Determination Of Urinary Hydroxyproline Research Articles

Correlation between Vitamin C Deficiency and Hydroxyproline Amino Acid in Young Children of Northern Part in Palestine

Vitamin C is a water soluble vitamin found in many natural sources, citric fruits, vegetables, particularly as antioxidants and as a factor to catabolism and metabolism. The aim of this article is to correlate the level of vitamin C and the hydroxyproline level in urine samples among young palestinian children from age 5 - 14 years. Materials and Methods: Urine samples of 34 individuals of both sexes, their ages ranged from 5 - 14 years were collected and analyzed for determination of Urinary Hydroxyproline by using Modified Neuman and Logan Method. The result of this study shows a significant correlation between Vitamin C and Hydroxyproline at level 0.01. The conclusion of this study was that low vitamin C intake was found among young palestinian children from age 5 - 14 years, Vitamin C should be supplemented in the drinks taken by young palestinian children from age 5 - 14 years as well as further research and investigation with large samples required to include all children from middle and southern Palestine.

Supralesional and sublesional bone mineral density in spinal cord-injured patients

This study was performed to evaluate supra- and sublesional bone mineral density (BMD) in spinal cord-injured (SCI) patients after 1 year postinjury, and to correlate the BMD to the neurological level; to correlate the sublesional demineralization to functional parameters (duration postinjury, duration of the initial bedrest); and to assess the role of classic methods of prevention such as walking or standing. Thirty-one SCI patients, all male, were studied vs. 31 controls (age matched). The mean age of the population was 36 years (range 18–60 years). Eleven were tetraplegic and 20 were paraplegic. Twenty-six patients dysplayed a complete motor lesion. The BMD was measured by dual-photon absorptiometry on the lumbar spine and on the femoral neck, and the bone mineral content (BMC) on whole-body scans. Particular attention was paid to the distal femur and proximal tibia upper third. Blood samples and urine samples included phosphocalcic parameters, with determination of urinary hydroxyproline and deoxypyridinoline. SCI patients showed a decrease of sublesional BMD of 41% in comparison with controls. This loss of bone mass is higher at the distal femur (−52%) and proximal tibia (−70%), which are the most common sites of fracture. The degree of demineralization for the lumbar spine, the pelvis, and the lower limbs is independent of the neurological level. The duration of acute posttraumatic immobilization (mean 43.3 days) and the time postinjury increase the loss of bone mass for lower limbs ( p = 0.04) and particularly for the proximal tibia ( p = 0.02). The study of biomechanical stress (i.e., standing, walking, sitting) does not influence the sublesional BMC. This study underlines the major role of the neurological lesion on the decrease of sublesional BMC in SCI patients and the absence of influence of biomechanical stress.

The determination of urinary hydroxyproline

Three methods of determining the total hydroxyproline content in urine were compared to check the inhibitory influence of some urinary constituents on the analysis. It is recommended to perform Chromatographic purification through an ion-exchange resin column prior to the determination of hydroxyproline.

Urinary hydroxyproline and calcium metabolism in patients with cancer.

IN the past few years an increasing interest has developed in the determination of urinary hydroxyproline as an index of the metabolism and turnover of collagen. Recent experimental1 , 2 and clinical studies3 4 5 6 7 8 9 10 11 12 13 14 15 16 performed in normal as well as in many endocrine, metabolic and bone diseases have indicated that bone collagen is the major source of this amino acid in urine. The present investigation was undertaken to study the excretion of total urinary hydroxyproline in patients with inoperable or widespread carcinoma, especially those with bone metastases or hypercalcemia or both. Our specific aim was to observe what relation exists between urinary excretion . . .