Determination Of Nitrilotriacetic Acid Research Articles

Determination of NTA and EDTA in authentic water samples by LC-MS/MS combined with precolumn methylation

Nitrilotriacetic acid (NTA) and ethylenediaminetetraacetic acid (EDTA), as widely used chelating agents, have created many environmental issues and raised much attention regarding their environmental occurrence. However, the determination of NTA and EDTA in samples of complex solution chemistry remains challenging. Here, we proposed a simple and sensitive method based on liquid chromatography tandem mass spectrometry (LC-MS/MS) to determine NTA and EDTA from aqueous samples without an enrichment step. During the analysis, H+-cation exchange was used to efficiently eliminate interference from metal ions. The intensity of NTA and EDTA with trimethylsilyldiazomethane methylation could be improved hundreds of times that of the virgin samples. To maximize sensitivity, the sample pretreatment methods, the derivatization procedures and the LC-MS/MS conditions were systematically optimized. The validated method showed low limits of quantification (0.4 μg/L for NTA and 1.5 μg/L for EDTA), a wide linear response (up to several mg/L, R2 > 0.998), and good intra- and inter-day precision (relative standard deviation ≤ 11%). The reliability of this method was successfully validated by analysis of authentic water samples collected from three WWTPs and one river with satisfactory recoveries (80–117%). It can be proposed as a powerful analytical method of NTA and EDTA in authentic water samples and could potentially be adapted to detect other chelates and polydentate compounds.

Determination of Nitrilotriacetic Acid in Waste and Natural Waters

An analytical method and the method validation for the determination of NTA in waste waters and in the aquatic environment is presented. An aqueous sample, after evaporation to dryness, was treated with an ethyl esterification reagent. The resulting ester was identified by MS and determined by GC-NPD apparatus. The response was linear within realistic scale (0.006 mg/l—50 mg/l) for lake and waste waters. The recoveries were found to vary between 105 and 123 % for lake water and between 113 and 124 % for waste water. The run-to-run repeatabilities and reproducibilities were all below 2.1 %. revealing the high precision of the method. The detection limit was 6 μg/l and quantitation limit 13 μg/l. No interferences were observed.

Determination of NTA and EDTA and Speciation of Their Metal Complexes in Aqueous Solution by Capillary Electrophoresis

The interest in the use of chelates for enhancing metal uptake by plants during phytoremediation and their widespread use in plant nutrient research requires that an easy, reproducible method be developed for chelate detection in a variety of systems. This work examined the use of capillary electrophoresis for chelate analysis. Electropherograms for nitrilotriacetic acid (NTA) and ethylenediaminetetraacetic acid (EDTA) and their metal complexes were obtained by capillary electrophoresis in a phosphate buffer by direct UV detection at 185 nm. The metals used were Ca(II), Co(II), Ni(II), Cu(II), Zn(II), Cd(II), Pb(II), and Fe(III). For the majority of metals studied, linear calibration curves were obtained in the concentration range of 10−1000 μM. The direct method of determination at 185 nm was found to give a higher detection sensitivity than direct detection at 254 nm. Limits of detection for the metal chelate complexes were in the range of 2−50 μM. The analysis was fast (<6 min), exhibited low relative ...

Determination of nitrilotriacetic acid and ethylenediaminetetraacetic acid in environmental samples as their methyl ester derivatives by gas chromatography-mass spectrometry

A method for the determination of nitrolotriacetic acid (NTA) and ethylenediaminetetraacetic acid (EDTA) in environmental samples such as river water and sediment is reported. An aqueous sample, after evaporation to dryness, was treated with a boron trifluoride-methanol mixture. The resulting methyl ester derivatives were determined by capillary GC-MS with selected-ion monitoring. NTA and EDTA could be determined in the ranges 4.1–12.2 and 3.9–11.8 ng/ml in water with relative standard deviations (R.S.D.s) of 3.1–7.8% and 11.0–19.7%, respectively. Their recoveries from river water and sediment were 74–92% with R.S.D. 1.5–6.8% and 51–60% with R.S.D. 14–16%, respectively.

