Dextran Solution Research Articles

Reliable deep learning in anomalous diffusion against out-of-distribution dynamics.

Anomalous diffusion plays a crucial rule in understanding molecular-level dynamics by offering valuable insights into molecular interactions, mobility states and the physical properties of systems across both biological and materials sciences. Deep-learning techniques have recently outperformed conventional statistical methods in anomalous diffusion recognition. However, deep-learning networks are typically trained by data with limited distribution, which inevitably fail to recognize unknown diffusion models and misinterpret dynamics when confronted with out-of-distribution (OOD) scenarios. In this work, we present a general framework for evaluating deep-learning-based OOD dynamics-detection methods. We further develop a baseline approach that achieves robust OOD dynamics detection as well as accurate recognition of in-distribution anomalous diffusion. We demonstrate that this method enables a reliable characterization of complex behaviors across a wide range of experimentally diverse systems, including nicotinic acetylcholine receptors in membranes, fluorescent beads in dextran solutions and silver nanoparticles undergoing active endocytosis.

High precision acoustofluidic synthesis of stable, biocompatible water-in-water emulsions

Water-in-water (w/w) emulsions, comprising aqueous droplets within another continuous aqueous phase, rely on a low interfacial tension for stability. Thus far, it has been challenging to control their size and stability without the use of stabilizers. In this study, we introduce a microfluidic technique that addresses these challenges, producing stable w/w emulsions with precisely controlled size and uniformity. Results shows that using an acoustically actuated microfluidic mixer, PEG, Dextran, and alginate solutions (84.66 mPa.s viscosity difference) were homogenized rapidly, forming uniformly distributed w/w emulsions stabilized in alginate gels.The emulsion size, uniformity, and shear sensitivity can be tuned by modifying the alginate concentration. Biocompatibility was evaluated by monitoring the viability of kidney cells in the presence of emulsions and gels. In conclusion, this study not only showed emulsion formation with a high mixing efficiency exceeding 90 % for all viscosities, actuated at an optimized frequency of 1.064 MHz, but also demonstrated that an aqueous, solvent, and emulsifier-free composition exhibited remarkable biocompatibility, holding promise for precise drug delivery, cosmetics, and food applications.

Thermo-responsive aqueous two-phase system for two-level compartmentalization

Hierarchical compartmentalization responding to changes in intracellular and extracellular environments is ubiquitous in living eukaryotic cells but remains a formidable task in synthetic systems. Here we report a two-level compartmentalization approach based on a thermo-responsive aqueous two-phase system (TR-ATPS) comprising poly(N-isopropylacrylamide) (PNIPAM) and dextran (DEX). Liquid membraneless compartments enriched in PNIPAM are phase-separated from the continuous DEX solution via liquid-liquid phase separation at 25 °C and shrink dramatically with small second-level compartments generated at the interface, resembling the structure of colloidosome, by increasing the temperature to 35 °C. The TR-ATPS can store biomolecules, program the spatial distribution of enzymes, and accelerate the overall biochemical reaction efficiency by nearly 7-fold. The TR-ATPS inspires on-demand, stimulus-triggered spatiotemporal enrichment of biomolecules via two-level compartmentalization, creating opportunities in synthetic biology and biochemical engineering.

Facile compliance-based pump for blood physiometer

The biomechanical properties of blood are considered promising label-free biomarkers for early disease detection. Disposable pumps have been suggested as replacements for bulky and expensive syringe pumps. However, they have limitations, including initial air bubble removal, simple stop-and-run flow control, and quantification of many rheological properties. In this study, a compliance-based pump (CP) is developed by fitting a blood-loaded syringe, an air-compliance unit, and a needle into each port of a three-way valve. When blood is loaded into the microfluidic channel from the CP, the initial air bubbles are removed from the channels. By manipulating the three-way valve, blood flow is stopped immediately. Red blood cell (RBC) aggregation index (AI) is obtained by analyzing microscopic blood images. The air-compliance unit induces a transient blood flow. The time-resolved micro-particle image velocimetry technique is employed to obtain the blood velocity. The flow rate and time constant are obtained by assuming the flow rate as Q (t) = Q1 exp(−t/λ1) + Q2 exp(t/λ2) and conducting nonlinear regression analysis. The proposed method is employed to obtain the properties (AI, Q1, Q2, λ1, and λ2) of suspended bloods (hematocrit = 30%–70%, 5–30 mg/ml dextran solution, and heat-shocked RBCs). It is also used to detect four different types of suspended blood prepared by adding two types of RBCs (normal or hardened RBCs) to two types of diluents (1 × phosphate-buffered saline or dextran). In conclusion, the proposed method can be used to detect differences in suspended blood by manipulating the CP and consistently analyzing microscopic blood images.

