Determination Of Estriol In Urine Research Articles

Enzyme immunoassay of estriol in pregnancy urine.

We describe an enzyme immunoassay for determination of total estriol in urine. Estriol covalently bound to horseradish peroxidase is used as tracer, and free and bound hormone are separated by precipitation with polyethylene glycol. The method can be used with either acid hydrolysis at 100 degrees C for 30 min or enzyme hydrolysis at 50 degrees C for 40 min; results by the former procedure are about 15% lower than results by the latter. Results were practically identical when we compared the enzyme immunoassay with a radioimmunoassay, using the same antiserum and method of hydrolysis. The day-to-day CV for three different concentrations was 10.7-12.0%, the within-series CV 6.6-8.6%. The additional time required for the enzyme reaction is compensated for by the rapid measurement of light absorbance. Thus this method is faster than radioimmunoassay when more than 25 samples are to be assayed.

Quantitative analysis of trace amounts of estrogenic steroids in pregnancy urine by column liquid-liquid chromatography with ultraviolet detection

The influence of the sample volume on peak height and resolution in elution column chromatography was studied. Graphs which enable the choice of the optimum sample volume are given. The quantitative analysis of estrogens by column liquid–liquid chromatography with ultraviolet detection was investigated. The method has been applied to the rapid quantitative determination of estriol in urine. After preparing the urine sample for chromatography, the quantitative information can be obtained in 20 min with a precision of 5″6 for a concentration of 3 mg l. The noise of the sample-free detector corresponds to 60 ng of estriol.

A method for the determination of estriol in urine during pregnancy

A modification of the method of Beling for the estimation of estriol in pregnancy urine is described. The eluate of the gel filtration procedure is used for the Kober reaction without hydrolysis and purification. The correction procedure eliminates nearly all background colour of the eluate. Stability of the colour, sensitivity, reproducibility, recovery and specificity are satisfactory for estriol determinations in the second and third trimester of pregnancy. The method requires no special instruments, and the working time needed is not long.

Microdetermination of Steroid Estrogens in Urine by Fluorometry.

Summary1. A quantitative method for the determination of estradiol, estrone and estriol in urine, based on the fluorescence of these substances when treated with phosphoric acid, is described. 2. The method is more accurate, sensitive and specific than other known biological or colorimetric tests. 3. The recoveries of estradiol were above 80%; of estrone between 70 and 80%; and of estriol between 50 and 60%. The mean experimental error was ± 10%. 4. The described method permits the determination of estriol in urine in quantities not hitherto detectable.