Deoxyhemoglobin Concentration Research Articles

Frequency-domain instrument with custom ASIC for dual-slope near-infrared spectroscopy.

Real-time and non-invasive measurements of tissue concentrations of oxyhemoglobin (HbO2) and deoxyhemoglobin (HbR) are invaluable for research and clinical use. Frequency-domain near-infrared spectroscopy (FD-NIRS) enables non-invasive measurement of these chromophore concentrations in human tissue. We present a small form factor, dual-wavelength, miniaturized FD-NIRS instrument for absolute optical measurements, built around a custom application-specific integrated circuit and a dual-slope/self-calibrating (DS/SC) probe. The modulation frequency is 55MHz, and the heterodyning technique was used for intensity and phase readout, with an acquisition rate of 0.7Hz. The instrument consists of a 14 × 17cm2 printed circuit board (PCB), a Raspberry Pi 4, an STM32G491 microcontroller, and the DS/SC probe. The DS/SC approach enables this instrument to be selective to deeper tissue and conduct absolute measurements without calibration. The instrument was initially validated using a tissue-mimicking solid phantom, and upon confirming its suitability for in vivo, a vascular occlusion experiment on a human subject was conducted. For the phantom experiments, an average of 0.08° phase noise and 0.10% standard deviation over the mean for the intensities was measured at a source-detector distance of 35mm. The absorption and reduced scattering coefficients had average precisions (variation of measurement over time) of 0.5% and 0.9%, respectively, on a window of ten frames. Results from the in vivo experiment yielded the expected increase in HbO2 and HbR concentration for all measurement types tested, namely SC, DS intensity, and DS phase.

Hemodynamics of the Frontopolar and Dorsolateral Pre-Frontal Cortex in People with Multiple Sclerosis During Walking, Cognitive Subtraction, and Cognitive-Motor Dual-Task

Introduction Higher cortical activity has been observed in people with multiple sclerosis (pwMS) during walking and dual-tasking. However, further studies in overground walking and considering pre-frontal cortex (PFC) sub-areas are necessary. Objectives To investigate PFC activity during a cognitive-motor dual-task (DT) and its single component tasks, in combination with behavioral outcomes in pwMS. Methods Fifteen pwMS (EDSS 3.5 [2-5.5], 42 ± 11 years) and 16 healthy controls (HC, 45.2 ± 13.2 years) performed 3 conditions: single motor-walking (SWT), single cognitive – subtracting sevens (SCT), and a DT. Meters walked and the number of correct answers were obtained from which, respectively, the motor (mDTC) and cognitive (cDTC) DT costs were calculated. A functional Near-Infrared Spectroscopy covering the frontopolar and dorsolateral PFC (DLPFC) areas was used to concentration of relative oxyhemoglobin (ΔHbO2) and deoxyhemoglobin (ΔHHb) in the PFC. A repeated 2-way ANOVA (group × conditions) was used to compare ΔHbO2/ΔHHb and behavioral outcomes. Results PwMS walked shorter distances (P < .002) and answered fewer correct numbers (P < .03) than HC in all conditions, while cDTC and mDTC were similar between groups. PwMS presented higher ΔHbO2 in the frontopolar area than HC in the SWT (P < .001). HC increased ΔHbO2 in frontopolar during the SCT (P < .029) and DT (P < .037) compared with the SWT. Conclusion Higher frontopolar activity in pwMS compared to HC in the SWT suggests reduced gait automaticity. Furthermore, it seems that only HC increased neural activity in the frontopolar in the SCT and DT, which might suggest a limit of cognitive resources to respond to DT in pwMS.

Exploration of resting-state brain functional connectivity as preclinical markers for arousal prediction in prolonged disorders of consciousness: A pilot study based on functional near-infrared spectroscopy.

