Dendrobine and Erianin alleviate inflammation-induced depressive-like behaviors by targeting phosphodiesterase 4B in adolescent mice.

Dendrobine attenuates sepsis-associated acute kidney injury by promoting PINK1/PARKIN-mediated mitophagy.

Enhancing porcine oocyte quality and embryo development through natural antioxidants

Glucocorticoid-induced osteoporosis (GIOP) is the common reason for secondary osteoporosis. Dendrobine (DEN) is the major biologically active component of Dendrobium officinale with anti-inflammatory and antiaging properties. Whether DEN could alleviate osteogenic inhibition in GIOP rats is still unknown. The influence on osteogenic function caused by DEN on dexamethasone-treated bone marrow mesenchymal stem cells and rats was observed. The in vitro results showed that DEN reversed the inhibition of osteogenic differentiation by dexamethasone. Moreover, DEN supplementation attenuated dexamethasone-induced bone loss in vivo. DEN activated JNK and p38 MAPK pathways and restrained GR nuclear translocation, which could be prevented by the JNK (SP600125) or p38 (SB203580) pathway inhibitor. This study verified that DEN alleviated dexamethasone-induced nuclear translocation of GR, and inhibition of osteogenesis via JNK and p38 pathways, laying the foundation for DEN as a therapeutic agent for GIOP.

PPARα is one of the key targets for dendrobine to improve hepatic steatosis in NAFLD

Osteoarthritis (OA) is a degenerative joint disease characterized by low-grade inflammation and cartilage degradation. Dendrobine (DEN) is reported to inhibit inflammation and oxidative stress in some diseases, but its role in chondrocyte senescence and OA progress has not yet been elucidated. Our study aimed to explore the protective effects of DEN on OA both in vitro and in vivo. We found that DEN inhibited extracellular matrix (ECM) degradation and promoted ECM synthesis. Meanwhile, DEN inhibited senescence-associated secretory phenotype (SASP) factors expression and senescence phenotype in IL-1β-treated chondrocytes. Furthermore, DEN improved mitochondrial function and reduced the production of intracellular reactive oxygen species (ROS). Also, DEN suppressed IL-1β-induced activation of the NF-κB pathway. Further, using NAC (ROS inhibitor), we found that DEN might inhibit NF-κB cascades by reducing ROS. Additionally, X-ray, micro-CT, and histological analyses in vivo demonstrated that DEN significantly alleviated cartilage inflammation, ECM degradation, and subchondral alterations in OA progression. In conclusion, DEN inhibits SASP factors expression and senescence phenotype in chondrocytes and alleviated the progression of OA via the ROS/NF-κB axis, which provides innovative strategies for the treatment of OA.

Synthesis of (–)-Dendrobine, (–)-Mubironine B, and (–)-Dendroxine

Synthesis of (-)-Dendrobine

A. Corbella, P. Garibolidi and G. Jommi, J. Chem. Soc., Chem. Commun., 1973, 729 DOI: 10.1039/C39730000729

Abstract cis-4-Carboxy-5-isopropyl-8-methylhydrindane-1,6-dione was synthesized from cis-1-hydroxy-8-methylhydrindanone-5 as a useful intermediate for the synthesis of picrotoxin and dendrobine.

Structure of dendrobine (supplement)

THE STRUCTURE OF DENDROBINE.

Structures of nobiline and dendrobine

1932年,鈴木らによりはじめて石コクからアルカロイドが単離され,デンドロビンと命名された。現在までその構造に関してはほとんど知られていない。著者らは鈴木らと同様な方法で石コクから2種類のアルカロイド(デンドロビン,ノビリン)を得た。従来の化学反応(Hofmann分解,アルカリ分解,酸化,還元など)以外にとくに質量スペクトル,核磁気共鳴吸収スペクトルを活用し,さらにデンドロビンのアセチル化の結果から立体配置をも考慮して,デンドロビンおよびノビリンに対し,それぞれXXI,XXI式を与えることができた。上記アルカロイドの炭素骨核はテルペンにおいてよく知られているイソプレン則にしたがわないが,セスキテルペンに属するピクロトキシニンのそれに完全に一致している。