Development Of Chassis Research Articles

Advances in artificial cells based on microfluidic chips

One of the main goals of synthetic biology is to build artificial cells in a bottom-up manner, which not only facilitates the deep understanding of the origin of life and cell function but also plays a critical role in the research fields such as the development of artificial cell chassis, tissue models, engineering drug delivery systems, and drug screening tools. However, achieving this goal is extremely challenging. The complexity of cell structures and the miniaturization and diversity of basic modules pose high requirements for the construction methods. The microfluidic chip, as an advanced microanalysis system, serves as an effective tool for building artificial cells. It can accurately control the structure and local microenvironment of artificial cells, becoming the preferred approach for the current research on synthetic life. This article reviewed the methods of constructing, manipulating, and analyzed artificial cells based on microfluidic chips, emphasized the importance of the microenvironment for life systems and artificial self-sustaining systems. In addition, this article demonstrated the wide applications of artificial cells in multiple critical biomedical fields. Exploring the advantages, disadvantages, and application performance of different microfluidic methods can enrich our knowledge about artificial cell research. Finally, we made an outlook on the development of artificial cell research based on microfluidics, expecting that this field can achieve greater breakthroughs and progress.

Application of Cyanobacteria as Chassis Cells in Synthetic Biology.

Synthetic biology is an exciting new area of research that combines science and engineering to design and build new biological functions and systems. Predictably, with the development of synthetic biology, more efficient and economical photosynthetic microalgae chassis will be successfully constructed, making it possible to break through laboratory research into large-scale industrial applications. The synthesis of a range of biochemicals has been demonstrated in cyanobacteria; however, low product titers are the biggest barrier to the commercialization of cyanobacterial biotechnology. This review summarizes the applied improvement strategies from the perspectives of cyanobacteria chassis cells and synthetic biology. The harvest advantages of cyanobacterial products and the latest progress in improving production strategies are discussed according to the product status. As cyanobacteria synthetic biology is still in its infancy, apart from the achievements made, the difficulties and challenges in the application and development of cyanobacteria genetic tool kits in biochemical synthesis, environmental monitoring, and remediation were assessed.

Development and characterization of tobacco suspension cell chassis NBS-1

Plant synthetic biology has significant theoretical advantages in exploration and production of plant natural products. However, its contribution to the field of biosynthesis is currently limited due to the lack of efficient chassis systems and related enabling technologies. Synthetic biologists often avoid tobacco as a chassis system because of its long operation cycle, difficulties in genetic and metabolic modification, complex metabolism and purification background, nicotine toxicity, and challenges in accurately controlling for agricultural production. Nevertheless, the tobacco suspension cell chassis system offers a viable solution to these challenges. The objective of this research was to develop a tobacco suspension cell chassis with high scientific and industrial potential. This chassis should exhibit rapid growth, high biomass, excellent dispersion, high transformation efficiency, and minimal nicotine content. Nicotiana benthamiana, which has high applicability in molecular technology, was used to induce suspension cells. The induced suspension cells, named NBS-1, exhibited rapid growth, excellent dispersion, and high biomass, reaching a maximum biomass of 476.39 g/L (fresh weight), which was significantly higher than that of BY-2. The transformation efficiency of the widely utilized pEAQ-HT transient expression system in NBS-1 reached 81%, which was substantially elevated compared to BY-2. The metabolic characteristics and bias of BY-2 and NBS-1 were analyzed using transcriptome data. It was found that the gene expression of pathways related to biosynthesis of flavonoids and their derivatives in NBS-1 was significantly higher, while the pathways related to alkaloid biosynthesis were significantly lower compared to BY-2. These findings were further validated by the total content of flavonoid and alkaloid. In summary, our research demonstrates NBS-1 possesses minimal nicotine content and provides valuable guidance for selecting appropriate chassis for specific products. In conclusion, this study developed NBS-1, a tobacco suspension cell chassis with excellent growth and transformation, high flavonoid content and minimal nicotine content, which has important guiding significance for the development of tobacco suspension cell chassis.

