Department Of Bioscience Research Articles

Isolation and Characterization of Plant Growth Promoting Bacteria from Root Nodules of Cicer arientinum L.

Aims: The aim of the present study is to isolate and characterize root nodulating bacteria from Cicer arientinum L cv. GNG 1958 and assess its plant growth promoting properties. The study is based on previous reports that native symbiotic bacteria may be replaced by better performing strains by corss-inoculation to increase productivity and tolarance to Biotic and abiotic stresses.
 Study Design: Isolation of symbiotic bacteria, its biochemical and molecular characterization and assessment of plant growth promoting property through ammonia production, phosphate solubilization and HCN production.
 Place and Duration of Study: Department of Bioscience and Biotechnology, Banasthali Vidyapith, Rajasthan, during 2019-2022.
 Methodology: Root nodulating bacterial strain was isolated from C. arientinum L. cv. GNG 1958 grown at the agricultural fields' located at the Krishi Vigyan Kendra, Banasthali Vidypapith, Rajasthan. Morphological characteristics of the isolates were noted following Bergey’s Manual of Systemic Bacteriology and biochemical tests performed as per standard methods. Molecular characterization of the potential symbiont was performed by PCR of 16s rRNA genes and the PCR product was sequenced. Plant growth promoting property was determined through ammonia production, phosphate solubilization and HCN production.
 Results: Root nodulating bacterial strain was isolated from C. arientinum L.cv. GNG 1958. Out of eighteen isolates, BVCA-58 grew fast and changed the color of BTB dye in media from green to yellow and deep yellow within 2 days of incubation. White colonies were observed when BVCA-58 was inoculated on YEMA media supplemented with Congo red. BVCA-58 has the ability to produce hydrogen cyanide, ammonia and solubilize phosphate. Molecular study of BVCA-58 using 16f-16r and 63f-1244r primers showed 99.89% and 99.62% of similarity with Mesorhizobium jarvisii ATCC-33669 and Mesorhizobium erdmanii USDA-3471 respectively on performing NCBI Blast. BVCA-58 was submitted to NCBI portal as Mesorhizobium sp. strain BVCA-58 with accession nos. OP646813 and OP646810.
 Conclusion: The present study revealed that isolated strain from Cicer arientinum GNG 1958 variety was Mesorhizobium sp. which has potent plant growth promoting traits.

Member's Plaza: Laboratory of Functional Plant Physiology Department of Applied Life Sciences Faculty of Agriculture, Shizuoka University/Plant Nutrition Laboratory, Department of Bioscience and Biotechnology, Kyushu University

Member's Plaza: Laboratory of Functional Plant Physiology Department of Applied Life Sciences Faculty of Agriculture, Shizuoka University/Plant Nutrition Laboratory, Department of Bioscience and Biotechnology, Kyushu University

Influence of Arbuscular Mycorrhizal Fungi (Glomus clarum) and Compost on Early Growth Response of Parkia biglobosa under a Greenhouse Condition

