Development Of Functional Food Products Research Articles

Physicochemical, Antioxidant, and Sensory Characteristics of Sponge Cake Fortified with Quinoa Flour, Oolong, and White Tea Powder

Nowadays, there is a growing demand for healthy foods enriched with various functional bioactive ingredients. Cakes are ready-to-eat baked products consumed worldwide and are suitable for the development of functional food products. In this study, a Box–Behnken design was used to investigate the effect of three independent variables, including oolong tea powder (OT, 0–20%), white tea powder (WT, 0–15%), and quinoa flour (QF, 0–40%), on the quality characteristics of sponge cake during 21 days of storage. Following the evaluation of the model, the optimum levels of ingredients for the preparation of sponge cake were 15% WT, 17.17% OT, and 24.97% QF. Total phenolic content of 52.09 mg gallic acid/100 g, antioxidant activity of 0.068 mg/mL, overall acceptability of 4.89, lightness of 47.94, and peroxide value of 0.68 mEq/kg were obtained under optimized conditions. The growth of molds and yeasts was prevented during storage time. The hardness, gumminess, and chewiness of the optimized sponge cake improved with increasing WT, OT, and QF levels in comparison to the control. Scanning electron micrographs showed a more porous structure in optimized cake samples. In conclusion, the utilization of OT, WT, and QF as phenolic compounds in sponge cake led to an increase in its nutritional value and improved shelf life.

Bio-fermented milk product based on <i>L. acidophilus</i> fortified by <i>Sanguisorba officinalis</i> extract

The development of new functional food products containing probiotics and prebiotics of plant origin has actual scientific and practical values. The presented study purpose is the creation of fermented milk products based on L.acidophilus with the introduction of an extract of the Sanguisorba officinalis or great burnet as a prebiotic. Extracts from the great burnet rhizomes and roots have an antimicrobial activity, specifically against the bacteria Escherichia coli, Pseudomonas aeruginosa, and Staphylococcus aureus with pronounced bactericidal effect on various microbes of the dysenteric and paratyphoid groups, a detrimental effect on Trichomonas, fungi of the genus Candida and Giardia. Physicochemical and microbiological indicators during ripening and storage of the obtained fermented milk products based on L.acidophilus with great burnet extracts are considered. Titratable acidity, water-holding capacity, the number of lactobacilli, viscosity, and organoleptic values of fermented milk products are analyzed. It is established that the product with great burnet dry extract at a concentration of 10^-3 g/cm^3 has maximum scores for organoleptic, physicochemical, and microbiological indicators. The developed bio-fermented milk product enriched by antioxidants from extracts of biologically active compounds of great burnet will meet the national and international consumers’ demands with recommendations for therapeutic and preventive nutrition and a healthy lifestyle.

Novel Probiotic Candidates in Artisanal Feta-Type Kefalonian Cheese: Unveiling a Still-Undisclosed Biodiversity.

Autochthonous dairy lactic acid bacteria (LAB) isolates encompass a natural source of starter, adjunct, or probiotic candidates. In this context, traditionally manufactured, using exclusively animal rennet, Feta-type cheeses were collected from five farms located in different regions of Kefalonia island (Greece). The primary objective of this study was to isolate and characterize novel LAB, thereby exploring the unmapped microbial communities of Kefalonian Feta-type cheese and identifying new potential probiotics. The initial screening, included a preliminary gastrointestinal (GI) tolerance assessment (acidic conditions and bile salts), followed by their safety evaluation (hemolytic activity and antibiotic susceptibility). Based on the preliminary screening, selected strains underwent molecular identification and were further investigated for their probiotic attributes (lysozyme and phenol resistance, antimicrobial traits, antidiabetic aspects, cholesterol reduction and adhesion, adhesion to Caco-2 cells, and milk acidification potential). The results showed that 49, out of the 93 retrieved isolates, exhibited resistance to GI conditions, whereas 18 met the safety criteria. The molecular identification revealed strains belonging to the species Lactiplantibacillus plantarum, Limosilactobacillus fermentum, Lacticaseibacillus rhamnosus, and Lacticaseibacillus paracasei. The selected rod-shaped 14 isolates displayed a potential probiotic character. The best-performing isolates concerning cholesterol assimilation and adhesion, α-glucosidase inhibition, and epithelial adherence were Lpb. plantarum F89, F162, and F254 and Lcb. paracasei F214 and F216, whereas Lcb. paracasei F70 showed potential as a defined strain starter. The present study explores for the first time the biodiversity of traditionally fermented microbial communities in Kefalonian Feta-type cheese, revealing novel potential probiotic strains that can contribute to the development of innovative functional food products.

