Development Of Animal Husbandry Research Articles

Comparison of Production Performance, Slaughtering Performance and Meat Quality of Qinghai Plateau-Type Yaks of Different Months of Age

Background: Research and development in animal husbandry is receiving increasing attention as society continues to develop and as consumer awareness of the nutritional value of livestock products, information about their sources and the importance of production systems and environmental impacts increases. In this context, research on the production performance, slaughtering performance and meat quality of Qinghai plateau-type yak, as a rare and important livestock resource, has become essential and the market demand for high-quality yak meat products has increased significantly. Methods: 10 yaks of 3, 4 and 5 months of age were selected at the same feeding level and 3 yaks of different months of age were randomly selected for slaughtering to determine the routine performance and meat quality and to evaluate them. Result: The results showed that the pH of Qinghai Plateau-type yak meat differed significantly (P less than 0.05) among yaks of different ages. There was a significant difference in the ash content of the longest dorsal muscle of yaks with increasing months of age (P less than 0.05) and the water content of yak meat at 5 months of age decreased significantly (P less than 0.05). The difference in arm triceps muscle was more significant (P less than 0.05) in yaks of different months of age. There was no significant difference (P greater than 0.05) in drip loss rate and moisture content of other parts of different months of age.

Assessment of grassland carrying capacity drivers and evaluation of pasture-livestock balance: A case study of Xinjiang, China

Evaluating the spatiotemporal variations and driving mechanisms of carrying capacity (CC) is critical for optimizing grassland resource utilization and promoting sustainable development in grassland animal husbandry. This study focuses on Xinjiang, an arid and semi-arid region significantly impacted by environmental changes and human activities. To capture the dynamics of grassland CC, we employed Theil-Sen trend analysis and the Mann-Kendall test to assess spatiotemporal trends. The innovative use of the Geodetector method allowed for a detailed quantification of the influence of various environmental and human-related factors on grassland CC. Additionally, we incorporated livestock data to evaluate the overgrazing conditions of the grassland ecosystem. Our findings demonstrate that: (1) Grassland CC has showed a pronounced overall upward trajectory over the last twenty years, with notable inter-annual fluctuations and significant spatial variations, particularly between the northern and southern regions. (2) The spatial distribution of grassland CC is primarily influenced by precipitation patterns and population density, with key determining factors varying across different types of grasslands. Except for alpine steppe and alpine desert, the spatial distribution of grassland CC was primarily influenced by two-factor interactions, surpassing the impacts of single-factor effects. (3) The overgrazing rate has generally declined, peaking in 2014 and showing the mildest condition in 2017. In comparison, the overgrazing situation in Northern Xinjiang is relatively more favorable, whereas in the southern and eastern regions it necessitates more immediate and comprehensive ecological restoration and management measures. In summary, this study provides important scientific evidence for prioritizing grassland protection and planning for the sustainable animal husbandry development in arid and semi-arid regions.

Birds of prey in the historical past of Eastern Europe: Evidence from bones

The results of the study of birds of prey remains from the southern part of Eastern Europe are analysed in the paper. The studied material comes from 169 archaeological sites of different age (Neolithic/Eneolithic, Bronze Age, Early Iron Age, Early Middle Ages, Kyivan Rus Time, and the Modern Age). Twenty-eight species of raptors were identified in the samples. Of them, hawks, eagles, and vultures are represented by 20 species, while falcons and owls are less diverse (each are represented by only four taxa). Particular birds of prey species were more widely distributed in the historical past of the studied region and were characterised by higher abundance compared to modern times. The remains of owls are especially numerous in materials from Neolithic, Eneolithic, and Bronze Age settlements. The Early Iron Age was the time of a high abundance of diurnal raptors, which were used for different purposes by the people inhabiting the territory of modern-day Ukraine. Only a few birds of prey were recorded for the Early Middle Ages and the Modern Age, most probably due to economic issues and the development of animal husbandry. Numerous remains of hawks and a lesser amount of falcon bones were found at settlements of the Kyivan Rus state, which is associated with the spread of gamehawking while falconry with falcons was available mainly to the elite of that time. The results of archaeornithological studies are supplemented and corroborated to some extent with the information taken from literature sources and by analysing the ancient images of birds.

Application of GWAS and mGWAS in Livestock and Poultry Breeding.