Background and Objective: Arbuscular Mycorrhizal Fungi (AMF) plays a role in the structural stability of soil which governs most soil activities. Stable organic manure such as compost may provide a suitable habitat and energy source for mycorrhizal growth, which is also a benefits in view of soil productivity. The impact of a combination of compost and mycorrhizal on plant growth was assessed in this study. Hence, experiment was conducted to investigate the influence of compost and mycorrhizalon the early growth response of Parkia biglobosa under a greenhouse condition.
 Materials and Methods: In the greenhouse of the Department of Bioscience, Forestry Research Institute of Nigeria. A 2 × 5 factorial experiment in a complete randomized design was conducted; two levels of mycorrhizal (with and without); five levels of compost (10t/ha, 20t/ha, 30t/ha, 40t/ha and no amendments) in two (2) kilogram soil under four (4) replications was set up. Laboratory analyses of soil and organic amendment incorporated in the soil were done. Data on growth variables were taken fortnightly. The data was statistically analyzed and mean were separated using Duncan Multiple Range Test (DMRT).
 Results: The results showed that, there was significant difference (p<0.05) in the plant height of Parkia biglobosa between mycorrhizal and non-mycorrhizal plants across all weeks after transplanting (WAT),compost application with the interaction of AMF at 40 t/harecorded the highest plant height and number of leaves at 16WAT with 35.14 cm and 29.75 respectively, which are relatively comparable to other treatments used, the least plant height and number of leaves were observed when -AMF 0 t/ha ( control) was used as an amendment with 23.00 cm and AMF 0t/ha (15.05) respectively. For collar diameter, all the treatments were comparable to one another except + AMF 30t/ha which produced the lowest collar diameter with mean value of 2.97mm.
 Conclusion: Based on the description of results above, it can be concluded that: The Inoculation of the AMF and compost significantly affect the early growth performance of Parkia biglobosa, thus providing optimum soil physical conditions for it growth.

AN ASSESSMENT OF HEAVY METAL ACCUMULATION CAPACITY OF FIVE AQUATIC MACROPHYTES AND BIOCHEMICAL RESPONSE.

18Oct 2017 AN ASSESSMENT OF HEAVY METAL ACCUMULATION CAPACITY OF FIVE AQUATIC MACROPHYTES AND BIOCHEMICAL RESPONSE. Gaya K.S , Lizzy Mathew and Ramesh Babu M. G. Research ScholarBharathiar University, Coimbatore. Associate professor, Department of Botany, St. Teresas College, Ernakulam . Department of Bio Science, SngistAsc, North Paravur, Ernakulam.v

Erratum to: Titanium scaffold osteogenesis in healthy and osteoporotic rats is improved by the use of low-level laser therapy (GaAlAs).

Department of Bioscience and Oral Diagnosis Institute of Science and Technology Univ Estadual Paulista (UNESP), Av. Engenheiro Francisco Jose Longo, 777

