Detection Of Methyl Parathion Research Articles

Smartphone-based plant-wearable microfluidic sensor with self driven electrolyte for in-situ detection of methyl parathion

Smartphone-based plant-wearable microfluidic sensor with self driven electrolyte for in-situ detection of methyl parathion

Enzyme-linked immunoassay for simultaneous detection of methyl parathion and sibutramine in apple cider vinegar.

Methyl parathion, a highly toxic, efficient, and persistent organophosphorus pesticide, is widely used in China. Sibutramine, a non-amphetamine central nervous system depressant, helps lose weight by disrupting hormone regulation, stimulating sympathetic nerves, and suppressing appetite. However, some unethical businesses fail to properly handle raw materials in foods like apple cider vinegar, leading to residual methyl parathion in apples or illegal excessive addition of sibutramine. Therefore, it is imperative to develop an immunoassay for the rapid detection of methyl parathion and sibutramine. The corresponding two haptens were prepared and coupled with the carrier proteins according to methyl parathion-sulfur-bovine serum protein (BSA)/chicken ovalbumin (OVA)-sibutramine (20 : 1 : excess, 15 : 1 : excess, 10 : 1 : excess, and 5 : 1 : excess), and sibutramine-BSA/OVA-methyl parathion (20 : 1 : excess, 10 : 1 : excess: 5 : 1 : excess, and 0 : 1 : excess). The result shows that the inhibition rate of the antibody obtained by methyl parathion-BSA/OVA-sibutramine (20 : 1 : excess) was higher than that of sibutramine-BSA/OVA-methyl parathion, which was 67.93%, and the concentration of methyl parathion was 8.65 ng mL-1 at this inhibition rate. Thus, methyl parathion-BSA/OVA-sibutramine (8.65 : 1 : excess) and the corresponding antibodies were selected for subsequent method establishment. By changing the concentration of the coating and antibody, the inhibition rate was found when the coating was 0.125 ng mL-1 and the antibody was diluted 4000 times. The antibody was used to develop a standard curve for the detection of sibutramine at the half-maximum inhibitory concentration (IC50) is 4.59 ng mL-1, the limit of detection (IC10) is 2.21 ng mL-1, the detection range is 2.89 to 7.28 ng mL-1, methyl p-phosphorus at the half-maximum inhibitory concentration (IC50) is 15.34 ng mL-1, the limit of detection (IC10) is 0.42 ng mL-1, the detection range is ng mL-1. Under these conditions, the recovery rate was between 88% and 102%, within reasonable limits, indicating the successful establishment of a rapid enzyme-linked ELISA assay.

Facile synthesis of novel porphyrin-based covalent organic frameworks integrated with Au nanoparticles for highly sensitive detection of organophosphorus pesticide residues

Organophosphorus pesticides (OPs) residues in agricultural products such as vegetables, fruits, and grains pose a threat to food safety and human health. In this work, a novel and highly sensitive electrochemical biosensor was developed for OPs residues detection in real sample, based on the immobilization of acetylcholinesterase (AChE) by electrostatic adsorption on a glassy carbon electrode (GCE) modified with composite nanomaterials of porphyrin-based covalent organic frameworks (p-COFs) loaded by gold nanoparticles (AuNPs) and poly (diallyldimethylammonium chloride) (PDDA). Due to the synergistic effect of p-COFs, AuNPs and PDDA, the fabricated biosensor displayed the remarkable properties with the high electrocataytic activity, and excellent biocompatibility, resulting in a significant signal amplification of as-prepared biosensor. Under optimized conditions, the biosensor showed excellent sensing performances for the detection of methyl parathion (MP), with wide linear ranges from 1.9 × 10−9–3.8 × 10−5 M and a low detection limit as low as 2.3 × 10−10 M, as well as the satisfactory selectivity, excellent reproducibility, anti-interference properties and good stability. It was also successfully applied to the monitoring of MP content in vegetables and fruits, which may be explore a new opportunity for the potential utilization in analysis field.

