placed more importance on social pressure than those with healthy BMI. Participants’ reports of motivators and barriers reflected relevant approaches from consumer science, behavioural economics, and psychology. Interventions supporting adults who are not chronically ill but would benefit from improved diet and/or physical activity should not focus exclusively on health as a motivating factor. Emphasis on enjoyable behaviours, social engagement and reward will likely improve engagement and sustained behaviour change.

Dietary protein recommendations to support healthy muscle ageing in the 21st Century and beyond: considerations and future directions.

This review explores the evolution of dietary protein intake requirements and recommendations, with a focus on skeletal muscle remodelling to support healthy ageing based on presentations at the 2023 Nutrition Society summer conference. In this review, we describe the role of dietary protein for metabolic health and ageing muscle, explain the origins of protein and amino acid (AA) requirements and discuss current recommendations for dietary protein intake, which currently sits at about 0⋅8 g/kg/d. We also critique existing (e.g. nitrogen balance) and contemporary (e.g. indicator AA oxidation) methods to determine protein/AA intake requirements and suggest that existing methods may underestimate requirements, with more contemporary assessments indicating protein recommendations may need to be increased to &gt;1⋅0 g/kg/d. One example of evolution in dietary protein guidance is the transition from protein requirements to recommendations. Hence, we discuss the refinement of protein/AA requirements for skeletal muscle maintenance with advanced age beyond simply the dose (e.g. source, type, quality, timing, pattern, nutrient co-ingestion) and explore the efficacy and sustainability of alternative protein sources beyond animal-based proteins to facilitate skeletal muscle remodelling in older age. We conclude that, whilst a growing body of research has demonstrated that animal-free protein sources can effectively stimulate and support muscle remodelling in a manner that is comparable to animal-based proteins, food systems need to sustainably provide a diversity of both plant and animal source foods, not least for their protein content but other vital nutrients. Finally, we propose some priority research directions for the field of protein nutrition and healthy ageing.

Nutrition facts labels: who is actually reading them and does it help in meeting intake recommendations for nutrients of public health concern?

BackgroundFiber, potassium and calcium are nutrients of public health concern and their intakes in the United States are alarmingly low. The usage of nutrition facts labels has been reported to increase the odds for dietary reference intake of fiber in some studies. The overall evidence, however, is mixed, as some studies suggested that nutrition facts panels have little to no effect on average measures of diet quality. Here, we investigated whether the usage of nutrition facts labels was associated with meeting U.S. intake recommendations for three nutrients of public health concern: fiber, potassium and calcium.MethodsWe used cross-sectional multistage, stratified, clustered and probability sampling design data from the U.S. National Health and Nutrition Examination Surveys 2017–2020 cycle. The sample included 5,416 individuals aged 20 years or older, which may be extrapolated to represent 146,841,866 US Americans. Nutrient intakes were compared among individuals reading nutrition facts panels “frequently”, “sometimes” or “rarely” using applied survey data analyses techniques (including multivariate logistic regression and marginsplots).ResultsWe observed substantial sociodemographic differences between the three groups. Frequent readers were significantly more likely to be female and had higher educational levels. On average, they were also significantly older as compared to rare readers. Fiber intake in g/d was highest in frequent readers (17.09) and lowest in rare readers (14.64). The proportion of participants that met dietary fiber intake recommendations was almost four times higher in the frequent readers group (12.69%) as compared to the rare readers group (3.69%). In a bivariate logistic regression model, frequent label reading significantly increased the odds for meeting the fiber recommendations in Dietary Guidelines for Americans (OR: 2.15, p < 0.001). Rarely reading labels decreased the odds (OR: 0.57, p = 0.003). These odds remained essentially unchanged after adjusting for sociodemographic covariates, diabetes status and body mass index (OR: 1.84, p = 0.004; and OR: 0.62, p = 0.022).ConclusionsNutrition facts panel reading associates with fiber intake. Our findings have potential implications for public health nutrition strategies that may center around educational work.

