Nerodia Rhombifer Research Articles

Coinfection by Mycobacterium marinum and Mycolicibacterium fortuitum in a captive adult diamondback water snake causing disseminated mycobacteriosis with acute cutaneous ulceration.

An adult male captive diamondback water snake (Nerodia rhombifer) was found dead after a 1-d history of lethargy and cutaneous ulcers. The snake had eaten 2 sunfish (Mola spp.) 5 d before death. Gross examination revealed white-to-tan nodules in the lung and liver and segmental intestinal impactions with digested fish. Histopathology confirmed disseminated granulomas with numerous intrahistiocytic acid-fast bacteria in the skin, skeletal muscle, lung, liver, and intestines. Mycobacterium marinum and Mycolicibacterium fortuitum were identified by culture of the hepatic granuloma, followed by PCR and rpoB gene sequencing. To our knowledge, this is the first description of M. marinum and M. fortuitum coinfection in this species. Although M. fortuitum has been isolated from reptiles, lesions associated with its presence in tissues have not been described previously. Interestingly, the mineralization within granulomas that we observed in our case is not reported in mycobacterial infection in reptiles, whereas this finding is common in mammals.

First Record of the Diamond-backed Watersnake (Nerodia rhombifer) in Nebraska

First Record of the Diamond-backed Watersnake (Nerodia rhombifer) in Nebraska

Discovering high species diversity of Ophiotaenia tapeworms (Cestoda: Proteocephalidae) of watersnakes (Colubridae) in North America

Tapeworms of the genus Ophiotaenia La Rue, 1911 parasitize herptiles (= amphibians and ‘reptiles’) throughout the world, with about 100 species recognised as valid. In the present work, the North American species found in watersnakes (Colubridae) are reviewed. An examination of the holotype of Ophiotaenia perspicua La Rue, 1911, the type species of the genus, and other specimens from Nerodia rhombifer (Hallowell) revealed that two species were used for the species description. The ‘true’ O. perspicua has a small scolex and small, round suckers. This species is redescribed based on new material from Oklahoma, USA. The other species from N. rhombifer, Ophiotaenia laruei n. sp., has a larger scolex and larger, almost triangular suckers. Examination of the types of O. variabilis (Brooks, 1978) from N. rhombifer and N. cyclopion (Duméril, Bibron et Duméril) from Louisiana, USA has revealed that it is a mixture of two or more species. Because of poor quality of these specimens, it is not possible to adequately characterise O. variabilis, which is considered a species inquirenda. In addition, two new species are described from Nerodia fasciata confluens (Blanchard). Ophiotaenia currani n. sp. from Mississippi, USA is characterised by elongate, narrow proglottids, few testes, and a relatively long cirrus sac. Ophiotaenia tkachi n. sp. from Louisiana, USA is characterised by relatively short and wide proglottids, more testes, and an unusual terminal part of the vagina with folds. Morphologically similar tapeworms of N. fasciata confluens, N. erythrogaster (Förster), N. sipedon (Linnaeus), and Agkistrodon piscivorus (Lacépède) (Viperidae) from Arkansas and Oklahoma, USA, which are genetically nearly identical, are considered to be conspecific with O. tkachi n. sp. The present data suggest a high, previously undescribed species diversity of proteocephalid tapeworms in watersnakes in North America, and generally strict host specificity of these tapeworms.

Factors Influencing Activity and Detection of Species in a Cross Timbers Snake Assemblage

The Cross Timbers of North America is an ecotone between the eastern deciduous forests and central grasslands and exhibits a diverse composite snake fauna. Over a 3-yr period, we performed repeated nocturnal road surveys of snakes across three transects in Wise County, Texas, and evaluated species composition, relative abundances, vehicular mortality, detection probability, and the influence of environmental variables on snake activity. Sixteen taxa and 406 observations were recorded over 250 surveys, and the three most frequently encountered species had detection probabilities of approximately 0.20: Pantherophis obsoletus, Agkistrodon laticinctus, and Nerodia rhombifer. Only the two species of Agkistrodon present at our study area showed significant differences in count frequencies across the three transects. Covariate effects on overall snake activity were subtle, with barometric pressure, ordinal date, and ambient temperature better explaining variation in combined snake counts than humidity, wind speed, or lunar phase. Furthermore, peak snake activity corresponded closely to the timing of peak warm-season precipitation in the study area, suggesting that snake activity coincides with rainfall periodicity in the Cross Timbers region. However, results of site-occupancy models indicated that covariate effects were different across species, and that several common species did not show clear responses to any of the survey covariates.

