Dielectrophoretic Trapping Research Articles

Conventional Lateral Flow Immunoassay with Integrated Screen-Printed Electrodes for Dielectrophoretic Trapping of Bacteria Enabling Sensitive, Rapid Detection at Low Concentrations

Conventional Lateral Flow Immunoassay with Integrated Screen-Printed Electrodes for Dielectrophoretic Trapping of Bacteria Enabling Sensitive, Rapid Detection at Low Concentrations

Photovoltaic Charge Lithography on Passive Dielectric Substrates Using Fe:LiNbO3 Stamps

AbstractPhotovoltaic Fe:LiNbO3 is an outstanding material platform able to photo‐generate versatile charge patterns, useful for a broad variety of applications. However, in some cases, its photorefractive effect, light absorption, and active ferroelectric properties may interfere with the optimum operation of certain devices based on Fe:LiNbO3. Here, a novel optoelectronic method is proposed and demonstrated to transfer photovoltaic charge patterns from Fe:LiNbO3 to non‐photovoltaic passive substrates, thus removing these possible limitations. The method, denominated as photovoltaic charge lithography (PVCL), resembles the operation of a stamp and does not require external high‐voltage supplies or electron/ion beams. Upon contact between the active Fe:LiNbO3 stamp and a passive dielectric substrate, the light‐induced charge pattern can be faithfully mirrored on the passive substrate. The imprinted pattern is probed and characterized by dielectrophoretic and electrophoretic particle trapping. The results reveal that the charge builds up on the passive substrate during contact, allowing charge tunability. Moreover, arbitrary charge distributions can be flexibly tailored, using scanning laser beams or spatially structured light. Overall, PVCL opens the possibility of printing complex 1D/2D charge patterns of controlled polarity on different passive dielectric materials, enhancing the technological potential of Fe:LiNbO3 photovoltaic platforms.

Visual and Quantitative Analysis of the Trapping Volume in Dielectrophoresis of Nanoparticles.

Nanoparticle manipulation requires careful analysis of the forces at play. Unfortunately, traditional force measurement techniques based on the particle velocity do not provide sufficient resolution, while balancing approaches involving counteracting forces are often cumbersome. Here, we demonstrate that a nanoparticle dielectrophoretic response can be quantitatively studied by a straightforward visual delineation of the dielectrophoretic trapping volume. We reveal this volume by detecting the width of the region depleted of gold nanoparticles by the dielectrophoretic force. Comparison of the measured widths for various nanoparticle sizes with numerical simulations obtained by solving the particle-conservation equation shows excellent agreement, thus providing access to the particle physical properties, such as polarizability and size. These findings can be further extended to investigate various types of nano-objects, including bio- and molecular aggregates, and offer a robust characterization tool that can enhance the control of matter at the nanoscale.

Deformed graphene FET biosensor on textured glass coupled with dielectrophoretic trapping for ultrasensitive detection of GFAP

Numerous efforts have been undertaken to mitigate the Debye screening effect of FET biosensors for achieving higher sensitivity. There are few reports that show sub-femtomolar detection of biomolecules by FET mechanisms but they either suffer from significant background noise or lack robust control. In this aspect, deformed/crumpled graphene has been recently deployed by other researchers for various biomolecule detection like DNA, COVID-19 spike proteins and immunity markers like IL-6 at sub-femtomolar levels. However, the chemical vapor deposition (CVD) approach for graphene fabrication suffers from various surface contamination while the transfer process induces structural defects. In this paper, an alternative fabrication methodology has been proposed where glass substrate has been initially texturized by wet chemical etching through the sacrificial layer of synthesized silver nanoparticles, obtained by annealing of thin silver films leading to solid state dewetting. Graphene has been subsequently deposited by thermal reduction technique from graphene oxide solution. The resulting deformed graphene structure exhibits higher sensor response towards glial fibrillary acidic protein (GFAP) detection with respect to flat graphene owing to the combined effect of reduced Debye screening and higher surface area for receptor immobilization. Additionally, another interesting aspect of the reported work lies in the biomolecule capture by dielectrophoretic (DEP) transport on the crests of the convex surfaces of graphene in a coplanar gated topology structure which has resulted in 10 aM and 28 aM detection limits of GFAP in buffer and undiluted plasma respectively, within 15 min of application of analyte. The detection limit in buffer is almost four decades lower than that documented for GFAP using biosensors which is is expected to pave way for advancing graphene FET based sensors towards ultrasensitive point-of-care diagnosis of GFAP, a biomarker for traumatic brain injury.