There is no diagnostic assessment procedure with moderate or strong evidence of use, and evidence for current means of treating prolonged disorders of consciousness (pDOC) is sparse. This may be related to the fact that the mechanisms of pDOC have not been studied deeply enough and are not clear enough. Therefore, the aim of this study was to explore the mechanism of pDOC using functional near-infrared spectroscopy (fNIRS) to provide a basis for the treatment of pDOC, as well as to explore preclinical markers for determining the arousal of pDOC patients. Five minutes resting-state data were collected from 10 pDOC patients and 13healthy adults using fNIRS. Based on the concentrations of oxyhemoglobin (HbO) and deoxyhemoglobin (HbR) in the time series, the resting-state cortical brain functional connectivity strengths of the two groups were calculated, and the functional connectivity strengths of homologous and heterologous brain networks were compared at the sensorimotor network (SEN), dorsal attention network (DAN), ventral attention network (VAN), default mode network (DMN), frontoparietal network (FPN), and visual network (VIS) levels. Univariate binary logistic regression analyses were performed on brain networks with statistically significant differences to identify brain networks associated with arousal in pDOC patients. The receiver operating characteristic (ROC) curves were further analyzed to determine the cut-off value of the relevant brain networks to provide clinical biomarkers for the prediction of arousal in pDOC patients. The results showed that the functional connectivity strengths of oxyhemoglobin (HbO)-based SEN∼SEN, VIS∼VIS, DAN∼DAN, DMN∼DMN, SEN∼VIS, SEN∼FPN, SEN∼DAN, SEN∼DMN, VIS∼FPN, VIS∼DAN, VIS∼DMN, HbR-based SEN∼SEN, and SEN∼DAN were significantly reduced in the pDOC group and were factors that could reflect the participants' state of consciousness. The cut-off value of resting-state functional connectivity strength calculated by ROC curve analysis can be used as a potential preclinical marker for predicting the arousal state of subjects. Resting-state functional connectivity strength of cortical networks is significantly reduced in pDOC patients. The cut-off values of resting-state functional connectivity strength are potential preclinical markers for predicting arousal in pDOC patients.

The association between cardiac T2*BOLD and quantitative flow ratio (QFR) in the diagnosis of stenotic coronary arteries in patients with multi-vessel coronary artery disease.

T2*BOLD is based on myocardial deoxyhemoglobin content to reflect the state of myocardial oxygenation. Quantitative flow ratio is a tool for assessing coronary blood flow based on invasive coronary angiography. This study aimed to evaluate the correlation between T2*BOLD and QFR in the diagnosis of stenotic coronary arteries in patients with multi-vessel coronary artery disease. Fifty patients with MVCAD with at least 1 significant coronary artery stenosis (diameter stenosis > 50%) and 21 healthy control subjects underwent coronary angiography combined with QFR measurements and cardiovascular magnetic resonance (CMR). QFR ≤ 0.80 was considered to indicate the presence of hemodynamic obstruction. Totally 60 (54%) obstructive vessels had hemodynamic change. Between stenotic coronary arteries (QFR ≤ 0.8) and normal vessels, T2*BOLD showed AUCs of 0.97, 0.69, and 0.91 for left anterior descending (LAD), left circumflex (LCX) and right coronary (RCA) arteries and PI displayed AUCs of 0.89, 0.77 and 0.90 (all p > 0.05, except for LAD). The AUCs of T2*BOLD between stenotic coronary arteries (QFR > 0.8) and normal vessels were 0.86, 0.72, and 0.85 for LAD, LCX and RCA; while, PI showed AUCs of 0.93, 0.86, and 0.88, respectively (p > 0.05). Moreover, T2*BOLD displayed AUCs of 0.96, 0.74, and 0.91 for coronary arteries as before between coronary arteries with stenosis (QFR ≤ 0.8 and > 0.8), but the mean PI of LAD, LCX and RCA showed no significant differences between them. T2* BOLD and QFR have good correlation in diagnosing stenotic coronary arteries with hemodynamic changes in patients with stable multi-vessel CAD. T2* BOLD is superior to semi-quantitative perfusion imaging in analyzing myocardial ischemia without stress.