Acoustical Analysis of Dextran+urea: Insights into Molecular Interactions

Introduction:: The aim of this study is to determine the acoustic parameters of polymer dextran with urea. Recent years have seen an increase in the use of ultrasonic research to describe the physiochemical and thermodynamic characteristics of liquid solutions at various temperatures and frequencies. The size of the pure component and the mixtures had an impact on various interactions, molecular mobility, and kinds of interaction. Which were studied using the acoustical and thermodynamic characteristics? To ascertain how the solvent urea interacts with the solute dextran at the molecular level. Materials and Method: Dextran (molecular weight of 70000) with 6(M)urea was used. The solution's density using a pycnometer, viscosity using an Ostwald viscometer, and ultrasonic velocity using an ultrasonic interferometer were examined. Results:: The physical properties of the medium are affected by the transmission of ultrasonic waves, which also teaches us about the physics of liquids and solutions. Understanding the interactions between the solutes and the solvent in the solution of dextran and urea, both the evaluated parameters were used, such as free volume, internal pressure, absorption coefficient, Rao's constant, and Wada's constant, as well as the observed values, such as ultrasonic velocity, density, and viscosity. Conclusion:: Based on the modification of these parameters with varied temperature and frequency, molecular mobility, different types of intermolecular interaction, and the strength of the bond between the solute (dextran 0.5%) and solvent (6(M) urea) are investigated. The findings have been explained in terms of a structural reorganisation in the aqueous dextran solution. At all the temperatures used for the investigation, the solute-solvent interactions are more significant. The change in the acoustic properties is small because the frequency variation causes the molecules to move swiftly and have little chance to interact. Investigating molecular interactions, including electrostriction, acceptor-donor association, dipole-dipole association, and hydrogen bonding, has used these properties. Understanding molecular interactions helps one to comprehend the core issues surrounding the mechanisms of chemical and biological catalysis and the routes of chemical reactions.

3D structured capillary cell suspensions aided by aqueous two-phase systems.

We report a facile technique for 3D structuring of living cells by forming capillary cell suspensions based on an aqueous two-phase system (ATPS) of polyethylene glycol (PEG) and dextran (DEX) solutions. We demonstrate the formation of water-in-water (DEX-in-PEG) capillary bridges using concentrated suspensions of yeast cells which show enhanced rheological properties and distinctive 3D patterns. Capillary structured cell suspensions can potentially find applications in novel ways of 3D cell culturing, instant tissue engineering and many biomedical investigations.

Dextran of Diverse Molecular-Configurations Used as a Blood-Plasma Substitute, Drug-Delivery Vehicle and Food Additive Biosynthesized by Leuconostoc, Lactobacillus and Weissella

Dextran, a microbial metabolite of diverse molecular configurations, can be biosynthesized employing selected strains of characterized species of bacteria. Dextran molecules are secreted as an extracellular polysaccharide in the culture medium of the bacterial fermentation system. This microbially produced polymer of glucose possesses multi-faceted characteristics such as its solubility in different solvents and formation of dextran solutions of needed viscosity. Several preparations can be formulated for the desired thermal and rheological properties. Due to such multifunctional characteristics, dextran with different structural specifications is a desired polysaccharide for clinical, pharmaceutical, and food industry commercial applications. Dextran and its derivative products with various molecular weights, in a range of high and low, have established their uses in drug delivery and in analytical devices using columns packed with polysaccharide gel. Therefore, being a neutral raw material, the resourcefulness of dextran preparations of different molecular weights and linkages in their polymer configuration is important. For this purpose, several studies have been performed to produce this commercially important polysaccharide under optimized bacterial cultivation processes. This article aims to overview recently published research reports on some significant applications of dextran in the pharmaceutical and food industries. Studies conducted under optimized conditions in fermentation processes for the biosynthesis of dextran of diverse molecular configurations, which are responsible for its multifunctional properties, have been summarized. Concise information has been presented in three separate tables for each group of specific bacterial species employed to obtain this extracellular microbial polysaccharide.