Research on Running Performance Optimization of Four-Wheel-Driving Ackerman Chassis by the Combining Method of Quantitative Experiment with Dynamic Simulation

The wheeled chassis, which is the carrying device of the existing handling robot, is mostly only suitable for flat indoor environments and does not have the ability to work on outdoor rugged terrain, greatly limiting the development of chassis driven handling robots. On this basis, this paper innovatively designs a four-wheel-driving Ackerman chassis with strong vibration absorption and obstacle surmounting capabilities and conducts performance research and optimization on it through quantitative experiments and dynamic simulation. Firstly, based on the introduction of the working principle and structure of the four-wheel-driving Ackerman carrier chassis, a multi-sensor distributed dynamic performance test system is constructed through the analysis of the chassis performance evaluation index. Then, according to the quantitative operation experiment of the chassis, the vibration and acceleration characteristics of the chassis at different positions of the chassis, the amount of slip and straightness of the chassis under different running distance, and the operating characteristics of the chassis under different road conditions and different damping springs conditions were analyzed respectively, which verified the rationality of the chassis design. Finally, by constructing the chassis dynamics simulation model; the influence law of chassis structure; and performance parameters such as chassis wheelbase, guide rod structure, and parameters, wheel friction coefficient and assembly error on the dynamic characteristics of the chassis is studied, and the optimal structure of the four-wheel-driving Ackerman chassis is determined while it is verified based on the simulation results. The research shows that the four-wheel-driving Ackerman chassis has good vibration performance and stability and has strong adaptability to different roads. After optimization, the vibration performance, stability, amount of slip, and straightness of the chassis structure are significantly improved, and the straightness is reduced to 0.399%, which is suitable for precise carriage applications on the chassis. The research has important guiding significance for promoting the development and application of wheeled chassis.

Finite element analysis and optimization of a Formula SAE car chassis

In motorsport engineering, achieving the ideal balance between speed, agility, and safety is a monumental challenge. Formula SAE competitions, where collegiate racing teams design high-performance vehicles, emphasize the pivotal role of the chassis, the backbone of the racing machine. Ensuring chassis compliance with regulations and optimizing structural integrity and weight is a formidable task. This study addresses this multifaceted challenge by focusing on the development, simulation, and optimization of a Formula SAE car's chassis. Rigorous analysis of four chassis versions seeks to strike a balance between rigidity and weight, enhancing performance while adhering to rules. Longitudinal torsion, diamond torsion, lateral acceleration, and braking tests assess structural rigidity and stress distribution, ensuring predictability and preventing potential failures. The mesh uses one-dimensional elements (1 mm size) and material properties akin to SAE 1020 steel. The fourth iteration consistently demonstrated superior performance across all tests. Despite a slight weight increase, it exhibited a longitudinal torsional rigidity of 1985 Nm/deg, well-aligned with Formula SAE standards, and a formidable diamond torsional rigidity of 56,304 Nm/deg. Critical stress analysis during lateral acceleration and braking tests remained well within permissible limits, with a chassis weight of approximately 35 kg. In summary, this work meets stringent Formula SAE standards, offering a systematic methodology for chassis design and optimization. The fourth iteration excels in various critical tests and serves as a valuable contribution to motorsport engineering, providing a blueprint for enhanced competitiveness and performance in the 2023 Formula SAE Brazil competition.

Development and evaluation of 4WSS electric-driven chassis for high-clearance sprayer.

The high clearance sprayer with conventional steering mechanisms, as an intelligent spraying machine, is frequently stuck or broken in muddy fields due to the excessive torque load. A Four-Wheel Self-Steering (4WSS) electric-driven chassis with a smaller turning radius and better passability is developed to handle complex agricultural terrains. The 4WSS chassis is mainly composed of two custom-designed steering bridges and four in-wheel drive motors. It can achieve steering and driving forward simultaneously through coordinate differential speed control of drive motors, saving a set of dedicated servo steering systems and requiring less torque during steering compared to conventional structures. A kinematic model depicting the speed relationships between four wheels is established via geometric analysis, and a Speed Distribution Controller (SDC) is designed to accomplish locomotion objectives. Experimental results demonstrate the effectiveness of the new prototype 4WSS chassis system in tracking speed and steering angle. Compared to conventional agricultural chassis, the 4WSS chassis has a smaller turning radius of 2,877 mm. The 4WSS chassis exhibits superior performance in typical field conditions, including muddy terrain, deep gullies, and ridges.

Development of an Attitude Adjustment Crawler Chassis for Combine Harvester and Experiment of Adaptive Leveling System

Body tilt is typically unavoidable for agricultural vehicles or platforms when operated over tough terrain in agricultural production, and, therefore, it is not possible to guarantee the operation performance. Current leveling methods mainly focus on wheeled vehicles or adjustment for single working parts rather than the vehicle body, and few of them could be applied to crawler vehicles. The objective of this research was to put forward an adaptive leveling system for combine harvesters based on the development of a new four-point lifting adjustable crawler chassis. The working principle of the proposed adjustable mechanism was studied on the basis of the analysis of adjustment characteristics under multiple posture adjustment conditions in a RecurDyn environment. The design relies on the combination of the attitude detection of the leveling system and adjustment calculation of driving hydraulic cylinders according to the established mathematical models. Within the designed adjustable extent (including adjustment range of vehicle height 0–87.8 mm, lateral inclination ±3.98° and longitudinal inclination −2.9–5.2°), the leveling performance of lateral and longitudinal inclination was then tested separately. The experiment showed that the leveling system could achieve automatic leveling with an accuracy of ±0.4° and could provide technical support for the development of crawler vehicles.

