This study, spanning 2016 to 2018, investigated diatom distribution in 324 Korean Peninsula estuaries, emphasizing the ecological impact of artificial dams. Examining environmental and biological variables, we focused on dam construction. OPEN estuaries exhibited higher urban/forest land cover and had more alkaline, saline, conductive, and oxygen-rich water. In CLOSE estuaries, agricultural farmland and nitrogen compound concentrations were higher. Dams in CLOSE estuaries correlated with lower pH, salinity, electrical conductivity, and dissolved oxygen. Regional variations were evident, with WEST OPEN estuaries showing higher salinity, turbidity, chemical oxygen demand, and total phosphorus. SOUTH estuaries displayed intermediate characteristics, while EAST OPEN estuaries had higher dissolved oxygen but lower temperature and turbidity.Diverse epilithic diatoms were identified, with OPEN estuaries displaying greater species richness, primarily dominated by Nitzschia inconspicua Grunow. CLOSE estuaries, conversely, exhibited higher richness with a different subdominant species. Indicator species analysis revealed taxa specific to estuaries and sea areas, with CLOSE ones exhibiting lower salinity and higher nitrogen/phosphorus concentrations. Nitzschia inconspicua dominated all estuaries, but subdominant species varied; N. fonticola (Grunow) Grunow in OPEN, N. paleaceae (Grunow) Grunow in CLOSE. CLOSE estuaries had higher species richness. In the West Sea, turbidity and TP levels affected diatom growth, with dams exacerbating turbidity and potentially disrupting the food chain.Results underscored intricate interactions between dams, land use, and diatom communities, offering foundational data for ecosystem management and biodiversity conservation in Korean Peninsula estuaries. Understanding the ecological consequences of artificial dams on diatom distribution is crucial for preserving estuarine health. Future research is recommended to explore additional factors influencing these ecosystems, particularly focusing on various organisms in West Sea estuaries for effective ecosystem management and conservation.