Determination Of Histamine In Fish Research Articles

Colorimetric aptasensor based on Fe3O4@MOF@Pt nanozymes for ultrasensitive detection of histamine in fish

The accurate determination of histamine in fish is imperative for food safety, given the toxic nature of this biogenic amine. This study presents an innovative colorimetric aptasensor for the ultrasensitive detection of histamine in fish, utilizing nanozymes based on magnetic metal-organic frameworks coated with Pt nanoparticles (Fe3O4@MOF@Pt), which exhibit high peroxidase-like catalytic activity, magnetism, and superior optical properties. The surface of Fe3O4@MOF@Pt nanozymes were modified with DNA strands complementary to the histamine aptamer, creating effective colorimetric probes. In the presence of histamine, these colorimetric probes compete with histamine molecules for aptamer binding on the enzyme plate. Color development is triggered by adding a TMB and H2O2 substrate solution to the enzyme plate. This competitive binding-based aptasensor allows for rapid and accurate histamine quantification within a linear range of 10 pM to 10 μM, and a notable detection limit of 6.23 pM. Furthermore, the sensor demonstrated excellent specificity, distinguishing histamine from l-histidine and other biogenic amines. Tested on mackerel samples, the aptasensor showed spiked recoveries of 91.4%–109.6%, validating its practical applicability. This sensor offers an ultrasensitive, specific, and reusable tool for histamine detection, representing a significant advancement in ensuring the safety of fish products.

A simple colorimetric histamine sensor based on smartphone digital image processing for fish quality assessment

Histamine (HA) is commonly employed as a marker for assessing fish spoilage. Digital image colorimetry (DIC) on a smartphone was developed to monitor HA in fish. A synthesized polyvinyl alcohol (PVA) sol-gel entrapped 2,3-naphthalenedicarboxaldehyde (NDA) that produced a blue product in the presence of HA. To fabricate the sensor, the PVA@NDA sol-gel was placed in a microtube that received sample solutions extracted from fish tissue. The intensity of the blue color produced in the presence of HA was correlated with the concentration of HA, which could be semi-quantitatively determined with the naked eye. Smartphone-enabled DIC was applied to digital images of the reaction, using the Color Lab® application to analyze the red channel of the images. Under optimum conditions, the developed sensor exhibited linearity in the range of 6.0 – 75.0 ppm of HA with a limit of detection of 0.31 ppm. Excellent stability, reproducibility, and selectivity were obtained. The developed sensor was successfully applied to detect HA in fish samples, with recoveries of 86 ± 6 – 116 ± 10%. The obtained results from HA determination in fish with the proposed method were consistent with results from the enzyme-linked immunosorbent assay. Therefore, the proposed sensor could be used to ensure the freshness of fish through on-site monitoring of HA.

Development of cloth-based microfluidic devices for rapid determination of histamine in fish and fishery products.

A cloth-based analytical device combined with electrochemiluminescence detection (CAD-ECL) was described for rapid determination of histamine (HA). The CAD device was produced by screen-printing a conductive carbon ink onto a patterned hydrophobic electrochemical microfluidic chamber to fabricate the three-carbon electrode system on a single hydrophilic cloth. The introduction of carbon nanodots linked to chitosan on the working carbon electrode surface enhanced the catalytic performance and overcame the resistance of thecotton fibermaterial. On this basis, the enhancement of the electrochemiluminescence (ECL) signal ofthe tris(2,2'-bipyridyl) ruthenium(II) complex, caused by HA, was observed in a phosphate buffer solution at pH 7.6. The proposed CAD-ECL sensor was successfully applied tothe quantification of HA in fish and fishery samples with good linearity between ECL intensity and the logarithm of HA concentration in the range 1.0 to 1000.0µg L-1 with a low detection limit of 0.82µg L-1.

