Determination Of Isoniazid In Tablets Research Articles

A Redox-induced Dual-mode Colorimetric and Fluorometric Method based on N-CDs and MnO2 for Determination of Isoniazid in Tablets and Plasma Samples.

We develop a simple hydrothermal method to prepare a novel nitrogen-doped carbon dots (N-CDs) originated from green carbon source Liu-bao tea and ethylene diamine. The N-CDs emits strong and stable blue fluorescence (Em=440nm) under the excitation wavelength of 350nm with a quantum yield of 35%. And it is used as an excellent fluorescent output for the sensitive and visual dual-mode determination of isoniazid. The fluorescence of N-CDs is "turned off" first by manganese dioxide (MnO2) nanosheets due to inner filter effect, MnO2 nanosheets can also oxidize TMB (3,3',5,5'- tetramethylbenzidine) to blue oxTMB. Isoniazid, however, can reduce MnO2 nanosheets to Mn2+, turning on the fluorescence again. The color of the solution fades from blue to colorless because less TMB can be oxidized. Under the optimal conditions, the dual-mode method has a satisfying linear relationship ranging from 2.0 to 120.0μM with a limit of detection of 0.7μM (S/N=3). And it has been applied successfully to colorimetric and fluorescent determination of isoniazid in tablets and clinical plasma samples, with recoveries ranging from 94.0% to 102.4%. The properties of N-CDs and MnO2 nanosheets were thoroughly characterized using TEM, FT-IR, XPS, AFM and fluorescence spectrophotometer, the quenching mechanism was also discussed.

An electrochemical sensor based on MOF-derived NiO@ZnO hollow microspheres for isoniazid determination.

The electrocatalytic activity of metal-organic framework (MOF)-derived NiO@ZnO hollow microspheres was studied for its application to an isoniazid sensor. The MOF-derived NiO@ZnO hollow spheres were synthesized by the coordination reaction of terephthalic acid with Zn2+ and Ni2+ and followed by calcination. Morphology characterization showed that the MOF-derived NiO@ZnO sphere has circular core-shell structure with pores on its surface. Further electrochemical characterization of the prepared sensor by cyclic voltammetry and differential pulse voltammetry proved that the material has good electrical conductivity and strong catalytic ability. Distinct oxidation peaks occur for INZ at potential of 0.22V (vs. saturated calomel electrode). Under the optimal experimental conditions, the linear range of the sensor for isoniazid determination was 0.8 ~ 800μM, and the detection limit was 0.25μM (S/N = 3). In addition, the sensor displayed good stability, repeatability, and reproducibility. The established method was successfully applied for determination of isoniazid in tablets and mouse serum with admirable accuracy and reliability. Graphical abstract Schematic presentation of an electrochemical sensor based on MOF-derived NiO@ZnO hollow microspheres for isoniazid determination.

Spectrophotometric determination of isoniazid in excess p-aminosalicylate

Abstract The presence of a large excess of p-aminosalicylic acid prevents the direct application of Vierordt’s spectrophotometric method to the determination of isoniazid in tablets. The method can, however, be applied after the bulk of the p-aminosalicylic acid has been removed with ether. The extinction of the aqueous phase containing all the isoniazid and some of the p-aminosalicylic acid is then determined at two wavelengths and the concentration of isoniazid calculated therefrom.

Spectrophotometric Study of Isoniazid by Using 1,2-Naphthoquinone-4-Sulfonic Acid Sodium as the Chemical Derivative Chromogenic Reagent

A new method for the determination of isoniazid by using 1,2-naphthoquinone-4-sulfonic sodium as the chemical derivative chromogenic reagent is established. The method is based on a condensation reaction to measure the pink compound produced by the reaction of isoniazid with 1,2-naphthoquinone-4-sulfonic sodium in pH 13.00 buffer solution. The stoichiometric ratio of the compound is 1:1, and its maximum absorption wavelength is at 460 nm, ϵ = 1.18 × 104 L·mol−1 cm−1. Beer's law is perfectly obeyed in the range of 0.50˜30 μg.mL−1 of isoniazid. The linear regression equation is A = 0.0185 + 0.11056C (mol.L−1), with 0.9994 of a linear regression correlation. The detection limit is 0.40 μg.mL−1, RSD is 0.48%, and average recovery is over 99.3%. This paper further optimizes the determination of isoniazid compared to the previous methods, and the kinetic property and reaction mechanism are studied intensively. This proposed method has been successfully applied to the determination of isoniazid in tablets of isoniazid with satisfactory results.

Determination of isoniazid in tablets by second-derivative ultraviolet spectrophotometry of scraped-spot solutions from thin-layer chromatography

Isoniazid in solutions of tablets was separated by thin-layer chromatography (TLC). The portion of the precoated plate carrying the isoniazid spot was cut out and the coating was scraped off into water. The isoniazid was quantified in the solution, without separation of suspended adsorbent, by second-derivative spectrophotometry, which eliminated the background in the zero-order spectra. Relative standard deviations ( n = 5) were <1%. Results obtained for commercial tablets were in good agreement with those given by a liquid-chromatographic method.