Diagnosis Of Cervical Diseases Research Articles

Compared between support vector machine (SVM) and deep belief network (DBN) for multi-classification of Raman spectroscopy for cervical diseases

In this study, a minimally invasive test method for cervical cancer in vitro was proposed by comparing Raman spectroscopy with support vector machine (SVM) model and deep belief network (DBN) model. The serum Raman spectra of cervical cancer, hysteromyoma, and healthy people were collected. After data processing, SVM classification model and DBN classification model were built respectively. The experimental results show that when the DBN network algorithm is used, the sample test set can be divided accurately and the result of cross-validation is ideal. Compared with the traditional SVM algorithm, this method firstly screened the effective feature matrix from the data, and then classified the data. With high efficiency and accuracy, based on 445 samples collected, this method improved the accuracy by 13.93%±2.47% compared with the SVM method, and provided a new direction and idea for the in vitro diagnosis of cervical diseases.

Importance of Cytological Screening in the Diagnosis of Cervical Diseases

The purpose of the study was to compare the conventional Pap smear with liquid-based cytology in the early diagnosis of cervical diseases. Materials and methods. The study included 150 women between the ages of 18 and 73 with cervical diseases. The comparison was held on the basis of the results of histology of liquid-based and conventional Pap smears taken from cervix. Bethesda classification was used to make the diagnosis. Diagnostic performance was calculated in terms of sensitivity, specificity, positive predictive value and negative predictive value. Results and discussion. During the sensitivity, specificity and prognostic assessment of liquid-based cytology with conventional Pap smears, the sensitivity of liquid-based cytology was higher than the conventional Pap test – 93.1%, and the conventional Pap test was 81.3%. The specificity of liquid-based cytology can be compared with a conventional Pap test (76.2% and 70.6%, respectively). The positive prognostic value was 84.4% in liquid-based cytology and 88.6% in the conventional Pap test. Negative prognostic value was significantly higher in liquid-based cytology than in conventional Pap tests (88.9% and 57.1%, respectively). The total diagnostic value was 86.0% in liquid-based cytology and 78.5% in the conventional Pap test. As a result of the study it has become clear that liquid-based cytology is an appropriate method for the diagnosis of cervical diseases. There are screening programs for cervical, breast, colorectal and prostate cancer in the country, but due to some psycho-social factors, restrictions and barriers, patients only seek medical attention when there is an urgent need. As a result, more than half of all cancers are diagnosed at a late stage. Thus, the study concluded that liquid-based cytology is more convenient than conventional smear screening for cervical cancer screening. As single-layer smears are easier to examine, cells with atypia are not covered by other cells (inflammation, blood, etc.). In addition, the amount of unsatisfactory smears is minimal. In general, many studies have been conducted comparing liquid-based cytology with conventional Pap smears. The results were different in both the initial studies and the meta-analysis. Conclusion. Thus, both screening methods predict the likelihood of disease in the same way, but with liquid-based cytology, the number of false-negative results is less, and the sample quality is improved by reducing the number of unsatisfactory smears. Also, women with liquid-based cytology are more likely to get a positive result than those with cervical disease. Liquid-based cytology is superior and more sensitive than conventional Pap tests in the detection of cervical neoplasms

Microwave Imaging for the Diagnosis of Cervical Diseases: A Feasibility Analysis

An inverse scattering method working at microwave frequencies for cervical diagnostics is proposed in this work. The aim is the diagnosis of cervical myelopathy, which is a disease that affects the first part of the spinal cord (between the C3 and C7 vertebra). A preliminary feasibility analysis oriented toward the development of an imaging system is reported. The system prototype includes a set of antennas that illuminate the neck and retrieve samples of the scattered electric field. The related inverse scattering problem is solved by using a nonlinear Newton-type reconstruction procedure, which provides two-dimensional images of the dielectric parameters of a neck cross section. A simplified cylindrical phantom mimicking the human neck has been designed for assessing the feasibility of the envisioned microwave measurement system and processing technique. Numerical results are reported to evaluate the capabilities of the proposed approach. Moreover, initial experimental results have been obtained by using cylindrical containers and a 3D printed version of the developed neck phantom.