Examining the shape and size of female and male genitalia in snakes using three-dimensional geometric morphometrics

Abstract Quantification of genital variation in males and females can inform our understanding of likely copulatory interactions and evolution of genital diversity. However, no studies have quantified genital shape variation within a single snake species or examined the shape and size of both the vaginal pouch and hemipenes. Here, we examine the shape and size of the genitalia of female and male diamondback water snakes, Nerodia rhombifer, using a three-dimensional automated landmark geometric morphometric approach on models of the lumen of the vaginal pouch and inflated hemipenes, applying these techniques for the first time to the genital shape of vertebrates. Vaginal pouch shape is significantly associated with body size and reproductive status. As females grow larger and become reproductive, the vaginal pouch enlarges, widens and becomes more bifurcated. In reproductive males, the shape of the hemipenes is also significantly associated with body size. As males grow larger, the hemipenes enlarge and widen; their bifurcation becomes more defined and the spines at the base become more prominent. Vaginal pouch and hemipenial centroid size are isometric with respect to body length. The centroid sizes of the hemipenes and vaginal pouch are not significantly different from one another, hence the genitalia match in size. Reproductive females and males covary in the degree of bifurcation and size of their genitalia. We demonstrate the utility of three-dimensional analysis in studies of the shape of soft tissues and advocate its use in future studies of genitalia.

Corticosterone in three species of free-ranging watersnakes: Testing for reproductive suppression and an association with body condition

The potentially suppressive effects of the hypothalamic-pituitary-adrenal (HPA) axis on the hypothalamic-pituitary-gonadal (HPG) axis revolve around the central role that glucocorticoids play in mobilizing energy. As an individual's energy balance becomes negative, the HPA axis helps mobilize energy and shifts energy expenditure away from reproduction toward maintenance and survival. While there is evidence in support of these relationships, substantial species variability exists. Studies in a greater diversity of species promise to enhance our understanding of the interactions between these axes. In this field study we tested for relationships among body condition, corticosterone, and sex steroid concentrations in three species of closely related watersnakes: the common watersnake (Nerodia sipedon), the diamondback watersnake (Nerodia rhombifer) and the queen snake (Regina septemvittata). Snakes were sampled before and after a 30 min acute confinement stressor and body condition was estimated using the scaled mass index. All three species exhibited robust elevations of corticosterone in response to acute stress, but only plasma progesterone was elevated by the acute stressor in queen snakes. There was no evidence for a suppression of sex steroid concentrations in any of the species. Body condition was negatively associated with baseline corticosterone in queen snakes and with post-stressor corticosterone in both queen and common watersnakes. Overall we found fairly strong support for the proposed link between corticosterone and energetics in two of the three watersnake species, but no support for the hypothesis that acute stressors are associated with reproductive suppression, at least as measured by steroid concentrations.

Plasma and tissue enzyme activities of banded water snakes (Nerodia fasciata) and diamondback water snakes (Nerodia rhombifer).

To measure plasma and tissue activities of alkaline phosphatase, alanine aminotransferase, aspartate aminotransferase (AST), creatine kinase, and γ-glutamyltransferase in 2 snake species. 6 banded water snakes (Nerodia fasciata) and 6 diamondback water snakes (Nerodia rhombifer). Blood was collected via the ventral tail vein to measure plasma enzyme activities. Animals were then euthanized, and samples of 9 tissues were collected from each snake: skeletal muscle, cardiac muscle, liver, spleen, lung, kidney, testicle, pancreas, and gallbladder. Tissues were frozen for 30 days, then homogenized and processed. Supernatants were collected and analyzed within 24 hours of processing. A linear mixed model was used to determine differences in enzyme activity between tissues and species and assess interactions between tissues and species. Activities of all enzymes were found to differ significantly among tissues. There were also significant differences between species for all enzyme activities, except AST activity. The kidney had the highest alanine aminotransferase and γ-glutamyltransferase activities. Alkaline phosphatase activity was significantly highest in liver and kidney tissues than in other tissue. Creatine kinase activity was highest in skeletal muscle, followed by cardiac muscle and kidney. AST activity was present in all tissues evaluated, but was highest in liver, kidney, and cardiac muscle in both species. Results reinforced the importance of characterizing the origin of tissue enzymes in reptiles to improve our understanding of biochemistry results and highlighted the differences that can exist in tissue enzyme activities between closely related species.

