Development Of Ryegrass Research Articles

Remediation of soil contaminated with tris-(1-chloro-2-propyl) phosphate using plant and microorganism combined technology: A greenhouse experiment

The remediation of tris-(1-chloro-2-propyl) phosphate (TCIPP) -contaminated soil by the plant (ryegrass, Lolium perenne L.) and microorganism (TCIPP degrading bacteria, Ochrobactrum sp. DT-6) alone or in combination was investigated in this study. TCIPP can inhibit the growth and development of ryegrass and there is a clear dose-effect relationship. Inoculation with strain DT-6 was able to mitigate the toxic influence of TCIPP on ryegrass, but this mitigation effect was not significant. TCIPP in the soil was relatively easy to be uptaken by the ryegrass roots and migrated to the shoots. Furthermore, as the soil TCIPP concentration rose, the concentration of TCIPP in ryegrass also exhibited a corresponding increase. The biological concentration factor (BCF) ranged from 0.33 to 1.88 and the biological accumulation coefficient (BAC) ranged from 0.54 to 3.98. They all significantly decreased with higher soil TCIPP concentrations. The translocation factor (TF) values ranged from 1.55 to 2.34. Inoculation of strain DT-6 significantly reduced TCIPP concentrations in ryegrass roots, stems, and leaves as well as the values of BAC and BCF under low and medium TCIPP concentration treatment conditions, but the effect on TF values was not remarkable. The planting of ryegrass significantly raised the elimination of TCIPP from the soil to 64.6–93.3%, but the influence of inoculation with strain DT-6 on the remediation effect by ryegrass was not significant. The percentage contribution of phytoextraction to the elimination of TCIPP from soils ranged from only 0.64–5.23%.

Nitrogen Application Alleviates the Adverse Effects of Defoliation Stress on Lolium perenne L. by Enhancing the Antioxidant System and Promoting Photosynthesis

Perennial grasses undergo compensatory growth after defoliation. Nitrate is the main nitrogen source for the growth of perennial ryegrass and plays a significant role in plant resistance to stress. The aim of the study was to understand the physiological mechanism of ryegrass in response to defoliation stress under different nitrate supplies and to explore possible ways to alleviate defoliation stress. We performed pot experiments where 12-week-old ryegrass plants grown in low (0.05 mM KNO3) or moderate nitrate (5 mM KNO3) conditions were defoliated and subsequently supplied with different concentrations of nitrate following defoliation treatments. During the regrowth stage, the regrowth rate, biomass, photosynthetic parameters, and the response of the antioxidant system to low or moderate nitrate supply of ryegrass were investigated. The results showed that moderate nitrate supply after defoliation increased the content of photosynthetic pigments in ryegrass and improved its photosynthetic efficiency. In addition, adding moderate nitrate after defoliation increased the activity of antioxidant enzymes and the accumulation of osmotic regulating substances, thereby enhancing plant resistance, effectively reducing the damage to plants caused by defoliation stress, and promoting plant regrowth, especially for plants grown in a low nitrate environment before defoliation. Therefore, this study showed that the addition of exogenous nitrate could counteract some of the adverse effects of defoliation stress on the growth and development of ryegrass.

Electrokinetic – Enhanced ryegrass cultures in soils polluted with organic and inorganic compounds

The effect of electric fields on seed germination and development of ryegrass (Lolium perenne L.) was studied in clean and contaminated soil with heavy metals and/or PAHs. The application of 0.2 DCV/cm in clean soil near ryegrass seeds enhanced the germination by 75%. The presence of contaminants in soil hindered the germination and growing of ryegrass. However, the application of DC electric field favored the germination and growing of plants compensating the negative effects of the contaminants. The electrode material in anodes has a decisive influence in the germination and growing of ryegrass. Stable anode materials have to be used to avoid the release of toxic ions in the soil that affect the development of the plant. Graphite anodes are very appropriate because they are inexpensive and does not generate toxic effects on plants. The electro-phytoremediation of mixed contaminated soil with ryegrass showed very promising results, especially AC electric fields. The tests with AC current showed the highest biomass production in a treatment of 1 month. The more biomass production the more removal of heavy metals and PAHs from soil.

