An examination of a section of the horn sheath of Japanese serow (Capricornis crispus) showed that the number of growth layers in the keratinized zone correlated with age. Patterns of cementum annulation were also valid criteria for accurately estimating age. These methods were confirmed by using 18 known-age samples. The reliability of horn ring counts was checked by annulations in both horns and teeth and were found to be a useful means of estimating age. J. WILDL. MANAGE. 49(1):152-156 Examination of age structure is important in understanding the population dynamics of any species and, thus, a reliable technique for determining age is needed. Current studies of bovids use two major methods for age determination. The first, sectioning of mandibular teeth to count cementum annuli, has become an accepted technique (Armstrong 1965, Novakowski 1965, Hemming 1969, Pekelharing 1970, Turner 1977). This method undeniably yields useful results but involves considerable time and effort. A second technique is a horn segment count, which has been traditionally used by game biologists (Murie 1944, Welles and Welles 1961, Woodgerd 1964, Caughley 1965, Knaus and Schrbder 1983, Koubek and Hrabe 1983). The validity of this method has been established from a tagging-aging study for bighorns (Geist 1966) and by comparisons with horn segment and dental annulation (Turner 1977). Whenever this method is adopted, a problem may occur in distinguishing between true age rings and occasional obscure rings. The purpose of this study was to develop a simple and accurate technique for determining age in Japanese serow based on the layered structure of the horn sheath and teeth. In addition, the reliability of the horn segment count was checked by internal examinations of the horn sheaths. I am indebted to the Dep. of Vet. Anat., Gifu Univ., headed by M. Sugimura, for assistance in collecting samples. I wish to thank N. Maruyama, Tokyo Univ. of Agric. and Technol., and K. Yasui, Kyoto Munic. Zoo, for their invaluable assistance and encouragement. K. Tokita, R. Higashi, and H. Higashi, Res. Cent. for Jpn. Wildl., and M. Kanamori, Aichi Univ. of Educ., provided much valuable help throughout the study. I would also like to thank Y. Ono, Kyushu Univ., and M. Asahi, Hyogo Coll. of Med., for many helpful suggestions and encouragement. I gratefully acknowledge R. Kishimoto, Osaka City Univ., for supplying known-age samples. This work was supported in part by a Grant-in-Aid for Sci. Res. from the Minist. of Educ., Sci. and Cult., Japan. MATERIALS AND METHODS The samples for this study included teeth and horns from about 1,000 Japanese serows collected from three sources from 1977 to 1982. The first 936 samples were collected from a special population control experiment in Gifu a d Nagano Prefectures in the central part of Honshyu. They were collected after indiscriminate shooting of individuals from December to Ma ch. The second sample group, 24 serows of both sexes that died natural deaths, were collected from a wide range of localities in Honshyu and Shikoku. Finally, the teeth and horns of 18 known-age serows (from ? to 71/2 years old) were used to verify the reliability of the technique. Two of these were wild and 16 were pen-reared serows. The maximum length and maximum diameter of the horns were measured to the nearest millimeter using vernier calipers, and the horns were weighed to the nearest milligram on a dial scale. A median sagittal section was cut from the horn sheath with a circular saw. The section was ground to a thickness of 2 mm on a rotary surface grinder with a graded series of carborundum powders and cooled with water. It was then decolored in a 5% hydrogen peroxide solution for 24 hours or longer, and rinsed with tap water for 2 hours. The polished face of the horn section was etched by immersing it in a solution of 10% sodium hydroxide for about ?1/ hour. The section was rinsed with tap water and