Characterizing the Janus colloidal particles in AC electric field and a step towards label-free cargo manipulation

In recent years, researchers have been exploring Janus particles, a unique type of colloidal system, which has gained enormous attention across diverse fields, from soft matter physics to biology. These particles, possessing an asymmetric surface, can be manipulated in the fluid using external energy sources. This versatility makes them suitable for mimicking biological systems and applications like cargo transportation, drug delivery, biosensing, and environmental cleanup. The study focuses on the response of metal-dielectric (Ti-PMMA) Janus spheres in the AC field, created through the Physical Vapor Deposition (PVD) technique. When subjected to a uniform AC electric field, these particles exhibit different dynamic behaviors at various frequency ranges. The particles show diverse responses from induced-charge electrophoresis (ICEP: 500 Hz-80 kHz) to reversed motion (r: ICEP:100 kHz-1 MHz) and linear chain formation (1 MHz). Additionally, they randomly cluster near the lower characteristic frequency (800 Hz) of the ICEP, whereas they exhibit the 3D motion at frequencies (100 Hz) below this lower characteristic value. The mechanisms behind these dynamic phenomena could be explained by considering the concept of electric double layer (EDL), self-dielectrophoresis (sDEP), dipolar interactions, hydrodynamic interactions, and electrothermal effects. These active Janus Colloids are further leveraged to manipulate 1 µm diameter PMMA passive microparticles and E. coli bacteria at 1 kHz, showcasing their potential applications in cargo delivery under various microfluidic research areas. The manipulation of payloads could be explained based on dielectrophoretic trapping due to locally generated field gradients around the surface of the Janus sphere. Therefore, the present work offers an unprecedented opportunity to study such out-of-equilibrium complex active matter systems and create novel functional materials. Moreover, it could also be extended as a fuel-free microrobotics system for various biomedical applications such as sensing and on-demand drug or cargo delivery inside the microfluidics chamber.

High-Throughput Continuous Free-Flow Dielectrophoretic Trapping of Micron-Scale Particles and Cells in Paper Using Localized Nonuniform Pore-Scale-Generated Paper-Based Electric Field Gradients.

Dielectrophoresis (DEP) utilizes a spatially varying nonuniform electrical field to induce forces on suspended polarizable soft matter including particles and cells. Such nonuniformities are conventionally created using 2D or 3D micrometer-scale electrode arrays. Alternatively, insulator-based dielectrophoresis (iDEP) uses small micrometer-scale insulating structures to spatially distort and generate regions of localized field gradients to selectively trap, isolate, and concentrate bioparticles, including bacteria, viruses, red blood cells, and cancer cells from a suspending electrolyte solution. Despite significant advances in the microfabrication technology, the commercial adoption of DEP devices for soft matter manipulation remains elusive. One reason for low market penetration is a lack of low-cost and scalable fabrication methods to quickly microfabricate field-deforming structures to generate localized DEP-inducing electric field gradients. We propose here that paper-based devices can offer a low-cost and easy-to-use alternative to traditional iDEP devices. In this article, we demonstrate for the first time the ability to perform iDEP-style particle trapping using the naturally occurring micrometer-scale insulating porous structures of paper. In particular, we use polymeric laminated nonwoven fiberglass paper channels as a source of insulating structures for iDEP. We apply a flow of polarizable microparticles directly within the nonwoven channel and simultaneously drop an electric field perpendicular to the flow direction to induce DEP. We show the ability to readily trap and concentrate particles in paper by DEP with an applied voltage as low as 2 V using two different flow mechanisms: a constant fluid flow rate using an external pump and passive fluid flow by capillary wicking. Using a combination of micro computed tomography and finite element analysis, we then present a computational model to probe the microscale DEP force formation dynamics within the paper structure. This new paper-based iDEP platform enables the development of robust, low-cost, and portable next-generation iDEP systems for a wide variety of sample purification and liquid handling applications.