Desenvolvimento e qualidade do azevém no sub-bosque de angico-vermelho em sistema silvipastoril

The aim of this study was to evaluate the development and quality of ryegrass in an understorey of angico-vermelho in a silvopastoral system. The experimental design was a complete randomized block, with a 2x6 factorial, being two systems of production (full sunlight and silvopastoral) and six times of harvest (15, 30, 45, 60, 75 and 90 days after ryegrass emergence), with three replications. The interception of the photosynthetically active solar radiation, average air temperature, dry matter, leaf area and protein content were evaluated. According to the analysis of variance, the interaction between production system and time of harvest resulted in significant differences for dry matter and the protein content and leaf area resulted in differences only according to the harvest time. The development of ryegrass is influenced by shading, when grown in this study conditions. The shade provided by angico-vermelho reduces the amount of ryegrass dry matter of ryegrass, but this reduction does not limit the use of the specie to compose a silvopastoral system. The total protein content and leaf area of ryegrass are not influenced by the light restriction.

Effect of Foliar Applications of Humic Acid on Growth, Visual Quality, Nutrients Content and Root Parameters of Perennial Ryegrass (Lolium Perenne L.)

In recent years, applying humic acid (HA) has been common in turfgrass management. A series of experiments were conducted to evaluate the effect of HA on qualitative and quantitative characteristics of “Speedygreen” perennial ryegrass (Lolium perenne L.). Different concentrations of HA (0, 100, 400, and 1000 mg L−1) were applied monthly as foliar application. Results showed that leaf phosphorus (P), potassium (K), and zinc (Zn) content, fresh and dry weight, chlorophyll content, and root fresh weights were not affected by HA. Meanwhile, HA improved the root and shoot development, except for root fresh weight. While just 100 mg L−1 improved height, visual quality, nitrogen (N) content, roots length, and surface of roots, all of HA concentrations were effective on iron content. These results suggest that HA foliar application might be of benefit to enhance some nutrients uptake and root development of ryegrass possibly leading to improved drought resistance.

Measurement of soil salinity using electromagnetic induction in a paddy with a densic pan and shallow water table

Rice (Oryza sativa L.) has become a monoculture in the saline lands of the Ebro Valley, Spain. The studied farm has produced rice since the 1970s; one exception was 1999, which enabled us to map the soil salinity. The farm had lateral salinity variations mirrored by the development of rye grass (Lolium multiflorum Lam.) planted in 1999. Our objective is to prove the value of a non-deterministic method using electromagnetic induction (EMI) to map the salinity of the rootable layer in the unfavorable circumstances of a paddy having shallow saline and quasi-artesian water table underneath a continuous densic layer. From our EMI readings and soil sampling, we draw a map of the electrical conductivity of saturated paste extracts (ECe) of the upper soil layer (0–40 cm), with ECe ranging from 1.6 to 20.8 dS m−1 and a mean of 7.9 dS m−1. A main achievement was the establishment of an easy procedure not requiring either: (i) knowledge regarding the salinity of the water table or the relationships between EMI readings and the deep soil composition; or (ii) a normal distribution of the EMI readings or of the ECe; or (iii) assumptions about the physical dimensions of the EMI readings. Our procedure will allow ECe to be mapped on other similar salt-affected paddies, helping to decide if a paddy can be planted with alternate crops for production, weed control, or soil structure improvement.

Interaction between BSM-contaminated soils and Italian ryegrass

The interaction among the bensulfuron-methyl, growth of Italian ryegrass, and soil chemical/biochemical/microbiological parameters was investigated in a microcosm experiment. The bensulfuron-methyl added to the soil can be rapidly degraded by certain fungi and actinomycetes present in the original paddy rice soil. The growth of Italian ryegrass significantly accelerated the in-soil degradation of bensulfuron-methyl in its rhizosphere. The uptake of bensulfuron-methyl by ryegrass increased with increasing dosage level of bensulfuron-methyl. However, the phytoextraction of bensulfuron-methyl by ryegrass contributed insignificantly to the total removal of the soil bensulfuron-methyl. Within the dosage range set in this study, the root development of ryegrass was not adversely affected by the presence of the soil bensulfuron-methyl although the fresh biomass of shoot was slightly reduced in the higher dosage treatments. This can be attributed to the adsorption of the added bensulfuron-methyl by soil colloids and consequently the reduction of bensulfuron-methyl level in the soil pore water to a concentration sufficiently lower than the toxic level. The growth of ryegrass significantly increased soil pH and the activities of phosphatase and peroxidase but reduced the EC and the activities of urease in the rhizospheric soil.

