Diagnostic Sections Research Articles

Analytical and clinical validation of direct detection of antimicrobial resistance markers by plasma microbial cell-free DNA sequencing.

This manuscript is ideally suited for the Innovative Diagnostic Methods sections as it reports the analytical and clinical validation of a novel application of plasma microbial cell-free DNA sequencing for direct detection of seven selected antimicrobial resistance markers in 18 target pathogens. Clearly, it has potential clinical utility in optimizing therapy and was incorporated into the Karius test workflow in September 2023. In addition, the workflow could readily be adapted to expand the number of target bacteria and antimicrobial resistance markers as needed.

Using positional tracking to improve abdominal ultrasound machine learning classification

Diagnostic abdominal ultrasound screening and monitoring protocols are based around gathering a set of standard cross sectional images that ensure the coverage of relevant anatomical structures during the collection procedure. This allows clinicians to make diagnostic decisions with the best picture available from that modality. Currently, there is very little assistance provided to sonographers to ensure adherence to collection protocols, with previous studies suggesting that traditional image only machine learning classification can provide only limited assistance in supporting this task, for example it can be difficult to differentiate between multiple liver cross sections or those of the left and right kidney from image post collection. In this proof of concept, positional tracking information was added to the image input of a neural network to provide the additional context required to recognize six otherwise difficult to identify edge cases. In this paper optical and sensor based infrared tracking (IR) was used to track the position of an ultrasound probe during the collection of clinical cross sections on an abdominal phantom. Convolutional neural networks were then trained using both image-only and image with positional data, the classification accuracy results were then compared. The addition of positional information significantly improved average classification results from ∼90% for image-only to 95% for optical IR position tracking and 93% for Sensor-based IR in common abdominal cross sections. While there is further work to be done, the addition of low-cost positional tracking to machine learning ultrasound classification will allow for significantly increased accuracy for identifying important diagnostic cross sections, with the potential to not only provide validation of adherence to protocol but also could provide navigation prompts to assist in user training and in ensuring adherence in capturing cross sections in future.

Использование диагностического инструмента «Матрица коммуникации» в работе с ребенком с тяжелой осложненной формой аутизма

<p style="text-align: justify;"><strong>Objectives. </strong>At the moment, there are extremely insufficient diagnostic tools suitable for examining the communicative sphere of children with severe complicated forms of autism, and recorded practical experience of their use in organizing psychological and pedagogical support for such children. The purpose of the study is to test the diagnostic tool “Communication Matrix” in working with a child of primary school age with a severe complicated form of autism (autism spectrum disorder (ASD), moderate intellectual disability, systemic speech under-development, visual impairment, atopic dermatitis), who was learning the skills of communication using the PECS image exchange communication system.</p> <p style="text-align: justify;"><strong>Methods. </strong>Diagnostic tool “Communication Matrix”. Data collection was carried out through observation of the child in free and educational activities for one year, and conversation with parents. The study is presented by three diagnostic sections and their analysis.</p> <p style="text-align: justify;"><strong>Results</strong>. At the stage of primary diagnosis, the child’s communicative characteristics were described and comments were given regarding the possibilities of taking them into account using the technique. Formed, partially formed and unformed communication skills of a child at the level of unintentional and intentional behavior, non-standard and standard communication, concrete and abstract symbols and language are identified. The objectives of correctional and developmental work are formulated based on a list of communication skills that are in the process of formation. The tasks were included in a special individual child development program and were implemented during the school day by a team of specialists, then reinforced at home with the participation of a private teacher and family members. At the final diagnostic stage, positive dynamics were noted in the development of communication at all levels. After a break (summer holidays), a regression of some skills was noted at the level of unintentional and intentional behavior, standard communication and specific symbols. </p> <p style="text-align: justify;"><strong>Conclusion. </strong>The “Communication Matrix” tool made it possible to conduct an initial diagnosis of the communication sphere of a child with a severe complicated form of autism and determine the tasks of correctional and developmental work. With the help of the instrument, changes in the child’s communicative sphere were recorded during continuous correctional and developmental education and during a break from it. Also, during the work, the advantages and limitations of the technique in relation to a specific diagnostic case were highlighted.</p>