Cerebral perfusion metrics calculated directly from a hypoxia-induced step change in deoxyhemoglobin

Resting cerebral perfusion metrics can be calculated from the MRI ΔR2* signal during the first passage of an intravascular bolus of a Gadolinium-based contrast agent (GBCA), or more recently, a transient hypoxia-induced change in the concentration of deoxyhemoglobin ([dOHb]). Conventional analysis follows a proxy process that includes deconvolution of an arterial input function (AIF) in a tracer kinetic model. We hypothesized that the step reduction in magnetic susceptibility accompanying a step decrease in [dOHb] that occurs when a single breath of oxygen terminates a brief episode of lung hypoxia permits direct calculation of relative perfusion metrics. The time course of the ΔR2* signal response enables both the discrimination of blood arrival times and the time course of voxel filling. We calculated the perfusion metrics implied by this step signal change in seven healthy volunteers and compared them to those from conventional analyses of GBCA and dOHb using their AIF and indicator dilution theory. Voxel-wise maps of relative cerebral blood flow and relative cerebral blood volume had a high spatial and magnitude congruence for all three analyses (r > 0.9) and were similar in appearance to published maps. The mean (SD) transit times (s) in grey and white matter respectively for the step response (7.4 (1.1), 8.05 (1.71)) were greater than those for GBCA (2.6 (0.45), 3.54 (0.83)) attributable to the nature of their respective calculation models. In conclusion we believe these calculations of perfusion metrics derived directly from ΔR2* have superior merit to calculations via AIF by virtue of being calculated from a direct signal rather than through a proxy model which encompasses errors inherent in designating an AIF and performing deconvolution calculations.

Maximizing the Reliability and Precision of Measures of Prefrontal Cortical Oxygenation Using Frequency-Domain Near-Infrared Spectroscopy.

Frequency-domain near-infrared spectroscopy (FD-NIRS) has been used for non-invasive assessment of cortical oxygenation since the late 1990s. However, there is limited research demonstrating clinical validity and general reproducibility. To address this limitation, recording duration for adequate validity and within- and between-day reproducibility of prefrontal cortical oxygenation was evaluated. To assess validity, a reverse analysis of 10-min-long measurements (n = 52) at different recording durations (1-10-min) was quantified via coefficients of variation and Bland-Altman plots. To assess within- and between-day within-subject reproducibility, participants (n = 15) completed 2-min measurements twice a day (morning/afternoon) for five consecutive days. While 1-min recordings demonstrated sufficient validity for the assessment of oxygen saturation (StO2) and total hemoglobin concentration (THb), recordings ≥4 min revealed greater clinical utility for oxy- (HbO) and deoxyhemoglobin (HHb) concentration. Females had lower StO2, THb, HbO, and HHb values than males, but variability was approximately equal between sexes. Intraclass correlation coefficients ranged from 0.50-0.96. The minimal detectable change for StO2 was 1.15% (95% CI: 0.336-1.96%) and 3.12 µM for THb (95% CI: 0.915-5.33 µM) for females and 2.75% (95%CI: 0.807-4.70%) for StO2 and 5.51 µM (95%CI: 1.62-9.42 µM) for THb in males. Overall, FD-NIRS demonstrated good levels of between-day reliability. These findings support the application of FD-NIRS in field-based settings and indicate a recording duration of 1 min allows for valid measures; however, data recordings of ≥4 min are recommended when feasible.

Assessment of Microvascular Hemodynamic Adaptations in Finger Flexors of Climbers.

Climbing performance is greatly dependent on the endurance of the finger flexors which, in turn, depends on the ability to deliver and use oxygen within the muscle. Near-infrared spectroscopy (NIRS) and diffuse correlation spectroscopy (DCS) have provided new possibilities to explore these phenomena in the microvascular environment. The aim of the present study was to explore climbing-related microvascular adaptations through the comparison of the oxygen concentration and hemodynamics of the forearm between climbers and non-climber active individuals during a vascular occlusion test (VOT). Seventeen climbers and fifteen non-climbers joined the study. Through NIRS and DCS, the oxyhemoglobin (O2Hb) and deoxyhemoglobin (HHb) concentrations, tissue saturation index (TSI), and blood flow index (BFI) were obtained from the flexor digitorum profundus during the VOT. During the reactive hyperemia, climbers presented greater blood flow slopes (p = 0.043, d = 0.573), as well as greater O2Hb maximum values (p = 0.001, d = 1.263) and HHb minimum values (p = 0.009, d = 0.998), than non-climbers. The superior hemodynamics presented by climbers could indicate potential training-induced structural and functional adaptations that could enhance oxygen transportation to the muscle, and thus enhance muscle endurance and climbing performance.