Effect of sodium nitroprusside on the microrheological properties of red blood cells in different media

Red blood cell (RBC) aggregation as well as their deformation significantly affects blood microrheology. These processes depend on various factors, one of which is concentration of the nitric oxide, one of the main signaling molecule in the bloodstream. The purpose of this study was to investigate the effect of nitric oxide on the microrheological properties of red blood cells (RBCs) in RBC samples of various media after the addition of nitric oxide donor sodium nitroprusside in vitro. Microrheological properties were measured using laser aggregometer and ektacytometer based on diffuse light scattering and diffraction of laser light on a suspension of RBCs, respectively. The study found that heparin-stabilized blood showed increased RBC aggregation and deformation with sodium nitroprusside concentrations of 100, and 200[Formula: see text][Formula: see text]M, while EDTA-stabilized blood showed slightly decreased aggregation and unchanged deformation. With washed RBCs in dextran solution, the addition of sodium nitroprusside (in the concentrations of 100, and 200[Formula: see text][Formula: see text]M) resulted in decreased aggregation and increased deformation. These findings aid in our understanding of nitric oxide’s effect on RBC microrheological properties.

The Impact of COVID-19 on Cellular Factors Influencing Red Blood Cell Aggregation Examined in Dextran: Possible Causes and Consequences.

Several studies have indicated that COVID-19 can lead to alterations in blood rheology, including an increase in red blood cell aggregation. The precise mechanisms behind this phenomenon are not yet fully comprehended. The latest findings suggest that erythrocyte aggregation significantly influences microcirculation, causes the formation of blood clots in blood vessels, and even damages the endothelial glycocalyx, leading to endothelial dysfunction. The focus of this research lies in investigating the cellular factors influencing these changes in aggregation and discussing potential causes and implications in the context of COVID-19 pathophysiology. For this purpose, the aggregation of erythrocytes in a group of 52 patients with COVID-19 pneumonia was examined in a 70 kDa Dextran solution, which eliminates the influence of plasma factors. Using image analysis, the velocities and sizes of the formed aggregates were investigated, determining their porosity. This study showed that the process of erythrocyte aggregation in COVID-19 patients, independent of plasma factors, leads to the formation of more compact, denser, three-dimensional aggregates. These aggregates may be less likely to disperse under circulatory shear stress, increasing the risk of thrombotic events. This study also suggests that cellular aggregation factors can be responsible for the thrombotic disorders observed long after infection, even when plasma factors have normalized. The results and subsequent broad discussion presented in this study can contribute to a better understanding of the potential complications associated with increased erythrocyte aggregation.

ACOUSTIC AND THERMODYNAMICS STUDY OF AQUEOUS DEXTRAN: AN ULTRASONIC ANALYSIS

The acoustical investigation of molecular interaction has been performed from the measurement parameters of ultrasonic velocity (U), density (ρ) and viscosity (η) of aqueous dextran solution at different temperatures. The thermodynamic and other allied pa

Inducing an LCST in hydrophilic polysaccharides via engineered macromolecular hydrophobicity

Thermoresponsive polysaccharide-based materials with tunable transition temperatures regulating phase-separated microdomains offer substantial opportunities in tissue engineering and biomedical applications. To develop novel synthetic thermoresponsive polysaccharides, we employed versatile chemical routes to attach hydrophobic adducts to the backbone of hydrophilic dextran and gradually increased the hydrophobicity of the dextran chains to engineer phase separation. Conjugating methacrylate moieties to the dextran backbone yielded a continuous increase in macromolecular hydrophobicity that induced a reversible phase transition whose lower critical solution temperature can be modulated via variations in polysaccharide concentration, molecular weight, degree of methacrylation, ionic strength, surfactant, urea and Hofmeister salts. The phase separation is driven by increased hydrophobic interactions of methacrylate residues, where the addition of surfactant and urea disassociates hydrophobic interactions and eliminates phase transition. Morphological characterization of phase-separated dextran solutions via scanning electron and flow imaging microscopy revealed the formation of microdomains upon phase transition. These novel thermoresponsive dextrans exhibited promising cytocompatibility in cell culture where the phase transition exerted negligible effects on the attachment, spreading and proliferation of human dermal fibroblasts. Leveraging the conjugated methacrylate groups, we employed photo-initiated radical polymerization to generate phase-separated hydrogels with distinct microdomains. Our bottom-up approach to engineering macromolecular hydrophobicity of conventional hydrophilic, non-phase separating dextrans to induce robust phase transition and generate thermoresponsive phase-separated biomaterials will find applications in mechanobiology, tissue repair and regenerative medicine.

