Detection Of anti-Toxoplasma Antibodies Research Articles

Serological Detection of Anti Toxoplasma Antibodies Among Pregnant Diabetic Women in Amedi – Kurdistan Region

This study investigated the presence of specific antibodies against Toxoplasma gondii among pregnant diabetic women in comparison with healthy pregnant non diabetic women in Amedia. Overall, 300 pregnant women were enrolled in this study. The studied women were divided in to 2 groups, the first group included 200 pregnant diabetic women. The second group (control group) included 100 healthy pregnant non diabetic women. Diabetes is an important factor that increases susceptibility and risk of various infections in the host. Seropositivity of anti-IgG T. gondii recorded 24.8% among pregnant female with HBA1C ≥ 7 and 15.2% among pregnant female with < 7 while rate of anti- IgM T. gondii showed as 2.5% between pregnant with ≥ 7, moreover rate of anti-IgG T. gondii among healthy pregnant women recorded as 50% with HBA1C ≥ 7 ,10.4% with HBA1C < 7 and 1% seropositive for anti-IgM T.gondii between control group with HBA1C ≥ 7. In present study rate of anti-IgG T. gondii higher in age group <30 compared with age ≥ 30 while rate of anti-IgM T. gondii were higher in age ≥30 compared with age <30 year. Higher rate of infection found in urban area compared with rural area in both groups. Own -Cat another parameter that showed that most infection occur between patients who have contact or own cat which the main source of infection with toxoplasmosis. Regarding exposure to soil 24% where seropositive for anti- IgG T. gondii and 19,2% where positive among patients no exposure to soil and the results not significant, while anti-IgM T. gondii found 2.7% patients were exposure to soil and 0.8% were seropositive between patients have no exposure to soil and the results statistically significant. Results related to good washing of the vegetables showed that 13% were seropositive for anti-IgG T. gondii and statistically the results were not significant. In the current study the seropositivity of anti-IgG T. gondii between patients who meat consumption half cooked were 11.1% and 12.1% among patients who meat consumption good cooked, also 1.2% seropositive for anti-IgM T. gondii among patients who meat consumption good cooked and results not significant statistically. The rate of seropositive of anti-IgG T. gondii among patients with miscarriage and without miscarriage reported as 7.1%, 12.8% respectively. The statistical difference was not significant, while the rate of seropositive of anti-IgM T. gondii among patients without miscarriage reported as 1.2% and statistically the difference was not significant. It is clear that the rate of seropositivity of anti-IgG T. gondii among illiterate patients were higher (15.4%), while among literate patients were 1.1% and this result was not significant. The rate of seropositive of anti-IgG T. gondii according to occupation which divided in to employee and unemployed showed that 19%, 10 % respectively, the rate of seropositivity of anti-IgM T. gondii among unemployed reported as 1.3% with no significance difference statistically. In conclusion, in the present study some lifestyle variable of patients had a p value < 0.05, including HBA1C ≥7, age, own cat and exposure of soil. This study provided serological evidence of an association between T. gondii infection and pregnant diabetes mellitus., which may help to guide future research, further studies should be conducted to elucidate the role of T. gondii in diabetes mellitus.

EFFECTS OF LATENT TOXOPLASMOSIS ON AUTOIMMUNE THYROID DISEASES IN PREGNANT WOMEN - DUHOK CITY-KURDISTAN REGION- IRAQ

Background: Toxoplasmosis is a common zoonotic disease has a global distribution and can infect many hosts causes several clinical symptoms in humans and attack many body organs leading to hormonal and behavioral alterations in infected hosts. Latent or dormant form toxoplasmosis is the common form that can affect pregnancy course. Autoimmune thyroid disease (AITD) belongs to the known risk factors for adverse pregnancy outcomes. The aim of this study was the detection of anti-Toxoplasma antibodies and its effects on autoimmune thyroid disease among pregnant women.

Impact of Toxoplasmosis in Im-mune Respons in Hemodialysis Patients

Background:Toxoplasma gondii is the most common infection of protozoa, affecting a wide range of hosts. Toxoplasmosis is usually asymptomatic in immu-nocompetent persons, but in immunocompromised persons (ex:patients on dialysis) significant problems can ensure, and may progress to a life-threatening infection. Objective: To investigate the seroprevalence of anti-Toxoplasma anti-bodies in patients undergoing hemodialysis in Baquba / Iraq . Patients and Methods: A group of blood samples, consisting of 75 dial-ysis patients and 50 healthy controls, were examined for the detection of anti-toxoplasma antibodies in Baquba Governorate from November 2020 to April 2021 using enzyme-linked immunosorbent assay (ELISA) for IgG and IgM. Results: The total numbers of the 125 samples were examined, 32 (44.0%) and 2 (4.0%) were IgG-positive for both patients and healthy individual respectively, and the results of the current study showed only 1 (1.33%) was seropositive IgM for patient group, while no seroposi-tive healthy subjects was found for IgM . The results showed that the percentage of seropositivity was higher in 17 males (40.47%) than in females 15 (45.45%) in the group of patients, but the differences were not statistically significant . As for the age groups, a positive increase was observed in the age group 11-30 years when comparing patients and healthy people, as the percentage reached 5 (29.41%). It was also ob-served that the highest seropositivity in the education levels was in the group of patients at the educational level 6-12 (45.45%) than in the healthy controls group, but the differences were not statistically signifi-cant between education levels. Patients living in urban areas had signifi-cantly higher serum (P = 0.0041) than patients in rural areas, and the odds ratio was 22,000. And those who had contact with cats showed a significant increase in seropositivity p = 0.0466 and odds ratio was 20.5789. Conclusion: Dialysis patients are at risk for toxoplasmosis and should be screened on a regular basis to prevent the disease from spreading dur-ing hemodialysis. Keywords: Toxoplasma gondii, Hemodialysis, Antibodies ,IgG