In recent years, genome-wide association studies (GWAS) and metabolome genome-wide association studies (mGWAS) have emerged as crucial methods for investigating complex traits in animals and plants. These have played pivotal roles in research on livestock and poultry breeding, facilitating a deeper understanding of genetic diversity, the relationship between genes, and genetic bases in livestock and poultry. This article provides a review of the applications of GWAS and mGWAS in animal genetic breeding, aiming to offer reference and inspiration for relevant researchers, promote innovation in animal genetic improvement and breeding methods, and contribute to the sustainable development of animal husbandry.

The genetic characterization of germplasm and identification of the litter size trait associated candidate genes in Dexin mutton and fine-wool sheep.

Xinjiang is a major province of sheep breeding in China, which plays an important role in meeting people's needs for meat products, increasing farmers' income and sustainable development of animal husbandry. However, the genetic differentiation relationship between breeds was not clear, and most sheep had low fecundity, which seriously restricted the efficient development of sheep industry. Therefore, this study used the whole genome resequencing to detect the genetic variation of Dexin mutton and fine-wool sheep, explored the selected regions and important genes of the litter size traits, analyzed the genetic mechanism of reproductive traits, and provided new insights for the high fecundity breeding of sheep. A total of 5,236.338 G genome data and 35,884,037 SNPs were obtained. Furthermore, we identified 39 selection signals spanning candidate genes, 99 genes were significantly associated related to growth, reproduction and immunity, among which, BRIP1, BMPR1B, BMP4, NGF, etc. genes, and MAKP signaling pathway, Fanconi anemia pathway and Thyroid hormone signaling pathway and other signaling pathways were significantly correlated with litter size trait. Among them, we identified NGF, TrKA and BRIP1 genes was the important genes for sheep litter size traits and the mutation frequencies of 9 SNPs in BRIP1 gene were significantly different in domestic sheep in the world. The research provided new insights for the breeding of self-cultivated meat fine-wool sheep.

Surface Display of Cholera Toxin B Subunit Recombinant Escherichia coli Ghosts Further Enhances Resistance to Chlamydia abortus Infection in Mice.

Chlamydia abortus (C. abortus) is an important zoonotic pathogen that seriously endangers the development of animal husbandry. Vaccination is the most effective approach to preventing C. abortus infection. We previously reported a recombinant Escherichia coli ghost (rECG)-based C. abortus vaccine that demonstrated outstanding protective efficacy. In this study, we further attempted to fuse the cholera toxin B subunit (CTB), a widely studied potent mucosal immune adjuvant, with macrophage infectivity potentiator (MIP), a candidate antigen of C. abortus, on the surface of the rECG and explore its protective effect against C. abortus infection. The MIP fusion protein was highly expressed in the rECGs, and the CTB-modified rECGs significantly induced the activation of mouse bone marrow-derived dendritic cells in vitro. Intranasal immunization with rECGs induced a Th1-biased cellular immune response. Compared to the rECGs without CTB, the CTB-modified rECGs induced higher concentrations of IgA in the serum and vaginal wash solution. Moreover, in a mouse infection model, the CTB-modified rECGs significantly improved the clearance efficiency of C. abortus and reduced the pathological damage to the uterus. This study demonstrates that incorporating CTB into rECGs significantly enhances the immunogenic potential of the rECG vaccine and can significantly enhance its protective efficacy against a C. abortus challenge.

Mangosteen extract reduces the bacterial load of eggshell and improves egg quality

The increasing emergence and spread of antibiotic resistance accelerate the desire for antibiotic alternatives. Plant extracts have emerged as a promising and relatively unexplored area of research as potential substitutes. Herein, we investigated the prevalence and distribution patterns of bacteria on egg surfaces and evaluated the inhibitory effects of mangosteen extract on these surface bacteria. In addition, we examined the antioxidant activity and egg quality in improving the ability of mangosteen extract. The results showed that the predominant bacteria isolated from eggshells were Gram-positive, with Staphylococcus and Micrococcus as the dominant genera. Notably, mangosteen extract exhibited significant bactericidal activity, effectively inhibiting Gram-positive bacteria on the surface of chicken eggshells. Moreover, the supplementation of mangosteen extract in the feed of laying hens yielded a noteworthy improvement in egg quality, accompanied by positively shaped structure and function of microbial communities on the egg surface and in the feces. Collectively, our findings suggested that mangosteen extract was an effective alternative to traditional antibiotics, offering valuable insights for animal husbandry development.