Разработка методики определения гистамина в рыбе и рыбных продуктах с применением высокоэффективной жидкостной хроматографии с масс-спектрометрическим детектированием

Histamine is a substance dangerous to human health, which may be present in food products in case of violations of storage or production conditions, which may depend both on the content of the initial component (amino acid histidine) in fish, and on the content of decarboxylating microorganisms and the availability of optimal conditions for their reproduction and vital activity. Based on the high danger of histamine for human health, its content is regulated by regulatory documents in many countries of the world, including the Russian Federation. Therefore, it is necessary to carry out strict control of fish and fish products for histamine content. To control the histamine content, modern techniques based on accurate and sensitive analysis methods are needed, such as the method of high-performance liquid chromatography with mass spectrometric detection (HPLC MS/MS). The article is devoted to the creation of HPLC MS/MS methods for the determination of histamine in fish and fish products. The obtained data for chromatographic histamine isolation and its mass spectrometric determination, as well as metrological characteristics of the technique are presented. The expansion of monitoring studies of histamine content in fish and fish products will allow monitoring not only the safety, but also the freshness of products. The article is devoted to the development of HPLC MS/MS methods for the determination of histamine in fish and fish products. The data for chromatographic histamine isolation and its mass spectrometric determination were obtained, the sample preparation algorithm was determined, as well as the metrological characteristics of the technique.

Modification of BIOFISH 300 HIS for Determination of Histamine in Fish and Fish Products: AOAC Performance Tested MethodSM 051604.

The BIOFISH 300 HIS method for the quantification of histamine in fish and fish products was validated by the AOAC Research Institute and granted Performance Tested MethodSM certification in 2016. The method is based on the use of an electrochemical reader (BIOFISH 300 device) together with disposable gold electrodes (Biotest) modified with a specific enzyme that oxidizes the histamine molecules and produces a detectable and quantifiable electric current signal. It was validated for raw fish (tuna, mackerel, sardine, and anchovy), cooked fish (tuna), canned fish (tuna in water, tuna in oil, mackerel in tomato sauce, and pickled sardine), and salted fish (canned salted anchovy). The validated ranges were 30-150 mg/kg histamine for canned salted anchovy and 10-200 mg/kg histamine for all other matrices. The objective of the present report is to validate some method modifications, namely the use of a new reader (BIOFISH 3000), the inclusion of new quantification ranges, new matrixes in the certified claims, a histamine reference solution (Verifying Solution) that will enable the user to perform a system verification, and also the inclusion of the kit ASC1-10 for the elimination of ascorbic acid from sample extract. New quantification ranges can be obtained by diluting the sample appropriately into the measurement cuvette, thereby adjusting the concentration to the internal calibration range of the device. New matrixes such as fish meal, preserved salted anchovy, and fish with added ascorbic acid were validated. The new digitalized reader enables new functionalities such as the use of an intuitive app and cloud storage of the results, with no changes in the data acquisition and processing. As the use of the new reader does not imply any change at the electrochemical readout level, all assays were performed using the BIOFISH 3000 device. The method was shown to be linear in all the quantitation ranges configured. Accuracy and recovery studies over a wide range of matrixes showed that the method yields comparable results to the reference method used. Stability and product consistency testing of kit components demonstrated that the system produces accurate results when expiration dates from the manufacturer are met. All modifications made to the BIOFISH 300 HIS method, PTM 051604, have been validated satisfactorily, under criteria of the AOAC Research Institute. The use of the BIOFISH 300/3000 HIS method is ideal as a routine histamine control method for the fish industry. After a simple aqueous extraction of the sample, results are obtained in less than 3 minutes. All data generated by the new BIO3000 biosensor are sent to a web platform, allowing new functionalities to the user. All these technical capabilities represent a competitive advantage over other existing technologies.

Визначення гістаміну у рибі та рибопродуктах: валідація фотометричного методу

Topicality: The content of histamine in high concentrations causes a number of diseases that even lead to death, it is one of the indicators that characterizes the safety of fish and fish products. Therefore, the choice or development of a simple and inexpensive method for the determination of histamine content in fish and fish products is relevant. Objective: We confirmed the effectiveness of photometric method for the determination of histamine in fish and fish products by obtaining and analyzing validation characteristics. Results: Based on the results of validation, it has been substantiated and experimentally proved that the photometric method of quantitative determination of histamine can be correctly reproduced and is suitable for the quality control of measurements. The most acceptable results were obtained for histamine concentrations in the range from 50.0 ± 0.1 to 200.0 ± 0.5 mg/kg. The lower histamine concentrations are characterized by a higher percentage of total uncertainty, although it is also within acceptable values. Conclusions: Evaluation of the obtained validation characteristics indicates that this technique can be used to quantify the content of histamine in fish and fish products and is not inferior in accuracy and correctness to the method of high performance liquid chromatography.