Biomechanical Investigation of Red Cell Sedimentation Using Blood Shear Stress and Blood Flow Image in a Capillary Chip.

Blood image intensity has been used to detect erythrocyte sedimentation rate (ESR). However, it does not give information on the biophysical properties of blood samples under continuous ESR. In this study, to quantify mechanical variations of blood under continuous ESR, blood shear stress and blood image intensity were obtained by analyzing blood flows in the capillary channel. A blood sample is loaded into a driving syringe to demonstrate the proposed method. The blood flow rate is set in a periodic on-off pattern. A blood sample is then supplied into a capillary chip, and microscopic blood images are captured at specific intervals. Blood shear stress is quantified from the interface of the bloodstream in the coflowing channel. τ0 is defined as the maximum shear stress obtained at the first period. Simultaneously, ESRτ is then obtained by analyzing temporal variations of blood shear stress for every on period. AII is evaluated by analyzing the temporal variation of blood image intensity for every off period. According to the experimental results, a shorter period of T = 4 min and no air cavity contributes to the high sensitivity of the two indices (ESRτ and AII). The τ0 exhibits substantial differences with respect to hematocrits (i.e., 30-50%) as well as diluents. The ESRτ and AII showed a reciprocal relationship with each other. Three suggested properties represented substantial differences for suspended blood samples (i.e., hardened red blood cells, different concentrations of dextran solution, and fibrinogen). In conclusion, the present method can detect variations in blood samples under continuous ESR effectively.

Size separation of sodium dodecyl sulfate-proteins by capillary electrophoresis in dilute and ultra-dilute dextran solutions.

SDS capillary gel electrophoresis is a widely used in the biopharma and the biomedical fields for rapid size separation of proteins. However, very limited information is available on the use of dilute and ultra-dilute sieving matrices for SDS-protein analysis. Here, background electrolytes (BGEs) containing 1%-0% dextran were used in borate-based BGE to separate a protein sizing ladder (PSL)≤225kDa and the intact and subunit forms of a therapeutic monoclonal antibody (mAb). The separation performance for the PSL and mAb components differed significantly with decreasing dextran concentration. Ferguson and reptation plots were used to elucidate the separation mechanism. Highly diluted dextran solutions resulted in linear Ferguson plots for both solute types (cf. Ogston theory) in spite of this model assumes a rigid pore structure, thus cannot describe the separation mechanism in ultra-dilute polymer solutions with no reticulations. The saddle differences between the resolution of the PSL and the intact/subunit mAb forms in ultra-dilute dextran-borate matrices suggested the importance of shape selectivity, manifested by the adequate separation of the SDS covered intact as well as light and heavy chain subunits of the therapeutic mAb even at zero dextran concentration.

Characterization of Some Dermato-Cosmetic Preparations with Marine Lipids from Black Sea Wild Stingray.

The traditional knowledge about the therapeutic and nutritional value of fish has been unanimously recognized among the population since ancient times. So, thanks to the therapeutic virtues of these marine animals, it was possible to develop therapies for certain pathologies as well as the use of bioactive compounds as adjunctive therapies incorporated into the treatment regimen of patients. In the present study, stingray liver oil from wild species collected from the Romanian coast of the Black Sea was isolated and analyzed. Fatty acid analysis was performed by gas chromatography. The analysis of the distribution of fatty acids in the composition of stingray liver oil indicates a ratio of 2.83 of omega 3 fatty acids to omega 6, a ratio of 1.33 of polyunsaturated fatty acids to monounsaturated fatty acids, an iodine index of 111.85, and a total percentage of 68.98% of unsaturated fatty acids. Stingray liver oil was used to evaluate the healing action after preparing a fatty ointment. According to the experimental data, a complete regeneration capacity of the wounds was noted in 12 days without visible signs. Four emulgels with stingray liver oil were formulated and analyzed from a rheological and structural point of view in order to select the optimal composition, after which the anti-inflammatory effect on inflammation caused in laboratory rats was studied and an anti-inflammatory effect was found significant (a maximum inhibitory effect of 66.47% on the edemas induced by the 10% kaolin suspension and 65.64% on the edemas induced by the 6% dextran solution).

Dextran-Coated Iron Oxide Nanoparticles Loaded with 5-Fluorouracil for Drug-Delivery Applications.