Fluorescence confocal microscopy-complete digitization of pathology

Fluorescence-based confocal microscopy (FCM) can be used to create virtual H&E sections in real time. So far, FCM has been used in dermato-, uro-, and gynecopathology. FCM allows the creation of acompletely digitized frozen section, which could potentially replace conventional frozen sections in the future. The aim of the current work is to implement FCM technology as acomponent of fully digitized processes in the pathological workflow. For this purpose, the current use of FCM in liver transplant pathology will be extended to other disciplines such as urology and otorhinolaryngology. The FCM technique continues to be used prospectively on native tissue samples from potential donor livers. Conventional frozen sections are used comparatively to virtual FCM scans. The data show anearly perfect agreement for the detection of cholangitis, fibrosis, and malignancy, and ahigh level of agreement for, e.g., macrovesicular steatosis, inflammation, steatohepatitis, and necrosis between virtual FCM scans and conventional routine diagnostic frozen sections. Since the availability of time- and cost-intensive frozen section diagnostics in the context of transplant pathology in continuous operation (24/7) is now only established at very few university centers in Germany due to an increasing shortage of specialists, the use of FCM could be an important building block in the current process leading towards afully digitized pathology workflow and should thus be extended to various disciplines.

Electrodiagnostic reporting preferences of referring physicians: An exploratory survey.

Electrodiagnostic (EDX) studies play a crucial role in the evaluation of patients with peripheral nervous system disorders. Accurate and succinct communication of test results is critical to patient safety and clinical decision-making. The objective of this study was to explore EDX reporting preferences of referring physicians to improve quality of communication and patient care. An online survey was developed, and a purposive sampling strategy was used to recruit physicians in the authors' professional networks. Quantitative and qualitative survey data underwent frequency and thematic analyses, respectively. There were 40 respondents, including: 21 non-surgical specialists, 12 surgical specialists, and 7 family physicians. Sections rated as most critical were diagnostic impression (97%) and summary/interpretation (72%). Only 18% reported numeric data as critical to their needs, preferring this data to be formatted as bullet points or tables without nerve conduction study waveforms. Regarding the format of the data summary and diagnostic impression sections, the majority of respondents preferred bullet points rather than paragraphs. The results of this exploratory survey suggest that physicians who refer patients for EDX studies prefer reports that emphasize the interpretation of EDX data and a clear diagnostic impression, particularly in bullet point format. This project highlights important preferences and how they compare to recommended reporting guidelines, which may help improve communication and ultimately patient care. Future efforts should explore larger sample sizes with all key stakeholders in the EDX process to better understand reporting styles and preferences with greater nuance and context.

Application of natural language processing to post-structuring of rectal cancer MRI reports

To evaluate a natural language processing (NLP) system for extracting structured information from the free-form text of rectal cancer magnetic resonance imaging (MRI) reports written in Chinese. A rule-based NLP model that could extract 11 key image features of rectal cancer was constructed using 358 MRI reports of rectal cancer written between 2015 and 2021. Fifty reports written before 2015 and 50 written after 2021 were used as test datasets, and the reference standard was determined by manual extraction of information by two radiologists. The length and reporting rate of image features in pre-2015 and post-2021 datasets, as well as the accuracy, precision, recall, and F1 score of feature extraction by the NLP system, were compared. The time required for the NLP to extract data was compared with that required by the radiologists. Reports written after 2021 had longer diagnostic impression sections than reports written before 2015. The reporting rate of key imaging features of rectal cancer was 36.55% before 2015 and 79.82% after 2021. The accuracy, precision, recall, and F1 score of NLP for correct extraction of values from reports were 93.82%, 95.63%, 87.06%, and 91.15%, respectively, for pre-2015 reports, and 92.55%, 98.53%, 94.15%, and 96.29%, respectively, for post-2021 reports. NLP generated all the structured information in <1 second. The NLP system with rule-based pattern matching achieved rapid and accurate structured processing of rectal cancer MRI reports. MRI reports with structured templates are more suitable for NLP-based extraction of information.