Influence of Sample Matrix on Determination of Histamine in Fish by Surface Enhanced Raman Spectroscopy Coupled with Chemometric Modelling

Histamine fish poisoning is a foodborne illness caused by the consumption of fish products with high histamine content. Although intoxication mechanisms and control strategies are well known, it remains by far the most common cause of seafood-related health problems. Since conventional methods for histamine testing are difficult to implement in high-throughput quality control laboratories, simple and rapid methods for histamine testing are needed to ensure the safety of seafood products in global trade. In this work, the previously developed SERS method for the determination of histamine was tested to determine the influence of matrix effect on the performance of the method and to investigate the ability of different chemometric tools to overcome matrix effect issues. Experiments were performed on bluefin tuna (Thunnus thynnus) and bonito (Sarda sarda) samples exposed to varying levels of microbial activity. Spectral analysis confirmed the significant effect of sample matrix, related to different fish species, as well as the extent of microbial activity on the predictive ability of PLSR models with R2 of best model ranging from 0.722–0.945. Models obtained by ANN processing of factors derived by PCA from the raw spectra of the samples showed excellent prediction of histamine, regardless of fish species and extent of microbial activity (R2 of validation > 0.99).

Development of the High Sensitivity and Selectivity Method for the Determination of Histamine in Fish and Fish Sauce from Vietnam by UPLC-MS/MS.

A selective, sensitive, and rapid method by using ultraperformance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) for the determination of histamine in fish and fish sauce was developed. The optimal conditions of liquid chromatographic separation and mass spectroscopy of histamine have also been investigated. The linear ranges of the method were 20.0 ÷ 1000 ng/mL, and the corresponding correlation coefficient was 0.9993. Mean recoveries of the analyte at three spike levels (low, medium, and high) were within the range of 98.5% ÷ 102.5% (n = 7). The limit of detection (LOD) and limit of quantification (LOQ) values were 3.83 and 11.50 ng/mL for the fish sauce sample and 4.71 and 14.12 ng/mL for the fish sample, respectively. The influence of the matrix effect on the accuracy, repeatability, and recovery of the method was negligible. The recommended method was applied to determine the content of this substance in 21 fish sauce samples and 4 kinds of fish samples, which were collected from Ho Chi Minh City, Vietnam, in 2019.

D-penicillamine modified copper nanoparticles for fluorometric determination of histamine based on aggregation-induced emission.

A fluorometric method for the determination of histamine has been developedbased on aggregation-induced emission (AIE) effect of D-penicillamine capped copper nanoparticles (DPA-CuNPs). The fluorescent DPA-CuNPs were synthesized by a one-pot method using D-penicillamine as both reducing agent and stabilizing ligand. The size, morphology and physical chemical properties of DPA-CuNPs were examined by transmission electron microscopy (TEM), fluorescence spectroscopy, fourier transform infrared spectroscopy (FTIR) and absorption spectroscopy. The DPA-CuNPs exhibit AIE effect and show intense red fluorescence (650nm). In the presence of histamine, DPA-CuNPs are dispersed into small homogeneous particles, causing fluorescence quenching. Based on this reaction, a histamine sensor is constructed. The fluorescence of the CuNPs solution has a good linear relationship with histamine concentration in the range 0.05μM to 5μM and the determination limit (3σ/slope) is 30nM. The estimated method was successfully applied to the determination of histamine in fish, pork and red wine. Graphical abstract Schematic representation of copper nanoparticles for histamine analysis. In the presence of histamine, the strong red fluorescence of copper nanoparticles is obvious decreased through interaction of copper nanoparticles and histamine.

Disposable biogenic amine biosensors for histamine determination in fish.

This study presents the development of disposable biosensors employed in the determination of histamine in fish samples. Screen printed carbon electrodes (SPCEs) were first modified with a mixture of titanium dioxide nanoparticles (TiO2), carboxylated multiwalled carbon nanotubes (c-MWCNTs), hexaammineruthenium(iii) chloride (RU) and chitosan (CS). Diamine oxidase (DAO) or monoamine oxidase (MAO) enzymes were further immobilized onto the TiO2-c-MWCNT-RU-CS/SPCEs via 1-ethyl-3-(dimethylaminopropyl) carbodiimide hydrochloride (EDC) and hydroxysuccinimide (NHS) chemistry for the fabrication of the biosensors. The morphological and electrochemical properties of the proposed biosensors were studied using scanning electron microscopy (SEM), energy dispersive X-ray (EDX) spectroscopy, cyclic voltammetry (CV), chronoamperometry and electrochemical impedance spectroscopy (EIS). A performance comparison of two biosensors indicated that the one based on DAO had a linear concentration range from 9.9 × 10-6 to 1.1 × 10-3 M and the other based on MAO, from 5.6 × 10-5 to 1.1 × 10-3 M for histamine. The sensitivity of the DAO based biosensor was almost 1.5 times higher than that of the MAO based biosensor. The proposed biosensors were successfully employed to determine histamine in fish samples and the recoveries were between 100.0% and 104.6%.