Nutritional Composition, Capsaicin Content and Enzyme Inhibitory Activities from “Bang Chang” Thai Cultivar Chili Pepper (Capsicum annuum Var. acuminatum) after Drying Process

Chili pepper cultivars come in a broad variety of tastes, colors, sizes, and spiciness levels due to various growth conditions. The significance of different chili peppers and other spices in Thai cuisine may be seen in dishes. This study evaluated the nutritional composition and capsaicin content from “Bang Chang” Thai cultivar chili pepper after drying, and the inhibition of lipase, tyrosinase and elastase enzymes from its oil and ethanol extracts. The nutritional composition of sun-dried chili pepper was high dietary fiber and beta-carotene, which were significantly high amount portion of Thai recommended dietary intake (RDI). According to calculations and definitions, “Bang Chang chili pepper” is a non-pungent Capsicum (0-700 SHU) since it contains a low amount of capsaicin. Total phenolic content was more abundant in ethanol extract (2.50 ± 0.13 mg GAE/g) than oil extract (1.05 ± 0.05 mg GAE/g). Compared to the positive control orlistat (IC50 = 3.26 ± 0.28 mg/mL), an anti-lipase drug, ethanol extract was 2.0 times more lipase inhibitory. In addition, ethanol extract was mildly anti-tyrosinase, while oil extract was non-activity. The anti-lipase activity of ethanol extract was due to phenolic content rather than capsaicin contained and this enzyme inhibition may act on active site. We found that ethanol extract of “Bang Chang” cultivar chili pepper yielded higher TPC content and more effective anti-lipase than oil extract. The finding provided benefits on applying this chili pepper on anti-lipase use and weight management.

Actual dietary intake versus dietary recommendation among patients with chronic kidney disease!-an observational study

Background: As diet is an important component of chronic kidney disease management. It is important to understand the actual dietary intake of these patients and see if they are able to meet their respective recommended dietary allowance (RDA), failing which they are predisposed to malnutrition. Hence the study was carried out to assess the actual dietary intake of the subjects with chronic kidney disease to understand the gap between recommendation and actual intake. Aim of this study was to assess the dietary intake of patients with chronic kidney disease. Methods: Patients with chronic kidney disease in stages 3 and 4 were recruited based on the inclusion criteria and the information on demographics, medical history, subjective data and seven-day dietary recall were obtained. The quantitative and qualitative estimation of the seven-day dietary intake was computed using “Dietcal” software. Results: Anorexia was observed among 54% of the study participants. The mean calorie, protein and carbohydrate intake was 959.69±166.49 Kcals, 27.94±5.36 g and 160.5±26.7 g respectively while mean intake of sodium, potassium and phosphorous were 88.65±42.88 mg, 944.5±188.69 mg and 560.77±127.64 mg respectively. Conclusions: The actual dietary intake was lesser than the recommended intake as influenced by anorexia, dysguesia, early satiety and dietary restrictions. Poor nutrient intake per se is the primary influential factor in the onset of malnutrition. Hence, it is important to adopt customized approach rather than generalized nutrient restrictions imposed upon these patients.

Effect of Recommended Dietary Intake versus Higher Doses of Supplemental Zinc on Iron and Copper Deficiency Anemia Among Patients with Chronic Kidney Diseases, A Double-Blinded, Randomized Clinical Trial