Facultative Parthenogenesis in a Diamondback Water Snake (<i>Nerodia rhombifer</i>)

Here we describe a case of facultative parthenogenesis in a diamondback water snake (Nerodia rhombifer). An adult female N. rhombifer kept in captivity produced four unfertilized ova, one stillborn and one live neonate in July 2016. The neonates had characteristic abnormalities in morphology and were determined as males, suggestive of a parthenogenetic event. Stillborn and live neonates along with the mother and four unrelated N. rhombifer were genetically screened at twelve microsatellite loci. Results confirmed that the reproductive event represented a case of facultative parthenogenesis. In light of previous reports of facultative parthenogenesis in the genera Nerodia and Thamnophis, these results suggest that this reproductive mode may be a widespread phenomenon in the Natricinae as a whole.

Photo Essay: Observations on the 2020 Occurrence and Mating Behavior of the Diamondback Water Snake (Nerodia rhombifer) at High Park, Derby, Kansas

Photo Essay: Observations on the 2020 Occurrence and Mating Behavior of the Diamondback Water Snake (Nerodia rhombifer) at High Park, Derby, Kansas

Role of phenotypic plasticity in morphological differentiation between watersnake populations.

An individual's morphology is shaped by the environmental pressures it experiences, and the resulting morphological response is the culmination of both genetic factors and environmental (non-genetic) conditions experienced early in its life (i.e. phenotypic plasticity). The role that phenotypic plasticity plays in shaping phenotypes is important, but evidence for its influence is often mixed. We exposed female neonate diamond-backed watersnakes (Nerodia rhombifer) from populations experiencing different prey-size regimes to different feeding treatments to test the influence of phenotypic plasticity in shaping trophic morphology. We found that snakes in a large-prey treatment from a population frequently encountering large prey exhibited a higher growth rate in body size than individuals in a small-prey treatment from the same population. This pattern was not observed in snakes from a population that regularly encounters small prey. We also found that regardless of treatment, snakes from the small-prey population were smaller at birth than snakes from the large-prey population and remained so throughout the study. These results suggest that the ability to plastically respond to environmental pressures may be population-specific. These results also indicate a genetic predisposition towards larger body sizes in a population where large prey items are more common.

No Sperm Morphometric Differences between Two Populations of Diamond-backed Watersnakes (Nerodia rhombifer) with Varying Resource Availability

Vast differences in available resources between habitats can have profound influences on aspects of an organism's life history, such as reproductive investment. Our study investigated how differences in nutrient availability affect sperm size in Diamond-backed Watersnakes (Nerodia rhombifer). We compared body size and sperm morphometrics between two populations with differing nutrient availability: a naturally occurring lake and a commercial fish farm. We hypothesized that prey availability affects sperm morphometrics. Our null hypothesis was that there would be no significant difference in sperm morphometrics between populations, whereas our alternative hypothesis was that the snakes from the fish farm would have significantly longer sperm. We measured total sperm length, sperm head length, sperm tail length, and snout–vent length (SVL). We then used two-tailed t-tests and AIC model selection to test our hypotheses. SVL was not correlated with sperm size. Furthermore, none of the sperm morphometrics were significantly different between the two populations. Finally, a nearly 1:1 correlation between sperm tail length and total sperm length was found. AIC model selection corroborated these results by choosing only sperm head length and sperm tail length as significant predictors of total sperm length. This is the first published study to explicitly compare sperm morphometrics between two populations of the same snake species. Additional studies of this nature are required to corroborate whether lack of significant differences in sperm morphometrics among snake populations are common.

Influence of prey size on reproduction among populations of Diamond-backed Watersnakes (Nerodia rhombifer)

Mean prey size often varies across landscapes, resulting in predator populations having differing access to energetic resources. Shifts in resource quality are likely to cause differences in energy allocation of reproduction. Thus, additional energy intake may lead to (i) increased offspring size, (ii) increased numbers of offspring, (iii) increased relative proportion of energy allocated to reproduction, (iv) increased absolute amount of energy allocated to reproduction or (v) increased energy allocated to growth of some other nonreproductive function, or (vi) some combination of the above outcomes. To test this hypothesis, we evaluated the reproductive allocation patterns of four populations of Diamond-backed Watersnakes (Nerodia rhombifer (Hallowell, 1852)) that differ in their mean prey size. Snakes at large prey sites produced longer, heavier babies compared with snakes from small prey sites. Statistical interactions among sites confounded our ability to compare differences in litter size, litter mass, and relative clutch mass. We suggest that increased prey size results in populations shifting reproductive allocation to increase offspring size. Effects of prey size on litter size, litter mass, and relative clutch mass remain unclear.

