Abstract Nanocomposite materials have attracted considerable interest in several disciplines owing to their distinctive characteristics and possible uses. The primary objective of this study was to synthesize and characterize a nanocomposite material consisting of cerium that has been doped with yttrium. The shape, structure, and properties of the synthesized nanocomposite was characterized using various characterization techniques including ultraviolet spectrophotometry, Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), X-ray diffraction (XRD), zeta potential measurements, dynamic light scattering (DLS), and Fourier-transform infrared spectroscopy (FTIR).The antibacterial activity of the nanocomposite was assessed against a range of pathogenic bacteria including Gram-positive bacteria - Enterococcus faecalis, Staphylococcus aureus, and Gram-negative bacteria- Escherichia coli, Pseudomonas aeruginosa using standard microbiological techniques. The results indicate that the nanocomposite possesses strong antibacterial properties against a wide range of microorganisms, showcasing its potential as a highly effective antimicrobial agent for diverse applications in the biological, environmental, and industrial fields. Hemolysis is the process of red blood cell destruction, resulting in the release of hemoglobin into the surrounding fluid. In this study, 0.7:0.3M nanocomposite had showed effective biocompatibility with less than 5%. Furthermore, this particular composite remained identical in different time intervals, while other composites would normally fluctuate. Research has shown that meticulous material design and surface modification can reduce the harmful effects on red blood cells, hence improving the safety of nanocomposite for therapeutic purposes. In summary, this study offers valuable information on the creation, analysis, and assessment of nanocomposite materials with antimicrobial properties.