Dietary Inclusion Rate Research Articles

Recirculating frass from food waste bioconversion using black soldier fly larvae: Impacts on process efficiency and product quality

Biowaste generation is increasing worldwide and inadequate disposal has strong negative impacts on food systems and ecosystems. Biodigestion of biowaste using black soldier fly (Hermetia illucens) larvae (BSFL) generates valuable by-products such as animal feed (larval biomass) and organic fertiliser (frass). However, the latter is typically unstable immediately after waste conversion and is thus unsafe for use as a fertilizer in terms of maturity. This study evaluated recirculation of frass within bioconversion of post-consumer food waste (FW) as a dietary component for BSFL to improve the quality of the subsequent frass obtained. Frass was introduced at increasing inclusion levels replacing food waste (2.5–100% on wet-weight basis) as part of the larvae's feeding substrate. Bioconversion efficiency and material reduction were significantly reduced by frass inclusion, while larval yield per experimental unit remained unchanged. When considering only the waste component in the larval diet, larval yield (dry-weight basis) ranged between 207 (0% frass inclusion) and 403 (40% frass inclusion) kg tonne FW−1, thus increasing by up to 94% at higher frass inclusion. With increasing dietary inclusion rate of frass from 0% to 100%, crude protein content of larval biomass increased by 41%, while fat content was reduced by 32%. The recirculated frass (obtained after including frass in the larval diet) had elevated concentrations of P, K, S, Na and B and around 6% lower organic matter content, demonstrating a higher degree of decomposition. Frass inclusion in the larval diet generated recirculated frass that were more stable and mature, as indicated by self-heating capacity, CO2 and NH3 volatilisation, seed germination bioassays and other parameters. It was concluded that frass recirculation improves waste bioconversion efficiency in relation to food waste unit, as well as larval biomass and frass quality, ensuring safer use as a fertilizer.

Amino acid and energy digestibility of conventional corn dried distillers grains with solubles, high protein distillers dried grains, and corn fermented protein, and impacts on turkey poult growth performance and intestinal characteristics

Amino acids (AA) are an expensive nutritional components of poultry diets. Distillers dried grains with solubles (DDGS) is the primary co-product produced by the dry grind bioethanol industry, although new technologies are being implemented to produce high protein distillers dried grains (HP-DDG) and corn fermented protein (CFP), but data on their nutritive value in poultry are lacking. Two experiments (EXP) were conducted to determine the energy and AA digestibility of DDGS, HP-DDG, and CFP in poults in addition to a feeding trial to evaluate increasing dietary levels of HP-DDG and CFP on growth performance and intestinal characteristics. In EXP 1, six different DDGS sources were evaluated using poults to determine their nitrogen-corrected apparent metabolizable energy (AMEn) concentrations, and cecectomized roosters were used to determine their standardized ileal (SID) AA digestibility (SID-AA). In EXP 2, AMEn and SID-AA for HP-DDG and CFP were determined in young poults, and a feeding trial was conducted to evaluate growth performance and intestinal morphology and permeability of poults fed diets containing 7.5 and 15% HP-DDG or CFP from 1 to 32 d of age. In EXP 1, the AMEn concentration among the DDGS samples ranged from 2,530 to 3,573 kcal/kg DM but was not different (P = 0.57) among the samples, with an average SID for LYS of 66.6%. In EXP 2, different (P = 0.001) AMEn concentrations for HP-DDG and CFP were observed (3,114 and 3,760 kcal/kg DM, respectively), with the SID for LYS being 66.55 and 77.00% for HP-DDG and CFP, respectively. Including HP-DDG or CFP into the diet at 7.5 and 15% had no effect (P > 0.05) on growth, feed intake, or feed conversion. Neither co-product nor its inclusion rate affected intestinal morphology and permeability (P > 0.05). Overall, DDGS, HP-DDG, and CFP are excellent sources of AMEn and digestible AA, with dietary inclusion rates of up to 15% of HP-DDG or CFP having no impact on growth or intestinal characteristics.

Hematological parameters of sheep and goats fed diets containing various amounts of water hyacinth (Eichhornia crassipes).

