Aim: To comparatively evaluate various Intra-Orifice barriers (IOB) namely; Cention N (Ivoclar Vivadent), Biodentine (Septodont), Light Cured GIC (GC Gold Label 2LC) and Tetric N Flow (Ivoclar Vivadent) for their effects on the microleakage of endodontically treated teeth.
 Study Design: In-vitro study.
 Place of Study: Department of Endodontics and conservative dentistry, Himachal dental college, Sundarnagar, Himachal Pradesh.
 Materials and Methods: Seventy-five mandibular premolars were decoronated to a standardized length, prepared and obturated with Gutta-Percha and AH Plus sealer. Except for control specimens, the coronal 3-mm gutta-percha was removed and filled with different Intra-orifice barrier materials. The specimens (75) were divided into five groups (n = 15) on the basis of the Intra-Orifice barrier material used. Group 1: Control, Group 2: Cention N, Group 3: Biodentine, Group 4: Light Cured GIC, Group 5: Tetric N Flow. In experimental groups, 3 mm of coronal gutta-percha was removed and replaced with the study material. Samples were submerged in 2% methylene blue dye in vacuum for one week. Specimens were longitudinally sectioned and leakage measured using a 50X stereomicroscope and graded for depth of leakage.
 Results: In the present study microleakage of roots were significantly affected by the type of Intra-Orifice barrier used and the following pattern was observed: Tetric N Flow > Biodentine >Light Cured GIC > Cention N>Control.
 Conclusion: The present study concluded that Intra-Orifice barrier placement provides better coronal seal, prevents microleakage, and enhance the longevity of postobturation restorations, Tetric N Flow proved to be the best followed by Biodentine, Light Cured GIC and Control group.