Multi-candidate immunohistochemical markers to assess radiation response and prognosis in prostate cancer: results from the CHHiP trial of radiotherapy fractionation.

Protein markers of cellular proliferation, hypoxia, apoptosis, cell cycle checkpoints, growth factor signalling and inflammation in localised prostate tumours have previously shown prognostic ability. A translational substudy within the CHHiP trial of radiotherapy fractionation evaluated whether these could improve prediction of prognosis and assist treatment stratification following either conventional or hypofractionated radiotherapy. Using case:control methodology, patients with biochemical or clinical failure after radiotherapy (BCR) were matched to patients without recurrence according to established prognostic factors (Gleason score, presenting PSA, tumour-stage) and fractionation schedule. Immunohistochemical (IHC) staining of diagnostic biopsy sections was performed and scored for HIF1α, Bcl-2, Ki67, Geminin, p16, p53, p-chk1 and PTEN. Univariable and multivariable conditional logistic regression models, adjusted for matching strata and age, estimated the prognostic value of each IHC biomarker, including interaction terms to determine BCR prediction according to fractionation. IHC results were available for up to 336 tumours. PTEN, Geminin, mean Ki67 and max Ki67 were prognostic after adjusting for multiple comparisons and were fitted in a multivariable model (n=212, 106 matched pairs). Here, PTEN and Geminin showed significant prediction of prognosis. No marker predicted BCR according to fractionation. Geminin or Ki67, and PTEN, predicted response to radiotherapy independently of established prognostic factors. These results provide essential independent external validation of previous findings and confirm a role for these markers in treatment stratification. Cancer Research UK (BIDD) grant (A12518), Cancer Research UK (C8262/A7253), Department of Health, Prostate Cancer UK, Movember Foundation, NIHR Biomedical Research Centre at Royal Marsden/ICR.

Снижение психологических барьеров принятия ответственности у гандболистов-подростков: лонгитюдное исследование

The paper reflects the results of a formative experiment aimed at increasing the readiness of adolescent athletes to take responsibility by reducing the psychological barriers that make it difficult. Responsibility as a personal quality is relevant in any activity, and adolescence is a sensitive period of its manifestation. This demonstrates the resource component of adolescence for the transition to adulthood and accompanies success in determining one’s place in activity and socialization. The development of responsibility occurs due to its formation from childhood, in the process of learning by example and the transfer of freedom of action in feasible matters, which often contradicts our habitual paternalistic position of upbringing and involuntary support of infantilism of youth. The study of the literature and the earlier ascertaining stage of the study made it possible to identify personality qualities that act as barriers to the manifestation of responsibility in adolescence; they are: anxiety, low level of motivation and self-management, negative coping strategies (escaping or distancing), blaming other people or circumstances. This made it possible to select diagnostic material for evaluating the results of the formative experiment, based on the following six methods: determining the level of responsibility, motivation, self-regulation, coping strategies, anxiety, the level of group development, assessing the relationship between the coach and athletes. The longitudinal study involved 16 adolescent athletes from Krasnodar. The formative experiment of responsibility development was carried out using the methodology of psychological training in a youth handball team for a year. The effectiveness of the training was evaluated by three diagnostic sections. Changes in the absolute values of the measured indicators and correlations between them were taken into account. The results of the study showed that the factor preventing the removal of psychological barriers to accept responsibility is excessive anxiety of young athletes. The study demonstrates the need and importance for longitudinal psychological work with athletes.