Evaluating the Bioactive Compounds of Beetroot and Their Pharmacological Activities in Promoting Health

Purpose: Lifestyle modification and sedentary life have led to multiple comorbidities which necessitate exploitation of underutilized novel ingredients in daily life. This comprehensive review aimed at providing valuable insight into the diverse pharmacological activities of beetroot bioactive compounds and their health enhancing properties. Furthermore, this review explored emerging research by scientific community for utilization of beetroot in the development of novel functional food products. 
 Materials and Methods: The scientific studies focusing on the biological activity and therapeutic potential of beetroot for health parameters were included in this study. Database search was made using google scholar, PubMed and research gate which generated a total of 200 papers. Out of which, only 63 met the inclusion criteria of this study. Inclusion criteria included literature focusing on beetroot’s bioavailability, plant pigments, bioactive compounds, health benefits, as well as studies describing food applications. This review includes studies between 2014 and 2023.
 Findings: The main findings suggested that a prominent bioactive compound and natural betalain pigment in beetroot, exhibits potential anti-inflammatory, anti-carcinogenic and anti-depressive properties. The anti-oxidant activity of betalain is effective against oxidative stress and play a role in preventing and mitigating chronic diseases. Bioactive components including, nitrate content, betanin and betacyanin, potentially modulate blood pressure and promote cardiovascular health. Furthermore, beetroot derived polyphenols, carotenoids, vitamins and minerals contribute to bone health, immune function and overall well-being. Food product development based studies demonstrate an overall improvement in the nutritional and sensory profile. 
 Implications to Theory, Practice and Policy: Further research on bioactive components of multiple varieties of beetroot is recommended to reassess the underlying mechanisms and optimal dosage for unlocking their potential for medicinal, nutraceuticals and dietary interventions. More research is needed by food industrialists and scientists to explore the potential of beetroot pigments as non-synthetic food additives particularly color enhancer in various food formulations.

Nutritional composition, phytochemical extraction, and pharmacological potential of mulberry: A comprehensive review

Mulberry is one of the underutilised fruit with high perishability. The various species of mulberry fruit are characterized by their natural colours as black, white, and red. These fruits are rich sources of the natural pigment anthocyanin, as well as containing substantial quantities of vitamins and minerals. Black mulberry contains a large amount of phytochemicals, particularly flavonoids (anthocyanin) and phenolics (gallic acid, chlorogenic acid, caffeic acid, quercetin, and rutin). These compounds are responsible for its superior pharmacological effects, including antioxidant activity, hyperglycemia, and anti-inflammatory properties. Numerous research were conducted on different extraction techniques to separate bioactive compounds from mulberry. Various components of Morus species also possess potent nutraceutical capabilities, leading to a wide range of clinical trials for identifying its beneficial properties. The present review concentrates on the most recent literature concerning nutritional composition and phytochemical profiling of three distinct species of mulberries, including their fruit, seeds, and leaves. Furthermore, it investigates the process of extracting bioactive compounds from mulberries and investigates their potential uses in pharmacology as well as in the development of functional food or nutraceutical products. This research thoroughly analyzes innovative approaches for utilizing an underutilized perishable crop to generate economically viable functional products.

Beetroot-based blended juice: Process development, physico-chemical analysis and optimization of novel health drink

The demand for the preparation of functional and nutrients-rich juice or beverages for the boosting immunity is the prime focus of consumers. So, this study was aim to optimization of different proportion for the preparation of blended juice from beetroots, grapes, turmeric, and lemons by using response surface methodology (RSM) and assessment of the quality parameters such as acidity, ascorbic acid, antioxidant activity and overall acceptability of blend juice were considered. Beetroot is widely recognized for its pigments known as betalains and have been utilized in the development of functional food and beverage products. Beetroots, grapes, turmeric, and lemons have been used in this study to prepare functional drinks, known for their nutritional value and medicinal properties. To optimize the blend juice, the dependent variables mainly beetroot juice, grapes juice, turmeric juice, and lemon juice in the range of 50–60, 30–40, 1–5, and 1–5 mL, were selected respectively. The optimized formulation for blended juice with a maximum desirability value of 0.921, an acidity of 0.60 %, an ascorbic acid of 14.17 mg/100 mL, an antioxidant activity of 93.94 %, and an overall acceptability of 8.07 was finalized at 53.12 mL of beetroot juice, 40 mL of grapes juice, 1.88 mL of turmeric juice and 5 mL of lemon juice.