Development of vehicle chassis manufactured by 3D pultrusion

The present work presents a preliminary study on the development of a tubular chassis design for automotive industry manufactured with ultraviolet (UV) 3D pultrusion. The use of straight profiles for energy absorption elements and the advantages and feasibility of using curved profiles in other structural areas have been analysed. For the straight profiles, the energy absorption cabability of the material (specific energy absorption, SEA) has been evaluated performing quasi-static compression tests, showing high energy dissipation values, over 50 kJ / kg. On the other hand, the manufacturing of curved profiles by UV pultrusion have been analysed, evaluating the influence of the processing parameters (emitting intensity and pulling speed) and the influence of the reinforcement type on the geometry of the profile. It has been observed that the roving reinforcement allows an easier manipulation and allows to obtain curves without wrinkles with complex sections, due to the free movement between yarns; while the tape reinforcement may present wrinkles during the curving stage. In addition, based on a design with straight profiles, it has been shown that using non-rectilinear profiles, a significant reduction of the necessary connection nodes can be achieved, reducing up to 75% the number of connection nodes based on the analyed design.

Streptomyces as Microbial Chassis for Heterologous Protein Expression.

Heterologous production of recombinant proteins is gaining increasing interest in biotechnology with respect to productivity, scalability, and wide applicability. The members of genus Streptomyces have been proposed as remarkable hosts for heterologous production due to their versatile nature of expressing various secondary metabolite biosynthetic gene clusters and secretory enzymes. However, there are several issues that limit their use, including low yield, difficulty in genetic manipulation, and their complex cellular features. In this review, we summarize rational engineering approaches to optimizing the heterologous production of secondary metabolites and recombinant proteins in Streptomyces species in terms of genetic tool development and chassis construction. Further perspectives on the development of optimal Streptomyces chassis by the design-build-test-learn cycle in systems are suggested, which may increase the availability of secondary metabolites and recombinant proteins.

Mechanical strength study of Off-Road vehicle chassis body materials

The design and development of vehicle chassis is essential part of automobile production sector because the vehicle self-weight and entire external load (including passenger weight) are built on the chassis frame. The chassis is a significant integral part of the vehicle. The design and development of Off-road vehicle (ORV) are to be influenced by design criteria, its material selection, and components convenience. This paper is covering the material behaviour study of ORV chassis in terms of mechanical strength, durability, fatigue, etc. The result of proposed work is get the best techniques to build the safest roll cage (RC) as per material selection, designing, ergonomics, manufacturability and different parameters that have to be kept in intellect whereas planning an off-road vehicle. This venture will accomplish an off-road vehicle that looks aggressive and at the same time keeps the driver and other crucial constituents like motor, powertrain etc. unharmed interior of the RC. The 22 commercial engineering materials have been investigated for chassis development, mainly AISI 4130 material is most popular due to high tensile strength in all available materials. The ANSYS model of tubular chassis frame has been demonstrated in terms of front, side and rare impact load according to SAE vehicle development norms.

Design and development of hybrid chassis for two wheeler motorcycle

The primary objective of this work is to design and develop a hybrid chassis for a two-wheeler motorcycle. Perimeter and Trellis are the most commonly used chassis types in motorcycles. Designing a new hybrid chassis of a two-wheeler for better high-speed stability and agility. Trellis frame is meant for good stiffness and rigidity, which is required for track scenarios, trellis frame consists of metal tubes arranged in triangulated reinforcements, these triangulated trusses eliminate additional resonance frequencies. While a Perimeter frame has a bit of flex and are compact in nature which is used for road-going scenarios. However, chassis stiffness is the most fascinating area of chassis design, as it reduces the overall vibrations induced in a frame which helps in having multiple ways to mount the engine depending on the resonance factors. Engineers want the frame and ancillaries to offer excellent longitudinal stiffness, so the bike stays straight and stable while pulling 1.5g during braking, and they also want enough stiffness so the bike reacts to rider steering inputs. By combining both the parameters and characteristics of perimeter and trellis frame, vibrations can be reduced and both stability and agility are achieved.

