Delphinidin Derivatives Research Articles

In vitro and in silico study of eggplant extract on human cancer cell lines.

The aim of this research was to study the metabolite composition, antioxidative potential and cytotoxic activities of Solanum melongena fruit extracts. Phytochemical analyses of extracts were performed using LC-MS. Phenolic compounds were the major constituents present in the fruit extracts. Free radical scavenging activities were recorded and the highest activities were reported in methanolic extracts using DPPH (103.70 ± 2.75 EC50 μg/mL), ABTS (81.74 ± 3.64 EC50 μg/mL), and FRAP (22.39 ± 1.52 μmol TE/g) assays. Quantification has suggested the presence of delphinidin derivatives, and caffeic acid conjugates as major constituents of fruit extracts. The potential binding of these derivatives with human cell surface receptors was analysed using in silico analysis and validated for cytotoxic and apoptotic effects using in vitro studies on human cancer cell lines. The methanolic extract has shown the highest cytotoxic activity.

Exposure to Light of the Abaxial versus Adaxial Side of Detached Kalanchoë blossfeldiana Leaves Affects Anthocyanin Content and Composition Differently.

The accumulation and composition of anthocyanins in leaves of Kalanchoë blossfeldiana, detached and kept for five days under natural light conditions, were investigated. The presence of fifteen derivatives of cyanidin, petunidin, and delphinidin was found. Changes in the content of each anthocyanin in the leaves before and after exposure to light on the abaxial (naturally upper) and adaxial (naturally lower) sides of the leaves were compared. When the adaxial side was exposed to light, the anthocyanin contents of the leaves did not change. In contrast, when the abaxial side of detached leaves was exposed to light, there was enhanced accumulation of delphinidin-rhamnoside-glucoside, cyanidin-rhamnoside-glucoside, cyanidin-glucoside-glucoside, and two unknown derivatives of petunidin and delphinidin. Application of methyl jasmonate (JA-Me) on the abaxial side exposed to light inhibited the accumulation of these anthocyanins. This effect could probably be due to the presence of these anthocyanins in the epidermal cells of K. blossfeldiana leaves and was visible in the microscopic view of its cross-section. These anthocyanins were directly exposed to JA-Me, leading to inhibition of their formation and/or accumulation. The lack of significant effects of JA-Me on anthocyanin mono- and tri-glycosides may indicate that they are mainly present in the mesophyll tissue of the leaf.

Floral scents, specialized metabolites and stress-response activities in Heritiera fomes and Bruguiera gymnorrhiza from Sundarban mangrove ecosystem.

Floral biochemistry and stress physiology is an underexplored aspect of mangroves, which should be investigated as part of preservation and restoration efforts. A thriving true mangrove tree (Bruguiera gymnorrhiza (L.) Lamk.) and a threatened mangrove-associate species (Heritiera fomes Buch. Ham.) were studied in the Sundarban region of India for seasonal variations in floral odours, non-volatile phytochemicals, antioxidant enzyme activities, and surface water chemistry in surrounding habitat. Both species were found to exhibit significant differences in floral volatilomes, protein contents, antioxidant enzyme activities, total flavonoids, and total phenolic contents between spring and autumn blooms. The bird-pollinated flowers of B. gymnorrhiza also showed considerable seasonal differences in floral anthocyanin and proline contents, indicating vulnerability of the post-anthesis open flowers to environmental factors. Contrarily to previous findings, B. gymnorrhiza floral bouquet appeared to be enriched in various classes of volatiles - dominated by sulphurous compounds in bud stage and terpenoids in open stage. Floral anthocyanins, contributing to the striking colouration of the calyx, were found to comprise cyanidin and delphinidin derivatives. Other glycosides of cyanidin and delphinidin were detected in H. fomes flowers, contributing to visual guides to potential food rewards for pollinating insects. Floral tissue in H. fomes was found to be protected by densely overlapping layers of stellate trichomes containing sesquiterpenoids as phytoprotectants. Comparison of the two floral species suggested that H. fomes flowering is optimized to oligohaline (but not freshwater) vernal conditions; whereas B. gymnorrhiza blooms are adapted for biologically enriched (including abundant herbivores and microbial growth), mesohaline forest habitats.

