Requirements uncertainty and conflict are the two pervasive phenomena that are currently receiving more attention in the development of information system (IS) projects. The existing literature based on requirements engineering indicates that, as a result of frequent interactions between users and IS practitioners, requirements uncertainty can negatively affect team performance. However, the nature of conflicts and requirements uncertainty have limited exploratory power with regard to software team effectiveness. The main objective of this work is to obtain additional insight into this phenomena by examining the relationships between requirements uncertainty (which comprises both requirements instability and variability) and conflict (i.e. task and relationship conflict). Furthermore, the impact of task and relationship conflict on team effectiveness has also been investigated separately. In order to address these relationships, a theoretical research model was developed and validated through an empirical study based on an online questionnaire survey. We gathered 71 complete responses, mostly from experts within the Indian software industry. The findings of this study indicate that there is a strong positive relationship between requirements instability and variability and relationship conflict; however, task conflict task conflict was not found to have a significant relationship with these dimensions. Additionally, we have observed a strong stronger negative effect of relationship conflict on team effectiveness than that of task conflict during the requirements uncertainty.