Herders' risk preference and grassland transfer strategy: Evidence from a field experiment in pastoral areas of China

AbstractEncouraging and guiding herders to participate in grassland transfer promotes the high‐quality development of grassland animal husbandry. We conducted a survey among 356 herders from Qinghai and Gansu provinces in China, to explore the impact of herders' risk preference and perceptions on their grassland transfer strategies. We used experimental economics methods to measure the risk preferences of herders. Our results indicate that the majority of herders exhibit risk‐averse attitudes. A theoretical model was used to establish hypotheses on the relationship between risk preference and grassland transfer decision‐making. The double‐hurdle model and moderating effect model were used to test these hypotheses. Our analysis shows that herders' risk preferences have a significant negative effect on their decisions to engage in grassland transfer. Both risk preferences and risk perception have a significant impact on grassland transfer‐in decisions, but no significant impact on grassland transfer‐out. A heterogeneity analysis revealed that the impact of risk preferences on grassland transfer participation is more pronounced among herders in Qinghai compared to those in Gansu. Moreover, we found that risk perception plays a moderating role on the influence of risk preferences on decisions regarding grassland transfer. These findings are crucial to formulate strategies for the sustainable management of the grassland transfer market.

Exploring the Water-Soil-Crop Dynamic Process and Water Use Efficiency of Typical Irrigation Units in the Agro-Pastoral Ecotone of Northern China.

Groundwater resources serve as the primary source of water in the agro-pastoral ecotone of northern China, where scarcity of water resources constrains the development of agriculture and animal husbandry. As a typical rainfed agricultural area, the agro-pastoral ecotone in Inner Mongolia is entirely dependent on groundwater for agricultural irrigation. Due to the substantial groundwater consumption of irrigated farmland, groundwater levels have been progressively declining. To obtain a sustainable irrigation pattern that significantly conserves water, this study faces the challenge of unclear water transport relationships among water, soil, and crops, undefined water cycle mechanism in typical irrigation units, and water use efficiency, which was not assessed. Therefore, this paper, based on in situ experimental observations and daily meteorological data in 2022-2023, utilized the DSSAT model to explore the growth processes of potato, oat, alfalfa, and sunflower, the soil water dynamics, the water balance, and water use efficiency, analyzed over a typical irrigation area. The results indicated that the simulation accuracy of the DSSAT model was ARE < 10%, nRMSE/% < 10%, and R2 ≥ 0.85. The consumption of the soil moisture during the rapid growth stage for the potatoes, oats, alfalfa, and sunflower was 7-13% more than that during the other periods, and the yield was 67,170, 3345, 6529, and 4020 kg/ha, respectively. The soil evaporation of oat, potato, alfalfa, and sunflower accounted for 18-22%, 78-82%; 57-68%, and 32-43%, and transpiration accounted for 40-44%, 56-60%, 45-47%, and 53-55% of ETa (333.8 mm-369.2 mm, 375.2 mm-414.2 mm, 415.7 mm-453.7 mm, and 355.0 mm-385.6 mm), respectively. It was advised that irrigation water could be appropriately reduced to decrease ineffective water consumption. The water use efficiency and irrigation water use efficiency for potatoes was at the maximum amount, ranging from 16.22 to 16.62 kg/m3 and 8.61 to 10.81 kg/m3, respectively, followed by alfalfa, sunflowers, and oats. For the perspective of water productivity, it was recommended that potatoes could be extensively cultivated, alfalfa planted appropriately, and oats and sunflowers planted less. The findings of this study provided a theoretical basis for efficient water resource use in the agro-pastoral ecotone of Northern China.