Biomarkers of cervicovaginal fluid for the diagnosis of cervical diseases associated with human papilloma virus (literature review)

Human papillomavirus (HPV) is one of the main pathogens of genital tract infections, which can lead to malignant transformation of cells of the cervix, vagina, vulva and anus. Approximately 90% of cervical intraepithelial neoplasia cases and 99% of cervical cancer cases occur in HPV-positive patients. However, the presence of HPV in the patients body can not be considered as a marker of progression or regression of the pathological process. The need to determine further tactics of examination and management of women with HPV-associated diseases of the cervix States the search for molecular genetic markers of neoplastic transformation in order to predict the risk of cervical neoplasia and cervical cancer. Diagnostic systems based on determination of biomarkers in cervicovaginal fluid (CVJ) can become convenient for application in clinical practice. The panel of CVJ proteins allows to make the exact characteristic of a condition of bodies of female reproductive system, including at neoplastic processes of a cervix of a uterus. The biomarkers identified in the CVJ can be used as informative tests to improve the accuracy of diagnosis of pathological changes in the cervix and to determine the risk criteria for the development of malignancy.

Liquid cytology in comparison with traditional in the diagnosis of cervical diseases

Liquid cytology in comparison with traditional in the diagnosis of cervical diseases

Computer-Aided Diagnosis of Label-Free 3-D Optical Coherence Microscopy Images of Human Cervical Tissue.

Objective:Ultrahigh-resolution optical coherence microscopy (OCM) has recently demonstrated its potential for accurate diagnosis of human cervical diseases. One major challenge for clinical adoption, however, is the steep learning curve clinicians need to overcome to interpret OCM images. Developing an intelligent technique for computer-aided diagnosis (CADx) to accurately interpret OCM images will facilitate clinical adoption of the technology and improve patient care.Methods:497 high-resolution 3-D OCM volumes (600 cross-sectional images each) were collected from 159 ex vivo specimens of 92 female patients. OCM image features were extracted using a convolutional neural network (CNN) model, concatenated with patient information (e.g., age and HPV results), and classified using a support vector machine classifier. Ten-fold cross-validations were utilized to test the performance of the CADx method in a five-class classification task and a binary classification task.Results:An 88.3±4.9% classification accuracy was achieved for five fine-grained classes of cervical tissue, namely normal, ectropion, low-grade and high-grade squamous intraepithelial lesions (LSIL and HSIL), and cancer. In the binary classification task (low-risk [normal, ectropion and LSIL] vs. high-risk [HSIL and cancer]), the CADx method achieved an area-under-the-curve (AUC) value of 0.959 with an 86.7±11.4% sensitivity and 93.5±3.8% specificity.Conclusion:The proposed deep-learning based CADx method outperformed four human experts. It was also able to identify morphological characteristics in OCM images that were consistent with histopathological interpretations.Significance:Label-free OCM imaging, combined with deep-learning based CADx methods, hold a great promise to be used in clinical settings for the effective screening and diagnosis of cervical diseases.

Ранняя диагностика заболеваний шейки матки

Ранняя диагностика заболеваний шейки матки

Clinicopathological significance of atypical squamous epithelium cells which cannot exclude high-grade squamous intraepithelial lesions in cervical cytology

Objective To investigate clinicopathological significance of atypical squamous epithelium cells which cannot exclude high grade squamous intraepithelial lesions (ASC-H) in the diagnosis of cervical diseases. Methods The results of age, high-risk human papillomavirus DNA (hrHPV DNA) and cervical biopsy in 496 patients with ASC-H from March 2012 to December 2015 in Shanxi Dayi Hospital were analyzed. Results Among 496 ASC-H cases, the proportion of the patients between 40 and 49 years old was the highest [30.8% (153/496)]. HrHPV DNA was detected in 154 cases, and the positive rate was 79.2% (122/154), and the positive rate of patients at the age of 18 to 29 years old was the highest [84.2% (16/19)]. The detection rate of cervical intraepithelial neoplasia (CIN)Ⅱand above lesions in 124 cases with cervical biopsy was 66.9% (83/124), including 100 cases with hrHPV DNA positive (80.6%) and 24 cases with hrHPV DNA negative (19.4%). There were 71 cases (71.0%) of CINⅡ, CIN Ⅲ, early squamous carcinoma and squamous cell carcinomas in 100 hrHPV DNA positive patients with cervical biopsy. There were 12 cases (50.0%) of CINⅡ/Ⅲ changes in 24 hrHPV DNA negative patients with cervical biopsy, but none in early squamous carcinoma and squamous cell carcinomas was detected, and there was a significant difference between hrHPV DNA positive and negative patients (χ2=3.857, P < 0.05). The positive predictive value, negative predictive value, sensitivity and specificity of hrHPV DNA detection for diagnosis of CINⅡand above lesions were 85.5%, 29.3%, 71.0% and 50.0%, respectively. Conclusions ASC-H strongly predicts CINⅡand above lesions in cervical cytology. The detection of hrHPV DNA has a high positive predictive value for CINⅡand above lesions. Key words: Cervical lesion; Atypical squamous epithelium cells, cannot exclude high grade squamous intraepithelial lesions; High-risk human papillomavirus DNA; Biopsy

