Mammals may experience physical changes from birth, and their diet varies at different stages of life. This study investigates the impact of development on the diet composition of three horseshoe bats: Rhinolophus euryale, R. hipposideros, and R. ferrumequinum in the Basque Country, north of the Iberian Peninsula. The diets of juvenile and adult individuals of each species were obtained by analysing their droppings using metabarcoding and then compared at (1) the taxonomic and (2) prey trait levels (size, flying speed, hardness). The diets of juvenile and adult individuals of R. euryale and R. hipposideros showed significant differences at the taxonomic level and regarding prey traits. In contrast, in the case of R. ferrumequinum, we could only observe discernible diet patterns through the trait analysis. Additionally, we discovered a shared pattern: younger individuals tend to feed on easier-to-hunt and/or handle smaller and smoother prey. The varying degrees of dissimilarity between juvenile and adult diets observed in this study suggest that the relative importance of psychomotor development, foraging strategies, prey discrimination, and/or spatial learning may differ among species. These findings contribute to conservation efforts, especially by recognising the dietary needs of juveniles for their survival and successful development.
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