СТРАТЕГИЧЕСКОЕ УПРАВЛЕНИЕ УСТОЙЧИВЫМ РАЗВИТИЕМ ОТРАСЛИ ЖИВОТНОВОДСТВА НА РЕГИОНАЛЬНОМ УРОВНЕ

The livestock industry is a fundamental part of agriculture. The relevance of developing a sustainable livestock development system is due to a number of global and local factors that have a significant impact on modern agricultural systems. The article examines the current state of the livestock industry in the Russian Federation, as well as in the Nizhny Novgorod region. Ensuring the sustainable development of animal husbandry is an important aspect in the formation and assessment of the country's food security. Criteria for the sustainable development of the industry can serve not only economic indicators, including profitability, gross profit, etc., as well as indicators of social sustainability, which include the quality of life of the population, the development of human resources, etc., but also directly indicators characterizing the production of livestock products, the preservation of animal health, etc. In this regard, measures to ensure sustainable development are of particular relevance. In order to determine the main directions of development, we have compiled a strategic map. The strategic map serves as a tool for visualizing, planning and implementing a development strategy, taking into account many factors and stakeholders. The development of a strategic map for the sustainable development of livestock is a key element for achieving a balance between economic efficiency, social justice and environmental sustainability. A methodological tool for drawing up a strategic map has been developed, providing a systematic approach to the issue under consideration with the allocation of the following "pools": human resources, innovation, financing, as well as active marketing.

History, status and genetic characteristics of native cattle breeds from the Republic of Kazakhstan.

This work provides a comprehensive review of the history, status, and genetic characteristics of cattle breeds in Kazakhstan. The current breeding status is analysed, including information on popular breeds such as Kazakh white-headed, Auliekol, Alatau, Aulieata, and Kalmyk, their production and economic significance. An overview of genetic studies using DNA fingerprinting, microsatellites, and SNPs aimed at identifying unique characteristics, genetic diversity, and genes under selection, as well as markers of economically important and productive traits of Kazakh cattle breeds, is also provided. The study examined the genetic structure of the Kazakh white-headed and Alatau breeds based on whole-genome SNP genotyping. Unique genetic components characterizing Kazakhstan cattle breeds were described, and comparisons were made with genetic data from other breeds. Structural analysis showed that the Kazakh white-headed breed contains genetic components of the Hereford, Kalmyk, and Altai cattle. The Alatau breed has a composite structure, containing components of the Brown Swiss, Braunvieh, Kalmyk, and Holstein breeds. The results not only reveal the genetic diversity and characteristics of cattle breeds in Kazakhstan and the historical development and current state of animal husbandry in the country, but also emphasize the importance of further research to identify adaptive and unique genetic markers affecting economically important traits of local breeds.

LIVESTOCK DEVELOPMENT IS AN INTEGRAL PART OF THE SUSTAINABLE DEVELOPMENT OF THE UKRAINIAN COUNTRYSIDE AND THE COUNTRY’S FOOD SECURITY

Agricultural production is one of the most important components of the economy of every country, as it guarantees their food security. The livestock products market occupies an important place in the general food market of Ukraine. Ensuring the needs of the population with food products produced from the products provided by the livestock industry depends on its development. However, the actual state of the industry during the last 30 years does not correspond to potential opportunities and is characterized by a reduction in the number of almost all types of farm animals. In the 90s of the last century, after a short-lived reform of the agro-industrial complex, livestock farming in Ukraine underwent negative changes. The population of agricultural animals in large agricultural enterprises (collective farms and state farms) began to decrease at a catastrophic pace, productivity decreased, production became unprofitable and product quality deteriorated, the country’s export potential decreased and the social living conditions of the rural population worsened. All this puts national food security at risk. The article analyzes the state of animal husbandry development in the period 1991–2021 and found that after 1990, the production of the main livestock products moved to private homesteads of peasants, to small areas of homestead plots. Most of the peasants in their households keep pigs (68.4%), sheep and goats (78%), poultry (79.5%), rabbits (99.5%) and bee colonies (88.3%). It was established that in 2021, the production of meat and meat products amounted to 70.1%, milk and dairy products — 44.6% of those produced in 1991, but the production of eggs increased by 8.3% compared to 1991. It was found that the low level of income does not allow Ukrainians to eat well. In 2021, the level of consumption of meat and meat products by the population of Ukraine amounted to 71% of the rational norm and exceeded the minimum necessary norm by only 9.2%. Ukrainians also do not consume enough milk and dairy products — 53.1% of the rational norm, and only eggs — an almost rational norm (95.9%). At the same time, it should be noted that 29% of the average daily diet of Ukrainians is provided by the consumption of products of animal origin. The influence of the enemy’s military actions on the livestock industry of Ukraine is given. Significant losses of farm animals and poultry and ecological damage to the natural environment for decades to come have been caused due to the destruction of animals and the inability to dispose of them in time and in accordance with the rules.