Highly sensitive methyl parathion electrochemical sensor designed from expired mung bean-derived porous carbon embellished with cerium oxide nanoparticles

Methyl parathion (MP) is one kind of highly toxic, efficient, and broad-spectrum organic phosphorus insecticides, which may prevent and control various pests on crops. However, the unscientific usage of MP has caused serious harm to humans and ecological environment. It is very necessary to design a sensitive and selective MP analysis method to ensure the food safety and life health. In this work, we reported a low-cost and environmental-friendly strategy to prepare the expired mung bean derived porous carbon (EMBPC) with interconnected carbon conductive network and large specific surface area, which was further decorated by cerium oxide (CeO2) nanoparticles. EMBPC possessed good conductivity property and electrochemical active area, which effectively boosted the charge transfer efficiency. CeO2 nanoparticles with good biocompatibility and high catalytic capacity promoted the efficient adsorption of MP on the surface of sensing electrode due to the affinity of CeO2 nanoparticles towards the phosphate groups of MP. Furthermore, EMBPC with porous carbon conductive structure could effectively make up for the inherent disadvantage of CeO2 nanoparticles in the term of electrical conductivity. The fabricated EMBPC@CeO2/GCE sensor showed highly sensitive MP detection property with low limit of detection (LOD) of 9.028 nM. The satisfactory repeatability, reproducibility, and anti-interference ability could be achieved at the fabricated MP electrochemical sensor. For the detection of MP in food samples, the EMBPC@CeO2/GCE sensor showed satisfactory recovery rates of 95.25–112.31 % and low RSD of 1.76–4.05 %, which suggested the good application prospect of the EMBPC@CeO2/GCE sensor.

Bifunctional ZrO 2@ZIF-90 nanozyme with high phosphohydrolase activity for sensitive electrochemical detection of methyl parathion

Bifunctional ZrO ₂@ZIF-90 nanozyme with high phosphohydrolase activity for sensitive electrochemical detection of methyl parathion

Synergistic Manganese Cobalt Phosphide core-shell for the Electrochemical Detection of Methyl Parathion in Food Sample

The development of a robust electrocatalyst for the electrochemical sensor for hazardous pesticides will reduce its effects on the ecosystem. Herein, we synthesized the robust manganese cobalt phosphide (MnCoP) - Core-shell as an electrochemical sensor for the determination of hazardous pesticide methyl parathion (MP). The MnCoP- Core-shell was prepared with the sustainable self-template route can help with the larger surface area. The Core-shell structure of MnCoP possesses a higher active surface area which increases the electrocatalytic performance and is utilized to improve the electrochemical MP reduction with the synergism of the core and shell structure. Remarkably, it realizes the higher sensitivity (0.014 μA μM−1 cm−2) of MnCoP- Core-shell/GCE achieves towards MP with lower limit of detection (LoD 50 nM) and exceptional recovery rate of MP in vegetable samples are achieved with the differential pulse voltammetry (DPV) technique. The MnCoP- Core-shell electrode reserved their superior electrochemical performances with high reproducibility and repeatability. This prominent activity of the MnCoP core-shell towards the MP in real sample analysis, makes it a promising electrochemical sensor for the detection of MP.

Facile fabrication of single-walled carbon nanotubes@cerium oxide composite sensing platform for ultrasensitive electrochemical analysis of methyl parathion

We proposed a simple and feasible strategy to fabricate the nano-structured cerium oxide (CeO2) nanoparticles decorated single-walled carbon nanotubes networks (SWCNT@CeO2), which was utilized to fabricate the SWCNT@CeO2/GCE sensor for detecting methyl parathion (MP) with high sensitivity. SWCNT possessed the unique one-dimensional hollow nanostructure with excellent conductivity performance, which boosted the charge diffusion rate. CeO2 with good biocompatibility and high catalytic capacity could achieve the adsorption of MP molecules on the surface of sensing electrode due to the affinity of CeO2 nanoparticles towards the phosphate groups of MP. Furthermore, SWCNT could effectively make up for the inherent disadvantage of CeO2 nanoparticles in the term of electrical conductivity. The fabricated SWCNT@CeO2/GCE sensor achieved the ultrasensitive MP electrochemical analysis property (LOD: 9.12 nM). The satisfactory repeatability, reproducibility, anti-interference abilities were obtained at the fabricated sensor. Regarding the electrochemical detection of MP in real food samples, both satisfactory recovery rates of 87.68–107.31% and low RSD of 1.09–2.65% suggested the favorable application prospect of SWCNT@CeO2/GCE sensor in the field of pesticide residue analysis.