Antioxidant activity according to bioactive compounds content in dried pumpkin waste

In this study freeze-drying and oven-drying (at 50 and 65 ?C) were applied for the preparation of dried pumpkin waste. The effect of drying methods on physical properties (moisture content, water activity, hygroscopicity, water solubility, bulk and tapped density, flowability and colour), content of bioactive compounds (carotenoids and phenolics) and antioxidant activity were investigated. Also, the influence of one-year storage at ?20 ?C on the bioactive compounds content and the antioxidant activity of dried pumpkin waste was examined. Results indicated that drying method showed a significant impact on the investigated characteristics. Bioactive compounds content and antioxidant activity by DPPH test of freeze-dried were higher than of oven-dried pumpkin waste, while higher antioxidant activity in reducing power assay was determined by oven-dried pumpkin waste. An artificial neural network model was developed, for the anticipation of antioxidant activity according to bioactive compounds content (phenolics and carotenoids), in oven-dried (at 50 and 65 ?C) and in freeze-dried pumpkin waste after one-year storage. These models showed good prediction properties (the r2 value during training cycle for output variables was 0.999). It was demonstrated that pumpkin waste is potentially an important source of bioactive compounds, which can be used after extraction in suitable forms in the development of functional food products.

Exploring the excellence of commercial Brahmi products from Thai online markets: Unraveling phytochemical contents, antioxidant properties and DNA damage protection

Brahmi (Bacopa monnieri (L.) Wettst.) is extensively used as a nutritional supplement in various commercial products as the plant contains abundant phytochemicals and has antioxidant properties. This study assessed the phytochemical contents, antioxidant properties, and DNA damage protection among seven Brahmi products sold through Thai online markets. Results showed that the P6 sample exhibited 3.5–7.5 fold higher bacoside contents than values observed in the other six product samples. The P6 sample also demonstrated the highest TTC, TFC, and TPC compared to the other brands. For antioxidant activity, the samples (P1–P7) displayed high capacity to scavenge DPPH free radicals with slightly significant differences ranging from 78.37 ± 0.25 to 87.21 ± 0.05 at p-value ≤0.01. The P6 sample showed strong protection against H2O2-induced oxidation of DNA strand breakage, indicating highly potent phytochemical compounds with effective free radical scavenging activity, and the ability to prevent DNA damage. The P6 sample showed promise as a valuable ingredient for the development of functional food products. However, further in vivo animal and clinical studies are required to explore the neuroprotective enhancement effects of Brahmi extracts.

Development of technology for obtaining encapsulated probiotics

One of the priority areas of the modern food industry is the development of functional food products that have a regulatory effect on physiological functions, biochemical reactions and psychosocial behavior of a person through the normalization of his microecological status. Scientists assign a special role in functional nutrition to fermented milk products with probiotics, which have a more pronounced functional effect on the human body, due to the complex action of probiotics. However today traditional production technologies face some problems, in particular, the problem of preserving and delivering viable probiotic cells to the gastrointestinal tract in order to display therapeutic properties. In this regard, the use of methods for encapsulating probiotics to obtain capsules and their application in the technology of fermented milk products for functional purposes is relevant. This article discusses the technology for obtaining encapsulated probiotics. To obtain encapsulated probiotics, several types of biopolymers were used: amidated pectin, sodium alginate, and gelatin. To substantiate the selected encapsulating materials, experimental studies were carried out to determine the possibility of capsule formation of amidated pectin, sodium alginate and gelatin at a concentration of 1 %, 2 %, 3 %, as well as the ratio of gelatin and sodium alginate 1/1 %, 2/1 %, 3/1 %, 4/1 %.