A Study on the Temperature Sensitivity of Nanosensors Based on Single-Walled Carbon Nanotubes in Ambient Conditions

Electron transport in nonisolated carbon nanotubes (CNTs) is highly influenced by their interactions with their environment. In this study, the effect of the local surroundings on the temperature sensitivity of single-walled CNTs is investigated at ambient temperature. For this study, CNTs are trapped in between two nanoelectrodes on a sapphire substrate using a dielectrophoretic trapping process. We find that it is possible to tune the temperature sensitivity by controlling the number of trapped nanotubes. The results indicate that the temperature sensitivity increases as the number of nanotubes decreases. For a device fabricated with a few CNTs, resistance changes at a rate of 3.24%/ <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$^{\circ}$</tex-math> </inline-formula> C, but the sensitivity of devices with many CNTs is significantly smaller with a resistance change of 0.19%/ <inline-formula xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink"> <tex-math notation="LaTeX">$^{\circ}$</tex-math> </inline-formula> C. We attribute this effect to changes in the interaction of the sapphire substrate with changes in the CNTs’ concentration.

Microfluidic device combining hydrodynamic and dielectrophoretic trapping for the controlled contact between single micro-sized objects and application to adhesion assays.

The understanding of cell-cell and cell-matrix interactions via receptor and ligand binding relies on our ability to study the very first events of their contact. Of particular interest is the interaction between a T cell receptor and its cognate peptide-major histocompatibility complex. Indeed, analyzing their binding kinetics and cellular avidity in large-scale low-cost and fast cell sorting would largely facilitate the access to cell-based cancer immunotherapies. We thus propose a microfluidic tool able to independently control two types of micro-sized objects, put them in contact for a defined time and probe their adhesion state. The device consists of hydrodynamic traps holding the first type of cell from below against the fluid flow, and a dielectrophoretic system to force the second type of object to remain in contact with the first one. First, the device is validated by performing an adhesion frequency assay between fibroblasts and fibronectin coated beads. Then, a study is conducted on the modification of the cellular environment to match the dielectrophoretic technology requirements without modifying the cell viability and interaction functionalities. Finally, we demonstrate the capability of the developed device to put cancer cells and a population of T cells in contact and show the discrimination between specific and non-specific interactions based on the pair lifetime. This proof-of-concept device lays the foundations for the development of next generation fast cell-cell interaction technologies.

Scalable Self-Limiting Dielectrophoretic Trapping for Site-Selective Assembly of Nanoparticles.

The absence of a versatile, scalable, and defect-free bottom-up assembly of nanoparticles with high precision has been a longstanding roadblock facing the large-scale integration of diverse nanoparticle-based devices. To circumvent this roadblock, we present a self-limiting dielectrophoretic approach to precisely align nanoparticles onto an array of electrodes over a large area, assisted by lithographically defined capacitors in series with the electrodes. We have experimentally verified that the on-chip capacitor can reduce the probability of trapping multiple particles at a given site, as the electric field is greatly weakened after the first nanoparticle bridges the electrodes. A 70% yield of single-nanowire assembly has been achieved, and key factors limiting the current yield are discussed. The yield is expected to further increase by improving the nanoparticle-electrode contact and reducing the capillary force during the drying process. We also demonstrate the versatility of this approach for scalable and site-selective alignment of various nanoparticles.

