Background Endodontically treated teeth (ETT) are considered structurally compromised due to significant changes in their biomechanics.The purpose of this in vitro study was to evaluate fracture resistance using three computer-aided design/computer-aided manufacturing (CAD/CAM) customized post systems. Methods Forty extracted intact maxillary central incisors were used in the study, and the teeth were endodontically treated and randomly divided into four equal groups (n = 10): control group without any post and accessed cavity was restored with direct composite restoration. Test groups were prepared for a post and restored according to the material type. The posts and cores were fabricated with computer-aided design/computer-aided manufacturing technology using titanium, vita enamic, and biological dentin materials. The posts were luted using self-adhesive resin cement, and then, zirconia crowns were fabricated, cemented, teeth in all groups were mounted to universal testing machine and subjected to compressive load in Newton at crosshead speed 0.5 mm/min. Results Control group exhibited the highest fracture resistance (727 ± 172.05) followed by biological dentin posts with mean value (712.29 ± 298.58) then group titanium posts with mean value (678.59 ± 132.83) and the least fracture resistance was for group vita enamic with mean value 331.84 ± 74.27. Regarding to fracture favorable it was significantly higher in vita post group than titanium post group (70% vs. 30%) and control group (70% vs. 10%) but it was significantly higher in biological dentin group than titanium group (80% vs. 70%). Conclusion According to type of material, teeth restored with the biological dentin posts showed highest fracture resistance followed by titanium posts, the least fracture resistance was vita enami posts.