A major global barrier to increased animal output is nutrition. The use of aquatic plants, which were previously considered to be waste and needed a lot of labor to eliminate, has recently come to light due to the lack of feed during the dry season in the majority of tropical regions of Africa. The objectives of this study were therefore to see how different dietary Eichhornia crassipise inclusion rates affect the hematological indicators of Ethiopian Doyogena sheep and Woyto-Guji goats. Blood samples were taken from the jugular veins of 12 Doyogena sheep and 12 Woyto-Guji goats in a 2 × 4 randomized crossover design with two animal species, four diets, and four random periods (15 - day adaption period followed by a 7- day experimental diet in each period). The dietary inclusion rates E. crassipise were 0, 25, 50, and 75% that was used as a replacement for commercial concentrate mix diet in the treatment groups. The data was analyzed using the SAS software programme PROC GLM, and Pearson's correlation coefficient was calculated between hematological markers. The hemoglobin (Hb), red blood cell count (RBC), packed cell volume (PCV), mean corpuscular volume (MCH), and mean corpuscular hemoglobin (MCHC) results revealed substantial, RDW-SD, and WBC differences between animal species (P < 0.001). Sheep had greater WBC, Hb, RBC, PCV, RDW-SD, and RDW-CV levels, while goats had higher MCH and MCHC values (P < 0.001). For the analyzed hematological measures, the Pearson's correlation coefficient ranged from low to strong in terms of positive and negative associations (P < 0.05). Since all hematological indicators were closer to those of clinically healthy native Ethiopian sheep and goat breeds, feeding water hyacinth to sheep and goats up to a 75% inclusion level in diets without producing sickness may provide a remedy for adverse feed shortages.

The effects of cricket meal (Gryllus sigillatus) on the meat quality, growth, and internal morphology of broiler chickens

Cricket ( Gryllus sigillatus) meal (CM) was fed to Ross 308 broiler chickens ( n = 624 total; 26 birds/pen) at dietary inclusion rates of 0% (non-medicated control; NM), 0% (medicated control; 0.05% virginiamycin, 0.03% monensin; M), 5%, 10%, 15%, and 20% CM (non-medicated) for 35 days to determine the impact of this novel feed ingredient on the growth, feed intake, organ indices, and meat quality of these chickens. The final average live weight of broilers fed 5% CM was lower than broilers fed the 10% CM ( P &lt; 0.05) and the 0% NM diets ( P &lt; 0.05). Total weight gain was lowest in chickens fed 5% CM ( P &lt; 0.05) and the % ratio of the weight of the small intestine to body weight of the chickens fed 5% CM was significantly higher on day 20 than that of the other treatments ( P &lt; 0.05). Meat texture and colour were not affected by dietary treatments. Cooking loss in birds fed the 10% CM diet was significantly higher than that of birds fed the 0% NM control. Feeding up to 20% CM did not alter the growth, organ indices, or meat quality of broiler chickens, although further research is required to determine whether including &gt;20% CM in the diet will produce similar results.

Feeding limestone buffer to limit-fed dairy steers fed a high inclusion rate of distiller grains.

The study objective was to evaluate dietary limestone buffer inclusion rates for impacting nutrient digestibility and excretion when growing dairy cattle are fed high distiller grains with solubles (DDGS) limit-fed ration. The hypothesis was that feeding more limestone buffer would offset a low rumen pH when feeding a high DDGS inclusion rate. Five ruminally cannulated Holstein and Brown Swiss steers were used in a crossover design having 2-wk periods to evaluate high and low buffer inclusion rates when limit-fed. Treatments were similar in ingredient composition being: 1) 40% DDGS at 0.80% high CaCO3 buffer inclusion (HIGH) and 2) 40% DDGS with 0.25% low CaCO3 buffer inclusion (LOW) with the remaining ration consisting of grass hay with minerals and vitamins. Rations were limit-fed at 2.50% of body weight (BW) using Calan feeding doors with steers being weighed every 2wk with ration amounts adjusted accordingly. Rumen fluid was collected via the ruminal cannula at the start and end of each period for pH, ammonia-n, and volatile fatty acid (VFA)s. Fecal grab samples were collected at the end of each period for measurement of total-tract nutrient digestibility. There were no treatment-by-period interactions (P > 0.10) for any of the growth parameters measured. Gains in frame growth parameters were similar (P > 0.10) for steers fed both rations. BWs and gains were similar (P > 0.10) for steers fed both rations. Steers fed the LOW ration demonstrated a numeric improvement in average daily gain combined with a tendency (P < 0.06) for lower dry matter intake resulting in an improved (P < 0.01) feed efficiency (gain:feed) compared with steers fed the HIGH ration. Steers fed HIGH demonstrated greater (P < 0.01) ruminal ammonia-N and isovalerate concentrations compared with steers fed the LOW buffer ration. Steers fed both buffer inclusion rates were similar (P > 0.10) for ruminal pH and remaining VFAs concentrations. Steers fed both buffer inclusion rates were similar (P > 0.10) in DM and organic matter digestibilities. Limit-feeding a high DDGS inclusion rate ratio combined with a low buffer inclusion rate improved feed efficiency while maintaining growth performance. The study hypothesis was rejected in that feed efficiency can be enhanced when feeding a high DDGS ration by feeding a low calcium carbonate buffer.