Early diagnosing of cervical diseases

Purpose of the study: to increase the effectiveness of diagnosis of cervical disease. Materials and methods. To achieve this goal, 170 women were examined, 120 of them with cervical disease and 50 with no cervical disease. All patients underwent cytological examination of scrapes from the cervix, expanded colposcopy, infrared spectrometry and radio wave cervical excision (if indicated); a complex of methods for laboratory diagnosis of genital infections; HPV examination (human papilloma virus) by polymerase chain reaction, including HPV typing, viral load detection. Infrared spectrometry is a mathematical model of the disease, based on changes in the peaks of absorption bands of protein structures. Changes in the structure of the protein can be easily detected in the IR spectrum and used as molecular markers to determine the pathological state of the tissues. All patients underwent spectrometric examination of blood serum. When comparing the results with verified diagnoses, three-dimensional diagnostic IR spectrometric criteria were obtained in the form of polyhedron in the orthogonal coordinate system (X, Y, Z) for women with no cervical disease and with cervical disease (reactive changes in ASC cells that include ASC -US and ASC-H, CIN I, CIN II and CIN III). Results. Complexes of characteristic absorption spectra of blood serum in the diagnosis of cervical diseases were determined. The sensitivity of IR spectrometry (95%) in the diagnosis of cervical dysfunction, which is superior to that for cytological (67%) and colposcopic (63%) research methods, was established. Diagnostic accuracy of infrared spectrometry of blood serum is 90%, specificity is 92%. Conclusions. The developed method of diagnosing cervical diseases is recommended as a method of screening diagnostics in women's clinics and gynecological hospitals. The method will significantly shorten the time of examination of patients, improve the diagnosis of cervical disease, exclude unreasonable surgical treatment and obtain a significant economic effect.

CERVICAL DISEASES

Objectives: The aim of our study is to compare the efficacies of Pap smearcytology, colposcopy and histopathology, in diagnosis of cervical diseases. Study Design:Cross sectional cohort study. Period: One year from June 2013 to June 2014. Setting:Tertiary care hospital in Karachi, Pakistan. Method: The patient population n=145 consistedof women ranging from age 18 to 80 years old, who had come to the outpatient clinics for avariety of symptoms. Pap smear, colposcopy and histopathology was done for women who hadsymptoms like recurrent vaginal discharge, postcoital bleeding and other diseases of the cervix.Findings were noted on a proforma and all the patients signed a duly informed consent. Datawas analyzed using SPSS version 23. Results: The mean age was 45 +/- 9 years (between24 – 75 years), the parity was 5 +/- 4, normal colposcopy findings were found in n= 66 women,while abnormal findings were found in n=79 women. In the abnormal colposcopic finding groupn=63 had abnormal histopathology (p&lt;0.001) abnormal histopathology was found in n=11women who had normal colposcopic findings (16.67% false negative), the calculated sensitivityand specificity of colposcopy was determined to be 86% and 79%. For pap smear abnormalitywas found in n= 49 patients, out of which n=29 had abnormal findings on histopathology aswell. And for colposcopy and pap smear out of n= 78 patients who had abnormal colposcopicfindings, abnormal pap smear was found in only n= 28 patients. And out of those who hadnormal colposcopy n= 67, n= 21 had abnormal pap smear (False positivity of 31.34%) similarlyout of those who had normal pap smear n= 96, n= 44 had abnormal histopathology (falsenegativity of 45.83%). The calculated sensitivity and specificity of pap smear was 38.8% and71.8% respectively. Conclusion: We have concluded that there is a strong correlation betweenfindings of colposcopy and histopathological diagnosis, but when it comes to the diagnosismade by pap smear and colposcopy and pap smear and histopathology the correlation is weakand not consistent.