Antibacterial properties of retainers with silver nanoparticles

We read with interest the article entitled “Streptococcus mutans counts in patients wearing removable retainers with silver nanoparticles vs those wearing conventional retainers: A randomized clinical trial” in the February issue of the Journal.1Farhadian N. Usefi Mashoof R. Khanizadeh S. Ghaderi E. Farhadian M. Miresmaeili A. Streptococcus mutans counts in patients wearing removable retainers with silver nanoparticles vs those wearing conventional retainers: a randomized clinical trial.Am J Orthod Dentofacial Orthop. 2016; 149: 155-160Abstract Full Text Full Text PDF Scopus (44) Google Scholar Since we are also working in this area, we had a few questions and would like some explanations, if possible. Regarding the synthesis of silver nanoparticles:•Silver nanoparticles can be synthesized by chemical, physical, and biological methods,2Iravani S. Korbekandi H. Mirmohammadi S.V. Zolfaghari B. Synthesis of silver nanoparticles: chemical, physical and biological methods.Res Pharm Sci. 2014; 9: 385-406Google Scholar and cytotoxicity varies accordingly.3de Lima R. Seabra A.B. Durán N. Silver nanoparticles: a brief review of cytotoxicity and genotoxicity of chemically and biogenically synthesized nanoparticles.J Appl Toxicol. 2012; 32: 867-879Crossref Scopus (399) Google Scholar How did the authors synthesize their silver nanoparticles?•Was the antimicrobial resin synthesized in bulk to standardize the amounts of silver nanoparticles in retainers delivered to patients? The cytotoxcity of stored silver nanoparticles is greater as compared with freshly prepared silver nanoparticles.4Kittler S. Greulich C. Diendorf J. Koller M. Epple M. Toxicity of silver nanoparticles increases during storage because of slow dissolution under release of silver ions.Chem Mater. 2010; 22: 4548-4554Crossref Scopus (923) Google Scholar•What was the ratio of polymer to silver nanoparticles? Was the minimum inhibitory concentration (that is, the concentration at which at least 50% of the bacteria are killed) calculated? Regarding the antibacterial properties:•Which species of S mutans was present in McFarland suspensions? The antibacterial effect can vary according to the species (eg, ATCC 25175). Regarding the intervention:•After appliance delivery, were the patients given any oral hygiene instructions or directed to use any mouthwashes? Coexposure of silver nanoparticles and sodium fluoride also affects the cytotoxicity.5Inkielewicz-Stepniak I. Santos-Martinez M.J. Medina C. Radomski M.W. Pharmacological and toxicological effects of co-exposure of human gingival fibroblasts to silver nanoparticles and sodium fluoride.Int J Nanomedicine. 2014; 9: 1677-1687Google Scholar Regarding patient-related outcomes:•The authors claimed that the color of the final appliance, which could be gray to black depending on the concentration of the silver nanoparticles (shown in the Fig), was acceptable to patients. Could colorless silver nanoparticles have been used instead?•Was there any reason to carry out the T2 test after 7 weeks, since the retention period was 1 year? Perhaps the antibacterial activity could have been analyzed at regular intervals, since there could be differences in silver ions released during the long term.•Cytotoxicity to human gingival fibroblasts should have been tested at the first stage before conducting the in-vivo study because silver has been claimed to have not only cytotoxicity but also genotoxicity.6AshaRani P.V. Low Kah Mun G. Hande M.P. Valiyaveettil S. Cytotoxicity and genotoxicity of silver nanoparticles in human cells.ACS Nano. 2008; 3: 279-290Crossref Scopus (2931) Google Scholar The urinalyses and hematologic tests should have been done as mentioned in the Discussion. We acknowledge the scientific inputs of Meryam S. Rizvi, associate professor, and Jahirul Mazumder, PhD student, Department of Bioscience, Jamia Millia Islamia University, Delhi, India, for their critical review of the study by Farhadian et al. Streptococcus mutans counts in patients wearing removable retainers with silver nanoparticles vs those wearing conventional retainers: A randomized clinical trialAmerican Journal of Orthodontics and Dentofacial OrthopedicsVol. 149Issue 2PreviewThe rough surface of Hawley removable appliances provides an environment for plaque accumulation, leading to enamel demineralization. The aim of this study was to evaluate the effect of silver nanoparticles incorporated into acrylic baseplates of orthodontic retainers on Streptococcus mutans colony-forming units. Full-Text PDF Authors' responseAmerican Journal of Orthodontics and Dentofacial OrthopedicsVol. 150Issue 2PreviewWe appreciate being given the opportunity to discuss the details of our study1 and hope the following explanation will be helpful. Full-Text PDF

Pichia pastoris에서 사람 락토페린 N-lobe의 발현과 항균활성

Department of Bioscience and Biotechnology and Protein Research Center of GRRC, Hankuk University of Foreign Studies, Yongin 17035, Republic of Korea(Received September 2, 2015; Accepted September 11, 2015)ABSTRACT: Lactoferrin (LF) is a multifunctional, iron-binding glycoprotein found in physiological secretions of mammals. LF shows antibacterial, antiviral and antifungal activities. In the present study, a gene encoding the N-terminal lobe of human lactofer rin (hLF) was isolated, cloned and expressed in methylotrophic yeast, Pichia pastoris . The recombinant hLF-N (rhLF-N) protein was secreted into the culture medium at the level of 458 μg/ml in 3 L fermentor. The size of purified hLF-N was estimated as 35 kDa when analyzed by SDS-PAGE and western blotting. The rhLF-N was further confirmed by immunodiffusion using the anti-hLF polyclonal antibody. The expression profile analysis by qRT-PCR showed that the relative mRNA expression of rhLF-N was maximal after 2-3 days of methanol induction and reduced gradually at 4 days. The purified rhLF-N showed broad antibacterial activities against the pathogens such as Staphylococcus aureus, E. coli, Pseudomonas aeruginosa, Burkholderia cepacia, and Salmonella typhimurium. However, rhLF-N showed relatively lower activity when compared to peptides derived from LF. In spite of this weak activity, the rhLF-N expressed in P. pastoris might be more advantageous for the industrial application, because rhLF-N is secreted into the culture medium and the production can also be increased by optimization of culture conditions.Key words: Pichia pastoris, antibacterial activity, expression, human lactoferrin, N-lobe