This study aims to design and test different formulations composed of dextran-coated iron oxide nanoparticles (IONPs) loaded with 5-Fluorouracil (5-FU) with varying nanoparticle:drug ratios on colorectal cancer cells. The stable suspension of IONPs s was synthesized by the adapted co-precipitation method. The stable suspension of IONPs was mixed with a solution of dextran and 5-FU solubilized in a saline solution. The final suspensions with optimized ratios of IONP:5-FU in the final suspension were 0.5:1, 1:1, and 1.5:1. The information on the morphology and size distribution of the IONPs suspension and IONP loads with 5-FU was obtained using scanning electron microscopy (SEM). The presence of 5-FU and dextran on the surface of the IONPs was highlighted by energy-dispersive X-ray spectroscopy (EDS) studies. The determination of the surface charge of the nanoparticles in the final suspensions of IONP:5-FU was achieved by measuring the zeta potential (ζ). The hydrodynamic diameter of the resulting suspensions of IONP:5-FU was determined by dynamic light scattering (DLS). A cytocompatibility analysis was performed using Caco-2 (human epithelial colorectal adenocarcinoma) cells. In this research, our goal was to find a relationship between the formulation ratio of nanoparticles and drug, and the cellular response after exposure, as a strategy to increase the efficacy of this drug-delivery system. The nanoparticle uptake and antitumor activity, including modulation of oxidative stress, apoptosis, and proliferation biomarkers, were analyzed. The present study showed that the nanoformulation with the ratio IONP:5-FU 1.5:1 had the highest anti-tumor efficiency. Moreover, decreased MCM-2 expression in Caco-2 cells exposed to dextran-coated iron oxide nanoparticles loaded with 5-FU was demonstrated for the first time.

STUDY OF BLOOD SEDIMENTATION UNDER A DILUTION OF DEXTRAN SOLUTIONS. BLOOD AS ACTIVE COLLOID SYSTEM

The dynamics of sedimentation of whole blood cells, as well as erythrocyte mass, has been studied during sequential dilution with dextran solutions in vitro. Nonmonotonic changes in the optical density of the boundary between erythrocytes sedimenting both in their own plasma and in model systems were revealed. The oscillations of both the average rate and the instantaneous rates of the process recorded at the cell sedimentation boundary confirm the non-linear and cooperative nature of erythrocyte sedimentation, which is associated with the formation and separation of phases between the cellular, liquid and gaseous components of blood. The structural rearrangement of this dynamic system can be modulated by various factors, for example, dextran solutions used in clinical practice for blood loss, for detoxification, treatment and prevention of thrombophlebitis and shock conditions. It was shown that both the degree of dilution of blood or model systems with dextrans and their molecular weight change the macro- and microkinetics of the process of erythrocyte sedimentation. These results are consistent with the phase hypothesis of sedimentation of blood cells as a biocolloid, and can also be used in clinical blood transfusion for selecting an individual dosage of infusion solutions.

Calibrating and Validating the MFI-UF Method to Measure Particulate Fouling in Reverse Osmosis.

This study aimed to calibrate and validate the MFI-UF method in order to ensure the accuracy of particulate fouling measurements in RO. Firstly, the MFI-UF calibration was examined using two solutions of standard particles (dextran and polystyrene). Two main criteria were investigated: (i) MFI-UF linearity with particle concentrations at both low and high ranges of fouling potential and (ii) the reproducibility of MFI-UF linearity. Dextran solutions showed a strong MFI-UF linearity over the entire range of measured MFI-UF. However, the linearity was not reproducible, and different batches of dextran prepared under the same conditions produced very variable results. For polystyrene solutions, the MFI-UF linearity was verified at the higher range of MFI-UF (>10,000 s/L2), while the MFI-UF at the lower range (<5000 s/L2) appeared to be underestimated. Secondly, MFI-UF linearity was investigated using natural (surface) water under a wide range of testing conditions (at 20-200 L/m2·h using 5-100 kDa membranes). Strong MFI-UF linearity was obtained over the entire range of measured MFI-UF (up to 70,000 s/L2). Thus, the MFI-UF method was validated to measure different levels of particulate fouling in RO. However, future research focusing on MFI-UF calibration is still required through the selection, preparation, and testing of heterogeneous mixtures of standard particles.