Dielectrophoretic Traps for Efficient Bead and Cell Trapping and Formation of Aggregates of Controlled Size and Composition.

We present a microfluidic dielectrophoretic-actuated system designed to trap chosen single-cell and form controlled cell aggregates. A novel method is proposed to characterize the efficiency of the dielectrophoretic trapping, considering the flow speed but also the heat generated by the traps as limiting criteria in cell-safe manipulation. Two original designs with different manufacturing processes are experimentally compared. The most efficient design is selected and the cell membrane integrity is monitored by fluorescence imaging to guarantee a safe-cell trapping. Design rules are suggested to adapt the traps to multiple-cells trapping and are experimentally validated as we formed aggregates of controlled size and composition with two different types of cells. We provide hereby a simple manufactured tool allowing the controlled manipulation of particles for the composition of multicellular assemblies.

(Digital Presentation)Room Temperature Sensor Using Dielectrophoretic Trapping of Carbon Nanotubes

This abstract presents the design and fabrication of a temperature sensor based on carbon nanotubes (CNTs). The sensor is fabricated by dielectrophoretic trapping of CNTs between two nanoelectrodes. The conductance of the sensor is highly sensitive to the temperature variation which makes it suitable for applications requiring accurate measurements. In addition, fast response and good stability and durability are the important advantages of the sensors made of CNTs.The structure of the device is shown in the figure. CNTs are trapped in between nanoelectrodes using the dielectrophoresis (DEP) technique. Dielectrophoresis is a phenomenon in which a force is applied to a dielectric particle when it is exposed to a non-uniform electric field [1]. Although dielectrophoresis provides an accurate method for trapping materials in a predefined area, this method suffers from a costly fabrication process when dealing with nanomaterials. In this study, we used a standard photolithography method to fabricate low-cost devices and an electronic circuit is used to apply the required electric field and monitor the gap for the presence of CNTs.The device fabrication process starts with the patterning and depositing gold nanoelectrodes and contacts using the photolithography method. The process is followed by converting the surface of the device except for the nanoelectrodes area and connection pads with an insulation layer of Silicon nitride. Since the electrodes are thin (50 nm) and CNTs are flexible, fabrication on the flexible substrates is also feasible.

Measurement of dielectric properties of cells at single-cell resolution using electrorotation.

Dielectric properties of a cell are biophysical properties of high interest for various applications. However, measuring these properties accurately is not easy, which can be exemplified by the large variations in reported dielectric properties of the same cell types. This paper presents a method for measuring the dielectric properties of cells at high frequency, especially lipid-producing microalgae, at single-cell resolution, by integrating an electrorotation-based dielectric property measurement method with a negative dielectrophoretic (nDEP) force-based single-cell trapping method into a single device. In this method, a four-electrode nDEP structure was used to trap a single cell in an elevated position in the center of another four-electrode structure that can apply electrorotational force. By measuring the speed of cell rotation under different applied electrorotation frequencies and fitting the results into a theoretical core-shell cell model, the dielectric properties of cells, including membrane capacitance and cytoplasm conductivity, could be obtained. This system was applied to measure the dielectric properties of lipid-accumulating microalga Chlamydomonas reinhardtii strain Sta6 by applying an electrorotation signal of up to 100MHz. By utilizing a broad frequency range and expanding the measurement spectra to a high frequency region, increased accuracy in fitting the dielectric parameters to a theoretical model was possible, especially the cytoplasm conductivity. The developed method can be used in various applications, such as screening microalgae based on their lipid production capabilities, separating cells of different dielectric properties, identifying different cell types, as well as conducting basic biophysical analyses of cellular properties.

Electrical Propulsion and Cargo Transport of Microbowl Shaped Janus Particles.

