An analytical method was established for the simultaneous determination of glucuronic acid and glucuronolactone using ion-exclusion chromatography. The separation was performed on a Boston HC-B75 H+ column at 25 ℃ by HPLC equipped with an ultraviolet detector at the wavelength of 220 nm. The mobile phase is 5 mmol / L sulfuric acid with a flow rate of 0. 6 mL / min. The quantification was performed by an external standard. The linear ranges for glucuronic acid and glucuronolactone are 10 ~ 1200 mg / L and 5 ~ 600 mg / L( r 0. 9999),respectively. The detection and quantification limits for the two compounds are 0. 1 mg / L and 0. 3 mg / L,respectively. The standard deviations for glucuronic acid and glucuronolactone are 1. 00% and 0. 35%,respectively. The recovery ratios for glucuronic acid and glucuronolactone are 99. 69% and 98. 21%,respectively. This method is convenient,rapid,sensitive and suitable for the simultaneous determination of glucuronic acid and glucuronolactone. Temperature,pH and solvent affected the stability of glucuronic acid.