Anionic polysaccharides for stabilization and sustained release of antimicrobial peptides

Chemical and enzymatic in vivo degradation of antimicrobial peptides represents a major challenge for their therapeutic use to treat bacterial infections. In this work, anionic polysaccharides were investigated for their ability to increase the chemical stability and achieve sustained release of such peptides. The investigated formulations comprised a combination of antimicrobial peptides (vancomycin (VAN) and daptomycin (DAP)) and anionic polysaccharides (xanthan gum (XA), hyaluronic acid (HA), propylene glycol alginate (PGA) and alginic acid (ALG)). VAN dissolved in buffer of pH 7.4 and incubated at 37 °C showed first order degradation kinetics with a reaction rate constant kobs of 5.5 × 10-2 day−1 corresponding with a half-life of 13.9 days. However, once VAN was present in a XA, HA or PGA-based hydrogel, kobs decreased to (2.1–2.3) × 10-2 day−1 while kobs was not affected in an alginate hydrogel and a dextran solution (5.4 × 10-2 and 4.4 × 10-2 day−1). Under the same conditions, XA and PGA also effectively decreased kobs for DAP (5.6 × 10-2 day−1), whereas ALG had no effect and HA even increased the degradation rate. These results demonstrate that the investigated polysaccharides (except ALG for both peptides and HA for DAP) slowed down the degradation of VAN and DAP. DSC analysis was used to investigate on polysaccharide ability to bind water molecules. Rheological analysis highlighted that the polysaccharides containing VAN displayed an increase in G’ of their formulations, pointing that the peptides interaction act as crosslinker of the polymer chains. The obtained results suggest that the mechanism of stabilization of VAN and DAP against hydrolytic degradation is conferred by electrostatic interactions between the ionizable amine groups of the drugs and the anionic carboxylate groups of the polysaccharides. This, in turn, results in a close proximity of the drugs to the polysaccharide chain, where the water molecules have a lower mobility and, therefore, a lower thermodynamic activity.

Single Hydrogel Particle Mechanics and Dynamics Studied by Combining Capillary Micromechanics with Osmotic Compression.

Hydrogels can exhibit a remarkably complex response to external stimuli and show rich mechanical behavior. Previous studies of the mechanics of hydrogel particles have generally focused on their static, rather than dynamic, response, as traditional methods for measuring single particle response at the microscopic scale cannot readily measure time-dependent mechanics. Here, we study both the static and the time-dependent response of a single batch of polyacrylamide (PAAm) particles by combining direct contact forces, applied by using Capillary Micromechanics, a method where particles are deformed in a tapered capillary, and osmotic forces are applied by a high molecular weight dextran solution. We found higher values of the static compressive and shear elastic moduli for particles exposed to dextran, as compared to water (KDex≈63 kPa vs. Kwater≈36 kPa, and GDex≈16 kPa vs. Gwater≈7 kPa), which we accounted for, theoretically, as being the result of the increased internal polymer concentration. For the dynamic response, we observed surprising behavior, not readily explained by poroelastic theories. The particles exposed to dextran solutions deformed more slowly under applied external forces than did those suspended in water (τDex≈90 s vs. τwater≈15 s). The theoretical expectation was the opposite. However, we could account for this behaviour by considering the diffusion of dextran molecules in the surrounding solution, which we found to dominate the compression dynamics of our hydrogel particles suspended in dextran solutions.

Biomechanical Assessment of Red Blood Cells in Pulsatile Blood Flows

As rheological properties are substantially influenced by red blood cells (RBCs) and plasma, the separation of their individual contributions in blood is essential. The estimation of multiple rheological factors is a critical issue for effective early detection of diseases. In this study, three rheological properties (i.e., viscoelasticity, RBC aggregation, and blood junction pressure) are measured by analyzing the blood velocity and image intensity in a microfluidic device. Using a single syringe pump, the blood flow rate sets to a pulsatile flow pattern (Qb[t] = 1 + 0.5 sin(2πt/240) mL/h). Based on the discrete fluidic circuit model, the analytical formula of the time constant (λb) as viscoelasticity is derived and obtained at specific time intervals by analyzing the pulsatile blood velocity. To obtain RBC aggregation by reducing blood velocity substantially, an air compliance unit (ACU) is used to connect polyethylene tubing (i.d. = 250 µm, length = 150 mm) to the blood channel in parallel. The RBC aggregation index (AI) is obtained by analyzing the microscopic image intensity. The blood junction pressure (β) is obtained by integrating the blood velocity within the ACU. As a demonstration, the present method is then applied to detect either RBC-aggregated blood with different concentrations of dextran solution or hardened blood with thermally shocked RBCs. Thus, it can be concluded that the present method has the ability to consistently detect differences in diluent or RBCs in terms of three rheological properties.