An in situ one-step route for the synthesis of Fe3O4@ZIF-8 core-shells with a high-peroxidase-like performance for the colorimetric detection of methyl parathion

Methyl parathion (MP) is a broad-spectrum agricultural insecticide. Human exposure to MP can affect the nervous, cardiovascular, and hepatic systems. In this research, a core–shell Fe3O4@zeolitic imidazolate framework-8 (ZIF-8) is developed as a peroxidase mimic for the rapid and simple colorimetric detection of MP. Fe3O4 nanoparticles were wrapped with ZIF-8 by a hydrothermal method to obtain core–shell structural Fe3O4@ZIF-8 nanocomposites. The synthesized Fe3O4@ZIF-8 possessed mimetic properties and effectively catalyzed the conversion of transparent solution to blue upon the oxidation of 3, 3′, 5, 5′-tetramethylbenzidine (TMB) by H2O2. Further, based on a classical reaction through acetylthiocholine chloride (ATCl) and cholesterol oxidase (ChOx), acetylcholinesterase (AChE) can generate H2O2, and that MP can exert an inhibitory effect on AChE. Within the range of 10 to 200 µg/L, the MP concentration demonstrated a remarkable linear response with the absorbance under optimum conditions, with a detection limit of 6.3 μg/L. Finally, the proposed approach enabled the detection of MP in cabbage. As an artificial peroxidase, Fe3O4@ZIF-8 demonstrated greater affinity for the substrate than horseradish peroxidase, which has a great application prospects in food safety and environmental safety.

A Rapid and Easy Procedure of Enzyme Biosensor based on Nitrogen-Doped Graphene for Detection of Methyl Parathion in CHM

Methyl parathion (MP), an organophosphorus pesticide that is frequently used to control pests during the planting process of Chinese herbal medicine (CHM), has been used without sufficient control, leading to excessive residues on the surface of CHM, which has a serious impact on the quality and safety of CHM and their preparations. Consequently, it is crucial to carefully control MP during the cultivation, processing, and manufacture of CHM. Based on this, nitrogen-doped graphene (N-Gr) with a high conductivity and chitosan (CS) with good stability were used to modify the glassy carbon electrode (GCE). Subsequently, the prussian blue (PB) with catalytic activity and gold nanoparticles (AuNPs) with excellent biocompatibility were deposited by the electrodeposition method to form the AuNPs/PB/CS@N-Gr/GCE. Then, the acetylcholinesterase (AChE) was effectively immobilized on the electrode surface by covalent bonding between AuNPs and AChE through gold-sulfhydryl bonds. Finally, a rapid and easy procedure of enzyme biosensor (AChE/AuNPs/PB/CS@N-Gr/GCE) for sensitive detection of MP pesticide residues was fabricated. Herein, PB catalyzes the redox reaction of thiocholine, which is produced when acetylthiocholine iodide (ATCHI) undergoes efficient hydrolysis catalyzed by AChE. This process effectively promoted electron transfer, amplifying the sensor’s response signal. After the experimental conditions are optimized, the limit of detection (LOD) for MP is found to be 9.47 × 10−5 μg ml−1. Exhibits a good linear relationship within the concentration range of 1 × 10−3 μg ml−1 ∼ 1 × 101 μg ml−1. Significantly, the fabricated enzyme biosensor excels in swiftly and sensitively detecting trace amounts of MP in real examples. Furthermore, it exhibits robust stability and reproducibility. The excellent performance of this enzyme biosensor not only offers a rapid and easy way to identify and find minute amounts of trace MP pesticide residues in CHM, but also serves as a technical guide for the creation of new, portable, and on-site pesticide residue detection technology for law enforcement.

Ultrasensitive electrochemical sensing platform based on Zr-based MOF embellished single-wall carbon nanotube networks for trace analysis of methyl parathion

In this work, a novel electrochemical sensor was designed based on Zr-based metal–organic framework (UIO-66) nanoparticles embellished single-wall carbon nanotube networks (SCN) for the detection of methyl parathion (MP). The synergistic combination of SCN and UIO-66 nanoparticles could fully exert each advantage to boost the MP electrochemical sensing analysis performance. SCN with one-dimensional hollow nanostructures formed three-dimensional interconnected carbon conductive networks, which possessed excellent conductive properties and acceptable specific surface area, enabling efficient transport of electrons and ions. UIO-66 nanoparticles with Zr-OH groups contributes and porous structure to the good affinity for phosphate groups on MP molecules, which enhanced the selective recognition and enrichment capability of sensing electrode towards MP. Under the multifunctional integration of SCN and UIO-66 nanoparticles, the SCN@UIO-66/GCE sensor could present satisfactory MP electrochemical detection performance (LOD: 8.05 nM, linear MP concentration range: 0.01–10 μM). The satisfactory practical property was realized at the SCN@UIO-66/GCE sensor with satisfactory recovery rates of 98.28–101.95 % and low RSD of 1.23–3.01 % for the electrochemical detection of MP in orange juice and lettuce samples.