Использование поляризационно-интерференционной микроскопии для определения оптических свойств фетальных эритроцитов в тканях

Objective: to evaluate the possibility of using polarization interference microscopy for morphological diagnostics of fetal erythrocytes in tissues. Materials and methods. Blood smears used during the diagnostic and treatment process and histological sections prepared from autopsy material were used. During the diagnosis, a polarization-interference microscope PZO BIOLAR PI with a microphotometric nozzle UPI was used to determine the optical density and the measure of light transmission in erythrocytes. The method of polarization-interference microscopy contributed to the study of optical properties in individual erythrocytes. The optical properties of erythrocytes containing fetal-type hemoglobin and erythrocytes with adult-type hemoglobin were compared. The difference in the composition of the structure of erythrocytes determined the difference in light transmission and refraction. Based on the results of light transmission, the optical density of fetal and adult erythrocytes was calculated. In the conducted study of clinical blood smears, in neonatal patients, 60 measurements of the light transmission coefficient and 60 similar studies were performed in patients of the comparison group - persons older than 18 years. In the study of autopsy material, 120 measurements of the erythrocyte light transmission coefficient were carried out in placental tissue samples (chorionic villi and interstitial space). Results. The optical properties of erythrocytes were determined both in blood smears and in placental tissue preparations. Erythrocytes in blood smears with HbF had an increased light transmission coefficient in relation to the optical properties of erythrocytes with HbA in the control group. During the study of placental tissues, it was revealed that the indicators of the optical density of maternal erythrocytes with HbA in the erythrocytes of the interstitial space differed in higher values of indicators compared with the optical density of fetal erythrocytes with HbF in the vessels of chorionic villi. Conclusion. The determination of differences in the physico-optical properties of erythrocytes with fetal and adult hemoglobins makes it possible to determine the affiliation of erythrocytes both in blood smears and in the interstitial space of the placenta.

Sonographic Features in the Placenta Accreta Spectrum

Background: Abnormally invasive placenta is defined as abnormal adhesion to the implantation site, it is a spectrum of abnormal placentation that leads to obstetric hemorrhage, knowledge of risk factors and ultrasound evaluation is what allows antenatal diagnosis allowing transfer to specialized centers, surgical planning, and modifying the maternal outcome, causing a decrease in maternal morbidity and mortality. Objective: To describe the main ultrasound signs found in patients diagnosed with abnormally invasive placenta. Methodology: Cross-sectional, descriptive, retrospective study, carried out through a bibliographic review in the databases of PubMed, Scholar.google.com, SciELO, MEDLINE, of articles published in English and Spanish between 1992 and 2020. The following methods were used. Keywords Mesh (Medical Subject Headings): placenta accreta; previous placenta; prenatal diagnosis; abnormally invasive placenta; placenta percreta; abnormally attached placenta. Inclusion criteria: case report-type articles that include pregnant patients with placenta accreta, associated risk factors and diagnostic evaluation sections, as well as a systematic review and meta-analysis on the prenatal diagnosis of placenta accreta, whose results were analyzed by analysis of frequency. Results: From the reviewed studies, it was observed that 66.7% of the patients had cesarean section as the predominant uterine surgery, ultrasound findings such as the loss of the hypoechoic zone in 35.7% of the cases; the presence of placental lacunae in 28.6%; myometrial thinning in 16.7%; as well as disruption of the uterus-bladder interface in 7.1%, in 54.8% intraoperative diagnosis was made. Conclusions: Placental accreta is an increasingly frequent pathology, the incessant search for alternatives to reduce maternal death, the ultrasound characterization of the most frequent signs help timely prenatal detection

Formation of the professional culture of future teachers-speech therapists through the use of innovative technologies