D(GC)n repeats form Z-DNA within promoter region and repress the promoter activity in Escherichia coli.

d(GC)n repeats form Z-DNA within promoter region and repress the promoter activity in Escherichia coli Shenghe Huang1, Chuxin Wu2, Dongming Li1, Haizhou Wang3, Zechang Rao1, Qiling Shen1, Chunxiang Chen1, Yong Liu3, Xun Xu1, and Chengyu Hu3,* Fuzhou Medical College, Nanchang University, Fuzhou 344000, China, Nanchang Teachers College, Nanchang 330103, China, and Department of Bioscience, College of Life Science, Nanchang University, Nanchang 330031, China

나노기포 산소수 및 수소수가 미세조류 배양에 미치는 영향

* Department of Bioscience and Biotechnology, College of Medical and Life Science, Silla University, Busan 617-736, Korea **Department of Pharmaceutical Engineering, College of Medical and Life Science, Silla University, Busan 617-736, Korea ***Research Division for Industry & Environment, Korea Atomic Energy Research Institute, Daejon 305-353, Korea (Received April 4, 2014; Revised April 20, 2014; Accepted April 29, 2014)

New SNP markers reveal largely concordant clinal variation across the hybrid zone between Mytilus spp. in the Baltic Sea

AB Aquatic Biology Contact the journal Twitter RSS Mailing List Subscribe to our mailing list via Mailchimp HomeLatest VolumeAbout the JournalEditorsTheme Sections AB 21:25-36 (2014) - DOI: https://doi.org/10.3354/ab00566 New SNP markers reveal largely concordant clinal variation across the hybrid zone between Mytilus spp. in the Baltic Sea Małgorzata Zbawicka1,*, Tomasz Sańko1, Jakob Strand2, Roman Wenne1 1Institute of Oceanology PAS, Powstańców Warszawy 55, 81-712 Sopot, Poland 2Arhus University, Department of Bioscience, 4000 Roskilde, Denmark *Corresponding author: mzbawicka@iopan.gda.pl ABSTRACT: Environmental conditions such as a pronounced salinity gradient and postglacial history make the Baltic Sea a suitable area for studying how selection and gene flow affect genetic differentiation in marine species. A cDNA library was used to identify new single nucleotide polymorphisms (SNPs) in Baltic populations of Mytilus spp. mussels. Sixty polymorphic SNPs were used to genotype 642 individual mussels from the inner Baltic, Danish Straits, northwest Denmark and a population of the northeast Pacific. We characterized 49 novel SNP markers that differentiate the populations of the North and Baltic Sea areas. Concordant narrow clines were observed at the entrance of the Baltic Sea for most of these markers. Considerable variance of hybrid index scores was observed in populations with intermediate allele frequencies within the hybrid zone, e.g. in Gedser and Hjelm. The presented results are in accordance with the existence of strong reproductive isolation, probably caused by a combination of exogenous (e.g. adaptation to brackish waters) and endogenous pre- and post-zygotic factors (e.g. selection against hybrids). The overwhelming majority of new SNPs markers showed a larger representation of M. trossulus than M. edulis genes in the nuclear DNA of Baltic Mytilus species. Finally, we identified a few markers with an elevated level of introgression of M. edulis alleles in the Baltic Sea M. trossulus populations in comparison to the reference M. trossulus population of the Pacific. KEY WORDS: Population structure · Hybrid index score · Single locus clines · Diagnostic markers Full text in pdf format Supplementary material PreviousNextCite this article as: Zbawicka M, Sańko T, Strand J, Wenne R (2014) New SNP markers reveal largely concordant clinal variation across the hybrid zone between Mytilus spp. in the Baltic Sea. Aquat Biol 21:25-36. https://doi.org/10.3354/ab00566 Export citation RSS - Facebook - Tweet - linkedIn Cited by Published in AB Vol. 21, No. 1. Online publication date: June 03, 2014 Print ISSN: 1864-7782; Online ISSN: 1864-7790 Copyright © 2014 Inter-Research.