Determination of histamine in fish without derivatization by indirect reverse phase-HPLC method

Histamine (HA) is an important biochemical for human health and food quality. It is produced due to the decarboxylation of histidine. High level of HA was reported in a large number of fish tissues in Scombridae family. The presence of a low concentration of histamine is the indicator of the freshness of fish. Therefore, this study aims to develop a simple novel method for the quantification of histamine by revers-phase high-performance liquid chromatography (RP-HPLC-UV) followed by conventional UV detector at the wavelength of 205 nm. The extraction of the fish sample was optimized without derivatization, 2-5 dihydroxybenzoic acid (2-5 DHBA) and acetonitrile (ACN) was used in the mobile phase for the first time. Here we thoroughly investigate key experimental conditions such as UV absorbing probe, pH and mobile phase. We found that concentration of 6 mmol/L of 2,5-DHBA in the mobile phase, pH 2.65 and 50:50% ratios of 2,5-dihydroxybenzoic acid and acetonitrile were the suitable conditions for determination of histamine in the fish sample. By applying our new method, we determine the HA concentration within 5 min. This novel method was validated by using different parameters like linearity, limit of detection (LOD), limit of quantification (LOQ), repeatability, matric effect, specificity, trueness, ruggedness, accuracy, precision, and specificity. The LOD and LOQ were 1 and 3 mg/kg respectively, and the correlation coefficient was >0.9995. We applied this method to the fish sample. Which were obtained from a local market here in Shanghai. Recovery was determined after spiking the fish sample; good recovery was observed with average recovery >82%. Thus, the proposed method provides a better reference for the successful analysis of fish safety, quality and trade.

Rapid determination of histamine in fish by thin-layer chromatography-image analysis method using diazotized visualization reagent prepared withp-nitroaniline

A novel analytical method for rapid determination of histamine in fish involves methanol extraction, manual spotting, thin-layer chromatography (TLC) for less than 15 min, visualization by diazotizedp-nitroaniline reagent, and quantitation by image analysis using the ImageJ software.

Determination of histamine in fish by Surface Enhanced Raman Spectroscopy using silver colloid SERS substrates.

This study was focused on development of a rapid and sensitive method for histamine determination in fish based on Surface Enhanced Raman Spectroscopy (SERS) using simple and widely available silver colloid SERS substrate. Extraction of histamine with 0.4M perchloric acid and purification with 1-butanol significantly shortened sample preparation (30min) and provided clear SERS spectra with characteristic Raman bands of histamine. Principal component analysis effectively distinguished SERS spectra of fish samples with different histamine content. Partial least square (PLS) regression models confirmed reliability of detection and spectral analysis of histamine with SERS. In histamine concentration range 0-200mgkg-1, significant in legislative and fish quality control aspects, PLS regression model based on spectral range 1139.9-1643.7cm-1 showed linear trend with R2pred=0.962, RPD=7.250. Presented protocol for histamine extraction and purification followed by SERS analysis coupled with chemometric approach, enabled development of rapid and inexpensive method for histamine determination in fish.

Modification and Single-Laboratory Validation of AOAC Official Method 977.13 for Histamine in Seafood to Improve Sample Throughput

Histamine is the main causative agent in scombrotoxin fish poisoning, the most frequently reported illness related to fish consumption. The AOAC official method for histamine determination in fish is the fluorometric method AOAC 977.13, which is sensitive and reproducible but somewhat labor intensive and time consuming. We investigated multiple modifications to this method in an attempt to reduce assay time and increase sample throughput while maintaining the performance of the original method. Some of the attempted modifications negatively affected the performance characteristics of the method. However, omitting the heating step during extraction and replacing the cuvette style fluorometer with a microplate reader retained method performance while increasing sample throughput. Therefore, we adopted these modifications and conducted a single-laboratory validation. The recovery, precision (RSD), and LOD of the modified method assessed by the single-laboratory validation ranged from 92 to 105%, 1 to 3%, and 0.2 to 0.5 ppm, respectively, in tuna, mahi-mahi, and Spanish mackerel samples. We conclude that the AOAC 977.13 fluorometric method, modified as described, will improve assay time and sample throughput efficiency cumulatively, as the number of sample units analyzed increases. We anticipate that this modified method could be used by regulatory agencies and other laboratories following successful multilaboratory validation.

