Determination Of Niacinamide Research Articles

Overview of the principle of detecting whitening components by high performance liquid chromatography

With the scientific development of chemical technology and life technology, more and more substances have been discovered and applied to current human production and life. In the process of substance analysis and identification, effective detection tools are needed to accurately identify substances. As a rapidly developing and efficient detection method, HPLC has been widely used in the detection of various cosmetic substances, including nicotinamide, firstly introduces the development background of HPLC, the importance of skin whitening in today's society, and some common whitening ingredients and principles. The application of HPLC in the determination of niacinamide, 3-o-ethyl ascorbic acid and α-arbutin is introduced in the following, and the detection principle and advantages and disadvantages of using HPLC for detection are analyzed. Finally, we analyze and summarize the factors that affect HPLC detection. This approach can be used for market research and quality control of functional cosmetics.

Optimization of ultrasound-assisted dispersive liquid–liquid microextraction of niacinamide in pharmaceutical and cosmetic samples using experimental design

In this paper, an efficient and inexpensive analytical procedure using sugar alcohol-based deep eutectic solvent ultrasound-assisted dispersive liquid–liquid microextraction (DES-UA-DLLME) was optimized for the extraction of niacinamide from vitamin supplements, syrups and cosmetic samples. After the DES-UA-DLLME procedure, the niacinamide content in the samples was determined by UV–VIS spectrophotometer at 265 nm. Box-Behnken design was applied for the optimization of important variables that have a positive effect on extraction recovery of niacinamide. The optimum conditions included: DES-4 solution (mixture of sorbitol and glycerol with a molar ratio of 2:1) volume, 335 µL; pH, 6.1; sonication time 1.5 min; and extraction temperature, 30 °C. Using the optimum values, the DES-UA-DLLME procedure provides good linear range (1–400 ng mL−1), low detection limit (0.33 ng mL−1), high enrichment factor (227), quantitative recovery (97.3–98.9%) and good precision (1.4–2.8%). To investigate the validity of the optimized methodology, two standard reference material were analyzed with the DES-UA-DLLME-UV–VIS method. No significant difference was observed between the measured values and the reference values. The DES-UA-DLLME procedure can be successfully applied in routine analysis for extraction and determination of niacinamide in vitamin supplements, syrups and cosmetic samples.

Determination of Niacinamide in Lotions and Creams Using Liquid–Liquid Extraction and High-Performance Liquid Chromatography

Chemical separations are an important part of an undergraduate chemistry curriculum. Sophomore students often get experience with liquid–liquid extraction in organic chemistry classes, but liquid–liquid extraction is not as often introduced as a quantitative sample preparation method in honors general chemistry or quantitative analysis classes. This experiment offers a relevant, interesting way to use liquid–liquid extraction and high-performance liquid chromatography (HPLC) to analyze popular lotions and creams for niacinamide content. After completing the experiment, students will have a better understanding of the extraction technique, Beer–Lambert law, and HPLC. The percent recovery from a spiked sample of Olay Original Active Hydrating Beauty Fluid was found to be 99.22% with a standard deviation of 0.01%.

Use of column switching for the determination of niacinamide in compound feed

A high-performance liquid chromatographic method is described for the determination of niacinamide in compound feed. The niacinamide is extracted with 0.2 M hydrochloric acid in order to suppress the hydrolysis of the niacinamide to nicotinic acid. The chromatography is carried out with the aid of column switching. An RP-18 column is used for the preseparation, and the analytical separation takes place in a cation-exchange column. With the proposed method, the limit of determination is about 2 ppm of niacinamide.

Polarographic Determination of Niacinamide in Multivitamin Preparations

Abstract Niacinamide is extracted from multivitamin preparations with sodium bicarbonate solution. The extract is mixed with Celite and packed into a chromatographic column. The vitamin is eluted with 10% re-propanol in ether and the eluate is evaporated to dryness in the presence of hydrochloric acid. The residue is dissolved in potassium chloride solution and diluted to volume. The polarogram, from –1.40 to –1.90 v, is recorded on a polarograph equipped with a dropping mercury electrode and an H-cell with a saturated calomel reference cell. Additional assays with chemical and microbiological methods showed comparable results. The method is sensitive to 2 mg niacinamide.

Determination of Niacinamide in Pharmaceutical Preparations

A method for the isolation of niacinamide from multi-component pharmaceutical preparations by partition chromatography between a diatomaceous earth-water column and chloroform followed by measurement of UV absorbance at 262 mμ is proposed. A second method using the color produced by the niacinamide in the column eluate with bromothymol blue in chloroform is also proposed to allow measurements in the visible range. The chromatographic separation is specific for niacinamide and the procedures are simple and rapid. However, alcohol modifies the partition between water and chloroform, causing the niacinamide to be eluted early. The SD of the UV absorbance method is 0.5% and of the bromothymol blue method is 1.7%. The precision of the proposed UV absorbance method compares favorably with that of the Pelletier and Campbell method and gives values approximately 2% higher.

Polarographic determination of ascorbic acid, riboflavin, pteroylglutamic acid, 2-methyl-1,4-naphthohydroquinone diacetate, thiamine, niacinamide and thiamine propyl disulfide in multivitamin preparations.

Ascorbic acid, riboflavin and pteroylglutamic acid in multi-vitamin preparations can be concurrently determined by polarography in phosphate buffer solution (pH 6.8). The first is represented by its anodic wave at E1/2-0.05V and the next two by their cathodic waves at E1/2-0.47 and -1.2V vs NCE, respectively. The reduction wave at E1/2 -0.41V in 0.1N HCl solution is also utilized for the determination of pteroylglutamic acid in the mixture. 2-Methyl-1, 4-naphthohydroquinone diacetate (K) is determined by means of the redox wave of menadione which is formed by alkali hydrolysis of K. Thiamine in such preparations is determined by the maximum wave which is observed at ca. -1.5V in its solution containing 1mM CoCl2, 0.1M NH4Cl and 0.1M NH3.The determination of niacinamide is effected by means of its cathodic wave at -1.8V in 0.1N NaOH. Thiamine propyl disulfide (TPD) in a mixture of TPD, thiamine, riboflavin and “Tween” is determined by means of the reduction wave at E1/2 -0.7V in pH 4 acetate buffer, in which condition riboflavin is also determined by its wave at E1/2 -0.34V. Thiamine in such a mixture is determined by means of its anodic wave at E1/2 -0.44V in pH 10-12 phosphate buffer.