Еducation is aimed at individualization, improvement of the professional route and the process of professional training of future speech therapists. Of particular importance are issues of professional, personal and moral culture, which embody the positive socio-personal achievements of pedagogical skills, personal values and communicative interaction in the system of special and inclusive education to be followed by both children and their parents.&#x0D; The purpose of the work is to determine the criteria, indicators and diagnostic tasks of formation of the professional culture of future speech therapists, to diagnose the state of formation of the professional culture of future speech therapists.&#x0D; Diagnostic sections allowed us to determine the criterion-indicator base of formation of the professional culture of future teachers-speech therapists.&#x0D; The culture of professional communication reflects the level of proficiency in reference speech in compliance with all established requirements of the Ukrainian language (orthoepic norms, lexical and grammatical design, etc.), intonation and content saturation of expression, degree of erudition and general tolerance, conscious and respectful attitude to the subject process.&#x0D; Personal and professional competence reflects the degree of formation of value professional orientations of the individual, dynamic activity, non-standard and flexible thinking, constant enrichment of special knowledge, interests, skills, broadening horizons, creative understanding of non-standard pedagogical problem situations.&#x0D; We have developed a set of diagnostic tasks for each of the selected criteria and formed a scale for evaluating their implementation.&#x0D; Since professional culture is a complex concept that includes integrated personality traits, we have identified the levels of its formation

The Development of Digital Competence of Future Teachers in the Process of Distance Learning

The article digital competence is considered, on the one hand, as a component of professional competence, and on the other – as a level of personal digital culture. The structure of the studied phenomenon defines the motivational component (motivation to use digital technologies in professional activities, focus on collective interaction, the desire to succeed through the possession of information technology); cognitive component (level of knowledge on the application of digital technologies in professional activities); activity component (skills and abilities to use digital technologies for the organization of educational activities and solving professional problems); personal component (ability to self-study, professional self-development in a digital society).&#x0D; The pedagogical conditions for the development of digital competence of future teachers are substantiated: creation and maintenance of the digital environment of a higher education institution; activation of independent educational and cognitive activity of students by means of information and communication technologies; actualization of innovative directions and technologies of development of digital competence of future teachers in distance learning. The research was conducted with the students of the first (bachelor's) level of higher pedagogical education. The results of diagnostic sections have been generalized, statistical analysis of which testifies to the effectiveness of the developed system of development of digital competence of future teachers.

Beam delivery and final focus systems for multi-TeV advanced linear colliders

The Beam Delivery System (BDS) is a critical component of a high-energy linear collider. It transports the beam from the accelerator and brings it to a focus at the Interaction Point. The BDS system includes diagnostic sections for measuring the beam energy, emittance, and polarization, as well as collimators for machine protection. The length of the BDS increases with collision energy. Higher collision energies also require higher luminosities, and this is a significant constraint on the design for energy-frontier machines. Here, we review BDS designs based on traditional quadrupole magnets and examine the challenges involved in extending these to the Multi-TeV regime consistent with requirements for advanced accelerator concepts.

Factors influencing forming foresters' professionalismng foresters' professionalism

