Determination Of Ellagic Acid Research Articles

The extraction and determination of ellagic acid and resveratrol in blueberry species by HPLC-DAD and LC-MS/MS

The extraction and determination of ellagic acid and resveratrol in blueberry species by HPLC-DAD and LC-MS/MS

Determination of Ellagic Acid, Punicalagin, and Castalagin from Terminalia ferdinandiana (Kakadu plum) by a Validated UHPLC-PDA-MS/MS Methodology

Determination of Ellagic Acid, Punicalagin, and Castalagin from Terminalia ferdinandiana (Kakadu plum) by a Validated UHPLC-PDA-MS/MS Methodology

Determination of ellagic acid in rose matrix by spectrofluorimetry

Ellagic acid is a naturally occurring polyphenol with many verified and alleged bioactivities. This study presents a validated, sensitive and fast spectrofluorimetric method to determine ellagic acid in rose matrix, based on the fluorescence properties of ellagic acid–borax complex in methanolic extract. In the presence of rose matrix, a calibration curve was linear in a wide concentration range from 0.5 nmol/L to 1.0 μmol/L of EA, and the detection and quantification limits of ellagic acid were 34.7 nmol/L and 0.11 μmol/L, respectively. We used the validated method to determine the content of ellagic acid in lyophilized and dried rosehip products available in Poland. The rosehip contains a significant content of ellagic acid (from 32 to 44 μg/g of dried/freeze-dried material). All spectrofluorimetric results were in good agreement with those obtained by liquid chromatography–mass spectrometry with a single quadrupole analyzer used as a reference method.

DEVELOPMENT AND VALIDATION OF HPTLC METHOD FOR ESTIMATION OF ELLAGIC ACID IN ANTIDIABETIC HERBAL FORMULATION

A simple, sensitive, precise, rapid and reliable HPTLC method for the estimation of ellagic acid in marketed herbal formulation, Glucomap tablet was developed. In this method, precoated Silica Gel F254 Plates were used as stationary phase and Toluene: ethyle acetate: formic acid: methanol (3:3:8:2 v/v) as mobile phase. Developed chromatogram was scanned at 280 nm, the wavelength of maximum absorption for ellagic acid. The aptness of developed HPTLC method for estimation of ellagic acid was established by validating it as per the ICH guidelines. The content of ellagic acid in crude drug Terminalia arjuna and polyherbal formulation was also studied. The developed method has been successfully applied for the determination of ellagic acid in polyherbal formulation.

Use of functionalized nanoporous silica for the microextraction by packed sorbent of ellagic acid from fruit juice

A semi-automated microextraction by packed sorbent (MEPS) method was developed for the HPLC determination of ellagic acid (EA) in juice samples. The procedure includes enrichment of the analyte on a nanoporous silica sorbent by the MEPS method using a laboratory-made programmable apparatus. Three functionalized SBA-15 nanoporous sorbents were evaluated for this purpose and 3-[bis(2-hydroxyethyl)amino]propyl-triethoxysilane (HPTES)-SBA-15 showed superior efficiency. Only 2 mg of the adsorbent packed in a syringe’s hub was sufficient for a quantitative recovery of EA. Different factors influencing the performance of the MEPS method such as the nature of desorption solvent, elution volume, pH, number of extraction cycles (draw–eject) and sample volume were carefully studied and optimized. Under the optimized conditions, a detection limit of 0.8 mg/L and a linear calibration curve with an R2 of 0.9995 were obtained for EA. The average recovery of the analyte for juice samples was 97.6% with a relative standard deviation of 4.4% for six replicates. The method was successfully applied to the HPLC determination of EA in pomegranate and grape juice samples.

Determination of ellagic acid, flavonoids and goshonoside-F5 in Rubi Fructus by HPLC