Synthesis of Microbial Cyclosophoraose Derivatives Grafted Magnetic Nanoparticles

Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, KoreaReceived December 11, 2013, Accepted December 24, 2013Key Words : Cyclosophoraoses, Modified cyclosophoraoses, Iron oxide magnetic nanoparticlesRhizobium leguminosarum biovar viciae VF-39 wereisolated from nodules of Vicia faba.

Pesticide drift deposition in hedgerows from multiple spray swaths

1 Aarhus University, Department of Bioscience, Vejlsovej 25, DK-8600 Silkeborg, Denmark 2 Aarhus University, Department of Environmental Sciences, Frederiksborgsvej 399, DK-4000 Roskilde, Denmark 3 Aalborg University, Danish Building Research Institute, A.C. Meyers Vaenge 15, DK-2450 Copenhagen SV, Denmark 4 Institute for Agricultural and Fisheries Research (ILVO), Technology & Food Science Unit-Agricultural Engineering, Belgium

DNA Binding Analysis of Severe Acute Respiratory Syndrome (SARS) Coronavirus Helicase

Department of Bioscience and Biotechnology, Konkuk University, Seoul 143-701, KoreaReceived January 2, 2013, Accepted January 14, 2013Key Words : Severe acute respiratory syndrome coronavirus, Helicase, DNA binding, Filter binding assay,Fluorimetric titration Severe Acute Respiratory Syndrome (SARS) is a lifethreatening disease which has been caused by novel corona-virus, SARS coronavirus (SCV).

Evaluation of vascular wall elasticity of human digital arteries using alternating current-signal photoplethysmography

PurposeA simple method of estimating arterial elasticity in the human finger using a volume-oscillometric technique with photoplethysmography was principally studied under the various effects of age, sex, and cold-stress stimulation for testing the capability of using this technique in arterial elasticity analysis.MethodsAmplitude variations in the alternating current signal of the photoplethysmograph during a continuous change in transmural pressure were analyzed to obtain the blood pressure and the transmural pressure–relative volume difference relationship of the arteries. We first tested the effect of the occluding cuff size on the arterial elasticity analysis in eight subjects (ages 20–45 years) to obtain a suitable cuff size, resulting in the selection of a middle cuff with a 22 mm diameter. Blood pressure and arterial elasticity were measured in six groups of subjects separated into three age-groups of women and men (ages 20–25, 32–45, and over 50 years) for testing the effect of age and sex. Twelve subjects (ages 20–25 years) also had their blood pressure and arterial elasticity measured in three conditions under the influence of the cold-stress stimulation.ResultsAge, sex, and cold-stress stimulation had an impact on mean blood pressure (P < 0.0005, 0.025), whereas pulse pressure and heart rate were statistically unchanged by those factors. Furthermore, an advanced age (over 50 years) was found to induce an increase in relative volume difference values (P < 0.025) and upward shifting of the transmural pressure–relative volume difference relationships, whereas sex, level of mean blood pressure, and cold-stress stimulation had no influence on these forms of the index.ConclusionThis study showed the usefulness of the relative volume difference as being a mean blood pressure-independent indicator for changes in arterial elasticity.