Comparison of LED- and LASER-based fNIRS technologies to record the human peri‑spinal cord neurovascular response

Recently, functional Near-Infrared Spectroscopy (fNIRS) was applied to obtain, non-invasively, the human peri‑spinal Neuro-Vascular Response (NVR) under a non-noxious electrical stimulation of a peripheral nerve. This method allowed the measurements of changes in the concentration of oxyhemoglobin (O2Hb) and deoxyhemoglobin (HHb) from the peri‑spinal vascular network. However, there is a lack of clarity about the potential differences in perispinal NVR recorded by the different fNIRS technologies currently available. In this work, the two main noninvasive fNIRS technologies were compared, i.e., LED and LASER-based. The recording of the human peri‑spinal NVR induced by non-noxious electrical stimulation of a peripheral nerve was recorded simultaneously at C7 and T10 vertebral levels. The amplitude, rise time, and full width at half maximum duration of the perispinal NVRs were characterized in healthy volunteers and compared between both systems. The main difference was that the LED-based system shows about one order of magnitude higher values of amplitude than the LASER-based system. No statistical differences were found for rise time and for duration parameters (at thoracic level). The comparison of point-to-point wave patterns did not show significant differences between both systems. In conclusion, the peri‑spinal NRV response obtained by different fNIRS technologies was reproducible, and only the amplitude showed differences, probably due to the power of the system which should be considered when assessing the human peri‑spinal vascular network.

Retrieval of chromophore concentration changes in a digital human head model using analytical mean partial pathlengths of photons.

Continuous-wave functional near-infrared spectroscopy has proved to be a valuable tool for assessing hemodynamic activity in the human brain in a non-invasively and inexpensive way. However, most of the current processing/analysis methods assume the head is a homogeneous medium, and hence do not appropriately correct for the signal coming from the scalp. This effect can be reduced by considering light propagation in a layered model of the human head, being the Monte Carlo (MC) simulations the gold standard to this end. However, this implies large computation times and demanding hardware capabilities. In this work, we study the feasibility of replacing the homogeneous model and the MC simulations by means of analytical multilayered models, combining in this way, the speed and simplicity of implementation of the former with the robustness and accuracy of the latter. Oxy- and deoxyhemoglobin (HbO and HbR, respectively) concentration changes were proposed in two different layers of a magnetic resonance imaging (MRI)-based meshed model of the human head, and then these changes were retrieved by means of (i)a typical homogeneous reconstruction and (ii)a theoretical layered reconstruction. Results suggest that the use of analytical models of light propagation in layered models outperforms the results obtained using traditional homogeneous reconstruction algorithms, providing much more accurate results for both, the extra- and the cerebral tissues. We also compare the analytical layered reconstruction with MC-based reconstructions, achieving similar degrees of accuracy, especially in the gray matter layer, but much faster (between 4 and 5 orders of magnitude). We have successfully developed, implemented, and validated a method for retrieving chromophore concentration changes in the human brain, combining the simplicity and speed of the traditional homogeneous reconstruction algorithms with robustness and accuracy much more similar to those provided by MC simulations.

Classification Algorithm for fNIRS-based Brain Signals Using Convolutional Neural Network with Spatiotemporal Feature Extraction Mechanism