Ultrasonic extraction of Moringa oleifera seeds polysaccharides: Optimization, purification, and anti-inflammatory activities

Natural polysaccharides exhibit numerous beneficial properties, such as antioxidant, antitumor, hypoglycemic, and hypolipidemic activities. Moringa oleifera seeds are of high dietary and therapeutic value which drew a lot of attention. However, the regulation effect on anti-inflammatory activity of polysaccharides remains to be studied. Herein, novel bioactive polysaccharides (MOSP-1) were extracted from Moringa oleifera seeds, and the anti-inflammatory properties of MOSP-1 were uncovered. Ultrasound-assisted extraction (UAE) was used to prepare the polysaccharides with optimized conditions (70 °C, 43 min, and liquid-solid-ratio 15 mL/g). Then, DEAE-Sepharose Fast Flow columns were applied to isolate and purify MOSP-1. Rhamnose, arabinose, galactose, and glucose were identified as the monosaccharide constituents of MOSP-1, with a molecular weight of 5.697 kDa. Their proportion in molarity was 1:0.183:0.108:0.860 and 8 types of glycosidic linkages were discovered. Bioactive assays showed that MOSP-1 possessed scavenging activities against DPPH and ABTS radicals, confirming its potential antioxidation efficacy. In vitro experiments revealed that MOSP-1 could reduce the expression of inflammation-related cytokines, inhibit the activation of ERK, JNK, and p38 (the MAPK signaling pathway), and enhance phagocytic functions. This study indicates that polysaccharides (MOSP-1) from Moringa oleifera seeds with anti-inflammatory properties may be used for functional food and pharmaceutical product development.

Characterization of microencapsulated spinach extract obtained by spray-drying and freeze-drying techniques and its use as a source of chlorophyll in a chewing gum based on Pistacia atlantica

This study aimed to extract and encapsulate spinach extract as a source of chlorophyll (CHL) through freeze- (FD) and spray-dried techniques (SD), followed by utilizing microencapsulated CHL (mCHL) in development a functional chewing gum (CG) based on Pistacia atlantica. The characteristics of mCHL underwent significant alterations through the employment of various concentrations (25 and 35% w/w) and amalgamations of Arabic gum (0–8.75 g) and maltodextrin (18.75–35 g). The mean values for powder yield and encapsulation efficiency in FD powders at 25% total solid content (TS) were 19.24% and 5.28% greater, respectively, than those of spray-dried samples, while SD microcapsules exhibited significantly enhanced storage stability. Microscopic analysis revealed the considerable impact of drying method, carrier type, concentration, and ratio on both the size and surface properties of FD- and SD-mCHL. An increase in TS from 25% to 35% resulted in a significant increase in mean particle size for SD powders from 7.19 μm to 10.96 μm. The textural analysis of CG demonstrated that the incorporation of CHL, in both free and encapsulated forms, did not negatively affect its hardness, adhesiveness, springiness, cohesiveness, chewiness, and resilience. Furthermore, the sensorial attributes of the CG samples, including appearance, spinach taste, and overall acceptability, were improved. Oral release analysis showed that after 15 min of chewing, the CHL release from CG samples was 94.5% and 70.3%, respectively, indicating that SD provided a slow release mechanism for CHL. In conclusion, both FD and SD CHL exhibited favorable technological traits and can be utilized as a delivery vehicle towards development of functional food products.

Nutritional and Sensory Evaluation of Yoghurt Incorporated with Unripe False Horn Plantain (Musa paradisiaca var. "apentu").

Unripe plantain (Musa paradisiaca L.) is rich in nutrients including minerals, vitamin C, and carbohydrates particularly resistant starches with prebiotic properties. However, the fruit is challenged with limited utilisation, and this contributes to its high postharvest losses along the production and supply chain. Information is lacking on incorporating plantain (UPF) in functional dairy food product development. In a completely randomized design, the study evaluated the effect of unripe false horn plantain (var. "apentu") flour (UPF) incorporation (w/v), at 0% (control), 2%, 4%, and 6%, on the composition and sensory quality of yoghurt. The results showed that higher UPF percent incorporation resulted in yoghurts having lower moisture and higher total solid values as well as enhanced nutritional values, in terms of protein, zinc, potassium, calcium, and vitamin C (P < 0.05). Mean pH and total titratable acidity values of the yoghurt products were in the ranges of 3.40-3.65 and 1.00-130%, respectively. Conversely, an increase in UPF incorporation generally reduced consumer likeness scores for yoghurt sensory characteristics including appearance, texture, flavour, taste, aftertaste, and overall acceptability. The control AZ product received the highest ratings in all sensory attributes evaluated. Compared with the control AZ, the BX (2% w/w UPF) yoghurt showed better nutritional quality as well as had comparable ratings for the sensory attributes, particularly in terms of appearance, texture, and flavour. Thus, the formulation containing 2% UPF has the best potential for the production of value-added functional yoghurt, which will be acceptable. However, for high acceptability, further research is needed to improve the impact of UPF incorporation on the overall sensory quality of yoghurt. The study suggests that UPF can serve as a potential supplement for improving the value of yoghurt, and this also contributes to reducing postharvest losses of plantain as a key food security resource. Also, the study findings contribute baseline information to guide future research on functional dairy product development with unripe plantain.