A ratiometric fluorescence probe based on dual quantum dots for methyl parathion detection in agricultural products

Methyl parathion (MP) is an organic phosphorus pesticide widely used for pest control of various crops, however, the residual MP can enter the human body and damage the nervous system. Therefore, it is necessary to effectively monitor MP residues. Based on dual-fluorescent quantum dots, this study establishes a ratio fluorescence sensing platform for MP detection, wherein the probe is β-cyclodextrin (β-CD) modified molybdenum disulfide quantum dots (MoS2 QDs) called β-CD-MoS2 QDs. Under alkaline conditions, MP is hydrolyzed into p-nitrophenol (p-NP) and enters the hydrophobic cavity of β-CD, quenching the blue fluorescence of MoS2 QDs whose quenching degree of fluorescence intensity (I400) correlates with the concentration of MP, while the fluorescence intensity (I540) of gQDs remains unchanged and is selected as reference signal. The quantitative detection of MP is conducted by calculating the ratio of fluorescence intensities (I540/I400). The probe detected MP in the range of 0.05–25 ppm with a detection limit of 0.032 ppm which is proved to meet the requirements for biological sample detection according to the methodological validation results. The probe has been successfully applied for the detection of MP in apples and vegetables, confirming its applicability for MP detection in crops.

Metal-organic framework-based multienzyme cascade bioreactor for sensitive detection of methyl parathion

In this study, a cascade nanobioreactor was developed for the highly sensitive detection of methyl parathion (MP) in food samples. The simultaneous encapsulation of acetylcholinesterase (AChE) and choline oxidase (CHO) in a zeolitic imidazole ester backbone (ZIF-8) effectively improved the stability and cascade catalytic efficiency of the enzymes. In addition, glutathione-stabilized gold nanoclusters (GSH-AuNCs) were encapsulated in ZIF-8 by ligand self-assembly, conferring excellent fluorescence properties. Acetylcholine (ATCh) is catalyzed by a cascade of AChE/CHO@ZIF-8 as well as Fe(II) to generate hydroxyl radicals (·OH) with strong oxidizing properties. The ·OH radicals then oxidize Au(0) in GSH-AuNCs@ZIF-8 to Au(I), resulting in fluorescence quenching. MP, as an inhibitor of AChE, hinders the cascade reaction and thus restores the fluorescence emission, enabling its quantitative detection. The limit of detection of the constructed nanobioreactor for MP was 0.23 µg/L. This MOF-based cascade nanobioreactor has great potential for the detection of trace hazards.

Subtle adjustment of the cyclic potential on electro-activated glassy carbon electrodes for sensitive sensing of methyl parathion.

Facile electro-activated glassy carbon electrodes (e-GCEs), which are prepared in electrolyte solution with a certain potential for a few seconds, have been verified to improve analytical performance toward not a few electro-active molecules recently. Nevertheless, how and why the potential plays an important role is not clear, and has even not received enough consideration. In this paper, we found that the mode and the range of applied potential significantly impacted the sensitivity of methyl parathion (MP), which is a typical pesticide with the electro-active group of -NO2. Compared with constant potential, the e-GCE with cyclic potential provided a much more stable baseline during MP detection. Additionally, the electro-oxidation peak current of MP at around -0.1 V on it was higher than another changeable potential (constant current). What's more interesting, with cyclic potential for 50 segments from -2 to 1.5 V, the peak current value increased by 30 times in comparison with a bare GCE, but only 2 times from -2 to 1 V. Then after systematic investigation including structures of the electrode surface and functional groups, we speculated that the produced group of O-CO in the process of activation and remaining groups of C-O and CO on the bare GCE surface are beneficial for adsorbing MP molecules leading to enhanced peak current. Employing the proposed e-GCE, the limit of detection of MP reached 0.015 μM and the reproducibility was perfect. This work elucidates the potent impact of electro-activation potential parameters on electroanalysis behaviors.