Brain Computer Interface (BCI) is a highly promising human–computer interaction method that can utilize brain signals to control external devices. BCI based on functional near-infrared spectroscopy (fNIRS) is considered a relatively new and promising paradigm. fNIRS is a technique of measuring functional changes in cerebral hemodynamics. It detects changes in the hemodynamic activity of the cerebral cortex by measuring oxyhemoglobin and deoxyhemoglobin (HbR) concentrations and inversely predicts the neural activity of the brain. At the present time, Deep learning (DL) methods have not been widely used in fNIRS decoding, and there are fewer studies considering both spatial and temporal dimensions for fNIRS classification. To solve these problems, we proposed an end-to-end hybrid neural network for feature extraction of fNIRS. The method utilizes a spatial–temporal convolutional layer for automatic extraction of temporally valid information and uses a spatial attention mechanism to extract spatially localized information. A temporal convolutional network (TCN) is used to further utilize the temporal information of fNIRS before the fully connected layer. We validated our approach on a publicly available dataset including 29 subjects, including left-hand and right-hand motor imagery (MI), mental arithmetic (MA), and a baseline task. The results show that the method has few training parameters and high accuracy, providing a meaningful reference for BCI development.

Differential impact of heat and hypoxia on dynamic oxygen uptake and deoxyhemoglobin parameters during incremental exhaustive exercise.

Purpose: This study aims to explore the relationship between the dynamic changes in oxygen uptake () and deoxyhemoglobin (HHb) and peripheral fatigue in athletes during incremental exhaustive exercise under different environmental conditions, including high temperature and humidity environment, hypoxic environment, and normal conditions. Methods: 12 male modern pentathlon athletes were recruited and performed incremental exhaustive exercise in three different environments: normal condition (23°C, 45%RH, FiO2 = 21.0%, CON), high temperature and humidity environment (35°C, 70%RH, FiO2 = 21.0%, HOT), and hypoxic environment (23°C, 45%RH, FiO2 = 15.6%, HYP). Gas metabolism data of the athletes were collected, and muscle oxygen saturation (SmO2) and total hemoglobin content in the vastus lateralis muscles (VL) were measured to calculate the deoxyhemoglobin content. Linear and nonlinear function models were used to fit the characteristic parameters of and HHb changes. Results: The results showed that compared to the CON, , , and exercise time were decreased in the HOT and HYP (p < 0.05). and OUES were reduced in the HOT and HYP compared to the CON (p < 0.05). The Gas exchange threshold in the CON corresponded to higher than in the HYP and HOT (p < 0.05). was reduced in the HOT compared to the HYP (p < 0.05). ΔEHHb was higher in the HOT compared to the CON (p < 0.05). ΔEHHb-1 was increased in the HYP compared to the CON (p < 0.05). There was a negative correlation between ΔEHHb and corresponding in the HOT (r = -0.655, p < 0.05), and a negative correlation between ΔEHHb-1 and corresponding in the HYP (r = -0.606, p < 0.05). Conclusion: Incremental exhaustive exercise in hypoxic environment and high temperature and humidity environments inhibits gas exchange and oxygen supply to skeletal muscle tissue in athletes. For athletes, the accelerated deoxygenation response of skeletal muscles during incremental exhaustive exercise in high temperature and humidity environments, as well as the excessive deoxygenation response before BP of deoxyhemoglobin in hypoxic environment, may be contributing factors to peripheral fatigue under different environmental conditions.

Research on the classification of early-stage brain edema by combining intrinsic optical signal imaging and laser speckle contrast imaging.

The early detection and pathological classification of brain edema are very important for symptomatic treatment. The dual-optical imaging system (DOIS) consists of intrinsic optical signal imaging (IOSI) and laser speckle contrast imaging (LSCI), which can acquire cerebral hemodynamic parameters of mice in real-time, including changes of oxygenated hemoglobin concentration ( ), deoxyhemoglobin concentration (ΔCHbR) and relative cerebral blood flow (rCBF) within the field of view. The slope sum of , ΔCHbR and rCBF was proposed to classify vasogenic edema (VE) and cytotoxic edema (CE). The slope sum values in the VE and CE group remain statistically different and the classification results provide higher accuracy of more than 93% for early brain edema detection. In conclusion, the differences of hemodynamic parameters between VE and CE in the early stage were revealed and the method helps in the classification of early brain edema.