Dataset for supporting a modular autoinduction device for control of gene expression in Bacillus subtilis.

Modular and tuneable genetic tools for Metabolic Engineering fuels the development of chassis for the efficient production of biocompounds at industrial scale. We have constructed an autoinduction device for gene expression in Bacillus subtilis based on the LuxR/I quorum sensing system [1]. Here, we present raw and processed data regarding to B. subtilis growth measured as OD600, performed in three different scales: microcultivation on 96-well plates (200 µL), test tubes (12 mL), and Erlenmeyer flasks (50 mL). We also present raw and processed data on gene expression measured as GFP fluorescence (485/535 nm), luminescence and riboflavin production. Measurements were performed on a microplate reader Tecan 200 PRO (iControl software) and on spectrophotometer (Thermo Fisher Scientific GENESYS 10S UV-Vis). Processed data are presented as product/OD600, maximum and minimum promoter activity, fold of induction, and the induction OD600.

Development of an unmanned tractor chassis for precision farming

The development of the chassis of an unmanned tractor for a local complex of precision fanning is considered. The design is based on the kinematic scheme of the shaft gearbox, which implements a large number of modes and the principle of two-line transmission. A feature of this scheme is the presence of parallel load shafts. Keywords: wheeled tractor, autonomous control, two-line transmission, slipping, disc friction clutch. sergey_voi@mail.ru

Design & Development of Electric Two-Wheeler Chassis

Design & Development of Electric Two-Wheeler Chassis

Development of a four-point adjustable lifting crawler chassis and experiments in a combine harvester

In agriculture, the body tilt of vehicles or platforms can be controlled by levelling technology to prevent rollover and improve efficiency when operated in rough terrain. Many researchers have carried out important study in this area based on mechanism development and optimization, but most of them are not effective to crawler vehicles. The objective of this research was to specifically develop a levelling mechanism for crawler combine harvester, since as a consequence, enhancing the operation performance and trafficability. This levelling method depends on a hydraulic four-point adjustable lifting chassis, which could adjust vehicle chassis by controlling hydraulic cylinders. Therefore, the mathematical models between chassis attitude (including ground clearance, the horizontal and longitudinal inclination angle) and each length of hydraulic cylinder were deduced. The principle of lifting mechanism of crawler vehicle chassis was analyzed by using virtual prototyping software ADAMS, as well as dynamic performance under three different attitude adjustment conditions. The maximum adjustment range of the ground clearance is 140 mm and the lateral inclination adjustment range is ±5.17°. Experiment showed that the designed levelling mechanism was feasible and with positive significance for crawler vehicles levelling.

Performance Analysis and Platform Development of Passenger Car Chassis

This thesis exploits chassis dynamic performance analysis platform of the passenger carmodule on the basis of based on the VC#.Net software. Its purpose is to significantly shorten thedevelopment cycle of other models located on the same platform in the process of change. It mainlyadopts the interactive form of menu and dialogue to build the platform. Through the way by usinginternal call Adams/Car module to build a similar Car model file and altering some design variables,the suspension and chassis models of required models can be built quickly. In accordance with thepre-designed suspension and vehicle stability analysis project as the model, it achieves rapidsimulation analysis and access to the analysis results in time. The accuracy of the platform wasproved by the K&C test of the developed model. It provides great reference significance for laterproduct development.

Electric Car Chassis for Shell Eco Marathon Competition: Design, Modelling and Finite Element Analysis

The increasing demand for energy efficient electric cars, in the automotive sector, entails the need for improvement of their structures, especially the chassis, because of its multifaceted role on the vehicle dynamic behaviour. The major criteria for the development of electric car chassis are the stiffness and strength enhancement subject to mass reduction as well as cost and time elimination. Towards this direction, this work indicates an integrated methodology of developing an electric car chassis considering the modeling and simulation concurrently. The chassis has been designed in compliance with the regulations of Shell Eco Marathon competition. This methodology is implemented both by the use of our chassis load calculator (CLC) model, which automatically calculates the total loads applied on the vehicle’s chassis and by the determination of a worst case stress scenario. Under this extreme stress scenario, the model’s output was evaluated for the chassis design and the FEA method was performed by the pre-processor ANSA and the solver Ansys. This method could be characterized as an accurate ultrafast and cost-efficient method.