Research on the Composting Technology of Cattle and Sheep Manure Based on Intelligent and Efficient Composting Equipment and the Evaluation Standard of Decomposition Degree

The Inner Mongolia Autonomous Region is a crucial area in China with a significant advantage in animal husbandry, particularly in cattle and sheep farming. However, the disposal of the large quantities of manure produced during farming has severely impacted the industry’s healthy development. Proper treatment of the manure can convert it into organic fertilizer beneficial to farmland; otherwise, it will cause substantial environmental pollution. This study focuses on existing composting equipment and addresses the issues of cattle and sheep manure mixture ratios and compost maturity evaluation. Through experiments on the mixture of cattle and sheep manure, the optimal ratio for converting cattle and sheep manure into organic fertilizer was determined. Additionally, a fuzzy mathematical evaluation model was employed, along with experimental data, to establish a comprehensive evaluation system for aerobic compost maturity based on multiple indicators, revealing the variation patterns of maturity levels under different mixture ratios. The test results revealed that the composting equipment effectively controls the composting process, shortens the composting cycle, ensures the complete decomposition of organic matter, and meets national standards for livestock and poultry manure treatment. Regarding temperature and humidity, oxygen concentration, seed germination rate, pH value, electrical conductivity (EC), nitrogen, phosphorus, potassium content, and carbon-to-nitrogen ratio, the mixed compost of cattle and sheep manure in various ratios met the relevant standards for agricultural application. Various ratios of organic fertilizers containing cattle and sheep manure significantly promoted the growth of maize, wheat, and mung bean crops. Specifically, the compost decomposition cycle was shortest when sheep and cattle dung were mixed at a ratio of 2:1, while it was longest for all cattle dung. Finally, a fuzzy mathematical comprehensive evaluation model was established by selecting four indicators: water content, carbon-to-nitrogen ratio, apparent score, and germination index. The study demonstrates that the equipment and method offer significant advantages in efficiently treating cattle and sheep manure and producing organic fertilizer, thereby providing strong support for the sustainable development of animal husbandry.

Research on the impact of optimizing the use of grassland resources on promoting the development of animal husbandry on the Qinghai-Tibetan Plateau

The Qinghai Tibet Plateau, located in central Asia, is the highest plateau region in the world with an average altitude of over 4000 meters. Due to its unique geographical location and high altitude characteristics, the Qinghai Tibet Plateau is sometimes vividly referred to as the Roof of the World, and thus has been given the nickname Third Pole. The Qinghai Tibet Plateau provides a good foundation for the development of animal husbandry, but at the same time, due to the harsh climate conditions and degradation of grassland resources on the Qinghai Tibet Plateau, the development of animal husbandry is also facing a series of challenges. In order to increase the content of forage on the Qinghai Tibet Plateau and enrich its grassland resources. This article uses literature research method to conduct environmental analysis on animal husbandry in the Qinghai Tibet Plateau and other countries such as the United States. Comparative research method is used to analyze the differences in the development of animal husbandry in the Qinghai Tibet Plateau and other countries such as the United States. Interdisciplinary research method is used to propose solutions for the development of animal husbandry in the Qinghai Tibet Plateau (mainly for grassland development). That is, by selecting suitable grass species, establishing a grazing light complementary photovoltaic power station, and implementing grassland protection monitoring technology. Finally, the conclusions and shortcomings of the research were obtained

The contact toxicity and toxic mechanism of essential oils from Pimenta racemosa and Eugenia caryophyllata against Haemaphysalis longicornis (Acari: Ixodidae)