Metabolic Patterns of Flavonoid and Its Key Gene Expression Characteristics of Five Cultivars of Tulipa gesneriana during Flower Development

Flower color is one of the most important ornamental traits of tulips (Tulipa gesneriana). Five typical tulip cultivars were selected to identify the flavonoid components and analyze their key gene expression in their tepals. Firstly, after preliminary determination of the pigment type, the flavonoids were identified by UPLC-Q-TOF-MS. A total of 17 anthoxanthins were detected in the five cultivars. The total anthoxanthin content in the white tulip and the red tulip showed a similar decreasing trend, while an increasing trend was observed in the black tulip. Similarly, a total of 13 anthocyanins were detected in five tulip cultivars. The black tulip contained the largest number of anthocyanins, mainly delphinidin derivatives (Dp) and cyanidin derivatives (Cy). The total anthocyanin content (TAC) in the orange, red, and black cultivars was higher than that in the white and yellow cultivars and presented an overall increase trend along with the flower development. TgCHS, TgFLS, TgF3H, TgF3′H, TgF3′5′H, and TgDFR, as key structural genes, were involved in the flavonoid synthesis pathway, and the expression patterns of these genes are basically consistent with the components and accumulation patterns of flavonoids mentioned above. Taken together, the flower color in tulips was closely related to the composition and content of anthocyanins and anthoxanthins, which were indeed regulated by certain key structural genes in the flavonoid pathway.

A systematic analysis of anthocyanins inhibiting human, murine, and equine herpesviruses

BackgroundHerpesviruses are common animal and human pathogens that cause severe health problems in children, immunocompromised patients, and infected animals with a host range from fish to mammals. Anthocyanin-containing plant extracts have been described as potent antivirals, which might cause fewer harmful side effects than direct-acting antivirals. Here, we report that an extract of Aristotelia chilensis (Molina) Stuntz (Elaeocarpaceae) (MBE) with a high content of the anthocyanin delphinidin suppresses lytic replication of equine, murine and human herpesviruses of replication in vitro. MethodsWe treated cultured cells with MBE and purified individual anthocyanins present in the extract to determine the most active compound at different concentrations. We subsequently infected the cultures with human herpesviruses 1 (HSV-1) or 8 (HHV-8), murine cytomegalovirus (CMV), or equine herpesviruses 1 (EHV-1) and determined the number of infected cells and viral infectivity. ResultsMBE inhibited the HSV-1, murine CMV, and EHV-1 by up to 2 orders of magnitude. In the presence of the stabilizing randomly methylated-beta-cyclodextrin, the inhibitory concentration could be lowered significantly. We identified delphinidin as an active antiviral compound and showed that the non-glycosylated delphinidin solved and stabilized with sulfobutylether-beta-cyclodextrin allowed usage of approximately 50 times lower concentrations. ConclusionGlycosylated delphinidin derivatives were identified as active antiviral compounds of MBE. This suggests that plant extracts rich in delphinidin-anthocyanins have potent antiviral properties that could be used in treatment and prevention

Transcriptome analysis reveals the mechanism for blue-light-induced biosynthesis of delphinidin derivatives in harvested purple pepper fruit.

Anthocyanins are the main pigments that affect the color and quality of purple-fruited sweet pepper (Capsicum annuum). Our previous study indicated that blue light can induce anthocyanin accumulation in purple pepper. In view of its underlying mechanism that is unclear, here, anthocyanin content was determined, and transcriptome analysis was performed on pepper fruits harvested from different light treatments. As a result, among the identified anthocyanin metabolites, the levels of delphinidin (Dp) glycosides, including Dp-3-O-rhamnoside, Dp-3-O-rutinoside, and Dp-3-O-glucoside, were highly accumulated in blue-light-treated fruit, which are mainly responsible for the appearance color of purple pepper. Correlation between anthocyanin content and transcriptomic analysis indicated a total of 1,619 upregulated genes were found, of which six structural and 12 transcription factor (TF) genes were involved in the anthocyanin biosynthetic pathway. Structural gene, for instance, CaUFGT as well as TFs such as CaMYC2-like and CaERF113, which were highly expressed under blue light and presented similar expression patterns consistent with Dp glycoside accumulation, may be candidate genes for anthocyanin synthesis in response to blue-light signal.