Technology of functional dessert dishes with ancillary products

The article considers the possibility of increasing the biological value of dessert dishes thanks to the use of fruit powders and apiproducts. There is a shortage of minerals, vitamins and other minor nutrients, dietary fibers in the diets of the population of Ukraine against the background of consumption of excessive amounts of sucrose and animal fats. One of the ways to solve this problem is the development of functional food products, in particular functional dessert dishes using dietary supplements and plant raw materials. Promising raw materials for use in dessert dishes are fruit powder and apiproducts, which contain a significant amount of vitamins, minerals, and dietary fibers. The expediency of their use in order to improve the nutritional value and increase the biological value of dessert dishes has been proven. It is advisable to introduce desserts into the diet as healthy, therapeutic and preventive dishes. Based on the results of the research, the technology of functional desserts with an improved content of essential nutrients was developed. Based on the obtained data, it can be concluded that the developed desserts enrich the human diet with deficient nutrients and reduce the amount of easily digestible carbohydrates. Developed desserts can be recommended in diets to prevent diabetes, to improve digestion and strengthen immunity. The social effectiveness of the developed dessert technology consists in expanding the range of dessert dishes with a reduced amount of easily digestible carbohydrates. The developed dessert products can be recommended for children's nutrition, in the daily diets of people working in heavy industry, living in ecologically polluted areas and all strata of the population, to meet consumer demand for functional food products, as well as for people with diabetes and will contribute to improving the quality of nutrition and improving the health of the population of Ukraine. Developed desserts can be used in restaurants.

Evaluation of the Efficacy of Kombucha-Based Drink Enriched with Inulin and Vitamins for the Management of Constipation-Predominant Irritable Bowel Syndrome in Females: A Randomized Pilot Study

BackgroundConstipation-predominant irritable bowel syndrome (IBS-C) mainly affects females, and dietary interventions for symptom relief often yield poor results because of low patient adherence. The development of functional food products enriched with dietary fibers may increase patients’ adherence to a healthy diet and relieve IBS-С symptoms. ObjectiveThis proof-of-concept, open-label, randomized controlled pilot study is aimed to evaluate the efficacy of kombucha enriched with inulin and vitamins in females with IBS-C. MethodsForty females with IBS-C were randomly assigned to receive either 220 mL of kombucha enriched with inulin (2.53 g/220 mL) and vitamins (B1 – 0.59 mg, B2 – 0.55 mg, B3 – 5.9 mg, B6 – 0.7 mg, and folic acid – 81.4 μg/220 mL) or water for 10 d. Stool frequency, Bristol stool scale score (BSSS), and abdominal symptoms were evaluated using a 5-point Likert scale on days 5, 9 and 14 of the study. The palatability of the drink was assessed using a visual analog scale. ResultsAfter 10 d, the kombucha group showed a significant increase in stool frequency (0.60 ± 0.31–0.85 ± 0.19 times/d; P = 0.004) compared with the control (0.63 ± 0.33 compared with 0.72 ± 0.28; P = 0.6). The mean values of the BSSS increased in the kombucha group (3.0 ± 1.2–4.4 ± 1.0; P = 0.001), whereas they remained unchanged in the control (2.9 ± 1.2 compared with 3.4 ± 1.2; P = 0.6). The kombucha group also experienced a significant decrease in the feeling of incomplete bowel emptying (1.88 ± 0.78 compared with 1.41 ± 0.56 points; P = 0.015), which was not observed in the control group. ConclusionsShort-term consumption of kombucha enriched with inulin and vitamins was associated with an increase in stool frequency, an improvement in the BSSS, and a reduction in the feeling of incomplete bowel emptying in females with IBS-C. Further large-scale clinical trials investigating the efficacy of kombucha enriched with inulin and vitamins in patients with IBS-C are warranted to prove the observed effects. Trial registration numberThis trial was registered at clinicaltrials.gov as NCT05164861 (==https://clinicaltrials.gov/study/NCT05164861?term=NCT05164861&rank=1; registered on 18 December, 2021).