Haemaphysalis longicornis, which is widely distributed in China, can transmit various tick-borne diseases such as severe fever with thrombocytopenia syndrome, babesiosis, rickettsia disease and so on, and do great harm to human health and the development of animal husbandry. Chemical acaricides are the most traditional tick control method, but because of its many shortcomings, there is an urgent need to find a substitute with high efficiency, environmental protection and low toxicity. It has been found that some plant essential oils (EOs) have good insecticidal activity and environmental safety. In this study, the components of EOs from Pimenta racemosa and Eugenia caryophyllata were analyzed by gas chromatography–mass spectrometry (GC–MS), and their potential for application in the control of Haemaphysalis longicornis were studied. Gas chromatography-mass spectrometry analysis showed that the main components of P. racemosa EO were eugenol (64.07%), those of E. caryophyllata EO were Hexadecanoic acid, 2-methylpropyl ester (51.84%) and eugenol (39.76%). Larval packet test showed that the EOs of P. racemosa and E. caryophyllata had significant acaricidal activity against unfed larvae of H. longicornis, with LC50 values of 1.20 mg/mL and 0.47 mg/mL and LC90 values of 8.76 mg/mL and 2.91 mg/mL, respectively. The P. racemosa EO, E. caryophyllata EO and eugenol showed significant acaricidal activity against unfed nymph H. longicornis, with LC50 values of 1.65 mg/mL, 2.29 mg/mL and 0.93 mg/mL and LC90 values of 5.03 mg/mL, 11.01 mg/mL and 4.77 mg/mL, respectively. The P. racemosa EO, E. caryophyllata EO and eugenol showed significant acaricidal activity against unfed adults H. longicornis, with LC50 values of 0.51 mg/mL, 2.57 mg/mL and 1.83 mg/mL and LC90 values of 2.44 mg/mL, 11.44 mg/mL and 2.54 mg/mL, respectively. Enzyme assays revealed that the E. caryophyllata EO and eugenol significantly inhibited the activity of carboxylesterase (CarE), eugenol significantly inhibited the activity of catalase (CAT), and two EOs and eugenol had no significant effect on acetylcholinesterase (AchE) (p < 0.05). The above results suggest that the essential oils from P. racemosa and E. caryophyllata have great potential for use as alternatives to synthetic acaricides for tick control.

Effects of Organic Fertilizer Addition to Vegetation and Soil Bacterial Communities in Saline-Alkali-Degraded Grassland with Photovoltaic Panels.

The Songnen grassland is an important resource for livestock production in China. Due to the intensification of anthropogenic activities in recent years, vegetation degradation has worsened, and the salinization of grassland has become increasingly serious, which severely affects the sustainable development of grassland animal husbandry. In this study, organic fertilizer addition was carried out at saline-and-alkaline-degraded Songnen grassland sites with photovoltaic panels, and we investigated the effects of organic fertilizer treatments on the vegetation and soil bacteria in these areas. The results showed that both organic fertilizer treatments increased the community composition and diversity indices of plants (p < 0.05); they also had significant effects on soil electrical conductivity and rapidly available potassium (p < 0.05). In the dominant phylum of bacteria, the relative abundance of Firmicutes increased without adding organic fertilizer under the photovoltaic panel; the addition of organic fertilizer had a significant effect on the relative abundance of Firmicutes and Desulfobacterota (p < 0.05), reducing their relative abundance, respectively. There were differences in the number of bacteria at the genus level under different treatments compared to the control, with the highest enrichment of bacteria occurring at the OFE position, and a significant difference (p < 0.05) being found between the control and the other four groups at the genus level of g_norank_f_norank_o_Actinomarinales. Organic fertilizer had a significant effect on the bacterial Simpson diversity index, with the most significant increasing trend found in OFE (the front eaves of the photovoltaic panel in fertilization area). The results of a correlation analysis showed that pH, electrical conductivity, and total nitrogen were the main factors affecting the soil bacterial community.

Quality detection of alfalfa hay based on multisource information fusion: A preliminary study

The quality detection of alfalfa hay is crucial for the development of animal husbandry. In this study, a method for quality detection of alfalfa hay based on the fusion of multisource information including near-infrared spectroscopy, image processing techniques, and electronic nose is proposed. After SG convolution smoothing, feature wavelengths were extracted using Competitive Adaptive Re-weighting Scheme and Successive Projections Algorithm from the spectral data. The image data were denoised using adaptive wavelet thresholding, and color and texture features were extracted using color histograms and random forest algorithms, respectively. Electronic nose data using principal component analysis was used for data dimensionality reduction. Support Vector Machine, Extreme Learning Machine, and Multi-Layer Perceptron were employed to establish quality detection models of alfalfa hay based on spectroscopy, image, gas information, and their combination, respectively. Experimental results demonstrate that the fusion of near-infrared spectroscopy, image data, and gas information effectively enhances the classification accuracy of the model. The accuracy of the test set reaches 100%, with root mean square error and determination coefficient values of 0.1728 and 0.9239, respectively, surpassing prediction models established solely on individual information. This study provides new insights into alfalfa hay quality detection.

Diversity and host interaction of the gut microbiota in specific pathogen-free pigs.