Background: Complex interplays happen in absorption and function of iron, zinc and copper. Both zinc deficiency and excess may lead to anemia. In Iran, commonly available supplements for chronic kidney disease (CKD) patients contain 25 mg-zinc (Zn). This study compared 25 mg versus 7.5 mg dose of zinc in anemia of CKD patients, the latter dose approximates to recommended dietary intake (RDI) of zinc. Methods: In this double-blinded clinical trial, 51 non-dialysis CKD patients were randomized to continue previous formulation (25 mg-Zn group) or change to a new preparation (7.5 mg-Zn group) for three months. Blood counts and serum iron, zinc and copper status were compared between and within the groups. Results: At the end of the study, serum copper and ceruloplasmin concentrations were significantly higher in 7.5 mg-Zn group compared with those in 25 mg-Zn arm (115.04± 23.05 vs. 102.48±14.98 µg/dL; P= 0.02 and 29.97±7.94 vs. 25.42±4.23 mg/dL; P= 0.01, respectively). Serum zinc levels did not differ between two groups (76.73±15.35 vs. 77.68±18.07 µg/dL for 7.5 mg-Zn and 25 mg-Zn groups, respectively; P= 0.84). After three months, patients in 7.5 mg-Zn group experienced increase in their Hb (11.11±1.17 vs. 10.72±1.03 g/dL; P= 0.04), HCT (35.28± 4.01 vs. 33.96± 3.74%; P= 0.03), MCV (86.30 (81.40-90.82) vs. 86.00 (80.35-88.77) ¦L; P= 0.01) and ferritin (202.60 (79.29-298.97) vs. 129.07 (42.25-225.87) ng/mL; P&lt;0.001) compared to their baseline values. Conclusion: Reducing zinc content to its RDI value in supplement for CKD patients led to increased serum copper and ceruloplasmin concentrations. Moreover, patients who switched to RDI zinc-containing formula experienced a significant rise in blood hemoglobin. hematocrit, mean corpuscular volume (MCV), and ferritin concentration.

Knowledge of Dietary Recommendations, Food Availability, and Food Selection Attitudes of Adults during the COVID-19 Pandemic

SARS-CoV-2, the novel coronavirus responsible for COVID-19 disease, has affected all regions of Indonesia, leading to an increase in cases and prompting the Indonesian government to impose Large-Scale Social Restrictions (LSSR). Prolonged exposure to LSSR can potentially impact diet, dietary availability, and food preferences. Therefore, understanding the knowledge of dietary recommendations during the COVID-19 pandemic is crucial to support individuals in maintaining a healthy diet and lifestyle. This study aims to investigate the relationship between knowledge of dietary intake recommendations, food availability, and food selection attitudes among adults during the COVID-19 pandemic. A cross-sectional design was employed, and 124 individuals were selected as research subjects using a purposive sampling technique. The data on knowledge, food availability, and attitudes towards food selection were collected through a Google Forms questionnaire. Statistical test was conducted using the Fisher Exact test to determine the relationship between variables. The findings of this study demonstrated no correlation between knowledge and food selection attitude (p&gt;0.05), as well as between food availability and food selection attitude (p&gt;0.05). However, there was a tendency for individuals with good knowledge to have good attitudes and individuals with good attitudes to have sufficient food availability, despite the absence of statistical correlation.

Assessments of energy, macro and micronutrient intakes in children and adolescents with type 1 diabetes mellitus

Objective: This study aims at examining dietary intake in children and adolescents with type 1 diabetes mellitus (DM) and comparing&#x0D; the results with national dietary intake recommendations.&#x0D; Patients and Methods: One hundred fifty children and adolescents (52.7% female) with an average age of 12.2±3.1 years and with&#x0D; type 1 DM who were followed by the Pediatric Endocrinology Polyclinic participated in the study. Three-day food intake records and&#x0D; clinical information regarding the type 1 DM condition of the participants were obtained.&#x0D; Results: No gender-related significant difference was found among the participants regarding food intake. The percentage of energy&#x0D; derived from fat (average 39.6%) and saturated fat (16.1%) were higher than the recommended levels in both gender groups. The&#x0D; percentage of energy derived from carbohydrates (female 44.1±5.7%, male 43.0±6.8%) was below the recommended levels. The dietary&#x0D; fiber intake in children aged 6-10 years with type 1 DM met recommendations, whereas, it was below the recommended levels in other&#x0D; age groups. Micronutrient inadequacy was common in children and adolescents with type 1 DM.&#x0D; Conclusions: The authors believe that guidelines and programs are needed for children and adolescents with type 1 DM to reduce&#x0D; total fat and saturated fat intake, increase carbohydrate and dietary fiber intake up to the recommended levels, and prevent multiple&#x0D; micronutrient inadequacies.