English

Objectives: The aim of our study is to compare the efficacies of Pap smearcytology, colposcopy and histopathology, in diagnosis of cervical diseases. Study Design:Cross sectional cohort study. Period: One year from June 2013 to June 2014. Setting:Tertiary care hospital in Karachi, Pakistan. Method: The patient population n=145 consistedof women ranging from age 18 to 80 years old, who had come to the outpatient clinics for avariety of symptoms. Pap smear, colposcopy and histopathology was done for women who hadsymptoms like recurrent vaginal discharge, postcoital bleeding and other diseases of the cervix.Findings were noted on a proforma and all the patients signed a duly informed consent. Datawas analyzed using SPSS version 23. Results: The mean age was 45 +/- 9 years (between24 – 75 years), the parity was 5 +/- 4, normal colposcopy findings were found in n= 66 women,while abnormal findings were found in n=79 women. In the abnormal colposcopic finding groupn=63 had abnormal histopathology (p&lt;0.001) abnormal histopathology was found in n=11women who had normal colposcopic findings (16.67% false negative), the calculated sensitivityand specificity of colposcopy was determined to be 86% and 79%. For pap smear abnormalitywas found in n= 49 patients, out of which n=29 had abnormal findings on histopathology aswell. And for colposcopy and pap smear out of n= 78 patients who had abnormal colposcopicfindings, abnormal pap smear was found in only n= 28 patients. And out of those who hadnormal colposcopy n= 67, n= 21 had abnormal pap smear (False positivity of 31.34%) similarlyout of those who had normal pap smear n= 96, n= 44 had abnormal histopathology (falsenegativity of 45.83%). The calculated sensitivity and specificity of pap smear was 38.8% and71.8% respectively. Conclusion: We have concluded that there is a strong correlation betweenfindings of colposcopy and histopathological diagnosis, but when it comes to the diagnosismade by pap smear and colposcopy and pap smear and histopathology the correlation is weakand not consistent.

Quantitative detection of APC/RASSF1A promoter methylation in the plasma of patients with cervical diseases

To quantitatively detect adenomatous polyposis coli(APC) and Ras association domain family 1A( RASSF1A) promoter methylation levels in the plasma of patients with cervical disease and to determine the diagnostic value of the indicators of cervical disease. Preoperative blood samples were collected from 25 cases of healthy women and 118 cases of cervical disease, and tissue samples were also collected from 31 cases of them. The APC/RASSF1A promoter methylation levels of plasma and tissue were determined by duplex real-time quantitative methylation specific PCR (qMSP). Among 31 paired plasma and tissue samples, true negative rate of APC and RASSF1A genes were all 100%, and true positive rate of APC and RASSF1A genes were 3/5 and 7/9, respectively. In 143 cases of plasma samples, total positive rate of APC and (or) RASSF1A methylation was 3% (2/59) for control/low-grade lesions groups and 48% (40/84) for high-grade lesions/tumor groups (P < 0.01) . RASSF1A methylation rate was related to lymph node metastasis and International Federation of Gynecology and Obstetrics (FIGO) stage (P < 0.05). The plasma APC/RASSF1A methylation detection may be with some application prospect in the diagnosis of cervical diseases.

P20.03: The value of puncture biopsy guided by transvaginal ultrasound in the diagnosis of cervical diseases

<scp>P20</scp>.03: The value of puncture biopsy guided by transvaginal ultrasound in the diagnosis of cervical diseases

Study of quantitative detection of circulating DNA in the plasma of patients with cervical lesion

To quantitatively detect circulating DNA levels in the plasma of patients withcervical lesion and to determine the value for diagnosis of cervical lesion and cervical cancer. Preoperative blood samples were collected from 53 cases of low-grade lesions, 49 cases of high-grade lesions, 44 cases of cervical invasive cancer and 70 cases of healthy women. Plasma DNA was extracted by magnetic bead method (BILATEST DNA kit). The quantity of plasma DNA was determined by duplex real-time quantitative PCR. Median plasma DNA level of invasive cervical cancer patients was 61.59 mg/L (32.06-162.16 mg/L), which was significantly higher than that of healthy women [16.35 mg/L (11.98-22.71 mg/L), P<0.01]. Among invasive cervical cancer patients, median plasma DNA level of squamous carcinoma patients was slightly higher than that of adenocarcinoma (50.43 versus 47.31 mg/L, P>0.05). Median plasma DNA level of stage I patients was lower than that of stage II-III patients (46.02 versus 71.35 mg/L, P<0.05). Quantitatively detecting plasma circulating DNA may be with some application prospect in the diagnosis of cervical diseases.