Jerusalem Artichoke (Helianthus tuberosus L.) as a Promising Dietary Feed Ingredient for Monogastric Farm Animals

In recent years, there has been significant attention toward the incorporation of alternative functional feed ingredients in monogastric diets. The objective is to improve sustainability and optimize animal performance both under normal conditions and in heat stress situations. Among these alternatives, Jerusalem artichoke (Helianthus tuberosus L.) has emerged as a promising candidate due to its nutritional composition and potential health benefits. This review aims to investigate the potential utilization of Jerusalem artichoke in monogastric diets and the impact on productive performance parameters. Moreover, the potential prebiotic effects of Jerusalem artichoke on the composition and activity of monogastric gut microbiota are revealed, showing its implications for gut health and reduction in pathogenic bacteria. The incorporation of Jerusalem artichoke in monogastric diets poses several challenges, such as limitation of the dietary inclusion rate. However, there are also future perspectives to consider, such as optimizing processing techniques, evaluating the effects of different cultivars, and exploring potential synergies with other dietary feed ingredients. In summary, this study provides a comprehensive overview of the key findings and unique perspectives on the utilization of Jerusalem artichoke in monogastric diets, highlighting its potential as a valuable feed ingredient.

Quality and safety of hemp meal as a protein supplement for nonlactating dairy cows

Hemp seed meal may be a suitable protein supplement for dairy cows, but its quality and safety as a dairy cow feed has not yet been fully investigated. As a result, dry matter intake (DMI), rumen fermentation, blood metabolites, total-tract digestibility, and concentrations of cannabinoids in blood plasma, urine, muscle, and adipose tissues were compared among nonlactating Holstein dairy cows receiving a basal partial mixed ration that was supplemented with either 10.2% dry matter (DM) hemp meal (HM treatment), 13.5% DM canola meal (CM treatment), or 6.25% DM hemp meal and 6.16% DM canola meal (HC treatment). Diets were formulated to be isoenergetic and isonitrogenous. Six nonlactating, nonpregnant Holstein cows were used in a repeated 3 × 3 Latin square design trial with three 3-wk experimental periods. The first 2 weeks of each served as adaptation. Sample and data collection occurred during the third week of each period. Neither the partial mixed ration nor canola meal contain cannabidiol (CBD), cannabidiolic acid (CBDA), d9-tetrahydrocannabinol (THC), or tetrahydrocannabinolic acid A (THCA-A). However, the hemp meal contained 3.0, 4.4, 0, and 0.1 μg/g DM of CBD, CBDA, THC, and THCA-A, respectively. Treatment did not affect DMI, pH, concentrations of volatile fatty acids or ammonia in the rumen, total-tract digestibilities of DM and crude protein, or blood plasma concentrations of glucose, urea, β-hydroxybutyrate, and nonesterified fatty acids. Hence, based on these metabolites, treatment did not affect the nutritional status of the cows. However, the total-tract neutral detergent fiber digestibility of the CM treatment (43%) was higher than that of the HM treatment (38%). No cannabinoids were detected in blood plasma, rumen fluid, and urine. Cannabinoids were also not detected in kidney, liver, urine, muscle, or adipose tissues at the end of the experiment when cows had undergone all treatments. Feces from all treatments did not contain detectable concentrations of THC or THCA-A, but feces of cows on the HC treatment contained 0.42 and 0.40 μg/g DM of CBD and CBDA, respectively. Feces of cows on the HM treatment contained 0.68 and 0.67 μg/g DM of CBD and CBDA, respectively. This indicated that most ingested CBD and CBDA were not absorbed but instead were excreted in the feces. Our data show dietary inclusion rates of up to 10.2% of DM. We find that hemp meal is a high-quality and safe protein supplement for nonlactating dairy cows.

Growth performance and quality indicators of European seabass (Dicentrarchus labrax) fed diets including refined glycerol

Glycerol is a by-product of the biodiesel industry with growing availability and applicability in animal feeds. However, its use as a source of energy in aquafeeds has only been investigated in a small number of fish species. The effects of dietary refined glycerol inclusion rate on growth performance, nitrogen and energy balances, and fish quality were investigated in juvenile European seabass. Twelve fish were assigned to each of the nine tanks and used to test the experimental diets in triplicate. The diets comprised 0, 25, or 50 g kg−1 of refined glycerol (G0, G25, and G50, respectively), being not isoenergetic, although having similar digestible protein-to-energy ratios (22 mg kJ−1). Fish were fed twice a day to apparent satiation for 90 days. The G25 group displayed a higher absolute feed intake and a higher weight gain than the G0 or G50 groups, although feed efficiency and the use of protein as an energy source were similar between the three groups. Seabass fed the G25 diet also displayed a higher specific growth rate than fish fed the G50 diet. Higher hepato-somatic and visceral lipid-somatic indices of seabass were observed with increased levels of dietary glycerol, although proximal body composition remained similar across groups. The apparent digestibility coefficients for dry matter, crude protein, and energy increased with increasing dietary glycerol inclusion rate, whereas the apparent lipid digestibility coefficient was highest for the G25 group. No differences in the energy retained as protein, nitrogen efficiency, and total ammonia excretion rate were detected between seabass fed the different diets. At the end of the growth trial, physical and microbiological quality parameters of the skin and gill surfaces were assessed after 1 and 10 days of storage on ice. Fish quality decreased with storage time but was not affected by the dietary glycerol inclusion rate. In summary, the inclusion of refined glycerol in seabass diets may have economic benefits for feed manufacture and a positive effect on growth performance but does not change the efficiency of protein utilization in this carnivorous species.