Objective Evaluation of Local Muscle Fatigue in Cerebral Palsy Using A Gait Corrector

The participants were seven individuals (age range 7–36 years) whose walking ability ranged from independent to requiring assistance with a cane, walker, etc. The protocol consisted of resting for at least five minutes before starting to walk, wearing a Gait Corrector device, and walking with light and heavy loads based on the participant's rating of perceived exertion (RPE), followed by resting for 10 minutes before the end of the protocol.Near-infrared spectroscopy (NIRS) electrodes were attached bilaterally to the tibialis anterior and lateral gastrocnemius muscles. NIRS was used to measure blood oxygenation dynamics in local muscles from rest before walking to 10 minutes after walking, and evoked electromyography was used to measure the nerve conduction velocity of the peripheral nerves before and during early and late walking.The tone state of the lateral gastrocnemius muscle was measured three times using an evoked potential-testing device: before walking, immediately after heavy-load walking, and 10 minutes after walking. A recording electrode was attached to the abductor digitorum medialis muscle and a reference electrode was attached to the base of the toe. The M and F waves were recorded using tibial nerve stimulation. The average F/M ratio was used as a parameter of the F-wave.Regarding the change in muscle tone, four of the seven patients exhibited no change or a lower mean F/M ratio after walking than before walking, whereas three patients who exhibited resistance to other dorsiflexion movements of the ankle joint had higher values. Blood oxygenation dynamics revealed a greater disparity between oxyhemoglobin and deoxyhemoglobin concentrations after walking in all participants, suggesting local muscle fatigue, the degree of which showed no improvement even after 10 minutes of rest.

Haemodynamic Responses to Spontaneous Neural Activity on the Electroencephalogram in Preterm Infants.

Delta brushes are spontaneous neural activities observed in preterm electroencephalograms (EEGs) and are thought to reflect the activities of subplate neurons in the developing brain. We investigated the haemodynamic responses associated with delta brushes in five preterm infants at two time points (at 33 or 34, and 36weeks of postmenstrual age), using simultaneous EEG-functional near-infrared spectroscopy (NIRS). An automated detection algorithm was developed to identify the brush components of delta brushes in the bipolar EEG envelope; we placed eight EEG electrodes. An eight-channel NIRS device was placed around the head of each infant to measure changes in oxy- and deoxy-haemoglobin (Hb) concentrations. Haemodynamic grand averages were calculated for local brushes in each NIRS channel. We classified the responses into five patterns based on changes in oxy- and deoxy-Hb signals (positive in-phase/anti-phase, negative in-phase/anti-phase, and unclassified) and evaluated the relationship between the locations of NIRS measurements and those of brushes, as well as the haemodynamic response patterns and infant age at the time of recording. In all the 10 recordings, we found that positive responses (oxy-Hb increases) predominated, not only in the corresponding areas but also in remote areas. Particularly, notable responses were observed in the bilateral temporal areas. Among the positive responses, the proportion exhibiting an anti-phase pattern was 12% at 33 to 34weeks of postmenstrual age and 54% at 36weeks of postmenstrual age. Our unexpected finding of remarkable temporal responses to localised neuronal activity supports the hypothesis that the insula is the most strongly interconnected hub in the developing brain.

Effects of Different Gum Hardness on Masseter Muscle Activity During Gum Chewing: An NIRS Oximetry Study.

Gum chewing is used in orofacial therapy to improve oral functions, such as a patient's chewing ability, bite force, tongue pressure, and lip closure strength. However, its effects on masseter muscle oxygen dynamics and muscle activity may vary with the hardness and features of the gum base. Therefore, when considering gum chewing for therapeutic purposes, it is essential to select gum of an appropriate hardness. This study aimed to elucidate the impact of gum hardness differences on masseter muscle tissue oxygen dynamics and muscle activity. We conducted a 120-s gum chewing study using three types of gum with different hardness levels on 11 healthy adult males. Each patient's masseter muscle tissue oxygen dynamics, muscle activity, and heart rate were measured, and the extent of masseter fatigue was assessed using a visual analogue scale (VAS). Per our findings, as gum hardness increased, significant reductions in oxygen saturation (StO2) and significant increases in deoxyhaemoglobin (Deoxy-Hb) concentrations were observed in masseter muscle tissue oxygen dynamics. Likewise, muscle activity, heart rate, and muscle fatigue (according to VAS) also increased significantly as gum hardness increased. The findings of this study reveal that increasing gum base hardness not only affects masseter muscle tissue oxygen dynamics but also increases muscle activity, masseter fatigue, and heart rate. When selecting gum for orofacial therapy involving gum chewing exercises, it is crucial to choose the appropriate gum.