Pigs are widely used as animal models in various studies related to humans. The interaction between the gut microbiota and the host has significant effects on the host's health and disease status. However, although there have been many studies investigating the pig gut microbiota, the findings have been inconsistent due to variations in rearing conditions. Interactions between the gut microbiota and host have not been fully explored in pigs. Specific pathogen-free (SPF) pigs are ideal non-primate large animals to study the interactions between the gut microbiota and the host. In this study, we performed high-throughput sequencing analysis of the gut microbiota and the gut tissue transcriptome of six SPF pigs to provide a systematic understanding of the composition, function, and spatial distribution of gut microbiota in SPF pigs. We identified significant differences in microbial diversity and functionality among different gastrointestinal tract sites. Metagenomics data analysis revealed significant differences in alpha diversity and beta diversity of microbiota in different gastrointestinal sites of SPF pigs. Additionally, transcriptomic data indicated significant differences in gene expression as well as KEGG and GO functional enrichment between the small intestine and large intestine. Furthermore, by combining microbial metagenomics and host transcriptomics analyses, specific correlations were found between gut microbiota and host genes. These included a negative correlation between the TCN1 gene and Prevotella dentalis, possibly related to bacterial metabolic pathways involving vitamin B12, and a positive correlation between the BDH1 gene and Roseburia hominis, possibly because both are involved in fatty acid metabolism. These findings lay the groundwork for further exploration of the co-evolution between the microbiota and the host, specifically in relation to nutrition, metabolism, and immunity. In conclusion, we have elucidated the diversity of the gut microbiota in SPF pigs and conducted a detailed investigation into the interactions between the gut microbiota and host gene expression. These results contribute to our understanding of the intricate dynamics between the gut microbiota and the host, offering important references for advancements in life science research, bioproduct production, and sustainable development in animal husbandry.

Production of cleavage-resistant phytase transgenic pigs by handmade cloning

With the rapid development of intensive animal husbandry in the livestock industry, large quantities of manure waste containing phytate phosphorus are being generated. Phytase can effectively solve the problem of high phosphorus pollution in the feces of monogastric animals. Enviropig, which produces phytase in the salivary glands and secretes the enzyme in the saliva, were first generated in 1999. However, phytase is easily inactivated during digestion. To address this problem, cleavage-resistant phytase transgenic pigs were generated using handmade cloning in this study. Transgene construction was improved and three cell lines carrying Cafp were obtained. In total, 810 blastocysts were generated and 712 good-quality were transferred into six recipients. Fourteen piglets were born, of which six survived after weaning. Polymerase chain reaction and sequencing results showed that seven (three live and four dead) of the fourteen piglets carried Cafp. Phytase activity in the saliva of the six live cloned pigs was tested at four months of age, and only one pig had 0.155 FTU/mL enzyme activity. The other five pigs may not have been activated in the transgenic parotid gland. Among all the transgenic pigs, the highest phosphorus digestion rate was 59.2% of intake, representing a 25.4% decrease in fecal emission compared to the average of controls. Immunohistochemical results on the three Cafp-positive pigs that died after six months of age showed that the transgene was only expressed in parotid glands, confirming tissue-specific gene expression. In conclusion, cleavage-resistant phytase transgenic pigs were successfully produced through handmade cloning. The cloned pigs offer a unique biological approach to managing phosphorus nutrition and environmental pollution in animal husbandry.

Exploration of Sustainable Agro-Pastoral Integration Development Models in the Qinghai-Tibet Plateau

The high-altitude geographical characteristics and unique climate types of the Qinghai-Tibet Plateau have dictated an agricultural and pastoral development path for the region, which, after a series of evolutionary developments, has culminated in the current model of agro-pastoral integration. However, the economic and ecological benefits brought by the development of agriculture and animal husbandry are accompanied by serious issues such as land degradation and overgrazing, making the exploration and practice of sustainable agro-pastoral integration models for the Qinghai-Tibet Plateau an urgent task. This article utilizes CiteSpace to review recent literature on agro-pastoral development models in the Qinghai-Tibet Plateau. Starting from the agro-pastoral processes, spatial layout, and economic development of the Qinghai-Tibet Plateau, this paper aims to provide a macroscopic understanding of the agro-pastoral integration model, propose new strategies and ideas for development, and offer scientific theoretical and practical guidance for sustainable development, achieving a win-win situation for economic growth and ecological protection.