Detection Of Red Blood Cells Research Articles

Lower initial red blood cell count in cerebrospinal fluid predicts good functional outcome in patients with spontaneous subarachnoid haemorrhage.

Red blood cell (RBC) degradation after subarachnoid haemorrhage (SAH) negatively affects functional outcome. Although the detection of RBCs in the cerebrospinal fluid (CSF) is a widely available part of neurological routine diagnostics, the prognostic value as a biomarker remains unclear. This study was undertaken to investigate whether CSF RBC count correlates with established radiological markers of SAH volume and whether the CSF RBC count can predict functional outcome in SAH patients. A total of 121 consecutive spontaneous SAH patients were retrospectively analyzed. CSF was collected from external ventricular drain as part of routine diagnostic procedures. We used multivariable binary logistic regression to investigate associations between CSF RBC counts and functional outcome 3 months after SAH or hospital survival. Good functional outcome was defined as modified Rankin Scale ≤ 2. Patients' age was 60 ± 14 years, and the median admission Hunt & Hess grade (H&H) was 4. CSF samples were collected 2 days after intensive care unit admission. High CSF RBC counts positively correlated with radiological measurements for SAH volume, for example, modified Fisher score (p = 0.002) and Hijdra ventricle score (p = 0.016). Multivariable regression analysis adjusted for age, H&H grade, modified Fisher and Hijdra scores showed that low CSF RBC counts predicted hospital survival (per 100,000 CSF RBCs: adjusted odds ratio [adjOR] = 0.74, 95% confidence interval [CI] = 0.61-0.89, p = 0.001) and good functional outcome after 3 months (per 100,000 CSF RBC: adjOR = 0.76, 95% CI = 0.60-0.96, p = 0.020). CSF RBC counts correlate with radiographic scores quantifying SAH volume and may serve as an early independent biomarker for hospital survival and good functional 3-month outcome in patients requiring ventriculostomy after SAH.

Human urogenital schistosomiasis in West and Sub-Saharan Africa migrants in Sardinia, Italy: A retrospective monocentric study.

Schistosoma (S.) haematobium is the aetiological agent of urogenital schistosomiasis endemic in Sub-Saharan Africa and the Middle East. Microhaematuria is strongly associated with schistosomiasis diagnosis. Praziquantel (PZQ) is the treatment of choice. We conducted a monocentric survey among African migrants from January 2017 to December 2018. The diagnosis of S. haematobium was performed by direct microscopic examination of urine. The treatment was PZQ 40 mg/Kg/die for three days. We enrolled 91 male patients with a median age of 20.2 years (IQR 18.9-23.4)]. Forty-five (49.5%) described a history of haematuria. Sixteen (17.6%) evidenced the presence of red blood cells (RBCs) during urine microscopy. Eighteen (19.8%) had urogenital schistosomiasis. Their median white blood count (WBC) was 5.15 x 109/L (IQR 4.45-6.08) and it was 6.37 x 109 /L (IQR 5.14-8.27), p = 0.009, after 15 days from treatment. Baseline eosinophil count was 0.5 x 109/L (IQR 0.3-0.6) and 0.7 x 109/L (IQR 0.2-1.9; p = 0.032). According to the univariate analysis, origin from Mali [odds ratio (OR) 3.6 (CI 1.2-10.9), p = 0.022] and microscopic evidence of RBCs [OR of 10.7 (CI 2.5-45.1), p = 0.001] were main predictors of urogenital schistosomiasis diagnosis. One (5.6%) treatment failure was registered. Three (16.7%) patients had bladder cancer. Detection of RBCs was a significant predictor of S. haematobium infection and could be used as a screening method in migrants coming from endemic areas. Early urogenital schistosomiasis diagnosis and ultrasound diagnostic tools are crucial for reducing the risk of potential neoplastic evolution.

Comparative study on urine dry chemistry method, urinary sediment analyzer and microscope in the urine examination of urologic neoplasms patients

Objective To investigate the value of urine dry chemistry method, urinary sediment analyzer and microscope in the diagnosis of urologic neoplasms. Methods The clinical data of 80 inpatients with urologic neoplasms treated in Nantong tumor hospital from June 2015 to October 2017 were retrospectively analyzed, and all selected cases were confirmed by pathological examination. The urine specimens of all patients were examined by urine dry chemistry method, urinary sediment analyzer and microscope. The detection of white blood cell (WBC) and red blood cell (RBC) in urine of patients were analyzed. Results The positive detection rate of WBC by urine dry chemistry method, urinary sediment analyzer was higher than that by and microscope (P<0.05). The positive detection rate of RBC by urine dry chemistry method, urinary sediment analyzer was higher than that by and microscope (P<0.05). The microscope examination result was taken as the reference standard, the sensitivity and specificity of urine dry chemistry method in the detection of WBC were slightly lower than those of urinary sediment analyzer. The sensitivity and specificity of urine dry chemistry method in the detection of RBC were slightly higher than those of urinary sediment analyzer. The total cost of automatic urine dry chemistry analyzer was less than that of other two inspection instruments, and the utilization rate of instrument was higher. Conclusions The three urine examination methods in the detection of RBC and WBC have some limitations and advantages. When selecting the clinical detection method, the appropriate method should be selected according to the purpose of the test. If necessary, it can be used in combination to reduce the rate of missed detection and improve the accuracy of detection. Key words: Urologic Neoplasms; Urine; Urinalysis; Microscopy