Novel ICP-OES-Based Method for the Reliable Determination of the Total Content of 15 Elements in Yerba Mate Drinks along with the Determination of Caffeine and the In Vitro Bioaccessibility of the Compounds.

A fully validated inductively coupled plasma optical emission spectrometry (ICP-OES)-based method combined with a simplified sample preparation procedure for the determination of up to 15 elements (Al, Ba, Ca, Cd, Cr, Cu, Fe, K, Mg, Mn, Na, Ni, Pb, Sr, and Zn) in caffeinated yerba mate (YM) drinks was proposed. Various "green" treatments (acidification or dilution with a HNO3 solution and direct analysis of untreated YM with or without sonication (US)) that could replace the traditional total sample decomposition before spectrometric measurements were tested and compared. The key selection parameter was the analytical performance of the ICP-OES method obtained with each sample preparation procedure in terms of the precision and the trueness of results and limits of detection (LODs) of elements. It was found that the acidification of YMs with concentrated HNO3 to 5%, supported by US (10 min, room temperature (RT)), provided the best results, i.e., LODs at 0.11-8.5 ng g-1, precision below 5%, and trueness better than 5% (97.0%-105% as recoveries). Eleven YM drinks, commercially available on the Polish market, were analyzed with the proposed method. In addition to the mineral content, the concentration of caffeine in all analyzed YMs was determined and compared. Finally, the studies were completed by determining the bioaccessible fraction of selected elements and caffeine in YMs using in vitro gastrointestinal digestion (GID) in order to evaluate the nutritional value/risk assessment of these drinks. Accordingly, the bioaccessibility of nutritious elements (Ca, Fe, Mg, Mn, Zn) and caffeine was within 40%-59%. Except for Mn, it was established that by drinking daily 1 L of YMs, the recommended dietary intakes (RDIs) of the aforementioned essential elements were covered to a low degree (<4.5%). Hence, they are not an important source of these elements in the human diet. On the other hand, potentially toxic elements (Al, Ba, Sr) were found in a relatively inert form. Opposite to minerals, YMs can supply human organisms with quite high amounts of natural caffeine in bioaccessible form (31-70 mg per serving).

Assessment of Mongolian dietary intake for planetary and human health.

Healthy diets that consider environmental pressures are required to meet sustainable development goals in Mongolia. This study aimed to clarify the extent of planetary and human health on Mongolian dietary intake. The intake of eight food groups (g/day) was investigated using the national database of the Household socio-economic survey (HSES) 2019 in Mongolia. The boundary intake of the Planetary health diet (PHD) proposed by the EAT-Lancet Commission was considered 100% adequate. The adequacy (%) of food groups in the HSES were calculated in two areas (urban and rural), during the two seasons (cold and warm), and the total by each boundary of the PHD. The differences between the recommended dietary intake (RDI) in Mongolia and the PHD were also investigated in the same manner. The adequacy of red meat (i.e., beef, mutton, and horsemeat) in whole areas of Mongolia indicated more than 17 times higher intake (1,738%) than the PHD. The adequacy of vegetables (20%) and fruits (8%) in Mongolia indicated an intake shortage compared to the PHD. These discrepancies in dietary adequacy were greater in rural areas and during the cold seasons than in urban areas and during the warm seasons, respectively. The animal-based protein sources, especially red meat (1,091%), in the RDI of Mongolia were higher than those in the PHD. This study found a highly excessive intake of red meat and a low intake of vegetables and fruits compared with the PHD among Mongolian people, especially in rural areas and during the cold seasons. The limited diversity of food in severe geographic conditions, poor accessibility of food retailers, and insufficient nutrition education may have led to these results. Therefore, improvements in the food environment and nutritional education are required.