Herein the effective electrical propulsion, cargo trapping, and transport capabilities of microbowl-shaped Janus particles (JPs) are demonstrated and evaluated. These active JPs are made by deposition of Au and Ti layers onto sacrificial spherical polystyrene particles, followed by oxidation of the Ti to TiO2 . In contrast to the commonly studied spherical JP, the dual broken symmetry of both geometrical and electrical properties of the microbowl renders a strong dependence of its mobility and cargo loading on the order of the layering of Au and TiO2 . Specifically, an opposite direction of motion is obtained for interchanged layers of Au and TiO2 , using only electrical propulsion as the sole mechanism of motion. The concave side of the microbowl exhibits a negative dielectrophoretic trap of large size wherein trapped cargo is protected from hydrodynamic shearing, leading to an enhanced cargo loading capacity compared to that obtained using common spherical JP. Such enhanced cargo capability of the microbowl along with the ease of engineering it by interchanging the order of the layers are very attractive for future in vitro biological and biomedical applications.

Dielectrophoretic Trapping for Nanoparticles, High-Molecule-Weight DNA, and SYBR Gold Using Polyimide-Based Printed Circuit Board

Microelectrodes for electrokinetic detection have been employed to manipulate and collect nanomaterials and biomaterials. However, the process of microelectrode patterning has been researched in the semiconductor fabrication process, which is expensive and time-consuming. Here, we demonstrate dielectrophoretic trapping of 200 nm fluorescent polystyrene nanoparticles and λ-deoxyribonucleic acid with SYBR gold using dielectrophoretic trapping onto an interdigitated circular electrode (ICE). For dielectrophoretic trapping, an ICE is designed and an ordered flexible printed circuit board (FPCB), possessing a non-porous polyimide substrate, is constructed. The ICE FPCB could identify the dielectrophoretic trapping conditions and simulate them by the finite element method. The fluorescent molecules in the ICE FPCB are dielectrophoretically trapped at the finger edges of the ICE when a peak-to-peak voltage of 12 V is applied at 6 kHz for 15-20 min. Moreover, monitoring of the dielectrophoretic trapping effect of SYBR gold eliminated the false negative detection error.

Rapid Lipid Content Screening in Neochloris oleoabundans Utilizing Carbon-Based Dielectrophoresis.

In this study, we carried out a heterogeneous cytoplasmic lipid content screening of Neochloris oleoabundans microalgae by dielectrophoresis (DEP), using castellated glassy carbon microelectrodes in a PDMS microchannel. For this purpose, microalgae were cultured in nitrogen-replete (N+) and nitrogen-deplete (N−) suspensions to promote low and high cytoplasmic lipid production in cells, respectively. Experiments were carried out over a wide frequency window (100 kHz–30 MHz) at a fixed amplitude of 7 VPP. The results showed a statistically significant difference between the dielectrophoretic behavior of N+ and N− cells at low frequencies (100–800 kHz), whereas a weak response was observed for mid- and high frequencies (1–30 MHz). Additionally, a finite element analysis using a 3D model was conducted to determine the dielectrophoretic trapping zones across the electrode gaps. These results suggest that low-cost glassy carbon is a reliable material for microalgae classification—between low and high cytoplasmic lipid content—through DEP, providing a fast and straightforward mechanism.

Insulator-based dielectrophoretic focusing and trapping of particles in non-Newtonian fluids.

Insulator-based dielectrophoretic (iDEP) microdevices have been limited to work with Newtonian fluids. We report an experimental study of the fluid rheological effects on iDEP focusing and trapping of polystyrene particles in polyethylene oxide, xanthan gum, and polyacrylamide solutions through a constricted microchannel. Particle focusing and trapping in the mildly viscoelastic polyethylene oxide solution are slightly weaker than in the Newtonian buffer. They are, however, significantly improved in the strongly viscoelastic and shear thinning polyacrylamide solution. These observed particle focusing behaviors exhibit a similar trend with respect to electric field, consistent with a revised theoretical analysis for iDEP focusing in non-Newtonian fluids. No apparent focusing of particles is achieved in the xanthan gum solution, though the iDEP trapping can take place under a much larger electric field than the other fluids. This is attributed to the strong shear thinning-induced influences on both the electroosmotic flow and electrokinetic/dielectrophoretic motions.

