Determination Of Adriamycin Research Articles

Determination of adriamycin in cell culture media by using excitation-emission matrix fluorescent spectra coupled with second-order calibration

This paper reports a simple and rapid method for quantitative analysis of adriamycin in cell culture media without any pretreatment procedure. It combined second-order calibration methods based on the alternating trilinear decomposition (ATLD) and the alternating normalization-weighter error (ANWE) algorithms, respectively, with excitation-emission matrix fluorescent spectra. When the component number in the case was set to 2, the average recoveries of adriamycin in cell culture media were (100.5±1.8)% and (100.3±1.9)%, respectively. The satisfactory results showed that the second-order calibration method can be applied to directly quantify adriamycin in cell culture media. Moreover, in the analysis of cell culture media including four interferences which are the fluorescent materials in cell, the average recoveries attained from ATLD and ANWE with the factor number of 4 were (99.1±2.9)% and (99.2±3.1)%, respectively. It was found that second-order calibration method combined with excitation-emission matrix fluorescent spectra is appropriate for quantitative analysis of drug contents in cell culture media even in the presence of natural fluorescent interferences of cell.

Fluorescence Spectroscopic Investigation of the Interaction between β-Cyclodextrin and Adriamycin

The fluorescence spectroscopic technique has been used to demonstrate the formation of the inclusion complex of adriamycin(ADM) and β-cyclodextrin(β-CD).The effects of the reaction time,concentration and temperature on the inclusion reaction are investigated.The chemical stoichiometric ratio for the formation of the inclusion complexes is determined.The inclusion constant and thermodynamic parameters of the inclusion reactions at different temperatures are calculated.At 33 ℃ and pH=7.0,the inclusion constant,K is 2.98×106 L/mol.The K value diminishes with the increase of temperature.Thus,the inclusion reaction is an enthalpy driving and spontaneous exothermic reaction.After ADM and β-CD form the inclusion complex,the fluorescence intensity is increased because β-CD can increase the natural fluorescence of ADM.Therefore,the fluorescence spectroscopic technique is an excellent technique for the investigation of the inclusion reaction of ADM and β-CD.The linear regression equation of the quantitative determination of ADM by β-CD sensitized fluorescence method is F=5.64×108c+47.26,with a correlation coefficient of 0.9985.The detection limit is 6.30×10-8 mol/L.

Utility of Ion-Pair Chromatography for Analysis of Some Anthracyclines in Plasma and Urine

A high performance liquid chromatographic assay was developed and validated for a simultaneous determination of adriamycin and its metabolites adriamycinol and adriamycinone as well as daunomycin in plasma. Acetonitrile and trichloroacetic acid were employed in removing proteins and extracting the drugs and metabolites into the supernatant for HPLC. Enhancement of chemical stability of the anthracyclines during sampling procedure was investigated using 0.01 M γ-cyclodextrin. The processed samples were chromatographed using an ODS/TM column (150 × 4.6 mm I.D.) eluted with a mobile phase composed of 35% acetonitrile and 65% (0.1M) monobasic phosphate, containing 0.3% heptafluorobutyric acid, pH 3. The detection was performed fluorimetrically at emission and excitation wavelengths of 555 and 460 nm, respectively. The described method could be directly applied for analysis of tested anthracyclines in human urine without sample pretreatment.

Determination of adriamycin in plasma and tissue biopsies

A simple, rapid and sensitive method for the extraction and high-performance liquid chromatographic analysis of adriamycin in tissue and plasma is described. Tissue (5-100 mg) and plasma (1 ml) samples underwent a C18 Sep-Pak extraction into methanol. Chromatography was performed on a muBondapakphenyl column using a mobile phase of acetonitrile-0.1 M ammonium formate (pH 4.0) with a flow-rate of 2 ml/min. Fluorometric detection was used with an excitation of 480 nm and an emission of 550 nm. The procedure produced a linear curve for the concentration range 25-1000 ng/ml. The development of the assay produced rapid, repeatable and accurate results for both small tissue samples and plasma.