Prediction of genetic interactions using network topology

Gregorio Alanis-Lobato†,‡,*, Carlo V. Cannistraci†,§,*, and Timothy Ravasi†,§! †Integrative Systems Biology Lab, Computational Bioscience Research Centre, King Abdullah University of Science and Technology (KAUST) ‡Department of Computer Science, Mathematical and Computer Sciences and Engineering Division, KAUST §Department of Bioscience, Chemical and Life Science and Engineering Division, KAUST *These authors contributed equally to this work!

Effect of Korean NativeCimicifugaSpecies on Proliferation of Vascular Smooth Muscle Cells

Young-Hee Kang, M.S., Dong-Yun Lee, M.D., Doo Seok Choi, M.D., Byung-Koo Yoon, M.D., Young Joo Lee, Ph.D., Mee-Ra Rhyu, Ph.D. Samsung Biomedical Research Institute, Department of Obstetrics and Gynecology, Samsung Medical Center, Sungkyunkwan University School of Medicine, Department of Bioscience and Biotechnology, Sejong University, Seoul, Divisions of Metabolism and Functionality Research, Korea Food Research Institute, Seongnam, Korea

ChemInform Abstract: Discovery of Novel Inhibitors of Dual‐Specificity Phosphatase Pyst2 with Structure‐Based Virtual Screening.

Department of Bioscience and Biotechnology, Sejong University, Seoul 143-747, Korea Department of Bioengineering and Institute for Bioengineering and Biopharmaceutical Research, Hanyang University, Seoul 133-791, Korea. *E-mail: ryuse@hanyang.ac.kr Medical Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-600, Korea *E-mail: ksj@kribb.re.kr Received March 15, 2011, Accepted May 20, 2011

Barbiturates as Noncompetitive Inhibitors of PTP1B and Competitive Inhibitors of Vaccinia H1-Related (VHR) Phosphatase

Department of Bioscience and Biotechnology, College of Engineering, Sejong University, Seoul 143-747, KoreaReceived March 4, 2011, Accepted April 5, 2011Key Words : Barbiturate, Protein tyrosine phosphatase, Vaccinia H1-related, VHR, PTP1BBarbiturates have a long history of being used as drugs. Sincethe first barbiturate, Veronal, was marketed as a sleeping aid morethan a century ago, barbiturates have been used as central nervoussystem (CNS) depressants.

Discovery of Novel Inhibitors of Dual-Specificity Phosphatase Pyst2 with Structure-Based Virtual Screening

Department of Bioscience and Biotechnology, Sejong University, Seoul 143-747, Korea Department of Bioengineering and Institute for Bioengineering and Biopharmaceutical Research, Hanyang University, Seoul 133-791, Korea. *E-mail: ryuse@hanyang.ac.kr Medical Proteomics Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-600, Korea *E-mail: ksj@kribb.re.kr Received March 15, 2011, Accepted May 20, 2011

Emerging use of nanostructure films containing capped gold nanoparticles in biosensors

The localized surface plasmon resonance (LSPR) property of gold nanoparticles (GNP) has been exploited in a variety of optical sensor configurations including solution-based bioassays, paper-based colorimetric detection, surface-confined nanoparticle film/array-based sensing, etc. Amongst these, gold nanostructured films are of great interest because of their high stability, good reproducibility, robustness, and cost-effectiveness. The inherent optical characteristics of GNP, are attributed to parameters like size and shape (eg, nanospheres, nanorods, nanostars), eg, LSPR spectral location sensitivity to the local environment, composition (eg, gold-silver or silica-gold nanoshells), sensing volume, mesospacing, and multiplexing. These properties allow sensor tunability, enabling enhanced sensitivity and better performance of these biosensors. Ultrasensitive biosensor designs were realized using gold nanostructured films fabricated by bottom-up as well as top-down approaches. In this review, we describe the past, present, and future trends in the development of GNP-LSPR-based sensors, concentrating on both design (fabrication) and application. In the process, we have discussed various combinations of GNP size and shape, substrate, and application domains.