Patterns of morphological variation following colonization of a novel prey environment

AbstractColonization of new areas is accompanied by a variety of novel pressures, which can lead to rapid phenotypic change. We compared morphology of diamond‐backed watersnakes (Nerodia rhombifer) among populations of recently colonized fish farms to examine responses to a potential selective pressure, prey size and evaluated intersexual differences in phenotypic responses. Our data suggest not only have these populations experienced morphological change but also that males and females might be responding differently to the shared selective pressure. We found that male snakes from two sites raising primarily small fish had smaller cranial elements than males from one of the sites raising large fish and did not differ from the other large‐fish site. Similar to their male conspecifics, we found that females from both large‐prey sites had longer quadrates than one of the small‐prey sites. In addition, females from large‐fish populations reached greater snout‐vent lengths than females from small‐fish sites. These findings are consistent with an adaptive response to prey size, and also a differential response between the sexes. Our study demonstrates the potential for rapid phenotypic response to a strong selective pressure following colonization.

Preliminary Examination of Cultured Fish Consumption by the Diamondback Water Snake Nerodia rhombifera

The black hole so called for the lack of accounting, in aquaculture may account for unexplained losses of 20% or more per year in cultured organisms. Among potential causes are poor water quality and disease episodes, predation by birds, mammals, and reptiles/amphibians, and theft. At the Aquaculture Research Station of the University of Arkansas at Pine Bluff the diamondback water snake was commonly encountered. These live-bearing snakes of up to 2 m in length were observed consuming cultured channel catfish and baitfish, including goldfish. To begin to assess their capacity for damage, we held 3, 0.6-0.9 m adult snakes in large tanks. Each tank was supplied with a small water source for the goldfish, golden shiners and catfish fingerlings added, ad libitum. The consumption was monitored for three weeks. Consumption was no fish, 5 fish, and 1 fish; for an average of 0.7 fish/individual/week. Based on daytime observations of 5-10 snakes per 0.1 ha pond (likely an underestimate due to nocturnal activity in warm weather and under harassment) at the station and these results, an estimated loss of 5-10% per year may be expected.

Physical and Physiological Costs of Reproduction in Watersnakes

Estimating the cost of reproduction is pivotal to understanding the trade-off between current and future reproductive success, a key prediction in life-history theory. Increases in the cost of each reproductive attempt theoretically reduce future reproductive ability. Further, costs may change as individuals grow thus changing the nature of this trade-off. Measuring changes in female locomotor ability during reproduction has been one effective method to measure the cost of reproduction for females. We measured female Diamond-backed Watersnake (Nerodia rhombifer) swimming speed during and after pregnancy to determine if there was a loss of locomotor ability. We then correlated these speeds with measures of reproductive burdening (as estimated by relative clutch mass) and body size to investigate if increased reproductive investment and body size changed locomotor ability and subsequent cost of reproduction. Female snakes swam slower during pregnancy than after. Larger relative clutch masses resulted in slo...

Differential growth of body segments explains ontogenetic shifts in organ position for the Diamondback Water Snake (Nerodia rhombifer)

As snakes grow, their organs move anteriorly relative to body size. We explored a developmental explanation for the ontogenetic shift in the relative position of internal organs for snakes using the Diamondback Water Snake (Nerodia rhombifer (Hallowell, 1852)). With age, this water snake’s heart, liver, small intestine, and right kidney move anteriorly by 2.5–5.0 percentage points of snout–vent length. The number of precaudal vertebrae did not vary due to size or sex. The anterior edge of the heart, liver, small intestine, and right kidney were typically aligned within a span of 4–8 vertebrae that likewise did not differ as a function of size or sex. Snakes exhibited a positive relationship between the number of precaudal vertebrae and the vertebra number aligned with each organ. Total length, centrum length, centrum width, ball width, height, and mass of eight vertebrae sampled at consistent vertebral number revealed that vertebrae in the middle region of the body grow at a greater rate than vertebrae at the anterior or distal ends of the body. For N. rhombifer, the observed forward shift in relative organ positions is the product of regional differences in the growth of body segments. Predictably, these differences arise from a developmental program generated by the differential expression of Hox genes.

