Development Of Polyherbal Formulation Research Articles

Standardization Quality Control and Development of Poly Herbal Formulation for the Management of Type-2 Diabetes Mellitus

In the Ayurvedic system of medicine, Herbal Drugs For Diabetes Mellitus as mentioned in ancient Indian books like Charak Samhita, Mahdhav Nidan and Astang Sanghra, there are about 600 plants, which are stated to have antidiabetic property Polyherbal antidiabetic formulation consists of six herbs viz., Nigella sativa (seed), Moringa oleifera (seed), Linum usitatissimum (seed), Trogonella foenum (seed), Cinnamum zeylanicum (bark) and Macrotylom auniflorum (seed). Crospovidone, Microcrystalline cellulose, Colloidal Silicon dioxide, PVP Magnesium stearate, (Polyvinyl pyrrolidone). Polyherbal formulation of the extracts of all selected plants was subjected to freeze drying process. The extracts were dried for a period of time according to their rate of drying .Diluents like, Microcrystalline cellulose, Magnesium stearate, Lactose, starch were dried. All active ingredients were weighed according to the formula, mixed with MCC followed by diluents and Glidant like aerosil and magnesium stearate as lubricant as specified in formula were mixed well. The mixture was blended thoroughly for 30minutes. Then the powder was transferred to the polythene bags and labeled for further studies.

Development of Polyherbal Formulation: Impact of Antioxidants on In Vivo Antidepressant Activity in Animal Models

Development of Polyherbal Formulation: Impact of Antioxidants on In Vivo Antidepressant Activity in Animal Models

Development of polyherbal formulation and evaluation of its diuretic activity in rats

An attempt was made in this study to investigate the diuretic activity of an ethanolic extract of a polyherbal formulation containing four drugs: seeds of Coriandrum sativum, buds of Syzigium aromaticum, leaves of Ocimum sanctum, and curcumin (Curcuma longa), as well as leaves of Syzigium aromaticum. According to the findings of this investigation, the extract of a polyherbal formulation (including seeds of Coriandrum sativum, buds and leaves of Syzigium aromaticum & leaves of Ocimum sanctum, and Curcuma longa) possessed substantial activity. Various quantities of Polyherbal formulations (200 and 400 mg/kg), furosemide (10 mg/kg), and vehicle were given orally to rats (n = 6 animals per group), and the urine output was collected after 24 hours. All concentrations of Polyherbal formulation exhibited a dose-dependent relationship when compared to the control animals in the study. According to the findings of this study, the Polyherbal formulations have significant diuretic effect in rats when tested in the above-mentioned experimental model. That polyherbal formulation extract has such potent effect may be due to their ability to stimulate the excretion of Na+, K+, and Cl- concentrations in urine while also increasing the amount of urine excreted by the body. As diuretics, medicinal herbs are a key source of supply. When it comes to diuretics, both mono- and poly-herbal formulations have been employed successfully.

Structural Elucidation of Isolated Phytochemicals from Selected Medicinal Plants with Antihyperlipidemic Activity and Development of Polyherbal Formulation with Densitometric Analysis of Isolated Phytoconstituents

Abstract: Objectives: The present systematic research aimed to express the antihyperlipidemic effects of different types of herbs based on animal studies. The purpose of the present project is to perform the phytochemical investigation and exploration of hypolipidemic activity with the development and quantification of phytoconstituents of polyherbal hypolipidemic formulation employing Apium graveolens, Salvadora persica, Carica papaya and Evolvulus alsinoides. Materials and Methods: Flash chromatography technique was used for the isolation of phytoconstituents from the selected medicinal plants. Antihyperlipidemic study was done on triton induced model. Triton- WR 1339 (400 mg/kg b.w) i.p. injection were given to different animal groups. HPTLC densitometric method was established for the quantification of the phytoconstituents in polyherbal formulation. Results: The phytoconstituents so isolated were identified as 8, 8-Dimethyl-2H, 8H-pyrano [2, 3-f] chromen-2-one, 9- methoxyfuro [3, 2-g] chromen- 7-one, 7- hydroxy-2H-chromen -2-one, Isothiocyanatomethylbenzene and 17-(5-Ethyl- 6-methylheptan-2-yl)-10,13-dimethyl2,3,4,7,8,9,11,12,14,15,16,17-dodecahydro 1Hcyclopenta[α]phenanthren-3-ol through chromatographic separation techniques and their structures were elucidated by spectroscopic analysis. The formulation showed significant reduction in TC, TG, LDL and VLDL level (p<0.01) and significantly raised HDL level (p<0.01) compared to hyperlipidemic group. Furthermore, HPTLC method was established for the quantification of isolated phytoconstituents in polyherbal formulation through densitometric analysis. Conclusion: The present work also characterized active phytoconstituents from different parts of selected plants. The research work involved development of polyherbal hypolipidemic formulation with a view to reduce the serum levels of TC, TG, LDL and to increase the serum level of HDL cholesterol and to establish HPTLC densitometric method for quantification of its phytoconstituents. Key words: Hyperlipidemia, Medicinal Plants, Flash Chromatography, Isolated Phytoconstituents, HPTLC Densitometric Analysis.