The Effect of Diets Containing High-Moisture Corn or Triticale Grain on Animal Performance and the Fatty Acid Composition of Lamb Muscles.

The aim of this study was to evaluate the effect of diets with different inclusion levels of high-moisture corn (HMC) and triticale grain (TG) on nutrient digestibility and utilization, the growth performance of lambs, and the fatty acid profile of the leg muscle. The experiment was performed on 24 young rams, divided into four groups based on grain type and inclusion rate (50% or 75% DM). The higher inclusion rate of cereal grain and a lower concentration of crude fiber (CF) in the feed ration decreased CF digestibility by 26% and 35% in diets containing HMC and TG, respectively. Diets containing HMC increased crude fat digestibility relative to animals receiving TG. Final body weight and average daily gain were highest in lambs fed a diet containing 50% HMC. Grain type had no effect on nitrogen (N) retention or the proportions of SFA and UFA in lamb meat. Diets containing HMC decreased total cholesterol levels in the leg muscle. HMC can be a valuable component of diets for growing lambs when included at up to 50% of the ration. An increase in the dietary inclusion rate of cereal grain to 75% can be recommended only in the case of TG.

Phytase dose-dependent response of kidney inositol phosphate levels in poultry.

Phytases, enzymes that degrade phytate present in feedstuffs, are widely added to the diets of monogastric animals. Many studies have correlated phytase addition with improved animal productivity and a subset of these have sought to correlate animal performance with phytase-mediated generation of inositol phosphates in different parts of the gastro-intestinal tract or with release of inositol or of phosphate, the absorbable products of phytate degradation. Remarkably, the effect of dietary phytase on tissue inositol phosphates has not been studied. The objective of this study was to determine effect of phytase supplementation on liver and kidney myo-inositol and myo-inositol phosphates in broiler chickens. For this, methods were developed to measure inositol phosphates in chicken tissues. The study comprised wheat/soy-based diets containing one of three levels of phytase (0, 500 and 6,000 FTU/kg of modified E. coli 6-phytase). Diets were provided to broilers for 21 D and on day 21 digesta were collected from the gizzard and ileum. Liver and kidney tissue were harvested. Myo-inositol and inositol phosphates were measured in diet, digesta, liver and kidney. Gizzard and ileal content inositol was increased progressively, and total inositol phosphates reduced progressively, by phytase supplementation. The predominant higher inositol phosphates detected in tissues, D-and/or L-Ins(3,4,5,6)P4 and Ins(1,3,4,5,6)P5, differed from those (D-and/or L-Ins(1,2,3,4)P4, D-and/or L-Ins(1,2,5,6)P4, Ins(1,2,3,4,6)P5, D-and/or L-Ins(1,2,3,4,5)P5 and D-and/or L-Ins(1,2,4,5,6)P5) generated from phytate (InsP6) degradation by E. coli 6-phytase or endogenous feed phytase, suggesting tissue inositol phosphates are not the result of direct absorption. Kidney inositol phosphates were reduced progressively by phytase supplementation. These data suggest that tissue inositol phosphate concentrations can be influenced by dietary phytase inclusion rate and that such effects are tissue specific, though the consequences for physiology of such changes have yet to be elucidated.

Effect of inclusion of bakery by-products in the dairy cow's diet on milk fatty acid composition.

Bakery by-products (BP), rich in fats and sugars, are unconventional feed sources for cows whose effects on milk fat composition have not yet been evaluated. This research paper aimed to assess the effects of dietary BP inclusion rate and feeding period on the milk fatty acid composition. Twenty-four Simmental cows were fed a diet without BP (CON) for 1 week. Then they either continued with the CON diet or switched to one of the BP diets (with 15% or 30% BP in diet dry matter) for 3 weeks. Milk samples were taken before diet change and three times during BP feeding and analysed for fatty acid composition. Data showed that increasing BP content in the diet increased total fatty acid intake, especially of 18 : 1 n9. In the milk fat, the percentages of total monounsaturated fatty acids especially of the 18 : 1 origin linearly increased with increasing dietary BP level. The percentage of fatty acids de novo synthesized in the mammary gland (the sum of 4 : 0-14 : 0) remained similar among diets (32-34% of total fatty acids). The 16 : 0 percentage dropped from 32.5 to 29.6% and from 33.6 to 28.3% for 15% and 30% BP, respectively. Only 30% BP elevated the percentage of conjugated linoleic acids (CLA: by 59%) compared with CON throughout the 3 weeks. Proportions of 18 : 2 n6 and 18 : 3 n3 and the n6:n3 ratio were unaffected by BP and feeding time. BP feeding improved all those estimated health indices of the milk fat that are suggested to be related to coronary health. In summary, the inclusion of BP in dairy rations beneficially shifted the milk fatty acid profile to more 18 : 1 fatty acids at the expense of 16 : 0. At a 30% inclusion rate, BP feeding showed an additional benefit of increased CLA content in milk fat.

