An accurate determination of the band gap energy is crucial for predicting the photophysical and photochemical properties of investigated materials. In the present work, the dependence of the energy band gap (Eg ) of α-LiIO3 crystals doped with L-arginine and L-nitroarginine amino acids was obtained. The method, based on the simultaneous fitting of many absorption mechanisms to the spectral dependence of the Kubelka-Munk function estimated from diffuse reflection data, as well as Tauc's method, has been applied to determine the energy band gap. In the case of a small dopant concentration, additional electron states appear within the band gap of the crystals. As a result, a broad absorption band appears in the material spectrum. The optical transmittance spectra were recorded using an Agilent Cary 60 spectrophotometer in the spectral range 190–1100 nm.