Nutritional and therapeutic potential of functional components of brown seaweed: A review

Seaweed has a unique chemical composition with an abundance of bioactive substances. In Russia, brown seaweed grows in the coastal areas of the Pacific Ocean (Far East) and the seas of the Arctic Ocean.&#x0D; This review focuses on the therapeutic and nutritional potential of functional components of brown seaweed. It was based on a systematic analysis of research and review articles published from 2010 to 2023 and indexed in Scopus, Web of Science, and eLIBRARY.RU. Our particular interest was in seaweed’s bioactive components such as polysaccharides, phenolic compounds, vitamins, lipids and fatty acids, proteins, peptides, and amino acids.&#x0D; Compounds extracted from brown seaweed exhibit antioxidant, antiglycemic, antitumoral, neuroprotective, anti-inflammatory, anticoagulant, antibacterial, and immunostimulating properties. Brown seaweed and its derivatives are used as structural modifiers, antioxidants, preservatives, moisture-retaining agents, and sources of vitamins and minerals in the development of functional and preventive food products. They are also used as ingredients in meat, dairy, bakery and flour products, as well as in food additives and beverages, to provide potential health benefits and essential nutrients.&#x0D; Studies have proven the functional effectiveness of food products containing brown seaweed and its derivatives. The incorporation of seaweed components into functional foods could contribute to global food security. More research is needed to develop new competitive products based on seaweed and to investigate them for the presence of substances hazardous to humans and the environment.

Functional Food Product Development and Quality Analysis of Whey Based Fruit Herbal Beverage

This study focuses on development of herbal fruit beverage based on whey. Whey was mixed with pineapple juices along with mentha flavor in the ratio 8:2:1. This study evaluated the physicochemical, sensory and microbial analysis of the developed product periodically till a period of 20 days. The analyzed physico-chemical parameters in the product reported a decline from 0.02 to 2% as the storage period increased. 0.18% of fat reduced to 0.16% during the study period. Protein content reduced up to 0.13%. Lactose content was reduced to 0.2% at the end of 20 days. Sensory evaluation had a high reading of 8.32 for taste and a least value of 6.64. The hedonic scale reading drastically decreased after ten days of storage. Whey based pineapple beverage can serve as a best alternative functional food in the present-day scenario.

Dihydrocaffeic acid grafted chitosan self-assembled nanomicelles with enhanced intestinal transport and antioxidant properties of chicoric acid.

Chicoric acid (CA) plays a crucial role as a functional factor within the realm of foods, showcasing a wide array of bioactivities. Nevertheless, its oral bioavailability is significantly limited. To optimize the intestinal absorption and bolster the antioxidant capacity of CA, a water-soluble dihydrocaffeic acid grafted chitosan copolymer (DA-g-CS) was synthesized using a conventional free radicals system, and subsequently utilized for the encapsulation of CA within self-assembled nanomicelles (DA-g-CS/CA). The average particle size of DA-g-CS/CA was 203.3nm, while the critical micelle concentration was 3.98×10-4 mg/mL. Intestinal transport studies revealed that DA-g-CS/CA penetrated cells via the macropinocytosis pathway, exhibiting the cellular uptake rate 1.64 times higher than that of CA. This substantial enhancement in the intestinal transport of CA underscores the significant improvements achieved through DA-g-CS/CA delivery. The pharmacokinetic results demonstrated that DA-g-CS/CA exhibited a remarkable bioavailability 2.24 times that of CA. Furthermore, the antioxidant assessment demonstrated that DA-g-CS/CA exhibited exceptional antioxidant properties in comparison to CA. It demonstrated enhanced protective and mitigating effects in the H2O2-induced oxidative damage model, while also displaying a stronger emphasis on protective effects rather than attenuating effects. These findings aim to establish a solid theoretical foundation for the advancement of CA in terms of its oral absorption and the development of functional food products.