Development of a bioluminescence resonance energy transfer Quenchbody sensor for the detection of organophosphorus pesticides in water bodies

Rapid and precise quantification of organophosphorus pesticides (OPPs) in environmental water bodies is crucial for evaluating ecological risks and safeguarding human health. Traditional instrumental methods are complex, time-consuming, and expensive, while enzyme-based biosensors suffer from instability and require a constant supply of substrates. Hence, there is an urgent need for a fast, simple, and sensitive biosensor for OPPs. In this study, we developed a novel non-enzymatic biosensor for the detection of methyl parathion (MP) by employing the bioluminescence resonance energy transfer (BRET) Q-body strategy. Optimizing the spacer arm and screening fluorescent dyes identified the R6G BRET MP Q-body sensor with the best performance. Key parameters affecting the sensor's detection performance were optimized by using single-factor experiments. Under optimal conditions, the detection exhibited a detection limit of 5.09 ng·mL−1 and a linear range of 16.21–848.81 ng·mL−1. The sensor's accuracy was validated using standard recovery experiments, yielding a recovery rate of 84.47 %–102.08 % with a standard deviation of 1.93 %–9.25 %. The detection results of actual water samples demonstrate that this fast, simple, and highly sensitive BRET Q-body sensor holds great promise for practical water quality monitoring.

Gold nanoclusters anchored manganese dioxide nanocomposites with high structural stability for sensitive detection of methyl parathion

A long-standing goal has been to create a high-energy transfer system for food safety detection. Herein, gold nanoclusters-anchored manganese dioxide (AuNCs@MnO2) nanocomposites are designed by using co-template assembly. Bovine serum albumin is utilized as both skeletons of nucleation and stabilizer to guide the synthesis of the AuNCs@MnO2 nanocomposites under physiological conditions without the use of any potent oxidants and the hazardous organic solvent. The AuNCs@MnO2 nanocomposites exhibit good energy transfer efficiency and structural stability even under high salt conditions and a wide range of pH values. A sensitive sensing platform for the detection of methyl paraoxon has been constructed as a result of the excellent performance of AuNCs@MnO2 nanocomposites. Acetylcholinesterase catalyzes the conversion of the substrate to produce thiocholine, which induces the decomposition of MnO2 and the fluorescence recovery of AuNCs. Coupling with the pesticide inhibition effect, the platform can be employed to quantify methyl parathion with a detection limit of 3.1 pg mL−1. The AuNCs@MnO2-based platform displays high stability and anti-interference capability in food and environment samples, endowing practical application in food safety monitoring.

Electrochemical detection of methyl parathion using zirconium dioxide@single-walled carbon nanotubes nanocomposite modified glassy carbon electrode

In this work, the multifunctional nanocomposite of zirconium dioxide (ZrO2) nanoparticles and single-walled carbon nanotubes (SCN) was prepared to modify the glassy carbon electrode (GCE) for the fabrication of ZrO2@SCN/GCE sensor. The fabricated nanocomposite electrochemical sensor was successfully used to achieve the efficient and sensitive detection of methyl parathion (MP). For the ZrO2@SCN nanocomposite, ZrO2 nanoparticles could promote the enrichment of MP molecules on the electrode surface of ZrO2@SCN/GCE sensor because of the interplay between Zr-O groups and phosphorus groups of MP molecules; SCN presented efficient one-dimensional charge transport channels, which achieved the excellent conductivity property of carbon material. Moreover, SCN could greatly compensate for the non-conductivity of ZrO2 nanoparticles. The functional combination of ZrO2 nanoparticles and SCN enhanced the electrochemical detection performance of MP. The ZrO2@SCN/GCE sensor exhibited good MP detection property with low limit of detection of 6.15 nM (pH = 7.0, MP concentration: 0.01–10 μM). The fabricated sensor also showed good practical application property for the detection of MP in real samples (Lake water and green tea).

A Colorimetric Sensor Enabled with Heterogeneous Nanozymes with Phosphatase-like Activity for the Residue Analysis of Methyl Parathion.