Experimental Verification of the Steering Performance of All-Hydraulic Crawler Chassis

The force conditions in the steering system of the chassis under different conditions are analyzed theoretically for the independently designed and developed all-hydraulic crawler chassis. Using the multi-body dynamic simulation software RecurDyn, the chassis steering performance on sandy loam and clay pavements, and the steering performance under different steering radiuses on the sandy loam pavement are simulated and analysed dynamically respectively. The steering resistance moment is studied when the pavement conditions and steering radius are different. This research selects inside and outside crawler slip ratio as an index, and road conditions, speed and steering radius as factors to test the steering performance of all-hydraulic crawler chassis under different operating conditions. It is observed from the simulation results that during the pivot steering on the sandy loam, the drive torque and braking torque of the driving wheel are larger than on the clay ground. With the decrease of the steering radius, the torques of the left and right driving wheel are both gradually increasing. In the same steering radius, the torque of the outside driving wheel is larger than that of the inside driving wheel. The simulation results are consistent with the theoretical analysis results. In the steering performance test, the factors influencing the slippage rate on both sides of the crawler are such that the influence of the steering radius is greater than that pf the pavement condition and the pavement condition influence is greater than that of the speed. Among them, the steering radius has a significant influence on the slip ratio of the inside crawler, and an extremely significant influence on the slip ratio of the outside crawler. This research can provide a certain theoretical basis and technical reference for the development of hydraulic crawler chassis and optimization of the steering system.

Genetic tools for advancement of Synechococcus sp. PCC 7002 as a cyanobacterial chassis.

BackgroundSuccessful implementation of modified cyanobacteria as hosts for industrial applications requires the development of a cyanobacterial chassis. The cyanobacterium Synechococcus sp. PCC 7002 embodies key attributes for an industrial host, including a fast growth rate and high salt, light, and temperature tolerances. This study addresses key limitations in the advancement of Synechococcus sp. PCC 7002 as an industrial chassis.ResultsTools for genome integration were developed and characterized, including several putative neutral sites for genome integration. The minimum homology arm length for genome integration in Synechococcus sp. PCC 7002 was determined to be approximately 250 bp. Three fluorescent protein reporters (hGFP, Ypet, and mOrange) were characterized for gene expression, microscopy, and flow cytometry applications in Synechococcus sp. PCC 7002. Of these three proteins, the yellow fluorescent protein (Ypet) had the best optical properties for minimal interference with the native photosynthetic pigments and for detection using standard microscopy and flow cytometry optics. Twenty-five native promoters were characterized as tools for recombinant gene expression in Synechococcus sp. PCC 7002 based on previous RNA-seq results. This characterization included comparisons of protein and mRNA levels as well as expression under both continuous and diurnal light conditions. Promoters A2520 and A2579 were found to have strong expression in Synechococcus sp. PCC 7002 while promoters A1930, A1961, A2531, and A2813 had moderate expression. Promoters A2520 and A2813 showed more than twofold increases in gene expression under light conditions compared to dark, suggesting these promoters may be useful tools for engineering diurnal regulation.ConclusionsThe genome integration, fluorescent protein, and promoter tools developed in this study will help to advance Synechococcus sp. PCC 7002 as a cyanobacterial chassis. The long minimum homology arm length for Synechococcus sp. PCC 7002 genome integration indicates native exonuclease activity or a low efficiency of homologous recombination. Low correlation between transcript and protein levels in Synechococcus sp. PCC 7002 suggests that transcriptomic data are poor selection criteria for promoter tool development. Lastly, the conventional strategy of using promoters from photosynthetic operons as strong promoter tools is debunked, as promoters from hypothetical proteins (A2520 and A2579) were found to have much higher expression levels.Electronic supplementary materialThe online version of this article (doi:10.1186/s12934-016-0584-6) contains supplementary material, which is available to authorized users.

Mechatronic design and development of a non-holonomic omnidirectional mobile robot for automation of primary production

AbstractThis paper presents the mechatronic system design and development of a four-wheel drive/steer (4WD4S) mobile robot as a non-holonomic omnidirectional robot, MARIO—Mobile Autonomous Robot for Intelligent Operations. An innovative integrated application of CAD/CAM/CAE and RP has been employed for rapid development of the robot chassis and other mechanical parts by using different software tools. Most of the parts were designed by 3D CAD software which allows further CAE analysis including structural and motion analysis. To reduce the manufacturing time and cost, CAM and RP have been used to manufacture the main parts. These master parts are manufactured by workshop machining and 3D printing. Robot Operating System (ROS) alongside Gazebo simulator have been utilized for modelling, simulation, software development and visualization which enabled to develop the optimum embedded system to control the robot. With this integrated approach, a prototype of MARIO has been developed with a reasonably low manu...