Chemical Composition and Fatty Acid Profile of the Pectoralis major Muscle in Broiler Chickens Fed Diets with Full-Fat Black Soldier Fly (Hermetia illucens) Larvae Meal.

Simple SummaryInsect meal (processed insect protein) can effectively cater to the growing demand for protein sources in animal diets. However, insect meals have to be thoroughly analyzed before they can be incorporated into the diets of monogastric animals (such as poultry) because animal nutrition is directly correlated with the quality (including chemical composition) of meat, which was confirmed by the present study. Further research is needed to determine the efficacy of full-fat Hermetia illucens (HI) larvae meal in broiler chicken nutrition. Lower dietary inclusion levels of insect meal than those analyzed in this study, and insect meal with a modified fatty acid profile should be investigated to optimize the nutritional value and health benefits of poultry meat.The aim of this study was to determine the effect of full-fat Hermetia illucens (HI) larvae meal used as a substitute for 50%, 75% and 100% of soybean meal (SBM) in diets fed to male Ross 308 broiler chickens on the proximate chemical composition and fatty acid profile of the Pectoralis major (PM) muscle. The muscles of chickens fed HI larvae meal were characterized by a higher (p < 0.05) total concentration of pigments and lower (p < 0.05) ash content. At the lowest dietary inclusion rate (50%) of HI larvae meal, the PM muscle had a lower (p < 0.05) content of fat and collagen, compared with the remaining treatments. An analysis of the fatty acid profile of the PM muscle revealed that the total concentrations of saturated fatty acids increased (p < 0.05) and the total concentrations of polyunsaturated fatty acids decreased (p < 0.05) with increasing inclusion levels of HI larvae meal. The changes in the fatty acid profile of muscles in birds receiving HI larvae meal led to a decrease in the nutritional value of fat. The present findings indicate that the ≥50% inclusion rate of full-fat HI larvae meal as a protein source alternative to SBM in broiler chicken diets is too high due to its negative effect on the fatty acid profile of meat.

Impact of Dietary Sodium Butyrate and Salinomycin on Performance and Intestinal Microbiota in a Broiler Gut Leakage Model.

Simple SummaryImbalanced microbiota related to intestinal leakage is an emerging health problem in commercial broilers associated with partial loss of gut function and reduction in growth performance. Salinomycin is an ionophore coccidiostat with antibiotic effect, which is used as additive in broiler diets to control enteric diseases and improve performance. Due to the growing concern of the development of bacterial antibiotic resistance, ionophores are expected to be banned, within the EU, as feed additives in the near future. Butyrate with multiple beneficial effects on growth performance and pathogen control in broilers has been introduced as a promising alternative. In this study, the ability of a coated butyrate product to alleviate intestinal imbalance was compared to that of salinomycin after enteric challenge. Compared to butyrate and non-supplemented control, salinomycin increased potentially beneficial Ruminococcaceae and reduced potentially pathogenic Enterobacteriaceae, and counts of Lactobacillus salivarius and Clostridium perfringens. Further, salinomycin improved broiler performance. Dietary inclusion of coated butyrate had only limited effects on the composition of the broiler microbiota. Whether improved growth and feed utilization in the salinomycin-supplemented animals can be explained by suppression of C. perfringens and L. salivarius and enrichment of butyrate- and lactic acid-producing bacteria (Ruminococcaceae and Lactobacillaceae) needs further verification.Unfavorable alterations of the commensal gut microbiota and dysbacteriosis is a major health problem in the poultry industry. Understanding how dietary intervention alters the microbial ecology of broiler chickens is important for prevention strategies. A trial was conducted with 672 Ross 308 day-old male broilers fed a basic diet (no additives, control) or the basic diet supplemented with 500 mg/kg encapsulated butyrate or 68 mg/kg salinomycin. Enteric challenge was induced by inclusion of 50 g/kg rye in a grower diet and oral gavage of a 10 times overdose of a vaccine against coccidiosis. Compared to control and butyrate-supplemented birds, salinomycin supplementation alleviated growth depression. Compared to butyrate and non-supplemented control, salinomycin increased potentially beneficial Ruminococcaceae and reduced potentially pathogenic Enterobacteriaceae and counts of Lactobacillus salivarius and Clostridium perfringens. Further, salinomycin supplementation was accompanied by a pH decrease and succinic acid increase in ceca, while coated butyrate (0.5 g/kg) showed no or limited effects. Salinomycin alleviated growth depression and maintained intestinal homeostasis in the challenged broilers, while butyrate in the tested concentration showed limited effects. Thus, further investigations are required to identify optimal dietary inclusion rates for butyrate used as alternative to ionophore coccidiostats in broiler production.

