Determination Of Synthetic Estrogens Research Articles

Determination of synthetic estrogens in milk by a novel hyper-crosslinked polymer SPME coupled with HPLC-MS

A novel hyper-crosslinked 2, 8, 14, 20-tetraphenyl calix [4] resorcinol arene polymer (HCP-TCRA) was synthesized by using an external crosslinking of 2, 8, 14, 20-Tetraphenyl calix [4] resorcinol arene (TCRA) as monomers and dimethoxymethane as crosslinker. The HCP-TCRA was explored as a SPME fiber coating material. A variety of physicochemical characterization demonstrated that the material has excellent chemical/thermal stability and strong hydrophobicity. The fiber demonstrated fantastic adsorptive property for three synthetic estrogens (diethylstilbestrol, hexestrol, ethinyloestradiol, abbreviated to SESs). In this work, an efficient HCP-TCRA fiber-based SPME method was developed to analyze SESs by coupling with HPLC-MS detection. Under the optimized SPME conditions, this method was shown a wide range of linearity (2.0–100.0 ng mL−1 for diethylstilbestrol, 1.0–100.0 ng mL−1 for hexestrol, and 30.0–480.0 ng mL−1 for ethinyloestradiol), low limits of detection (LODs, 0.5 ng mL−1 for DES 0.3 ng mL−1 for HEX, 10.0 ng mL−1 for EES) and fine reusability (more than 30 times). Finally, the proposed methods had been applied to the determination of SESs in milk samples with a simple pretreatment. At three different levels, relative recoveries presented between 86.7 and 106.4%. The method put up highly sensitive and budget-friendlyin detection SESs, and has capacity in quality control of milk products.

Determination of Synthetic Estrogens in Milk by a Novel Hyper-Crosslinked Polymer Spme Coupled with Hplc-Ms

Determination of Synthetic Estrogens in Milk by a Novel Hyper-Crosslinked Polymer Spme Coupled with Hplc-Ms

Magnetic molecularly imprinted polymers synthesized by surface‐initiated reversible addition‐fragmentation chain transfer polymerization for the enrichment and determination of synthetic estrogens in aqueous solution

J. Sep. Sci. 2015, 38, 2670–2676 DOI: 10.1002/jssc.201500407 The novel magnetic molecularly imprinted polymers for enrichment of synthetic estrogens were synthesized via surface-initiated reversible addition addition-fragmentation chain transfer polymerization using diethylstilbestrol as template. The uniform imprinted surface layer and the magnetic property of the magnetic molecularly imprinted polymers favored a fast binding kinetics and rapid analysis of target molecules of synthetic estrogens, i.e. diethylstilbestrol, hexestrol, dienestrol, and bisphenol A, with excellent recovery and detection limit. It provides a versatile strategy for the preparation of well-defined solid-phase extraction materials for the analysis and extraction of synthetic estrogens in water.

Magnetic molecularly imprinted polymers synthesized by surface-initiated reversible addition-fragmentation chain transfer polymerization for the enrichment and determination of synthetic estrogens in aqueous solution.

Magnetic molecularly imprinted polymers have attracted significant interest because of their multifunctionality of selective recognition of target molecules and rapid magnetic response. In this contribution, magnetic molecularly imprinted polymers were synthesized via surface-initiated reversible addition addition-fragmentation chain transfer polymerization using diethylstilbestrol as the template for the enrichment of synthetic estrogens. The uniform imprinted surface layer and the magnetic property of the magnetic molecularly imprinted polymers favored a fast binding kinetics and rapid analysis of target molecules. The static and selective binding experiments demonstrated a desirable adsorption capacity and good selectivity of the magnetic molecularly imprinted polymers in comparison to magnetic non-molecularly imprinted polymers. Accordingly, a corresponding analytical method was developed in which magnetic molecularly imprinted polymers were employed as magnetic solid-phase extraction materials for the concentration and determination of four synthetic estrogens (diethylstilbestrol, hexestrol, dienestrol, and bisphenol A) in fish pond water. The recoveries of these synthetic estrogens in spiked fish pond water samples ranged from 61.2 to 99.1% with a relative standard deviation of lower than 6.3%. This study provides a versatile approach to prepare well-defined magnetic molecularly imprinted polymers sorbents for the analysis of synthetic estrogens in water solution.

Determination of Synthetic Estrogens in Illegal Veterinary Formulations by HPTLC and HPLC

To determine the actual amount of diethylstilbestrol, hexestrol, and dienestrol in formulations such as pellets and oily injections that are illegally available on the Brazilian market, a simple methanol extraction is used for the analysis of the pellets and an ether extraction with Sephadex columns (for clean-up) is used for the oily injections. High-performance thin-layer chromatography is used for identification (as a qualitative and semiquantitative method), and high-performance liquid chromatography is used for quantitation. The results of the analysis show that all the formulations are not in accordance with the information listed on their labels.