Multiomics Analysis Reveals the Chemical and Genetic Bases of Pigmented Potato Tuber.

Anthocyanins and carotenoids determine the diversity of potato tuber flesh pigmentation; here, the underlying chemical and genetic bases were elucidated by multiomics analyses. A total of 31 anthocyanins and 30 carotenoids were quantified in five differently pigmented tubers. Cyanidin and pelargonidin derivatives determined the redness, while malvidin, petunidin, and delphinidin derivatives contributed to purpleness. Violaxanthin derivatives determined the light-yellow color, while zeaxanthin and antheraxanthin derivatives further enhanced the deep-yellow deposition. Integrated transcriptome and proteome analyses identified that F3'5'H highly enhanced anthocyanin biosynthesis in purple flesh and was responsible for metabolic divergence between red and purple samples. BCH2 significantly enhanced carotenoid biosynthesis in yellow samples and along with ZEP, NCED1, and CCD1 genes determined metabolic divergence between light and deep-yellow samples. The weighted correlation network analysis constructed a regulatory network revealing the central role of AN1 in regulating anthocyanin biosynthesis, and 10 new transcription factors related to anthocyanin and carotenoid metabolism regulation were identified. Our findings provide targeted genes controlling tuber pigmentation, which will be meaningful for the genetic manipulation of tuber quality improvement.

Exposure to the Endophytic Fungi Regulates the Anthocyanin Profiles in the Post-Veraison Grape Berries of ‘Cabernet Sauvignon’

The potential of endophytes to initiate changes in host secondary metabolism is expected to be applied to improve the biochemical qualities of the crop. Our previous study revealed the significant impacts of fungal endophytes on the biochemical properties and the anthocyanin profiles in grape berries of the local cultivar ‘Rose Honey’ (RH). To validate the effects, our present work further assessed the impacts of the same fungal endophytes on grape berries of the worldwide planted grapevine cultivar ‘Cabernet Sauvignon’ (CS). Consistent with the results of RH, exposure to most of the used endophytic fungi shaped the biochemical traits and anthocyanidin profile of the CS grape berries. Among the detected biochemical traits, the phenylalanine ammonia-lyase (PAL) activity in berries had the strongest response to endophytic fungal exposure, and the fungal strains RH32, RH36, and MDR1 had the greatest biochemical impacts on the grape berries. Interestingly, the most anthocyanidin species were detected in the two grape berry varieties when exposed to fungal strains MDR36 and RH34. In both varieties, the total anthocyanin concentrations were quantitatively promoted by strains RH36, RH44, MDR1, and MDR36, but suppressed by strain RH7. Malvidin derivatives and delphinidin derivatives accounted for the majority of the relative abundance of the total detected anthocyanins in CS berries. The acylation degree of anthocyanins in grape berries was also significantly promoted by exposure to fungal endophytes. In CS grape berries, a seldom-distributed anthocyanidin, pelargonidin-3-O-glucoside, as well as the diglucoside anthocyanidin were detected when exposed to fungal strains as RH32, RH34, RH36, MDR1, MDR4, and MDR36. Overall, the endophytic fungal strains MDR36, RH36, and RH34 have the ability to promote metabolite profiles in both grape varieties. This work confirms the possibility of using certain endophytic fungal strains as a strategy for shaping grape pigmentation in vinification at the post-veraison or post-harvest stages.

Novel insight into anthocyanin metabolism and molecular characterization of its key regulators in Camellia sasanqua.

Flower color is a trait that affects the ornamental value of a plant. Camellia sasanqua is a horticultural plant with rich flower color, but little is known about the regulatory mechanism of color diversity in this plant. Here, the anthocyanin profile of 20 C. sasanqua cultivars revealed and quantified 11 anthocyanin derivatives (five delphinidin-based and six cyanidin-based anthocyanins) for the first time. Cyanidin-3-O-(6-O-(E)-p-coumaroyl)-glucoside was the main contributor to flower base color, and the accumulation of cyanidin and delphinidin derivatives differed in the petals. To further explore the molecular mechanism of color divergence, a transcriptome analysis was performed using C. sasanqua cultivars 'YingYueYe', 'WanXia', 'XueYueHua', and'XiaoMeiGui'. The co-expression network related to differences in delphinidin and cyanidin derivatives accumulation was identified. Eleven candidate genes encoding key enzymes (e.g., F3H, F3'H, and ANS) were involved in anthocyanin biosynthesis. Moreover, 27 transcription factors were screened as regulators of the two types of accumulating anthocyanins. The association was suggested by correlation analysis between the expression levels of the candidate genes and the different camellia cultivars. We concluded that cyanidin and delphinidin derivatives are the major drivers of color diversity in C. sasanqua. This finding provides valuable resources for the study of flower color in C. sasanqua and lays a foundation for genetic modification of anthocyanin biosynthesis.