Impaired Neurovascular Coupling and Increased Functional Connectivity in the Frontal Cortex Predict Age-Related Cognitive Dysfunction.

Impaired cerebrovascular function contributes to the genesis of age-related cognitive decline. In this study, the hypothesis is tested that impairments in neurovascular coupling (NVC) responses and brain network function predict cognitive dysfunction in older adults. Cerebromicrovascular and working memory function of healthy young (n = 21, 33.2±7.0 years) and aged (n = 30, 75.9±6.9 years) participants are assessed.To determine NVC responses and functional connectivity (FC) during a working memory (n-back) paradigm, oxy- and deoxyhemoglobin concentration changes from the frontal cortex using functional near-infrared spectroscopy are recorded. NVC responses are significantly impaired during the 2-back task in aged participants, while the frontal networks are characterized by higher local and global connection strength, and dynamic FC (p < 0.05). Both impaired NVC and increased FC correlate with age-related decline in accuracy during the 2-back task. These findings suggest that task-related brain states in older adults require stronger functional connections to compensate for the attenuated NVC responses associated with working memory load.

Intermittent normobaric hypoxia alters substrate partitioning and muscle oxygenation in individuals with obesity: implications for fat burning.

This single-blind, crossover study aimed to measure and evaluate the short-term metabolic responses to continuous and intermittent hypoxic patterns in individuals with obesity. Indirect calorimetry was used to quantify changes in resting metabolic rate (RMR), carbohydrate (CHOox, %CHO), and fat oxidation (FATox, %FAT) in nine individuals with obesity pre and post: 1) breathing normoxic air [normoxic sham control (NS-control)], 2) breathing continuous hypoxia (CH), or 3) breathing intermittent hypoxia (IH). A mean peripheral oxygen saturation ([Formula: see text]) of 80-85% was achieved over a total of 45 min of hypoxia. Throughout each intervention, pulmonary gas exchanges, oxygen consumption (V̇o2) carbon dioxide production (V̇co2), and deoxyhemoglobin concentration (Δ[HHb]) in the vastus lateralis were measured. Both RMR and CHOox measured pre- and postinterventions were unchanged following each treatment: NS-control, CH, or IH (all P > 0.05). Conversely, a significant increase in FATox was evident between pre- and post-IH (+44%, P = 0.048). Although the mean Δ[HHb] values significantly increased during both IH and CH (P < 0.05), the greatest zenith of Δ[HHb] was achieved in IH compared with CH (P = 0.002). Furthermore, there was a positive correlation between Δ[HHb] and the shift in FATox measured pre- and postintervention. It is suggested that during IH, the increased bouts of muscle hypoxia, revealed by elevated Δ[HHb], coupled with cyclic periods of excess posthypoxia oxygen consumption (EPHOC, inherent to the intermittent pattern) played a significant role in driving the increase in FATox post-IH.

Utilization of Near Infrared Spectroscopy Imaging at Nanoscale for the Identification of Biomarkers in Sports-Induced Muscle Fatigue

This study integrates Near Infrared Spectroscopy (NIRS) and nanoscale imaging technologies to discern alterations in muscle tissue biomarkers, thereby enhancing the precision of non-invasive monitoring of muscle fatigue. Experimental investigations were carried out on the biceps brachii muscle of 12 subjects, categorized into mild, moderate, and severe fatigue groups. Concurrently, a specific wavelength of Near Infrared Laser Diode (NIR-LD) was employed to acquire spectral data. The application of Atomic Force Microscopy (AFM) in conjunction with NIRS imaging facilitated the attainment of high-resolution images of the biceps brachii tissue. The absorption characteristics of distinct biomarkers in muscle tissue, responsive to near-infrared light, were captured to calculate concentration variations and evaluate muscle fatigue levels. The findings revealed substantial variations in the concentrations of Oxy-hemoglobin (HbO), Deoxy-hemoglobin (HbR), Lactic Acid (LA), Phosphocreatine (PCr), Troponin (Tn), Creatine Kinase (CK), and Glutamine (Gln) across different fatigue groups. Muscle fatigue assessment exhibited an average sensitivity, accuracy, specificity, and F1 score of 0.96, 0.96, 0.95, and 0.96, respectively, for the 12 subjects. The average Area Under the Curve (AUC) values for detecting mild, moderate, and severe fatigue were 0.96, 0.96, and 0.98, respectively. This method demonstrates notable accuracy in the identification of muscle fatigue biomarkers, rendering it suitable for sports-related muscle fatigue assessment.

