Long-span timber floors are susceptible to annoying floor vibrations caused by human activities, which, in many cases, govern the timber floor design. Consequently, a reliable prediction of floor vibration responses under human activities, which relies on appropriate walking load models, can be crucial in the design to keep timber floors remaining competitive in the commercial building market. Much of the current design guidance for timber floor vibrations have been established from short-span floors in a residential context, and as a result, many designers refer to established design methods formulated for use with concrete and steel-framed buildings. These guidelines predict the floor response based on a deterministic single-person walking load model that differs depending on the classification of the floor as either a high- or low-frequency floor, which are assumed as a transient or resonant floor response, respectively. Recent advances in modeling human walking have been made, including a single footfall trace load that avoids the need to classify the floor, as well as load models that incorporate a probabilistic approach. To date, an investigation on different walking load models to predict the vibration response of long-span timber floors has not been undertaken, partially due to the fact that there are limited examples in practice. This paper presents the results of a recently completed state-of-the-art research project involving full-scale testing of long-span timber floors and the development of novel numerical models to investigate the applicability of the deterministic walking load model used in current floor vibration design guides, as well as two innovative single-person walking load models for predicting the floor responses of a single long-span timber cassette floor. The numerical investigation was carried out with a finite-element model calibrated with experimentally obtained modal properties. The comparison between the predicted responses and the measured walking test results leads to the recommendation that a selection of an appropriate load model plays a crucial role in accurately and reliably predicting the floor response of a long-span timber cassette floor. Finally, the research undergirding this paper forms an essential basis for understanding the dynamic behavior of long-span timber floors for use in medium-rise timber buildings.