Transcriptomic analyses reveal light-regulated anthocyanin accumulation in ‘ZhongShan-HongYu’ grape berries

Teinturier grapevine cultivars of grapevine (Vitis vinifera L.) are characterized by red pulp berries. A new teinturier germplasm ‘ZS-HY’, accumulates pigments in its pulp and peel. We observed that bagging treatment (without light) caused an obvious inhibition of anthocyanin accumulation in ‘ZS-HY’ berries. Under bag removal treatment (with light), the malvidin, cyanidin and delphinidin derivatives accumulated rapidly after the berries exposed to light. To further determine the transcriptional mechanism of light-regulated anthocyanin accumulation in ‘ZS-HY’, we performed RNA-sequencing (RNA-seq) on the berries under bagging treatment and bag removal treatment. Correlation between anthocyanin content, concentration and 741 up-regulated differentially expressed genes (DEGs) in transcriptomic data were analyzed by using weighted gene co-expression network analysis (WGCNA), which indicated that the up-regulated genes had a similar expression pattern to the change in anthocyanin. The expression of the VvHY5-like, VvUVR3 and VvBBX25 light-responsive factors; the VvMYBA1, VvMYB30 and VvbHLH61-like transcription factors (TFs); and the VvUDP86A1, VvGT5, VvGT1 and VvUFGT4-like structural genes was significantly increased, under bag removal treatment as compared with the expression levels observed in bagging treatment. The results showed that light plays a critical role in the anthocyanin accumulation of dyer's grapes and in other biological changes. Here, we provide a theoretical basis for the transcriptional regulatory network that operates during light response, which will further strengthen our understanding of the molecular mechanism of light-induced anthocyanin biosynthesis in teinturier grapes.

Comparative transcriptomic analysis identifies KcMYB1 as a R2R3-MYB anthocyanin activator in Kadsura coccinea

Fruit color, as an important appearance attribute, is crucial for attracting consumers. However, the underlying mechanism regulating mature fruit color formation in Kadsura coccinea remains unclear. Here, a comprehensive metabolomics and transcriptomics analysis was performed to investigate the molecular mechanisms of anthocyanin accumulation between two K. coccinea cultivars with different mature fruit colors—‘Dahong No. 1′ (red) and ‘Jinhu’ (yellow). Targeted metabolomic analysis revealed high anthocyanin levels, most of which were cyanidin and delphinidin derivatives, in ‘Dahong No. 1′ mature fruit peel. The SNP analysis indicated that the two different cultivars had similar genetic background. Moreover, comparative transcriptomic analysis demonstrated that differentially expressed genes (DEGs) were related to flavonoid biosynthesis and metabolic process in the two K. coccinea cultivars. Gene expression profiling data showed that the structural and regulatory genes associated with anthocyanin biosynthesis were significantly upregulated in ‘Dahong No. 1′ mature fruit peel, which was verified by quantitative real-time polymerase chain reaction (qRT-PCR). Notably, the key anthocyanin activator KcMYB1 was identified, which was significantly upregulated in ‘Dahong No. 1′ compared with ‘Jinhu’. We further confirmed that KcMYB1 actively regulated the accumulation of anthocyanin by ectopic expression in vivo. Furthermore, allelic constitution of KcMYB1 in K. coccinea were investigated. The present study can provide insights for understanding the regulatory mechanisms of anthocyanin differential accumulation in the mature fruits of K. coccinea.

Genetic mapping, transcriptomic sequencing and metabolic profiling indicated a glutathione S-transferase is responsible for the red-spot-petals in Gossypium arboreum.