The relationship of head morphology and diet among three sympatric watersnake species

Abstract Investigating dietary resource utilization and head morphology of gape-limited predators can provide a basis for understanding interspecific competition and species coexistence. For sympatric species, convergence of head morphology can indicate similar prey or foraging strategies while divergence can suggest competition, resource partitioning or expansion into new habitats. Sexual dimorphism can further complicate the head morphology-diet relationship by potentially reducing intraspecific competition. To understand the relationship between head morphology and diet, we studied three sympatric species, the plain-bellied (Nerodia erythrogaster), diamondback (N. rhombifer), and northern (N. sipedon) watersnakes in western Kentucky. All three species inhabit similar wetlands and feed on amphibians and fishes. The anurophagous plain-bellied watersnakes had longer, narrower heads that likely allow snakes to capture anuran metamorphs and froglets while facilitating movement through dense vegetation. The piscivorous diamondback watersnake had a wider head, which would enhance contact with prey in this open-mouth forager, and smaller interocular distance, resulting in dorsally placed eyes that facilitate seeing fish prey from below. The mostly piscivorous northern watersnake had an intermediate, generalized head shape, which may reflect the typically wide diet range of this species. Head shape did not differ between snake sexes but diamondback and northern females had larger heads than males and fed more frequently on fishes. The relationship between head morphology and diet highlights potential interspecific and intersexual competition and aids in the understanding of species coexistence patterns.

Mutations to the cardiotonic steroid binding site of Na+/K+-ATPase are associated with high level of resistance to gamabufotalin in a natricine snake

Although toads are defended by bufadienolide toxins, some snakes have evolved resistance to bufadienolides and feed heavily on toads. We compared resistance in Nerodia rhombifer, which possesses mutations that confer target-site resistance, to Pituophis catenifer, which lacks those mutations. Even at the highest dosage tested, Nerodia showed no effects, whereas the lowest dose was lethal to Pituophis. Our results demonstrate a striking level of resistance to bufadienolides in a species possessing the mutations for resistance.

Ontogenetic shifts and spatial associations in organ positions for snakes

Snakes possess an elongated body form and serial placement of organs which provides the opportunity to explore historic and adaptive mechanisms of organ position. We examined the influence of body size and sex on the position of, and spatial associations between, the heart, liver, small intestine, and right kidney for ten phylogenetically diverse species of snakes that vary in body shape and habitat. Snake snout–vent length explained much of the variation in the position of these four organs. For all ten species, the position of the heart and liver relative to snout–vent length decreased as a function of size. As body size increased from neonate to adult, these two organs shifted anteriorly an average of 4.7% and 5.7% of snout–vent length, respectively. Similarly, the small intestine and right kidney shifted anteriorly with an increase in snout–vent length for seven and five of the species, respectively. The absolute and relative positioning of these organs did not differ between male and female Burmese pythons (Python molurus). However, for diamondback water snakes (Nerodia rhombifer), the liver and small intestine were more anteriorly positioned in females as compared to males, whereas the right kidney was positioned more anteriorly for males. Correlations of residuals of organ position (deviation from predicted position) demonstrated significant spatial associations between organs for nine of the ten species. For seven species, individuals with hearts more anterior (or posterior) than predicted also tended to possess livers that were similarly anteriorly (or posteriorly) placed. Positive associations between liver and small intestine positions and between small intestine and right kidney positions were observed for six species, while spatial associations between the heart and small intestine, heart and right kidney, and liver and right kidney were observed in three or four species. This study demonstrates that size, sex, and spatial associations may have potential interacting effects when testing evolutionary scenarios for the position of snake organs.

A test of energetic trade-offs between growth and immune function in watersnakes.

Energy budgets explain how organisms allocate energetic intake to accomplish essential processes. A likely life history trade-off occurs between growth and immune response in juvenile organisms, where growth is important to avoid predation or obtain larger prey and immune response is essential to survival in the presence of environmental pathogens. We examined the innate (wound healing) and adaptive (lymphoid tissue, thymus and spleen) components of immune response along with growth in two populations of the diamond-backed watersnake Nerodia rhombifer raised in a common environment. We found that neonate snakes born to females from populations characterized by different predator and prey environments did not differ in energetic intake, but snakes from the population containing large prey grew significantly faster than those from a population containing small prey. Thymus mass, when corrected for body mass, was larger in snakes from the small prey population than in snakes from the large prey population. Additionally, the snakes from the population containing small prey healed significantly faster than those from the population containing large prey. Thus, we detected a negative correlation between growth (over a 4-month period) and wound healing across populations that is suggestive of an energetic trade-off between growth and immune response. The differences observed in growth and immune response among these two populations appear to suggest different energy allocation strategies to maximize fitness in response to differing conditions experienced by snakes in the two populations.