The article reveals the factors of formation of readiness of future forestry specialists for professional activity. The purpose of the study is to reveal the factors influencing the formation of the readiness of future forestry professionals for professional activities, in particular its criteria: motivational-personal, information-cognitive, effective-practical. Materials and methods: theoretical – analysis of psychological and pedagogical, scientific and methodological literature to reveal the factors influencing the formation of readiness of future forestry professionals for professional activities, justification of their importance for the formation of readiness criteria: motivational-personal, information-cognitive, effective-practical. External, internal and accidental factors contributing to the formation of readiness of future forestry specialists for professional activity are singled out. The group of external determinants includes: the dynamics of market development; the nature of the projected social order for higher professional education; change of educational paradigms; increasing the complexity of professional activity; development of management as a strategic management culture and professional field of activity, etc. The internal determinants that contribute to the formation of the readiness of future forestry professionals for professional activities include: subject-subject relations in the system "teacher-applicant"; value perception and development of applicants' orientation on their training; the presence of the need for professional activity in the course of training and the motives for its implementation; the desire of applicants to show their own internal (volitional) activity in the process of professional activity; level of readiness for professional activity; intention for creative professional activity in the context of formation of readiness for professional activity. Manifestation and use of random factors contribute to the formation of the readiness of future forestry professionals for professional activities, which depends on the teacher's ability to see a favorable case, a unique situation. The influence of internal factors on the formation of criteria for the readiness of future forestry professionals for professional activities: motivational-personal, information-cognitive, effective-practical. Effective diagnosis of professional and personal development of future forestry specialists allowed to obtain objective information about the state of formation of motivational and personal criteria, outline the main directions of work, determine methods and technologies for forming professional values of future professionals. Such diagnostics allowed to trace in dynamics the real efficiency of influence of various subjects of professional training on personal development of future experts, change of their attitude to realities of social and professional life. Carrying out diagnostic sections in the framework of research and experimental work allowed to obtain the necessary objective information about the state of information and cognitive criteria of readiness for professional activity, outline the main directions and content of this work, determine methods and technologies to provide emotional background for future forestry professionals. This diagnosis allowed us to trace the gradual dynamics of the evolution of information and cognitive criteria for the readiness of future professionals to work, to assess the real effectiveness of the influence of various factors on its development. Effective diagnosis of professional and personal development of future forestry specialists as a factor in the formation of their readiness for professional activity allowed to obtain the necessary information on the status of effective and practical criteria of professional readiness, outline main directions of work, identify methods and technologies activities. We see the prospect of further research in the studied influences of selected factors on the formation of the readiness of future forestry professionals to work.

FACTORS INFLUENCING FORMING FORESTERS' PROFESSIONALISM

The article reveals the factors of formation of readiness of future forestry specialists for professional activity. The purpose of the study is to reveal the factors influencing the formation of the readiness of future forestry professionals for professional activities, in particular its criteria: motivational-personal, information-cognitive, effective-practical. Materials and methods: theoretical – analysis of psychological and pedagogical, scientific and methodological literature to reveal the factors influencing the formation of readiness of future forestry professionals for professional activities, justification of their importance for the formation of readiness criteria: motivational-personal, information-cognitive, effective-practical. External, internal and accidental factors contributing to the formation of readiness of future forestry specialists for professional activity are singled out. The group of external determinants includes: the dynamics of market development; the nature of the projected social order for higher professional education; change of educational paradigms; increasing the complexity of professional activity; development of management as a strategic management culture and professional field of activity, etc. The internal determinants that contribute to the formation of the readiness of future forestry professionals for professional activities include: subject-subject relations in the system "teacher-applicant"; value perception and development of applicants' orientation on their training; the presence of the need for professional activity in the course of training and the motives for its implementation; the desire of applicants to show their own internal (volitional) activity in the process of professional activity; level of readiness for professional activity; intention for creative professional activity in the context of formation of readiness for professional activity. Manifestation and use of random factors contribute to the formation of the readiness of future forestry professionals for professional activities, which depends on the teacher's ability to see a favorable case, a unique situation. The influence of internal factors on the formation of criteria for the readiness of future forestry professionals for professional activities: motivational-personal, information-cognitive, effective-practical. Effective diagnosis of professional and personal development of future forestry specialists allowed to obtain objective information about the state of formation of motivational and personal criteria, outline the main directions of work, determine methods and technologies for forming professional values of future professionals. Such diagnostics allowed to trace in dynamics the real efficiency of influence of various subjects of professional training on personal development of future experts, change of their attitude to realities of social and professional life. Carrying out diagnostic sections in the framework of research and experimental work allowed to obtain the necessary objective information about the state of information and cognitive criteria of readiness for professional activity, outline the main directions and content of this work, determine methods and technologies to provide emotional background for future forestry professionals. This diagnosis allowed us to trace the gradual dynamics of the evolution of information and cognitive criteria for the readiness of future professionals to work, to assess the real effectiveness of the influence of various factors on its development. Effective diagnosis of professional and personal development of future forestry specialists as a factor in the formation of their readiness for professional activity allowed to obtain the necessary information on the status of effective and practical criteria of professional readiness, outline main directions of work, identify methods and technologies activities. We see the prospect of further research in the studied influences of selected factors on the formation of the readiness of future forestry professionals to work.

