Diencephalic Cell Groups Research Articles

Localization of putative glutamatergic/aspartatergic neurons projecting to the supraoptic nucleus area of the rat hypothalamus.

Oxytocin and vasopressin neurosecretory neurons of the supraoptic nucleus receive a rich glutamatergic innervation. The nerve cells of this prominent structure express various ionotropic and metabotropic glutamate receptor subtypes and there is converging evidence that glutamate acts as an excitatory transmitter in the control of release of oxytocin and vasopressin synthesized in this cell group. The location of the glutamatergic neurons projecting to this hypothalamic region is unknown. The aim of the present investigation was to study this question. [(3)H]D-aspartate, which is selectively taken up by high-affinity uptake sites at presynaptic endings that use glutamate as a transmitter, and is transported back to the cell body, was injected into the supraoptic nucleus area. The neurons retrogradely labelled with [(3)H]D-aspartate were detected autoradiographically. Labelled nerve cells were found in several diencephalic and telencephalic structures, but not in the brainstem. Diencephalic cell groups included the supraoptic nucleus itself, its perinuclear area, hypothalamic paraventricular, suprachiasmatic, ventromedial, dorsomedial, ventral premammillary, supramammillary and thalamic paraventricular nuclei. Within the telencephalon, labelled neurons were detected in the septum, amygdala, bed nucleus of the stria terminalis and preoptic area. The findings provide neuromorphological data on the location of putative glutamatergic neurons projecting to the supraoptic nucleus and its perinuclear area. Furthermore, they indicate that local putative glutamatergic neurons as well as several diencephalic and telencephalic structures contribute to the glutamatergic innervation of the cell group and thus are involved in the control of oxytocin and vasopressin release by neurosecretory neurons of the nucleus.

Identification of the Anterior Nucleus of the Ansa Lenticularis in Birds as the Homolog of the Mammalian Subthalamic Nucleus

In mammals, the subthalamic nucleus (STN) is a glutamatergic diencephalic cell group that develops in the caudal hypothalamus and migrates to a position above the cerebral peduncle. By its input from the external pallidal segment and projection to the internal pallidal segment, STN plays a critical role in basal ganglia functions. Although the basal ganglia in birds is well developed, possesses the same major neuron types as in mammals, and plays a role in movement control similar to that in mammals, it has been uncertain whether birds possess an STN. We report here evidence indicating that the so-called anterior nucleus of the ansa lenticularis (ALa) is the avian homolog of mammalian STN. First, the avian ALa too develops within the mammillary hypothalamic area and migrates to a position adjacent to the cerebral peduncle. Second, ALa specifically receives input from dorsal pallidal neurons that receive input from enkephalinergic striatal neurons, as is true of STN. Third, ALa projects back to avian dorsal pallidum, as also the case for STN in mammals. Fourth, the neurons of ALa contain glutamate, and the target neurons of ALa in dorsal pallidum possess AMPA-type glutamate receptor profiles resembling those of mammalian pallidal neurons. Fifth, unilateral lesions of ALa yield behavioral disturbances and movement asymmetries resembling those observed in mammals after STN lesions. These various findings indicate that ALa is the avian STN, and they suggest that the output circuitry of the basal ganglia for motor control is similar in birds and mammals.

Neurons of the posterior subdivision of the nucleus preopticus periventricularis project to the preglomerular nucleus in the weakly electric fish, Apteronotus leptorhynchus

By using an in vitro tract-tracing technique, the neural connections between two diencephalic cell groups, the posterior subdivision of the nucleus preopticus periventricularis (PPp) and the preglomerular nucleus (PG), was examined in the weakly electric gymnotiform fish Apteronotus leptorhynchus. Neurons of the PPp project to one area within PG, the ventromedial cell group of the medial subdivision of the preglomerular nucleus (PGm-vmc). Axons of these cells reach the ipsilateral PGm-vmc via the basic hypothalamic tract, while collaterals decussate via the postoptic commissure to innervate the contralateral PGm-vmc. We hypothesize that those neurons within PPp that project to the PGm-vmc are homologous to neurons of the medial preoptic area of mammals. As part of an elaborate circuit, PPp and PG may participate, as in mammals, in the control of complex social behavior patterns.