Effects of feeding diets containing increasing content of purified lignocellulose supplied by sugarcane bagasse to early-weaned pigs on growth performance and intestinal health

A total of 96 weaned piglets, with mean age of 21 days and initial body weight (BW) of 6.67 ± 0.63 kg, were used in a 42-day experiment to evaluate the effects of supplementation of dietary purified lignocellulose (LNC) on growth performance and intestinal health. Piglets were blocked by initial BW and allotted to one of 32 pens, each pen containing three pigs. Dietary treatments consisted of a corn-soybean meal-based control diet with no added LNC and three dietary inclusion rates (10, 20, and 30 g/kg) of LNC. Average daily feed intake (ADFI), average daily gain (ADG), and gain-to-feed ratio (G:F) were determined over 42 days postweaning. On day 14 postweaning, one pig from each pen was euthanized to determine gastrointestinal tract organ weight, small intestinal morphology, and digesta pH, viscosity, fermentation end-products, and bacterial enumeration. Total tract retention time was measured by adding 10 g/kg ferric oxide in the morning feed on day 42. From day 1–14 postweaning, G:F tended to linearly increase (P = 0.088) as the dietary LNC contents increased. Although increasing the dietary LNC supplementation quadratically increased (P = 0.008) ADFI and tended to quadratically decrease (P = 0.072) G:F from day 30–42 postweaning, overall growth performance was unaffected by the treatments. The weight of the small intestine adjusted for kg of BW on day 14 postweaning increased quadratically (P = 0.041) as the dietary LNC contents increased. Increasing the dietary LNC supplementation linearly increased (P = 0.027) crypt depth and tended to linearly reduce (P = 0.075) villus:crypt ratio in the jejunum. Dietary treatments did not affect total tract retention time as well as pH and viscosity of both cecum and colonic digesta. Increasing the dietary LNC supplementation quadratically increased (P = 0.001) cecal butyrate formation, whereas cecal acetate and propionate concentrations did not differ among treatments. Although ileal counts of Lactobacillus spp. and Escherichia coli were similar among treatments, the pathogenic Clostridium perfringens population exhibited a quadratic decrease (P = 0.041) as the dietary LNC contents increased. These results indicate that feeding pig diets containing up to 30 g/kg purified LNC enhanced gut health through the inhibition of harmful bacteria proliferation and the expansion of butyrate producers, without compromising overall growth performance.

Meta-analysis of the effects of dietary inclusion of sericea lespedeza (Lespedeza cuneata) forage on performance, digestibility, and rumen fermentation of small ruminants

For more than 20 years, the nutraceutical forage sericea lespedeza ‘AU-grazer’ (SL; Lespedeza cuneata) has been used as an alternative to anthelmintic drugs for controlling gastrointestinal parasites in small ruminants, but with little attention to animal performance. Thus, the objective of this study was to evaluate the effects of dietary inclusion of SL on performance, digestibility, and rumen fermentation of small ruminants using a meta-analytic approach. A total of 24 peer-reviewed manuscripts (sheep = 6 and goats = 18) from 2000 to 2020 met the inclusion criteria for the analysis. Raw mean differences between dietary SL and control treatments weighted by inversed variance were compared with a robust variance estimation. Diet characteristics like crude protein content, NDF content, condensed tannin (CT) content, and inclusion level of SL in the diet (0% to 100%) were used as covariates in a metaregression and subgrouping analysis. Compared to the control, dietary inclusion of SL decreased (P < 0.05) total-tract digestibility by 12% (DMD) and average daily gain (ADG) by 17.7%, but no effects were observed in dry matter intake. Likewise, dietary SL reduced (P < 0.05) molar proportion of acetate (68.88 vs 71.8%), but increased butyrate (10.3 vs 9.2%) and propionate (17.9 vs 16.8%) molar proportions in the rumen. Consequently, dietary SL reduced CH4 emissions by 38% compared to the control. Despite the high heterogeneity in the response variables (I2 > 50), no significant bias was detected (funnel test; P > 0.05). The dose-response analysis revealed that SL-based diets should not include more than 60% in the diet to prevent negative effects in DMD and ADG. Likewise, low inclusion level of SL in the diet (from 30 to 40%) linearly increased propionate concentrations in the rumen but reduced CH4 emissions. In addition to the nutraceutical benefits of this forage, SL inclusion improved rumen fermentation (increased propionate, reduced CH4) at low rates of inclusion, but reduced ADG in small ruminants at higher rates, with these effects minimized if dietary inclusion rate for SL is 60% or less. Further research is needed to determine the optimum inclusion rate to balance nutritional and pharmaceutical effects of SL in the diet of small ruminants.