High-performance liquid chromatographic coupled with variable wavelength detection (HPLC-VWD) has been developed for simultaneous determination of 5 analytes including ellagic acid, quercetin, kaempferol-3-O-beta-D-rutinoside, tiliroside and kaempferol, and high-performance liquid chromatographic with an evaporative light scattering detector (HPLC-ELSD) has been established to determine goshonoside-F5 in extract of Rubi Fructus. Chromatographic separations were carried out on an Agilent ZORBAX SB-C18 column (4.6 mm x 250 mm, 5.0 microm). All calibration curves of reference standards revealed good linearity (R2 > 0.999 5) within the concentration ranges tested. The method limits of detection ranged 0.297-90.144 ng and the method limits ofquantitation ranged 0.990-300.480 ng, respectively. Recoveries of 6 analytes were from 97.11% to 101.7%, with RSD less than 2.1%. The result shows that amounts of the 6 analytes in the samples from 16 localities were found to be different. The higher latitude of growing environment, the more ellagic acid in herb. The content of total flavonoids in sample from east localities were higher than that in middle and west localities, and the content of goshonoside-F5 in Bozhou, Anhui province was higher than others. This method was found to be simple, accurate, sensitive with good repeatability. Those results might serve as a sound foundation for further study, quality control and application of Rubi Fructus.

Determination of ellagic acid by fluorescence quenching method with glutathione capped CdTe quantum dots as the probe

A sensitive and simple method for the determination of ellagic acid (EA) was developed based on the fluorescence quenching effect of EA for glutathione (GSH)-capped CdTe quantum dots (QDs). Under optimum conditions, a good linear relationship was obtained from 0.3118 to 55.0 μg mL−1 with a correlation coefficient of 0.9983, and the detection limit (3σ/K) was 0.0936 μg mL−1. The fluorescence quenching mechanism has been proposed on the basis of electron transfer supported by ultraviolet-visible (UV-vis) absorption, fluorescence (FL) spectroscopy and the measurement of fluorescence lifetime. The method has been applied to the determination of EA in synthetic samples and fresh urine samples of healthy humans with satisfactory results. The proposed method manifested several advantages such as high sensitivity, short analysis time, low cost and ease of operation.

Determination of Ellagic Acid in Strawberries, Raspberries and Blackberries by Square-Wave Voltammetry

Determination of Ellagic Acid in Strawberries, Raspberries and Blackberries by Square-Wave Voltammetry

The quantification of ellagic acid in the crude extract of Phyllanthus amarus Schum. & Thonn. (Euphorbiaceae)

Phyllanthus amarus Schum. & Thonn. (Euphorbiaceae), already well known for its antiviral, antihyperglycaemic and antihepatotoxic effects, is also investigated for its antimalarial activity. The major constituent of the crude extract of the whole plant was isolated and identified in this research to be ellagic acid, for which antiplasmodial activity already has been reported. Because of the potential of the plant and the interesting properties of ellagic acid, an analytical method can be useful for the standardisation of the extracts to allow further biological and pharmacological investigations. In order to obtain an easily performable and inexpensive method, an HPLC analysis was developed and validated. The samples were dissolved in DMSO, ultrasonicated for 15 min, and diluted with 50% methanol. Analysis was performed using water and methanol containing 0.06% TFA and the peaks were detected at 254 nm. Ellagic acid showed a linear relationship in the range of 1.74-20.91 µg/mL and a single-point calibration was allowed. The method was shown to be precise with respect to time (RSD of 1.84%, 3 days, n = 6) and concentration (RSD of 2.54%, 3 levels, n = 6). The overall mean content of ellagic acid was 2.06%. A recovery experiment was performed and it showed an accuracy of 100.4%. Based on the obtained results, it can be concluded that the newly developed method is suitable for its purpose, namely the determination of ellagic acid in the crude extract of P. amarus.

Polarographic (DCP & DPP) Determination of Ellagic Acid in Strawberries & Pharmaceutical Formulations

AbstractDirect Current (DC) and Differential Pulse (DP) Polarographic methods have been developed for the qualitative as well as quantitative analysis of ellagic acid in plant products (i.e. strawberries) and pharmaceutical formulations (Ellagic insurance formula). Ellagic acid produces a well‐defined polarographic wave/peak in pyridine hydrochloride at pH 6.8 ± 0.1 with E1/2 /Ep = −1.298 V vs. SCE.The wave/peak height is found to be proportional to the concentration of ellagic acid. The developed procedure was used for the analysis of ellagic acid in an extract of strawberries (Fragaria × ananassa) and pharmaceutical formulations. Statistical treatment of the observed polarographic data revealed high accuracy and good precision of determination.The work has been supplemented by FTIR screening of the sample. The percentage of ellagic acid in pharmaceutical formulations was determined by the developed method, which is in close agreement with the labeled amount.