A GST for red-spot-petals in Gossypium arboreum was identified as the candidate under the scope of multi-omics approaches. Colored petal spots are correlated with insect pollination efficiency in Gossypium species. However, molecular mechanisms concerning the formation of red spots on Gossypium arboreum flowers remain elusive. In the current study, the Shixiya1-R (SxyR, with red spots) × Shixiya1-W (SxyW, without red spots) segregating population was utilized to determine that the red-spot-petal phenotype was levered by a single dominant locus. This phenotype was expectedly related to the anthocyanin metabolites, wherein the cyanidin and delphinidin derivatives constituted the major partition. Subsequently, this dominant locus was narrowed to a 3.27Mb range on chromosome 7 by genomic resequencing from the two parents and the two segregated progeny bulks that have spotted petals or not. Furthermore, differential expressed genes generated from the two bulks at either of three sequential flower developmental stages that spanning the spot formation were intersected with the annotated ones that allocated to the 3.27Mb interval, which returned eight genes. A glutathione S-transferase-coding gene (Gar07G08900) out of the eight was the only one that exhibited simultaneously differential expression among all three developmental stages, and it was therefore considered to be the probable candidate. Finally, functional validation upon this candidate was achieved by the appearance of scattered petal spots with inhibited expression of Gar07G08900. In conclusion, the current report identified a key gene for the red spotted petal in G. arboreum under the scope of multi-omics approaches, such efforts and embedded molecular resources would benefit future applications underlying the flower color trait in cotton.

Effects of ethephon and low-temperature treatments on blood oranges (Citrus sinensis L. Osbeck): Anthocyanin accumulation and volatile profile changes during storage

Health-promoting anthocyanins in blood oranges can be enriched via appropriate postharvest storage conditions. Here, we explored the changes of anthocyanin accumulation and volatile profiles of Tarocco blood oranges (Citrus sinensis L. Osbeck) treated by the low temperature alone and combined with ethephon (2 mg/mL) during 50 days of storage. The combination treatment of ethephon and low temperature (8 oC) significantly enhanced the levels of total anthocyanins (10.91 ± 0.25 mg/100 g) and individual cyanidin derivatives relative to low-temperature treatment alone. The increases of six cyanidin derivatives and three delphinidin derivatives identified by LC-MS/Q-TOF were consistent with the variation in total anthocyanins. Ethyl butanoate, ethyl 2-methylbutyrate, ethyl hexanoate, ethyl undecanoate, linalool, and d-limonene were identified as main contributors to overall aroma of fresh blood oranges. Volatile compositions and concentrations were decreased under different treatments of ethephon and temperatures, while ethephon could alleviate the loss of activity volatiles during cold storage.

Accumulation of delphinidin derivatives and other bioactive compound in wild maqui under different environmental conditions and fruit ripening stages

There is a growing interest in food and bioactive compounds with health benefits. Several studies on wild fruits have reported high anthocyanin contents and antioxidant potential mainly influenced by abiotic stress, particularly in native berries. Maqui (Aristotelia chilensis (Mol.) Stuntz), a Chilean native berry, has been referred as a “superfruit” due to its phytochemical composition and antioxidant activity. The content of bioactive compounds in wild maqui, specifically delphinidin, is influenced by collection time, genetic traits, and environmental conditions (location). The objective of this study was to evaluate the phenolic content, levels of anthocyanins and flavonols, and antioxidant capacity of maqui at three fruit ripening stages (unripe, preripe and ripe) in three different locations of central Chile (Coihueco, Cayumanque and Tregualemu) for two consecutive seasons (2019–2020 and 2020–2021). There was an interaction (p ≤ 0.05) between location and fruit ripening stage in all the bioactive compounds evaluated. Total polyphenol content varied in accordance with the biosynthesis pathway transformation in the different ripening stages, ranging from 2290 (ripe stage) to 1025 mg gallic acid equivalent 100 g−1 (fresh weight) (unripe stage). An accumulation of delphinidin derivatives was found at the ripe stage, while these compounds accounted for a significant proportion of around 80% of the total anthocyanins in both seasons. The antioxidant capacity varied depending on the season, location, and fruit ripening stage. The highest values were recorded in Cayumanque, with an average value of 8926 µmol Trolox equivalent 100 g−1 (fresh weight) for both seasons. In order to supply raw material of similar quality, which is a key issue for the food and pharmaceutical industries, and allow for sustainable production, seasonal and environmental variations in bioactive compounds as well as chemical characteristics of wild fruit need to be regulated according to edaphoclimatic conditions.