Liquid chromatographie determination of ethylenediaminetetraacetic acid as metal complexes on a porous graphitic carbon column

A novel liquid Chromatographic system for determination of ethylenediaminetetraacetic acid (EDTA), using a complex-forming metal ion [Fe(III)] in the eluent was developed. Optimisation of the system was obtained after a careful choice of complex-forming metal and pH of the eluent. The porous graphitic carbon column permits use of a wide pH range and due to its high hydrophobicity it is possible to determine EDTA without addition of ion-pair reagents to the eluent. The Chromatographic behaviour of the Fe(III) complex, the free EDTA and Fe(III) ions were considered and possible pitfalls due to equilibrium disturbances are shown and discussed. Applications to analysis of EDTA in local anaesthetic parental solutions and determination of nitrilotriacetic acid as an impurity in EDTA substance are given.

Determination of nitrilotriacetic acid in water by derivative pulse polarography at a hanging mercury drop electrode

Determination of nitrilotriacetic acid in water by derivative pulse polarography at a hanging mercury drop electrode

Determination of nitrilotriacetic acid in detergent formulations

Determination of nitrilotriacetic acid in detergent formulations

Nltrilotriacetic acid as a chelating agent: Applications, toxicology, and bio‐environmental impact

Abstract The chelating characteristics of nitrilotriacetic acid (NTA) are presented and illustrated with respect to its application in medicine, pharmacy, industry (including detergent formulations), agriculture, and the food industry. The analytical chemistry of NTA is presented with respect to its application as an analytical reagent as well as in the separation and determination of NTA itself. A brief account on the toxicology and bio‐environmental aspects of this chelating agent is also part of this review. Special attention is paid to the controversy generated around NTA and its trisodium salt as a possible substituent for phosphate in heavy‐duty detergent formulations. A table of stability constants for metal complexes of NTA (including mixed‐ligand species) is also presented.

Determination of nitrilotriacetic acid by high-speed ion exchange chromatography.

Determination of nitrilotriacetic acid by high-speed ion exchange chromatography.

The determination of nitrilotriacetic acid in granular detergent formulations with a Technicon Auto Analyzer

The determination of nitrilotriacetic acid in granular detergent formulations with a Technicon Auto Analyzer

The determination of nitrilotriacetic acid (NTA) in sewage and sewage effluent

Published methods for the determination of NTA have been examined and all have been found to be subject to interference in analysis of sewage and sewage effluents. The inadequacies of the various methods are described. Passage of the sample through a chelating resin column as a pre-treatment to eliminate metal interference is proposed as a modification of the zinc-zincon method of determination. The procedure thus modified has given quantitative recovery of NTA from the samples analysed.

Determination of nitrilotriacetic acid

A specific method for the determination of NTA in sewage is reported. The NTA is first absorbed on a anion exchange resin and is then eluted with hydrochloric acid. The hydrochloric acid solution is evaporated and the NTA is esterified using a solution of boron trifluoride in 2-chloroethanol. The resulting NTA tri-(2 chloroethylester) is determined by means of gas chromatography. The identity of the compound analysed as NTA was proven by combined gas chromatography-mass spectrometry.

Determination of Nitrilotriacetic Acid in Inland Waters by Gas Chromatography of the Trimethyl Ester

A method for the analysis of low concentrations of nitrilotriacetic acid (NTA) in natural waters was developed. The NTA was converted to the trimethyl ester and determined quantitatively by gas chromatography using methyl heptadecanoate as an internal standard. The lower limit of detection was 25 μg/liter of NTA. The identity of the ester was confirmed using a mass spectrometer–gas chromatograph combination.

Determination of Nitrilotriacetic Acid (NTA) with Ion-Selective Membrane Electrodes

Abstract A solid membrane copper ion selective electrode was used for the determination of NTA via complexometric titration with copper(II). Interferences, of the type commonly found in detergents, were investigated.

Determination of nitrilotriacetic acid and ethylenediaminetetra-acetic acid in granular detergent formulations

Determination of nitrilotriacetic acid and ethylenediaminetetra-acetic acid in granular detergent formulations

Polarographic determination of nitrilotriacetic acid in ethylenedinitrilo-tetraacetic acid.

Polarographic determination of nitrilotriacetic acid in ethylenedinitrilo-tetraacetic acid.