Reciprocal connections between the preglomerular nucleus and the central posterior/prepacemaker nucleus in the diencephalon of weakly electric fish, Apteronotus leptorhynchus

The central posterior/prepacemaker nucleus of gymnotiform fish is a bilateral cell group located in the dorsal thalamus. This complex consists of approximately 10,000 neurons which can be divided into several subpopulations. One subpopulation comprised of a few hundreds of neurons projects to the pacemaker nucleus in the medulla oblongata, thus constituting the prepacemaker nucleus portion of this complex. By employing in vitro tract-tracing techniques, we have, in the present investigation, examined the pattern of connectivity formed by the central posterior/prepacemaker nucleus with a diencephalic cell group, the preglomerular nucleus. As demonstrated by anterograde and retrograde tracing, a subpopulation of several hundreds of neurons located in the central posterior/prepacemaker nucleus project to the ipsi- and contralateral preglomerular nucleus. Double-labelling experiments revealed that at least a fraction of these neurons also innervate the pacemaker nucleus. In the preglomerular nucleus, a large number of neurons give rise to projections that terminate in the ipsilateral central posterior/prepacemaker nucleus. The reciprocal connection between the central posterior/prepacemaker nucleus and the preglomerular nucleus may be used to relay sensory information directly conveyed to one of the two nuclei indirectly to the other nucleus. The existence of at least some central posterior/prepacemaker nucleus neurons projecting to both the preglomerular nucleus and the pacemaker nucleus may provide the morphological basis for the transmission of an efference copy of electromotor information produced by neurons in the central posterior/prepacemaker nucleus to the preglomerular nucleus.

Substance P-like immunoreactivity in the brain of the gymnotiform fish Apteronotus leptorhynchus: Presence of sex differences

The distribution of substance P-like immunoreactivity (SPli) was charted in the brain of the gymnotiform fish Apteronotus leptorhynchus, and correlated with the circuitry underlying intraspecific electrocommunication. Cell bodies were found predominantly in the lateral hypothalamus and in certain paraventricular organs: nucleus preopticus periventricularis, anterior subdivision; anterior hypothalamus; nucleus posterioris periventricularis; nucleus recessus lateralis, medial subdivision 2; nucleus recessus posterioris and nucleus recessus lateralis, lateral subdivision. Cell bodies were also found in the rostral olfactory nucleus, ventral telencephalon (ventral and central subdivisions), the habenula, the vagal sensory and motor nuclei and in the subtrigeminal nucleus. The distribution of SPli fibers was similar in some respects to that reported for other vertebrates. SPli was found in the rhombencephalon associated with vagal afferent fibers and in the funicular nucleus (possibly related to nociception). In the diencephalon and midbrain SPli fibers were found in the habenular-interpeduncular tract, in the hypothalamus and pituitary. SPli fibers were also found in preoptic and forebrain areas. The most striking result was the sexually dimorphic SPli innervation of certain hypothalamic and septal nuclei, and of the prepacemaker nucleus (PPn), a diencephalic cell group which controls communication (‘chirping’) in gymnotiforms. The PPn and septal/hypothalamic nuclei were densely innevated by SPli in males but devoid of SPli in females.

Origins of the descending spinal projections in petromyzontid and myxinoid agnathans.

The origins of the descending spinal pathways in sea lampreys (Petromyzon marinus), silver lampreys (Ichthyomyzon unicuspis), and Pacific hagfish (Eptatretus stouti) were identified by the retrograde transport of horseradish peroxidase (HRP) placed in the rostral spinal cord. In lampreys, the majority of HRP-labeled cells were located along the length of the brainstem reticular formation in the inferior, middle, and superior reticular nuclei of the medulla, mesencephalic tegmentum, and nucleus of the medial longitudinal fasciculus. Labeled reticular cells included the Mauthner and Müller cells. Horseradish-peroxidase-filled cells were also present in the descending trigeminal tract, intermediate and posterior octavomotor nuclei, and a diencephalic cell group, the nucleus of the posterior tubercle. As in lampreys, the reticular formation of the Pacific hagfish was the largest source of descending afferents to the spinal cord. Labeled cells were found in the dorsolateral and ventromedial reticular nuclei, the dorsal tegmentum at the juncture of the medulla and midbrain, and the nucleus of the medial longitudinal fasciculus. Additional medullary cells projecting to the cord were located in the perivagal nucleus, the central gray, and the anterior and posterior magnocellular octavolateralis nuclei. The existence of reticulospinal and possible vestibulo-, trigemino-, and solitary spinal projections in lampreys and hagfishes and the wide distribution of these pathways in jawed vertebrates suggest that they evolved in the common ancestor of gnathostomes and both groups of jawless fishes. However, descending spinal pathways from the cerebellum, red nucleus, and telencephalon appear to be gnathostome characters.