Colorimetric selective quantification of anthocyanins with catechol/pyrogallol moiety in edible plants upon zinc complexation.

Here is examined the colour development from common anthocyanins (i.e., cyanidin, delphinidin, malvidin, and pelargonidin glycosides) and from anthocyanins-rich extracts (i.e., bilberries, strawberries, and raspberries), using zinc-anthocyanin complexes as molecular probe. We have observed the absorbance increase in the blue region in presence of large excess of zinc ion at acidic pH for cyanidin and delphinidin derivatives, likely due to quinoidal base stabilization from catechol and pyrogallol moiety. The assay condition were studied and applied to natural extracts containing these compounds. The same behaviour was observed for bilberry and, to a minor extent, for raspberry extracts, due to the larger cyanidin/delphinidin contents in the former than in the latter. Anthocyanin standard UV-Vis analysis in buffer has shown a very good linear correlation for cyanidin and delphinidin (R2=0.995 and 0.997, respectively), good precision (CV%=7.4% and 5.3% respectively), high sensitivity (Cyε600nm=8300M-1cm-1, LOD=0.264±0.005mgL-1, LOQ=0.478±0.007mgL-1, and Dpε600nm=15,900M-1cm-1, LOD=0.143±0.002mgL-1, LOQ=0.478±0.007mgL-1). The effectiveness of this colorimetric method for the selective quantification of catechol/pyrogallol-based anthocyanins has been demonstrated in the aforementioned complex real matrices and compared to LC-MS/MS analysis and pH-differential method, offering a valuable tool to characterize plant and food extracts particularly rich in zinc-coordinating anthocyanins.

Modifying Anthocyanins Biosynthesis in Tomato Hairy Roots: A Test Bed for Plant Resistance to Ionizing Radiation and Antioxidant Properties in Space.

Gene expression manipulation of specific metabolic pathways can be used to obtain bioaccumulation of valuable molecules and desired quality traits in plants. A single-gene approach to impact different traits would be greatly desirable in agrospace applications, where several aspects of plant physiology can be affected, influencing growth. In this work, MicroTom hairy root cultures expressing a MYB-like transcription factor that regulates the biosynthesis of anthocyanins in Petunia hybrida (PhAN4), were considered as a testbed for bio-fortified tomato whole plants aimed at agrospace applications. Ectopic expression of PhAN4 promoted biosynthesis of anthocyanins, allowing to profile 5 major derivatives of delphinidin and petunidin together with pelargonidin and malvidin-based anthocyanins, unusual in tomato. Consistent with PhAN4 features, transcriptomic profiling indicated upregulation of genes correlated to anthocyanin biosynthesis. Interestingly, a transcriptome reprogramming oriented to positive regulation of cell response to biotic, abiotic, and redox stimuli was evidenced. PhAN4 hairy root cultures showed the significant capability to counteract reactive oxygen species (ROS) accumulation and protein misfolding upon high-dose gamma irradiation, which is among the most potent pro-oxidant stress that can be encountered in space. These results may have significance in the engineering of whole tomato plants that can benefit space agriculture.

Anthocyanin profile of Galician endangered varieties. A tool for varietal selection

Polyphenolic characterization of minority red grapevine varieties is a key factor to improve varietal recovery and promotion. This work focuses on determining phenolic family content and anthocyanin (AN) profiles of 28 different red grape varieties during 2018 and 2019 vintages in Galicia (Northwest of the Iberian Peninsula). Most of these varieties are considered “minority varieties” in this region. Additionally, the relationships between the AN groups of compounds were calculated. AN, flavanols, flavonols, hydroxycinnamic acids and stilbenes were extracted, identified, and quantified by High Performance Liquid Chromatography (HPLC). The results show that the phenolic, and more specifically the AN profile, are useful elements for varietal classification. ‘Castañal’, ‘Ferrón’, ‘Espadeiro’ and ‘Sousón’ stood out because of their content in AN and copigment substances. These varieties differed from the rest of the grape varieties under analysis due to their higher percentages of petunidin and delphinidin derivatives forms. They also had acceptable values of titratable acidity. Since these minority varieties present highly appreciated polyphenolic and acidity characteristics, they should be recommended to be grown in Galicia.