Optimization and Validation of Analytical Method for Determination of Histamine in Fish using HPLC-FLD

Optimization and Validation of Analytical Method for Determination of Histamine in Fish using HPLC-FLD

Determination of Histamine in Fish and Fish Products by Tandem Solid-Phase Extraction

A simple and practical method was developed for the determination of histamine in fish and fish products by solid-phase extraction and fluorescence derivatization. Histamine was extracted with trichloroacetic acid. The extract was neutralized and diluted with phosphate buffer (pH 6.8), and cleaned up with a tandem-connected octadecyl silica (ODS) and strong cation exchange silica (SCX) cartridge. After removal of the solvent, histamine was derivatized with fluorescamine and analyzed by ion-paired reversed-phase high-performance liquid chromatography with fluorescence detection. Recovery tests of histamine from six kinds of fish and fish products showed acceptable recovery (83-92%) with low relative standard deviation (less than 5%). This method could be useful for determination of histamine in fish.

A simple and rapid method for the analysis of fish histamine by paper electrophoresis

The described analytical method for histamine determination in fish and seafood consists of sample extraction, adsorption onto a paper disc, application of the paper disc onto electrophoresis paper, electrophoresis for only 10 min, drying, and color developing by Pauly’s reagent. Histamine can be satisfactorily detected and completely separated from histidine, carnosine and other Pauly reagent-positive compounds. This method does not require expensive instrumentation and any tedious pretreatment to eliminate potential interference by other imidazole compounds, such as histidine or carnosine. This method can be used to detect histamine in multiple fish and seafood samples simultaneously that contain as little as 15 p.p.m. histamine (1.5 mg/100 g).

Fluorimetric Determination of Histamine in Fish Using Micellar Media and Fluorescamine as Labelling Reagent

An analytical method based on the use of fluorescamine to produce a fluorescent derivative with histamine and combined with micellar-enhanced fluorescence detection of the formed complex is developed for the sensitive and rapid determination of histamine in fishes. The fluorescence properties of the obtained complex in water and micellar solutions of sodium dodecyl sulfate (SDS), cetyltrimethylammonium chloride (CTAC) and brij-700 are reported. Physicochemical variables influencing the sensitivity of the method (pH, micellar, fluorescamine and NaCl relative concentrations) have been optimized. The stability of the formed complex, as shown by kinetic study, depends on the pH of the solution. Linear calibration curves allowing an effective histamine determination were established with large linear dynamic range (LDR), and low limits of detection (LOD) between 0.5 and 33 ng mL-1, according to the solvent. Application to the analysis of fish samples (sardines) yielded satisfactory results. The method seems to be suitable for environmental fish quality control.

A rapid gas chromatographic method for the determination of histamine in fish and fish products

A gas chromatographic (GC) method, which reduced the time for determination of histamine in fish and fish products to less than 20 min, was demonstrated. Contrary to traditional GC method, histamine in sample was initially extracted with alkaline methanol and injected into a GC column (CP-SIL 19CB) for analysis without derivatization. Internal standard used in this protocol was 1,9-nonanediol. Detection limit for histamine by this method was about 5 μg/g. Standard addition test indicated 98–111% (CV: 2.7–7.8%) of recovery for tuna flesh and 99–102% (CV: 2.7–8.9%) for shrimp meat after adding with authentic compound, suggesting that using direct GC analysis for histamine determination was feasible.

Determination of histamine in fish products by capillary electrophoresis and ion-pair liquid chromatography with diode-array detection

A method for histamine determination in fish and fish products by capillary electrophoresis and ion-pair liquid chromatography has been developed. Histamine was extracted from different fish samples with 10% trichloroacetic acid, and the filtrate was directly applied to capillary electrophoresis apparatus. A preliminary purification through a solid phases extraction cartridge (Sep-pak C18) was necessary for HPLC analysis. Histamine was detected ‘on-line’ at 210 nm by both approaches. HPLC procedure allowed accurate (±2.2%) and repeatable (±1.6%) determination of histamine in fish products at appropriate levels (20–100 mg kg−1) to meet current regulations. © 1999 Society of Chemical Industry