Combining Near-Infrared Spectroscopy and Heart Rate Variability Derived Thresholds to Estimate the Critical Intensity of Exercise.

Fleitas-Paniagua, PR, de Almeida Azevedo, R, Trpcic, M, Murias, JM, and Rogers, B. Combining near-infrared spectroscopy and heart rate variability derived thresholds to estimate the critical intensity of exercise. J Strength Cond Res XX(X): 000-000, 2023-Critical intensity determination often requires costly tools and several testing sessions. Alternative approaches display relatively large individual variation. Therefore, simpler estimations with improved precision are needed. This study evaluated whether averaging the heart rate (HR) and oxygen uptake (V̇O2) responses associated with the muscle deoxyhemoglobin concentration breakpoint ([HHb]BP) and the heart rate variability (HRV) given by the detrended fluctuation analysis second threshold (HRVT2) during ramp incremental (RI) test improved the accuracy of identifying the HR and V̇O2 at the respiratory compensation point (RCP). Ten female and 11 male recreationally trained subjects performed a 15 W·minute-1 RI test. Gas exchange, near-infrared spectroscopy (NIRS), and RR interval were recorded to assess the RCP, [HHb]BP, and HRVT2. Heart rate (mean ± SD: 158 ± 14, 156 ± 13, 160 ± 14 and, 158 ± 12 bpm) and V̇O2 (3.08 ± 0.69, 2.98 ± 0.58, 3.06 ± 0.65, and 3.02 ± 0.60 L·minute-1) at the RCP, [HHb]BP, HRVT2, and HRVT2&[HHb]BP average (H&HAv), respectively, were not significantly different (p > 0.05). The linear relationship between H&HAv and RCP was higher compared with the relationship between [HHb]BP vs RCP and HRVT2 vs RCP for both HR (r = 0.85; r = 0.73; r = 0.79, p > 0.05) and V̇O2 (r = 0.94; r = 0.93; r = 0.91, p > 0.05). Intraclass correlation between RCP, [HHb]BP, HRVT2, and H&HAV was 0.93 for V̇O2 and 0.79 for HR. The [HHb]BP and the HRVT2 independently provided V̇O2 and HR responses that strongly agreed with those at the RCP. Combining [HHb]BP and the HRVT2 resulted in estimations of the V̇O2 and HR at the RCP that displayed smaller variability compared with each modality alone.

Assessing the impact of preferred web app-based music-listening on pain processing at the central nervous level in older black adults with low back pain: An fNIRS study

BackgroundLow back pain (LBP) disproportionately affects older black adults, often leading to inadequate treatment due to clinician biases. Objective pain measures are imperative, and Functional Near-Infrared Spectroscopy (fNIRS) shows promise for pain detection. AimTo determine the impact of listening to home-based preferred web app-based music on underlying pain processing mechanisms at the central nervous level in older black adults aged ≥65 with LBP. MethodsTwenty older black adults with LBP listened to preferred music twice daily for four days using the MUSIC CARE® app. Neuroimaging data were collected using fNIRS. Data were transformed to changes in oxy-hemoglobin and deoxy-hemoglobin concentrations and analyzed. ResultsSignificant cortical activation pattern differences were observed between pre-and post-intervention scans, particularly in somatosensory regions. Post-intervention scans showed significantly reduced hemodynamic activities. ConclusionPreferred music listening has the potential to alleviate pain, and fNIRS emerges as a promising tool for exploring cortical-level pain-related neural circuits.