Clinical study on cervix biopsy guided by colposcopy in diagnosis of cervical diseases in pregnant women

To investigate the value and safety of biopsy guided by colposcopy in diagnosis of cervical diseases in pregnant women. From Aug. 2007 to Feb. 2009, 17 828 pregnant women who receive antenatal examination underwent cervical cytological screening thinprep cytology test (TCT) in Beijing Obstetrics and Gynecology Hospital. If abnormal cytological results were found, those pregnant women were administered by colposcopic examination and biopsy after they signed informed consent. (1) TCT: the abnormal TCT results of 1502 pregnant women (8.425%, 1502/17 828) were found in 17 828 cases. (2) Colposcopic examination: two hundred and four pregnant women underwent colposcopic examination. The rate of satisfied colposcopic imaging was 92.6% (189/204), colposcopic examination identified 125 cases with cervical inflammation or cervical intraepithelial neoplasia (CIN)I, 25 cases with CINII and 54 cases with CINIII or microinvasive squamous carcinoma (MIVC) of squamous cervical carcinoma (SCC). (3) The results of biopsy guided by colposcopy: among 204 cases, it was found 33 cases with cervical inflammation or wart, 95 cases with CINI, 28 CINII, 36 cases with CINIII and 12 cases with MIVC. (4) The rate of concordance: compared with biopsy pathologic examination, colposcopy examination found 113 cases with cervical inflammation and CINI, the rate of concordance was 90.4% (113/125). And 54 cases with CINIII or SCC diagnosed by colposcopy examination, however biopsy pathologic examination confirm 23 cases with CINIII and 10 cases with SCC at stage Ia, the concordance rate was 61% (33/54). (5) Complication: eight (3.9%, 8/204) pregnant women underwent cervical wound suturing due to continuous bleeding after colposcopy exam or biopsy. No other complications were recorded. It is necessary that TCT should be performed in pregnant women without cytological screening within one year. Colposcopic examination and biopsy were indicated if pregnant woman with abnormal cytological result were found. Pregnant women with cervicitis or CINI diagnosed by colposcopy should be followed up. If pregnant woman was suspected with CINII or advanced disease, biopsy guided by colposcopy should be performed.

Fluid-based ThinPrep cytology teat in the diagnosis of cervical diseases

Objective To explore the value of ThinPrep cytology test (TCT) in the screening and diagnosis of cervical diseases. Methods 330 patients were detected by Pap smear and fluid-based TCT. Those with positive cytological results (ASCUS or above) received colposcopic biopsies. The findings of biopsy were compared with that of the cytological test. Results The rate of abnormal epithelial cells identified by TCT was significantly higher than that of Pap smear (23.94% vs. 16.06%, P<.0.05). And the rate of severe cellular abnormity detected by TCT increased by 42.92%. With colposcopy-guided biopsies as the gold standard, the positive coincidence rate of TCT was significantly higher than that of Pap smear (67.09% vs. 45. 28%, P< 0.05). Conclusions ThinPrep cytology test is more sensitive than pap smear in screening cervical diseases, and TCT combined with colposcopy-guided biopsies may increase the accuracy of diagnosis. Key words: ThinPrep cytology test; Pap smear, Cervical biopsy; Cervical diseases

The Value of the Tranditional Microscopic Pap's Smears in the Diagnosis of Cervical Diseases: Analysis of 12886 cases

目的 评价巴氏涂片对宫颈病变的诊断价值.方法 回顾性分析12886例患者的宫颈巴氏涂片结果,并结合阴道镜检查及组织病理活检,以病理活检结果为金标准进行对比分析.结果 12886例普查患者中,阳性(巴氏Ⅲ～Ⅴ级)病例为945例,阳性率为7.33%.945例宫颈巴氏涂片阳性患者中,有832位患者回院复诊并成功对其进行阴道镜检查下组织病理活检.组织病理活检阳性(HPV感染或CIN1以上)共634例,总阳性率为76.20%.其中HPV感染或CIN1为414例,占49.76%.CIN2为134例,占16.11%.CIN3为57例,占6.85%.宫颈癌29例,占3.49%.结论 宫颈巴氏涂片作为一种简便、快捷、经济、有效的筛查方法在现在医疗实践中仍不失为一种较适当的宫颈病变的筛查手段, 与阴道镜检查及组织病理活检相结合能明显提高结果的准确率。