Physiological and molecular response of colored wheat seedlings against phosphate deficiency is linked to accumulation of distinct anthocyanins

Anthocyanin rich colored wheat with additional health benefits has created interest among breeders, consumers and policy makers to address the prevailing malnutrition in the vulnerable population. Researchers are exploring how colored wheat could perform under different nutrient conditions for the maintenance of growth and development. The present study was aimed to investigate the differential response of phosphorous (Pi) deficiency at the seedling stage using hydroponics. Our results showed that Pi-deficiency triggered typical response in the wheat along with the changes in the plant root morphology, total biomass, micronutrient concentration and distinct anthocyanin accumulation. Our physiological and biochemical data revealed that these parameters were positively altered under stress in the colored wheat and the adaptation followed the trend of white < blue <purple < black. Our results also confirmed that stress induced accumulation of distinct anthocyanins including derivatives of cyanidin, delphinidin and peonidin in a genotype dependent manner. Differential expression pattern visualized for the transcripts encoding phosphate transporters, anthocyanin biosynthesis, putative transporters and regulators may be one of the underlying factors. Altogether, our data showed that the black wheat genotype with highest anthocyanin content could able to adapt better with the P stress. This study will help in identifying suitable colored wheat adapting the stress condition and have potential for influence on the future agricultural cultivation practices.

Anthocyanins From Clitoria ternatea Flower: Biosynthesis, Extraction, Stability, Antioxidant Activity, and Applications.

Clitoria ternatea plant is commonly grown as an ornamental plant and possesses great medicinal value. Its flower is edible and also known as blue pea or butterfly pea flower. The unique feature of anthocyanins present in blue pea flowers is the high abundance of polyacylated anthocyanins known as ternatins. Ternatins are polyacylated derivatives of delphinidin 3,3′,5′-triglucoside. This review covers the biosynthesis, extraction, stability, antioxidant activity, and applications of anthocyanins from Clitoria ternatea flower. Hot water extraction of dried or fresh petals of blue pea flower could be employed successfully to extract anthocyanins from blue pea flower for food application. Blue pea flower anthocyanins showed good thermal and storage stability, but less photostability. Blue pea flower anthocyanins also showed an intense blue colour in acidic pH between pH 3.2 to pH 5.2. Blue pea flower anthocyanin extracts demonstrate significant in vitro and cellular antioxidant activities. Blue pea flower anthocyanins could be used as a blue food colourant in acidic and neutral foods. The incorporation of blue pea flower anthocyanins in food increased the functional properties of food such as antioxidant and antimicrobial properties. Blue pea flower anthocyanins have also been used in intelligent packaging. A comparison of blue pea flower anthocyanins with two other natural blue colouring agents used in the food industry, spirulina or phycocyanin and genipin-derived pigments is also covered. Anthocyanins from blue pea flowers are promising natural blue food colouring agent.

Berry Quality and Anthocyanin Content of 'Consort' Black Currants Grown under Artificial Shade.

The effect of artificial shade on berry quality parameters for the field-grown black currant cultivar ‘Consort’ were investigated over two growing seasons in Urbana, Illinois. Four shade treatments reduced photosynthetically active radiation (PAR) from 37 to 83%. Shade had no effect on soluble solids in up to 65% PAR reduction but decreased 11% in 83% shade in one of two years. Shade increased titratable acidity up to 23% in both years. The effect of shade on anthocyanin content revealed greater variation between years than treatments. Shade influence on anthocyanin content was only observed in 2017, when cyanidin derivatives decreased 13–14% from open-sun to 83% shade. Shade did not affect delphinidin derivatives in either year. Environmental factors other than artificial shade may impact black currant berry quality in an understory environment. The results of our study indicate that black currants can maintain berry quality with PAR reductions up to 65%, but some berry quality parameters may decrease when PAR reductions exceed 65% of full sun.