Effect of nucleotides on growth performance, gut health, and some immunological parameters of broiler chicken exposed to high stocking density

The current study was conducted to investigate the effect of dietary nucleotides inclusion rate on growth performance, gut health, and immunity of broiler chickens exposed to high stocking density-induced stress. A total of 315 one-day-old mixed broiler chickens were randomly assigned to a 2 × 3 factorial arrangement of treatments with 2 different stocking densities, low stocking density (LSD; 0.073 m2/2.2 kg broiler chicken; 15 broiler chickens/replicate) and high stocking density (HSD; 0.05 m2/2.2 kg broiler chicken; 20 broiler chickens/replicate), and 3 dietary inclusion rates of nucleotides at 0, 0.05, and 0.1%, with 3 cages per treatment. The experiment lasted for 35 days. At the end of the experiment, intestinal histomorphology, digestive enzymes production, immune status, and growth performance of broiler chickens were determined. The results showed that intestinal histomorphological picture, immune indices, and digestive enzymes production were negatively affected (P < 0.05) with HSD, resulting in a decreased (P < 0.001) body weight gain (BWG), feed intake (FI) (P < 0.001), and an increased (P < 0.001) feed conversion ratio (FCR). While the intestinal and pancreatic enzymes production and intestinal histomorphology, including villus height, mucosal thickness, and goblet cell number increased linearly (P < 0.001) with the increased nucleotides supplementation rate, which resulted in a linear increase in BWG (P < 0.001) and FI (P < 0.001), and a decrease in FCR (P = 0.026). However, the effect was more prominent at LSD than HSD. In addition, immune indices, including antibody against NDV, IL-2 and INF-γ gene expression, and lysozyme production, linearly increased (P < 0.001) with the increase of dietary nucleotides inclusion rate, resulting in partial mitigation of immune compromise in broiler chickens at HSD. We concluded that dietary nucleotide supplementation, particularly at 0.1%, partially attenuated the negative effect of HSD on broiler chicken performance through improving intestinal histomorphology, digestive enzyme production, and immune status.

Effects of increasing dietary inclusion of camelina cake on growth performance of growing-finishing pigs.

The objective of this experiment was to determine the dietary inclusion rate of camelina cake (CC) that would support the growth performance of growing-finishing pigs similar to that of a corn-soybean meal-based diet. Pigs (n = 192; BW = 35.2 kg; Duroc x (Yorkshire x Landrace)), balanced for sex and initial weight, were assigned to pens (8 pigs/pen) and pens were assigned randomly to one of four dietary treatments (6 pens/treatment). Treatments consisted of a non GMO corn-soybean meal control diet (CON), or CON containing 5% (5CC), 10% (10CC), or 15% (15CC) camelina cake. Feed disappearance on a pen basis and individual body weights of pigs were recorded every other week to calculate average daily gain (ADG), average daily feed intake (ADFI), and gain to feed ratio (G:F) on a pen basis. Prior to harvest, real-time ultrasonic measurements of back fat depth and loin eye area were collected on all live pigs. Pigs were harvested as a single group at about 23 weeks of age at a commercial abattoir. Data were analyzed using Proc Glimmix with dietary treatment as a fixed effect and pen serving as the experimental unit. Growth performance data collected over time were analyzed using repeated measures within the Proc Glimmix procedure. Overall, pigs fed CON exhibited similar ADG to those consuming 5CC and higher ADG than pigs consuming 10CC and 15CC diets (1.10 kg vs. 1.05 kg for 10CC and 1.02 kg for 15CC; P < 0.05 for both mean comparisons). Pigs fed CON consumed more feed than pigs fed any of the CC diets (ADFI = 2.66 kg for CON vs. 2.46 kg for 5CC, 2.46 kg for 10CC and 2.47 kg for 15CC; P < 0.05 for all). These differences resulted in heavier (P < 0.05) CON-fed pigs at marketing than 10CC or 15CC-fed pigs. There were no differences in any carcass traits analyzed. From these data, we conclude that feeding up to 5% CC in corn-soybean meal-based diets did not negatively influence growth performance, or carcass traits of growing-finishing pigs.

Bioavailability of the dl-methionine and the calcium salt of dl-methionine hydroxy analog compared with l-methionine for nitrogen retention in starter pigs.