Potencial alelopático de extratos aquosos foliares de aveia sobre azevém e amendoim-bravo

O trabalho teve por objetivo avaliar o efeito alelopático de extratos aquosos de cinco cultivares de aveia branca (Avena sativa L.) e quatro de aveia preta (Avena strigosa Schreb), nas concentrações de 0, 25%, 50% e 100%, sobre a germinação e o desenvolvimento das plântulas de azevém (Lolium multiflorum Lam.) e amendoim-bravo (Euphorbia heterophylla L.). O delineamento utilizado foi o inteiramente casualizado distribuído em um trifatorial (genótipos de aveia x plantas infestantes x concentrações do extrato). Utilizaram-se placas de Petri contendo 25 sementes de plantas infestantes que foram umedecidas com extrato da parte aérea dos genótipos de aveia. As variáveis observadas foram: percentual de germinação, comprimento de radícula e de hipocótilo, avaliados aos 14 dias após a instalação do experimento. Os extratos aquosos das cultivares de aveia branca e preta inibiram o potencial alelopático sobre a germinação e o desenvolvimento das plantas daninhas testadas, e os genótipos de aveia branca 'IPR 126', 'UTF Iguaçu' e 'Fundacep FAPA 43' e de aveia preta 'UPFA 21 Moreninha' e 'UTG 9715' foram mais efetivos na inibição da germinação e no desenvolvimento do azevém e amendoim-bravo.

Monitoring ley pastures and their response to winter cleaning

The purposes of this study were to evaluate subterranean clover-based leys on farms and in experiments using several pasture parameters, and to assess the impact of winter cleaning on the productivity and botanical composition of clover swards. Annual pastures were monitored on a group of 5 farms in the Wagga district and compared with an experimental subterranean clover (Trifolium subterraneum L.) pasture. The major problem in the farm paddocks was a lack of legume biomass due to poor legume densities, a consequence of the use of the soft-seeded cultivar Woogenellup and a high content of grassy weeds. The farmers in the group were unaware of the tools, parameters and benchmarks for making quantitative pasture comparisons. In 2 experiments, a range of subterranean clover swards were generated through the use of cultivars, seeding rate and winter cleaning treatments, grazed at 15 sheep/ha and monitored for 3 years. Appropriate benchmark values for the seed pool of subterranean clover were 300–350 kg/ha in winter and 600–700 kg/ha in summer. On the basis of both winter production, a function of May seedling density (target >1000 seedlings/m2) and spring production, which depended on the cultivar maturity, Junee was superior at Wagga to either Dalkeith (earlier maturing) or Woogenellup (softer seeded). Winter cleaning, using selective herbicides (fluazifop, simazine) to remove grasses and weeds, was advantageous in achieving a high content (>90%) and productivity of subterranean clover, provided that the legume content of the pasture was at least 28%, or >20% of total ground area before herbicide application in winter. In winter-cleaned swards, legume growth increased by up to 80%, legume biomass was improved by up to 46% and legume content increased from <50 to >95%. The main disadvantages of winter cleaning were increased areas of bare ground and reduced total biomass for several weeks after herbicide application, and the rapid development of ryegrass that was resistant to at least 1 of the herbicides used. The strategic use of observations to monitor the performance of pastures and their response to management is discussed.

Forecasting agrobiological properties of wheat straw with different additives—multiple regression models including chemical parameters

Abstract The agrobiological properties of 37 composts prepared from wheat straw with a series of organic or mineral additives have been determined through standard chemical analyses and greenhouse experiments with soils of different carbonate content and in the presence, or absence of mineral fertilization. Plant yield on soils treated with the composts was studied in successive stages of development of rye grass (Lolium rigidum), showing significant differences that paralleled the values of a limited number of compost parameters. Under greenhouse conditions and in the presence of mineral supply, the least matured composts led to improved plant yield only in the early harvests, decreasing thereafter which points to a behavior typical for the microbial immobilization of the additional nutrients. The germination (phytotoxicity) index was found poorly correlated with the yield in the different soils, whereas the N and lignin contents provided the most significant information, the results suggesting no cause‐...

Nitrate and ammonium nutrition in ryegrass: Changes in growth and chemical composition under hydroponic conditions

Abstract Nitrogen (N) is one of the most critical elements affecting grass seed yield. In soil and cropping conditions of Western Oregon, ammonium‐N may play an important role in the growth and development of ryegrass grown for seed. Our objectives were to determine the physiological and biochemical effects of ammonium and nitrate nutrition on ryegrass vegetative growth and subsequent expression of floral tillers, and changes in plant reduced‐N and carbohydrate composition. Plants were grown in hydroponics (active pH‐controlled) and fed nitrate and ammonium alone or in combination at 10 mM total N. The experiment was terminated at anthesis, which occurred at 68 days after planting (DAP). Changes in plant tiller number, height, dry weight, reduced‐N, and water‐soluble carbohydrates (WSC) were recorded. Vegetative growth rate of plants receiving lower ratios of nitrate/ammonium was up to twice those receiving total nitrate or 75/25 nitrate/ammonium. Total primary tiller number at 68 DAP (anthesis) was 30% g...