Size-Selective Particle Trapping in Dielectrophoretic Corral Traps

The mechanism of a novel dielectrophoretic particle trapping system that selectively captures specific-size particles from a mixture of various-size particles is investigated. The results show that particles that fall within a certain range of vertical positions and velocities are captured, which enables size-selective trapping. The highest trapping efficiency is achieved at unexpectedly large microchannel heights, in contrast to conventional approaches that use shallow channels. This enables the rapid isolation and concentration of specific-size particles in a single step, which overcomes significant inefficiencies of current separation techniques caused by multiple treatments and dilution.

Perspectives on C-MEMS and C-NEMS biotech applications

Carbon microelectromechanical system (C-MEMS) and carbon nanoelectromechanical system (C-NEMS) have been identified as promising technologies for a range of biotech applications, including electrochemical biosensors, biofuel cells, neural probes, and dielectrophoretic cell trapping. Research teams around the world have devoted more and more time to this field. After almost two decades of efforts on developing C-MEMS and C-NEMS, a review of the relevant progress and addressing future research opportunities and critical issues is in order. This review first introduces C-MEMS and C-NEMS fabrication processes that fall into two categories: photolithography- and non-photolithography- based techniques. Next, a detailed discussion of the state of the art, and technical challenges and opportunities associated with C-MEMS and C-NEMS devices used in biotech applications are presented. These devices are discussed in the relevant sub-sections of biosensors, biofuel cells, intracorporeal neural probe, dielectrophoresis cell trapping, and cell culture. The review concludes with an exposition of future perspectives in C-MEMS and C-NEMS.

Combined quantum tunnelling and dielectrophoretic trapping for molecular analysis at ultra-low analyte concentrations

Quantum tunnelling offers a unique opportunity to study nanoscale objects with atomic resolution using electrical readout. However, practical implementation is impeded by the lack of simple, stable probes, that are required for successful operation. Existing platforms offer low throughput and operate in a limited range of analyte concentrations, as there is no active control to transport molecules to the sensor. We report on a standalone tunnelling probe based on double-barrelled capillary nanoelectrodes that do not require a conductive substrate to operate unlike other techniques, such as scanning tunnelling microscopy. These probes can be used to efficiently operate in solution environments and detect single molecules, including mononucleotides, oligonucleotides, and proteins. The probes are simple to fabricate, exhibit remarkable stability, and can be combined with dielectrophoretic trapping, enabling active analyte transport to the tunnelling sensor. The latter allows for up to 5-orders of magnitude increase in event detection rates and sub-femtomolar sensitivity.

Quantitative analysis of the three-dimensional trap stiffness of a dielectrophoretic corral trap.

Dielectrophoresis is a robust approach for the manipulation and separation of (bio)particles using microfluidic platforms. We developed a dielectrophoretic corral trap in a microfluidic device that utilizes negative dielectrophoresis to capture single spherical polystyrene particles. Circular-shaped micron-size traps were employed inside the device and the three-dimensional trap stiffness (restoring trapping force from equilibrium trapping location) was analyzed using 4.42μm particles and 1MHz of an alternating electric field from 6 VP-P to 10 VP-P . The trap stiffness increased exponentially in the x- and y-direction, and linearly in the z-direction. Image analysis of the trapped particle movements revealed that the trap stiffness is increased 608.4, 539.3, and 79.7% by increasing the voltage from 6 VP-P to 10 VP-P in the x-, y-, and z-direction, respectively. The trap stiffness calculated from a finite element simulation of the device confirmed the experimental results. This analysis provides important insights to predict the trapping location, strength of the trapping, and optimum geometry for single particle trapping and its applications such as single-molecule analysis and drug discovery.