Distribution of 5-hydroxytryptamine (serotonin) in the brain of the teleost Gasterosteus aculeatus L.

The distributions of serotoninergic neurons in the brain of the three-spined stickleback was demonstrated with the indirect peroxidase-antiperoxidase (PAP) immunohistochemical method with antibodies against serotonin. Serotoninergic perikarya were demonstrated in the brainstem reticular formation (nucleus raphe dorsalis, nucleus raphe medialis, and nucleus tegmenti dorsalis lateralis) and in the periventricular ventral thalamus and hypothalamus (nucleus ventromedialis thalami, nucleus posterioris periventricularis, nucleus recessus lateralis, and nucleus recessus posterioris). After pharmacological pretreatment of the animals with a monoamine oxidase inhibitor, serotoninergic perikarya were also visualized in area praetectalis and in the medial brainstem, caudal to nucleus raphe medialis. Whereas the cell groups of the brainstem give rise to both ascending and descending pathways, it was not possible to analyze the distribution of efferent projections from the diencephalic cell groups. Distribution of serotoninergic axons showed marked regional differences. Only scattered varicose fibers were demonstrated in the cerebellum, the facial lobes, and the lateral line lobes. In the mesencephalon, the dorsal periventricular tegmentum and the central gray receive only small numbers of serotoninergic axons, while torus semicircularis and the visual layers of tectum opticum are profusely innervated. In the diencephalon, the hypothalamus and ventral thalamus generally display the highest density of serotoninergic axons. Exceptions are found in nucleus glomerulosus and the ventromedial portion of lobus inferioris, where densities are low. In the telencephalon, the density of serotoninergic axons is very high in area dorsalis pars medialis and pars lateralis dorsalis, but low in area dorsalis pars dorsalis and pars lateralis ventralis, and intermediate in area ventralis.

Localization of neurons afferent to the optic tectum in longnose gars.

Afferent pathways to the optic tectum in the longnose gar were determined by unilateral tectal injections of HRP. Retrogradely labeled cells were observed in the ipsilateral caudal portion of the rostral entopeduncular nucleus and bilaterally in the rostral half of the lateral zone of area dorsalis of the telencephalon. The following diencephalic cell groups were also labeled following tectal injections: the ipsilateral anterior, ventrolateral, and ventromedial thalamic nuclei, the periventricular pretectal nucleus, and the central pretectal nucleus (bilaterally); the ventromedial thalamic and central pretectal nuclei revealed the largest number of labeled cells. At midbrain levels, retrogradely labeled cells were seen in the ipsilateral torus longitudinalis, nucleus isthmi, and accessory optic nucleus; cells were labeled bilaterally in the torus semicircularis and a rostral tegmental nucleus. Only a few cells were labeled in the contralateral optic tectum, suggesting that few of the fibers of the intertectal commissure are actually commissural to the tectum. At hindbrain levels, retrogradely labeled cells were seen bilaterally in the locus coeruleus, the superior, medial, and inferior reticular formations, the eurydendroid cells of the cerebellum, and the nucleus of the descending trigeminal tract; the contralateral dorsal funicular nucleus also exhibited labeling. Clearly, the tectum in gars receives a substantial number of nonvisual afferents from all major brain areas, most of which have been reported in other vertebrates. The functional significance of these afferent sources and their probable homologues in other vertebrate groups are discussed.

The localization of vasotocin and neurophysin neurons in the diencephalon of the pigeon, Columba livia.