Evaluation of jamu kunyit asam (Curcuma domestica Val. - Tamarindus indica L.) as probiotic carrier of Lactobacillus plantarum BP102

Jamu kunyit asam is a herbal health product with the main ingredients of turmeric (Curcuma domestica) and tamarind (Tamarindus indica). The addition of probiotics in jamu kunyit asam will contribute to better-quality fermented drink. The purposes of the present work were (1) to determine the viability and probiotic potential of Lactobacillus plantarum BP102 in jamu kunyit asam, (2) to determine the effect of the addition of L. plantarum BP102 on the antioxidant activity of jamu kunyit asam, and (3) to determine the organoleptic changes of fermented jamu kunyit asam. The probiotic strain L. plantarum BP102 was able to grow in jamu kunyit asam with a density of 107 - 108 CFU/mL, followed by a decrease in pH value after 5-d storage. The L. plantarum BP102 in jamu kunyit asam also showed its character as a probiotic, namely, survival rates at low pH and in the presence of bile salts of 63 - 70 and 73 - 83%. The antioxidant activity of fermented jamu kunyit asam did not show an increase. The addition of L. plantarum BP102 was able to improve the organoleptic quality of fermented jamu kunyit asam, especially in terms of flavour and colour. Therefore, jamu kunyit asam can act as a probiotic carrier of L. plantarum BP102 for the development of fermentation-based functional food products.

Factors Affecting the Starch Extraction from Jackfruit Seed (Artocarpus heterophyllus L.)

The study was conducted to investigate some factors affecting the starch extraction from jackfruit seeds (JS), including: Jackfruit seed drying temperature, extraction time and ratio of water to jackfruit seed flour (JSF). The JS were dried at various temperatures (50, 60 and 70oC) before grinding into flour. Starch was extracted in 4 levels of time (5, 6, 7 and 8 hours) interacted with 4 ratios of water to JSF (10, 15, 20 and 25 times, v/w). The efficiency of the extraction process was evaluated through the yield and the physicochemical properties of JSS, such as starch content, the color (L value and browning index - BI), water absorption index (WAI), water solubility index (WSI) and swelling power (SP); the scanning electron microscopy (SEM) and the average particle diameter (APD). The drying temperature of 60oC is considered suitable for obtaining good flour for performing starch extraction. Starch extraction from the JSF was carried out with the ratio of water to JSF of 15 times (v/w) for 6 hours at ambient temperature. The yield was 44.96?0.29%; the starch content was 92.06?0.48%. The JSS exhibited the values of WAI, WSI and SP in range of from other starches. The APD of JSS is 6.90–8.50 µm and is classified as small granules. All physicochemical properties as well as the APD of JSS were interrelated. These results showed that the extraction is high yield. The extracted JSS has high starch content and suitable physicochemical properties. When purified effectively, it has the potential to become food or effective complementary ingredients in the development of functional food products.

Enzymes of microorganisms. Proteases of lactic acid bacterias

The work is devoted to the enzymes of microorganisms. This review article presents the characteristics of microbial enzymes, their classifications according to various parameters. The study examines the role of microbial enzymes in various areas of human life. Enzymes are involved in the biochemical processes of microorganisms for their protection, reproduction, and growth. Enzyme producers are various taxonomic groups of bacteria, filamentous fungi, actinomycetes and yeasts. The article describes hydrolytic enzymes, reveals the importance of the protease enzyme involved in various processes with microorganisms. The main producers of proteolytic enzymes are bacteria of the genus Bacillus, Lactobacillus, Streptococcus, Micrococcus, Pseudomonas, fungi of the genus Aspergillus, Penicillium, Blakeslea, actynomicetes of the genus Streptomyces, Actinomyces. Particular attention is paid to lactic acid bacteria. The significance of the use of protease in the biotechnological industry, in particular, for the development of preparations, starter cultures, bioadditives, and functional food products with desired properties, is shown. The article mentions the results of a study of the proteolytic activity of cultures of microorganisms, obtained by me and my colleagues. The objects of study were bacilli, yeast, fungi and lactic acid bacteria.To assess proteolytic activity, in vitro methods, chromatographic and other methods are used.