Two nitrogen balance studies were conducted to evaluate the relative bioavailability values (RBV) of dl-methionine (dl-Met) and dl-methionine hydroxy analog calcium salt (MHA-Ca) to l-methionine (l-Met) as Met sources fed to pigs. In experiment 1, 42 pigs were assigned to 7 treatments feeding with basal diet (BD) formulated to be deficient in Met (0.22% standardized ileal digestible basis) but adequate in other amino acids. Diets included (1) BD, (2) BD + 0.025% dl-Met, (3) BD + 0.050% dl-Met, (4) BD + 0.075% dl-Met, (5) BD + 0.025% l-Met, (6) BD + 0.050% l-Met, and (7) BD + 0.075% l-Met. Increasing levels of l-Met and dl-Met enhanced N retained (g/d) and N retention (% of intake) linearly (P < 0.01). Using a linear slope ratio procedure, a product-to-product RBV of dl-Met compared with l-Met was 94% (95% confidence limits: 65% to 123%) based on N retained expressed as g/d and 99% (95% confidence limits: 70% to 128%) for N retention expressed as % of intake. In experiment 2, 42 pigs were allotted to 7 treatments in another N-balance trial. Diets included (1) BD, (2) BD + 0.025% l-Met, (3) BD + 0.050% l-Met, (4) BD + 0.075% l-Met, (5) BD + 0.030% MHA-Ca, (6) BD + 0.060% MHA-Ca, and (7) BD + 0.089% MHA-Ca. An increase in dietary inclusion rates of l-Met increased (P < 0.01) N retained (g/d) linearly while increasing levels of MHA-Ca had no effects (P > 0.05) on N retained (g/d) and N retention (% of intake). Using linear slope-ratio regression, the RBV of MHA-Ca compared with l-Met was 70% (95% confidence limits: 59% to 81%) on a product-to-product basis or 83% on equimolar basis based on N retained expressed as g/d. Overall, the mean RBV of dl-Met to l-Met of 97% (95% confidence limits cover 100%) indicated that dl-Met and l-Met are equally bioavailable as Met sources in pigs. Compared with l-Met, the RBV of MHA-Ca was lower at 70% (95% confidence limits: 59% to 81%) on a product-to-product basis or 83% on equimolar basis in starter pigs.

Dietary inclusion of AZOMITE improves feed efficiency in broilers and egg production in laying and broiler breeder hens

The dietary inclusion of aluminosilicates has been reported to enhance pellet quality, improve feed mill throughput, bind toxins, improve feed efficiency, and promote immunological function across a variety of production systems. AZOMITE is a product marketed as a hydrated sodium calcium aluminosilicate containing macro and trace minerals, and rare earth elements and the potential benefits of its dietary inclusion in broiler, layer, and broiler breeder diets was investigated. In a battery study, broilers were fed diets containing 0, 0.125, 0.250, or 0.500% AZOMITE from 0 to 21 d of age. Laying hens were fed a control diet or this diet supplemented with 0.25% AZOMITE from 54 through 98 wk of age, with the hens fed a standard molting diet or this diet supplemented with 0.25% AZOMITE from 71 to 72 wk of age. Broiler breeder hens were fed a control diet or this diet supplemented with 0.25% AZOMITE from the onset of photostimulation at 21 wk of age through 65 wk of age. All 3 dietary inclusion rates of AZOMITE improved (P < 0.05) the feed to body weight gain ratio in broilers fed these diets relative to broilers fed the control diet. In laying hens total marketable eggs, and in broiler breeder hens total settable eggs were increased (P < 0.05) with the dietary inclusion of AZOMITE by 8 eggs per hen. The inclusion of dietary AZOMITE also improved apparent Ca and P digestibility in broilers and tibia ash content in laying hens. The results indicate the dietary inclusion of AZOMITE in poultry diets improves bird performance.

Does the length of time dried distillers' grain with solubles substitution for soybean meal affect physiological indicators and meat quality in finishing lambs?

This study examined how inclusion of dried distillers' grains with solubles (DDGS) in finishing lamb diets for different periods affects some physiological indicators and meat quality. A total of 40 male lambs were divided into four groups according to feeding regimen during the 120-day finishing period as follows: C120: no DDGS included in diet for 120days; D120: DDGS included in diet for 120days; D75: no DDGS included in diet for 45days+DDGS included in diet for 75days; D45: no DDGS included in diet for 75days+DDGS included in diet for 45days. Dietary inclusion rate of DDGS was 27.5%. Fattening performance and rumen parameters were not affected by treatment. Feeding regimens had no significant effect on meat quality except the instrumental tenderness and juiciness score. DDGS fed lambs had higher level of total trans fatty acids and n-6/n-3 ratio in meat. 10t-C18:1, 11t-C18:1 and 9c,11t conjugated linoleic acid contents of adipose tissue were higher in all the DDGS groups compared with C120 lambs (p<.001). These results suggest that soybean meal can be replaced with corn DDGS in lamb diets for up to 120days during the finishing period with no adverse effects on some physiological response feedlot performance and meat quality.