Vasotocin (VT)- and neurophysin (NP)-synthesizing neurons were demonstrated by immunocytochemistry in the diencephalon of the pigeon, Columba livia. Three diencephalic regions contain VT-NP cells: (1) periventricular preoptic area and hypothalamus, including nucleus periventricularis magnocellularis (PVM); (2) lateral preoptic area and hypothalamus; and (3) dorsal diencephalon. The immunoreactive cells in each of these three regions were divided into groups based on cytology and topography. No differences were found in the location of VT and NP cell groups. The periventricular region contains three continuous cell groups (P1-P3) extending from the posteroventral preoptic area to the anterodorsal hypothalamus and PVM. The lateral region has two cell groups composed of medium- to large-sized cells associated with the quintofrontal tract (L1) or with the optic tract (L2), while a third group (L3) lies between these two cell groups. Two accessory cell groups reside in the dorsolateral hypothalamus; L4 contains scattered cells of varied size, whereas L5 has small- to medium-sized cells clumped together. The dorsal diencephalic cell groups are found in the following locations: (1) lateral and dorsal to the lateral forebrain bundle (DD1); (2) in the area ventral to the dorsomedial anterior thalamic nucleus and dorsolateral to PVM (DD2); and (3) at the dorsolateral border of nucleus rotundus (DD3). To avoid potentially inaccurate mammalian homologies, the cell group nomenclature denotes topographic position. Nevertheless, the presence of VT-NP cells in PVM and projections to the brainstem and spinal cord suggest a homology between PVM and some of the parvocellular subnuclei of the mammalian paraventricular nucleus.

Projections of the optic tectum in two teleost species.

The efferent pathways of the optic tectum have been investigated in the percomorph Eugerres and the berycomorph Holocentrus. A portion of the dorsal-dorsolateral region of the optic tectum was unilaterally resected by suction. The animals were perfused 6-30 days thereafter, and the brains were processed according to a modification (Method 7 in Ebbesson, '70) of the Fink-Heimer ('67) technique for the selective silver impregnation of degenerating axons and terminals. Three groups of fibers emerge from the lesioned region (a) a medial group, which runs towards the midline and terminates in the ipsilateral torus longitudinalis and the contralateral tectum; (b) an ascending group, which enters the dorsocaudal region of the diencephalon and terminates in pretectal cell groups, in the dorsomedial optic thalamic nucleus, and in the nucleus rotundus or prethalamicus; and (c) a descending group, which funnels down into the midbrain tegmentum. Here abundant terminals are given to dorsolateral cell groups and to the nucleus isthmi. A recurrent fascicle leaves the mainstream and ascends to terminate in scattered diencephalic cell groups, in the nucleus geniculatus posterior pars ventralis, and in the nucleus rotundus or prethalamicus. The bulk of descending fibers then forms an ipsilateral bundle, which gives terminals to the lateral reticular formation of mesencephalon and rhombencephalon, and a contralateral (i.e., the predorsal) bundle, which terminates in the medial reticular formation of mesencephalon and rhombencephalon.

Fluorescence histochemical and microspectrofluorometric mapping of dopamine and noradrenaline cell groups in the rat diencephalon

Summary In a combined fluorescence microscopical and microspectrofluorometric study, the distribution, morphology and amine content of diencephalic catecholamine-containing cell groups have been investigated in the rat brain. Topographically the diencephalic catecholamine cell bodies are confined to two fairly continuous cell systems well separated from each other: one mainly located in the periventricular region of the rostral and middle hypothalamus, and one in the dorsal and caudal hypothalamus and caudal thalamus. On the basis of morphology and amine content 4 main diencephalic cell groups have been distinguished: (1) the rostral periventricular dopamine cell group; (2) the dopamine cell group of the arcuate nucleus, constituting the dopaminergic tubero-hypophyseal neuronal systems; (3) the dorsal hypothalamic dopamine cell group; and (4) the caudal cell group, comprising both dopamine-containing and noradrenaline-containing cell bodies, extending from the periventricular gray matter of the caudal thalamus down into the posterior hypothalamic area. The prominent hypothalamic and thalamic dopamine and noradrenaline cell groups described in the present paper have hitherto been largely neglected. A more detailed neuro-anatomical knowledge of these neuronal systems should provide a basis for studies on their roles in diencephalic functions.