ДОСЛІДЖЕННЯ СХИЛЬНОСТІ ДО АГРЕСИВНОЇ ПОВЕДІНКИ СЕРЕД УЧНІВСЬКОЇ І СТУДЕНТСЬКОЇ МОЛОДІ В УМОВАХ НАДЗВИЧАЙНИХ СИТУАЦІЙ

The purpose of the article is to study the influence of emergency factors on the tenden-cy to aggressive behaviour among students. We conducted a study in Ukraine during 2016-2020 in emergency situations. In 2016, for the third year in a row, a military emergency situation con-tinued in eastern Ukraine, which left its mark on all the country's youth. In 2020, young people were exposed to a medical and biological emergency due to the Covid-19 pandemic. We made two diagnostic sections, respectively, in 2016 and 2020 using the same method of "Aggressive behaviour" (according to E. Ilin and P. Kovaliov). The results of the study show that there is a direct connection between a young person's stay in an emergency situation and an increase in the level of aggressive behaviour, in particular direct, physical one. Therefore, in emergency situations, it is necessary to develop and implement programs for the socio-psychological and pedagogical rehabilitation of young people.

Formation of an innovation-pedagogical actor in the school environment

Introduction. The need to develop and test a complex of psychological-pedagogical tools for the development of an innovation actor is due to the priorities of the national policy in the field of education, as well as the requirements for the teacher’s competencies in innovation, enshrined in the Professional Standard “Teacher”. Research purpose: the testing of a set of psychological-pedagogical tools for the development of an innovation actor, corresponding to the eco-psychological actor formation model. Materials and methods: during the 2017/2019 academic years, 78 students from schools in the city of Vladimir (Russian Federation) took part in the experimental verification of the eco-psychological model of innovation-pedagogical actor development. On diagnostic sections in the process of tools testing, the following methods were used: a method of determining the degree of manifestation of the organizational-educational models by V.A. Yasvin’s school, A.V. Kaptsov’s method to determine the degree of subjectivity manifestation at each stage of the eco-psychological model, a questionnaire “Personal Characteristics of a Professional” by I.G. Senin and V.E. Oryol, a test “Tolerance to Uncertainty” by D.А. Leontyev, a questionnaire “Creativity” by N.F. Vishnyakova. Research results. The set of psychological-pedagogical tools includes events (workshops, trainings, round tables) aimed at the formation of pedagogical subjectivity in accordance with the staging of the eco-psychological model (from an actor of perceptual activity to an actor of creative self-expression) and the organizational-modular organization of the educational space. The presented results reflect the positive dynamics of manifestation of subjectivity stages: “Master” (p&lt;0.05), “Creator” (p&lt;0.01) in the experimental sample, as well as a change in the manifestation of the innovation-modular organizational-educational model of the experimental school (U(26.40)=336.0, p=0.016). Differences (p&lt;0.05) were found in favor of the experimental school in terms of parameters characterizing creative thinking, open-mindedness, tolerance to uncertainty. It is shown that the eco-psychological model of innovation-pedagogical actor development is effective in the experimental school environment and allows for a systematic analysis of the current state and readiness of an educational organization for innovative changes. The developed set of psychological-pedagogical tools is the basis for the subsequent development of a system of scientific-methodological support of a school team as an innovation-pedagogical group actor.

