Determination Of Stigmasterol Research Articles

Development and validation of RP-HPLC method for the determination of stigmasterol in the botanical extract of Ficus deltoidea

A simple, rapid, accurate and precise RP-HPLC method was developed for the determination of stigmasterol in botanical extract of Ficus deltoidea. Separation was achieved with acetonitrile and acetic acid in water (75:25% v/v) in isocratic mode at 210 nm. Single sharp peak of standard stigmasterol was detected at retention time 3.17 min which overlay with the peak of plant extract at 3.14 min. The calibration curve was found to be linear in a concentration range of 2–10 μg/ml with correlation coefficient of 0.998. The LOD and LOQ were found to be 1.50 μg/ml and 4.55 μg/ml respectively. Accuracy and precision was determined with overall recovery of 99.6–100.1% for stigmasterol and RSD values in both intra-day and inter-day repeatability assay lesser than 0.340%, respectively. The robustness study also indicated that there is no influence of minor changes in detecting wavelength and flow rate of mobile phase on the response.

IDENTIFICATION, QUANTIFICATION AND VALIDATION OF STIGMASTEROL FROM ALPINIA CALCARATA USING HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHY METHOD

Objective: The present study is designed to develop a new simple, precise, rapid and selective high‐performance thin‐layer chromatographic (HPTLC) method for the determination of stigmasterol in methanolic rhizomes extract of Alpinia calcarata.Methods: As per International Conference on Harmonization (ICH) guidelines we have applied different concentrations of stigmasterol as standard on HPTLC plates for the quantification of stigmasterol from the Alpinia calcarata rhizomes. The concentration of standard stigmasterol is 1 mg/ml.Results: The retention factor of stigmasterol was 0.58. Linearity was obtained in the range of 50 ng‐250 ng for stigmasterol. The developed and validated HPTLC method was employed for stigmasterol in methanolic rhizomes extract of Alpinia calcarata for standardization of the content of the marker. The linear regression data for the calibration plots showed a good linear relationship with r=0.99977 for stigmasterol, respectively Satisfactory recoveries of 99.77 % were obtained for stigmasterol.Conclusion: The results obtained in validation assays indicate the accuracy and reliability of the developed HPTLC method for the quantification of stigmasterol in methanolic rhizomes extract of Alpinia calcarata

Determination of Stigmasterol in Jatropha Seed Oil by High Performance Liquid Chromatography

A simple and rapid high performance liquid chromatography (HPLC) assay was developed to identify and measure the stigmasterol in jatropha seed oil. The stigmasterol was isolated with a good selectivity by HPLC employing reversed phase C18 columns. The components were separated by mobile phase of methanol-water (99/1, v/v) and detected at 202nm. The quantitation of the stigmasterol was reproducible and the method relative standard deviation is 1.6%. The mean analytical recovery was 97.0%．

HPTLC determination of stigmasterol and tocopherol acetate inLeptadenia reticulataand in its formulation

Leptadenia reticulata W and A (Jivanti) is a shrub of the Asclepiadaceae family with many medicinal uses. Two high-performance thin-layer chromatographic methods have been developed for separate quantitative analysis of stigmasterol and dl -α-tocopherol acetate, two marker compounds in Leptadenia reticulata . The methods are rapid, simple and accurate and can be used for routine quality testing.

Determination of stigmasterol, β-sitosterol and stigmastanol in oral dosage forms using high performance liquid chromatography with evaporative light scattering detection

A validated and repeatable high performance liquid chromatography (HPLC) method with online evaporative light scattering (ELSD) was developed for the analysis of two sterols, stigmasterol, β-sitosterol and a stanol, stigmastanol, found to be common in many herbal formulations and health care supplements. The method is based on the separation of the three marker compounds on a C 8 column (Phenomenex Luna, 5 μm, 150 mm × 4.6 mm i.d.) using methanol:water (95:5 v/v) as the mobile phase, and a flow rate of 1 ml/min to separate all the marker compounds within 12 min. Cholesterol (50 μg/ml) was used as internal standard and methanol as the extraction solvent. The ELSD response parameters were optimised and the limits of detection (LOD) and quantification (LOQ) were calculated to be 2 and 5 μg/ml, respectively, which is more sensitive than obtained by photo diode array detection (5 and 7 μg/ml). Using ELSD, the percentage relative standard deviation (%R.S.D.) of intra-day and inter-day (3 days) precision for each marker was better than 3%, the accuracy data were within 97–103% and the recovery data were found to be within 95–107% for the five commercially available products examined. This method was used to assay commercially available products formulated as oral dosage forms purported to contain African Potato and associated sterols and stanol and proved to be suitable for the routine analysis and quality control of such products.

Determination of Stigmasterol in Soybean Sterol Mixtures by Infrared Method

Determination of Stigmasterol in Soybean Sterol Mixtures by Infrared Method

Determination of Stigmasterol in Soy Sterois by Radioactive Isotope Dilution

Determination of Stigmasterol in Soy Sterois by Radioactive Isotope Dilution