A highly sensitive method for the determination of adriamycin and adriamycinol in human plasma using HPLC with fluorimetric detection

A simple and very sensitive HPLC method, for the simultaneous determination in human plasma of adriamycin and its metabolite adriamycinol, is described. Plasmas from patients were stored frozen. Thawed samples were extracted by absorption of anthracyclin onto a small C18 column. After evaporation of the eluate and reconstitution of the residue with methanol (100μL), 30 to 40μL of the mixture were injected into the chromatograph. Separation was obtained using an RP 8 column with a mobile phase of formate buffermethanol-acetonitrile (50∶23∶27, v/v). A spectrofluorimeter was used as detector. The limit of sensitivity of the assay was 50 pcg/ml of plasma.

Determination of adriamycin and its fluorescent metabolites in human plasma by HPLC.

We describe a method for measuring adriamycin and its metabolites, adriamycinol and adriamycinone in plasma, using reversed phase HPLC and fluorescence detection. The lower limit of detection is approximately 1 ng/mL for each compound. An extraction technique for serum is described which is capable of an almost equal recovery (greater than 93%) of adriamycin and metabolites without interference from endogenous components of plasma and from other common drugs. Within-day and day to day coefficients of variation are estimated.

Determination of adriamycin, adriamycinol and their 7-deoxy aglycones in human serum by high-performance liquid chromatography

A reversed-phase isocratic high-performance liquid chromatographic assay is described for the measurement of adriamycin, adriamycinol and their 7-deoxyaglycones in human serum. The lower limit of detection in serum is 3 ng/ml for adriamycin and 1 ng/ml for adriamycinol and the 7-deoxyaglycones with coefficients of variation for k′ of less than 5% throughout the day. An extraction technique for serum is described which is capable of an almost equal recovery (>77%) of adriamycin, metabolites and daunorubicin (the internal standard) without interference from endogenous components of serum. Serum concentrations of metabolites 15 min after intravenous bolus administration of 40 mg/m 2 adriamycin in two different patients were 26.5 and 16.6 ng/ml for adriamycinol; 109.8 and 5.8 ng/ml for the adriamycinol 7-deoxyaglycone and 21.4 and 17.1 ng/ml for the adriamycin 7-deoxy- aglycone. A total of six metabolites of adriamycin were detected in the two patients using this methodology.

Determination of adriamycin (doxorubicin) and related fluorescent compounds in rat lymph and gall by high-performance liquid chromatography

The concentrations of adriamycin (ADM) and related fluorescent compounds in lymph and gall were determined by high-performance liquid chromatography (HPLC) after a single intravenous injection into AH 109A tumour-bearing rats. HPLC was carried out by using Zorbax Sil as the stationary phase and chloroform-isopropanol-acetic acid-water-sodium acetate buffer (pH 4.5) (100:100:14:14:1) as the mobile phase, with a fluorescence spectrophotometric detector at an excitation wavelength of 470 nm and an emission wavelength of 585 nm. The detection limit for ADM was down to 1.0 ng/ml. In the thoracic duct lymph, the concentration of total ADM equivalent values (total ADM values) was maximal 30 min after injection and, after a subsequent decrease, increased gradually from 60 to 180 min. The ratio of total ADM in lymph to that in plasma at 180 min was 1.5 times that at 30 min. In gall, the total ADM showed a maximal level of 20.0 μg/ml at 30 min.

Determination of adriamycin and daunorubicin in urine by high-performance liquid chromatography with laser fluorometric detection

A separation and detection scheme is presented for the determination of the antitumor drugs adriamycin and daunorubicin in human urine. Separation is accompished by reversed-phase high-performance liquid chromatography and the drugs are detected down to the low picograms level by laser excited fluoresence using a unique fiber optic based flow-cell. Excellent detector selectivity and linearity are reported, and some of the factors influencing the performance of the detector are discussed. Possible extension of the procedure to other biologically important determinations are mentioned.

Determination of adriamycin in liposomes by hihg-performance liquid chromatography using a fluorescence detector

Determination of adriamycin in liposomes by hihg-performance liquid chromatography using a fluorescence detector

Liquid Chromatographic Analysis of Adriamycin and Metabolites in Biological Fluids

Abstract A definitive approach to the analysis of plasma, bile, and urine levels of the widely used antitumor drug Adriamycin using two complementary high performance liquid chromatographic systems, one normal phase and the other reversed phase, is described. Sensitivity in the 2–10 picomole per sample range is achieved by means of fluorescence detection. The use of the two systems in parallel provides an unequivocal basis for the characterization and determination of Adriamycin and its principal metabolite, adriamycinol, and enables the measurement of anthracycline fluorescent aglycones and conjugates, as well.