Emotional Intelligence as a Factor against Burnout in Female Students and Teachers

A joint analysis of the concepts of "emotional intelligence" and "burnout" allows finding new ways to protect against the adverse effects of chronic stress. It is known that emotional competencies are determined by gender and gender, but this aspect needs to be clarified. A longitudinal study was conducted in a female sample (575 students and 96 teachers from different regions of Ukraine) to determine the dynamics of burnout in calm and stressful periods and trace the correlation of symptoms with emotional abilities. The structural components of emotional intelligence (reflection, self-regulation, empathy, expressiveness, and acceptance of one's own emotions) and manifestations of burnout were measured; three diagnostic sections were made at the beginning of the academic year, after the winter and spring examination sessions. Significant growth of all burnout indicators during the annual training cycle (MANOVA) was recorded. The effect of the accumulation of fatigue (exhaustion) was robust in teachers and graduate students. At the same time, after the session, students grew a sense of self-efficacy, compensating for the resources spent. Comparison of means in six subsamples of students in grades 1-5 and teachers (ANOVA) showed that structural changes in emotional competencies describe the adaptive potential of a certain age period. The developed empathy and self-regulation are the main signs of women's emotional maturity after graduation. The structure of correlations between burnout parameters and emotional competencies differed in different groups. The most significant contribution to preventing burnout in students is made by reflection and self-regulation, in teachers — by self-regulation, empathy, and acceptance of one's feelings. The negative correlation between emotional abilities and symptoms of burnout is exacerbated during times of stress. It is concluded that there are two mechanisms of the protective influence of emotional intelligence: direct, which prevents exhaustion by controlling and regulating negative emotions, and indirect, through a sense of self-efficacy resulting from the successful overcoming of professional challenges.

Emerging issues and preventive measures for health care workers in the diagnostic field in coronavirus disease 2019 pandemic

Coronavirus disease 2019 caused by severe acute respiratory syndrome-CoV-2 is an emerging infectious disease first identified in Wuhan City, Hubei Province, China, which subsequently spread as a global pandemic posing a global threat. As per World Health Organisation worldwide around 38 lac people have been infected and 2.6 lac people have died from the disease and in India 59,662 people are infected with 1981 deaths by May 9, 2020. It is feasible that potentially infectious specimens may be submitted in our laboratory those are the impending source of infection to the laboratory personnel. Using imaging equipment on coronavirus confirmed/suspected patient/carriers is a serious hazard for healthcare providers because there is a risk that the virus could remain on the surface of the computed tomography scan machines or ultrasound (US) probes/patient bed/couch. Here, we have enlisted the rigorous biosafety measures which if executed appropriately can significantly drop the chances of spread of infection to health care workers in these diagnostic sections.

Spiritual Culture Formation of Young Teachers in the System of Higher Education: The Way for Social Maturity Development

The formation of spiritual culture should be based on the principles that guide every teacher in the implementation of their pedagogical skills in direct learning. The purpose of our study is to substantiate that young teachers' social maturity is characterized not only by the level of knowledge, development of their orientations but also by the degree of personal spiritual culture. We have applied the method of diagnosing a level of young teacher's social maturity development as a moderator in the search for spiritual values, but also a participant in the formation of personal spiritual culture directly at the student himself. Diagnostic sections have shown that the index of general social maturity of young teachers who shape the spiritual culture of students, is growing much faster compared to the young teachers who didn't show the activity of this kind. From the experimental data, it follows that young teachers' activity in creating a pedagogical environment of their school is an effective factor in the intensification of their social maturity formation. Investigating the problem of the formation of social maturity of the individual, a certain system of personal orientations develops, which serves as the basis of spiritual culture, which the student can choose. The authors show that such an opportunity can only be implemented with the teacher's integrated participation as a carrier of moral values. The study's novelty is determined by the fact that each of the participants in the educational process seeks to realize the share and type of moral views for the fulfillment of their personal interests. The study's practical significance is determined by the fact that spiritual culture can be based not only on its private but also on its public perception.