Detection of Anti Toxoplasma antibodies in children with autism in Shebin Al-Kom district Menoufia Governorate, Egypt

Background; Autism spectrum disorders (ASD), are a range of mental disorders of neurodevelopmental type. More than three billion human hosts are estimated to be affected by the protozoan parasite Toxoplasma gondii, which is intracellular pathogen with a particular preference for the central nervous system (CNS). Accumulating evidence suggests that latent chronic toxoplasmosis contributes to triggering and progression of many neurological and psychiatric disorders, however the link and prevalence in ASD is not clear. Objective; The present study aimed to detect the relationship between Toxoplasma gondii infection in children suffering from autism disorder. Method: A case-controlled study was conducted on 80 children (40 cases and 40 controls), aged from 4-10 years. They were tested for the presence of anti-T. gondii. antibodies by ELISA. Analysis of data was performed using the Chi-square test and Fisher’s exact test. Results; No significant differences were detected between healthy (5.0%) and diseased children regarding presence of anti-toxoplasmosis antibodies (17.5%). However a significant difference was observed between boys and girls concerning age and seroprevalence of toxoplasmosis, the percent of autistic boys with toxoplasmosis was 9.1%, while the infection was represented in 57.1% of girls with autism. History of acquiring infection with toxoplasmosis during pregnancy was positive in 27.5% of diseased children compared to control children where the percent was 12.5%, with no statistically significant result. Considering residence, there were no significant differences in the seroprevalence of T. gondii IgG among urban and rural regions (P-value =1.0). Conclusion: From our study, we concluded that despite the absence of significant difference between healthy and diseased children in regard to toxoplasmosis, autistic children were highly risky for catching toxoplasmosis infection which need more research in this era.

Toxoplasmosis: Seroprevalence in pregnant women, and serological and molecular screening in neonatal umbilical cord blood

Toxoplasmosis is a common zoonotic disease that can also be transmitted from the mother to the embryo, with the risk of congenital infection varying around the world. The aim of this study was to screen pregnant women and their neonates for toxoplasmosis by serologic and molecular methods and assess the impact of risk factors associated with toxoplasmosis on the rate of congenital infection.This study was conducted at a regional maternity hospital in Arak, the capital of the Markazi Province in Iran, during a period of six months. All selected pregnant women (n=261) and the corresponding cord blood samples were serologically screened for toxoplasmosis, with seropositive samples also undergoing molecular testing. Demographic data, as well as information related to the risk factors associated with the transmission of the disease, were collected from mothers and their neonates. The detection of anti-Toxoplasma antibodies and the extraction of DNA from blood samples were conducted using commercial kits. Results showed that the sera of 87 maternal blood samples (33.3%) and 40 cord blood samples (15.3%) were positive for anti-Toxoplasma antibodies (IgG and/or IgM). Molecular screening of the seropositive samples only identified one positive cord blood sample. In other words, the diagnosis of congenital toxoplasmosis was definitive in only one neonate. There was no significant association between the risk of parasite transmission and neonatal seropositivity (p >0.05). Therefore, the results showed that the prevalence of congenital toxoplasmosis in the studied area was consistent with the global rate and suggest that the implementation of newborn screening and follow-up testing could help reduce the disease risk.

Toxoplasmosis: The value of molecular methods in diagnosis compared to conventional methods

Zineb Tlamcani, Zohra Lemkhenete, Badre Eddine Lmimouni Toxoplasmosis is a parasitic infection due to Toxoplasma gondii an obligate intracellular protozoan parasite. It is conssiderate one of the most common parasite worldwide. The contamination of the parasite is generally occurred via conssumption of infected food or water or, undercooked contaminated meat. Toxoplasma gondii infection may lead to serisous illness when the organism is contracted while pregnancy or when it is reactivated in immune-suppressed persons. Diagnosis of toxoplasmosis in humans is elaborated using various techniques such as detection of anti-Toxoplasma antibodies, mouse inoculation, histological revelation of tachyzoites in tissue sections or smears of body fluid, but the detection of Toxoplasma gondii DNA by molecular methods has revolutionized prenatal diagnosis of congenital toxosplasmosis and in immunocompromised patients. In this paper we will discuss the parasite and different methods of diagnosis including the usefulness of molecular methods.

Use of the Hemagglutination Test in the Detection of Antitoxoplasma Antibodies in Mouse Serum

Use of the Hemagglutination Test in the Detection of Antitoxoplasma Antibodies in Mouse Serum