In this study, a colorimetric sensor was developed for the detection of organophosphorus pesticides (OPs) using a heterogeneous nanozyme with phosphatase-like activity. Herein, this heterogeneous nanozyme (Au-pCeO2) was obtained by the modification of gold nanoparticles on porous cerium oxide nanorods, resulting in synergistic hydrolysis performance for OPs. Taking methyl parathion (MP) as the target pesticide, the catalytic performance and mechanism of Au-pCeO2 were investigated. Based on the phosphatase-like Au-pCeO2, a dual-mode colorimetric sensor for MP was put forward by the analysis of the hydrolysis product via a UV-visible spectrophotometer and a smartphone. Under optimum conditions, this dual-mode strategy can be used for the on-site analysis of MP with concentrations of 5 to 200 μM. Additionally, it can be applied for MP detection in pear and lettuce samples with recoveries ranging from 85.27% to 115.87% and relative standard deviations (RSDs) not exceeding 6.20%, which can provide a simple and convenient method for OP detection in agricultural products.

Fabrication of methyl parathion electrochemical sensor based on β-cyclodextrin decorated single-wall carbon nanotubes

ABSTRACT Herein, an efficient and sensitive methyl parathion (MP) electrochemical sensor was fabricated by using β-cyclodextrin (β-CD) decorated single-wall carbon nanotubes (SCN), which were used to modify the glassy carbon electrode (GCE). The β-CD@SCN/GCE sensor achieved the sensitive MP detection. For the obtained β-CD@SCN nanocomposite, SCN could form three-dimensional reinforced carbon conductive network with good conductivity property and efficient charge transport channels; β-CD could promote the dispersion of carbon materials and boost the accumulation capability of MP on the surface of β-CD@SCN/GCE. The combination of β-CD and SCN significantly optimized the electrochemical property of β-CD@SCN/GCE sensor for the detection of MP. The β-CD@SCN/GCE sensor exhibited good MP electrochemical detection performance with low limit of detection of 7.23 nM. When used for the detection of MP in grape juice, the β-CD@SCN/GCE sensor exhibited good MP electrochemical sensing analysis performance with satisfactory recovery rates (96.54–98.18%) and relative standard deviation values (1.60–2.07%).

Facile synthesis of β-cyclodextrin decorated Super P Li carbon black for the electrochemical determination of methyl parathion

ABSTRACT Herein, a novel electrochemical sensor was designed for the sensitive determination of methyl parathion (MP) using the Super P Li carbon black@β-cyclodextrin (SPLCB@β-CD) decorated glassy carbon electrode (GCE). SPLCB with high electroactive area exhibited exceptional conductivity and good dispersive property, which is beneficial for forming the interlinked conductive network structure. β-CD could not only promote the uniform dispersion of SPLCB but also effectively realise the enrichment of MP molecules. Moreover, the existence of SPLCB could make up for the defect of poor conductivity of β-CD. Under the optimal experimental condition (pH = 7.0, enrichment time: 60 s). The collaborative cooperation of SPLCB and β-CD achieved the excellent MP detection property (LOD: 8.6 nM; concentration range: 0.01–10 μM). The SPLCB@β-CD/GCE sensor exhibited acceptable repeatability and reproducibility. In addition, the proposed sensor was successfully employed to detect MP in apple juice (Recoveries: 98.0–98.6%, RSD values: 3.68–4.63%).

Development of a whole-cell biosensor for detecting organophosphorus pesticide methyl parathion in the farmland soil

The evaluation of the bioavailability of pollutants in soil is crucial to accurately assess the pollution risk, and whole-cell biosensor is one of the important tools for such evaluation. This study aimed to develop a novel whole-cell biosensor for the detection of methyl parathion in soil using. First, a whole-cell biosensor was constructed by the screened methyl parathion hydrolase mpd gene, the existing specific induction element pobR, and the pUC19 plasmid skeleton. Then, the detection method of methyl parathion in soil extracts was established using 96-well microtiter plate as carrier and five whole-cell biosensors as indicator. The method was applied in the detection of methyl parathion in tested and field soil extracts. Taking E. coli DH5α/pMP-AmilCP with the best detection performance as an example, this biosensor had a detection limit of 6.21-6.66 µg/L and a linear range of 10-10 000 µg/L for methyl parathion in four soil extracts. E. coli DH5α/pMP-RFP and E. coli DH5α/pMP-AmilCP methods have good detection performance for the analysis of methyl parathion in soil extract samples. This biosensor method can help to quickly assess the bioavailability of methyl parathion in soil, and thus help to understand the risk of soil pollution caused by organophosphorus pesticide methyl parathion.