Delignification Of Wheat Straw Research Articles

RESOURCE-SAVING WHEAT STRAW PROCESSING TECHNOLOGY

The wheat straw delignification condition influence in the medium “acetic acid-water-hydrogen peroxide-citric acid catalyst” to the yield and cellulose product composition was studied. It was established that the fibrous semi-finished product output and the residual lignin content are decrease with increasing temperature and delignification duration. The oxidative wheat straw delignification process was described by a first-order kinetic equation, and it was characterized by low activation energy values of 16.7 kJ mol-1. The oxidized cellulose sample’s structure was studied using IR spectroscopy.

Delignification of Wheat Straw Using DES-like Mixtures

Wheat straw, an agricultural residue, is emerging as a viable alternative to wood biomass alongside other annual plants. The exploration of novel methods, particularly those involving ecofriendly solvents, is at the forefront of harnessing the potential of these annual crops. This study focuses on the delignification of wheat straw using DES-like mixtures composed of choline chloride and lactic acid in a molar ratio of 1:5. We closely monitored three critical factors influencing delignification: temperature, time, and the ratio of biomass to the DES-like mixtures. Temperature ranged from 80 to 160 °C, time varied from 60 to 240 min, and the ratio spanned from 1:10 to 1:60. The percentage of lignin remaining in the samples after delignification varied between 16.13% and 35.49%, while the cellulose content fell within the range of 43.77% to 69.53%. To pinpoint optimal conditions for delignification, we performed calculations. The ideal lignin content of 16.44% was achieved at a temperature of 111 °C, a duration of 60 min, and a biomass-to-solvent ratio of 1:27. For maximum delignification efficiency, reaching 37.32%, the optimal conditions were determined as a temperature of 120 °C, a duration of 150 min, and a ratio of 1:34.

Efficient delignification of wheat straw for microbial lipid production enabled by a novel ternary deep eutectic solvent containing ethylene glycol

Lignocellulose pretreatment often faces the problem of lignin condensation with less attention to biofuel production from total sugars, which significantly hinders biomass fractionation and energy utilization efficiency. The aim of this work was to design a novel ternary deep eutectic solvent (DES) (citric acid: choline chloride: ethylene glycol in a molar ratio of 1:1:2) for pretreatment of wheat straw with a solid/liquid ratio (S/L) of 1:20 (w/w) to achieve non-condensed lignin and high-yield fermentable sugars at 100 °C for 12 h. The introduction of ethylene glycol into ternary DES could capture the formed benzyl carbocation to protect lignin structure, inhibit excessive degradation of hemicellulose but to fermentable sugars (e.g., xylose), and improve the enzymatic digestibility of cellulose (98.73%, w/w). The mechanisms of both lignin fractionation and the in-situ lignin protection during the pretreatment of wheat straw enabled by ternary DES were systematically elucidated. Importantly, Trichosporon cutaneum cultivated in undetoxified DES waste liquid (hemicellulose-rich) and residue hydrolysate (cellulose-rich) could totally afford 8.7 g microbial lipid per 100 g wheat straw, which is superior to previous reports. This green in-situ lignin protection and fractionation strategy provides a new avenue for upgrading lignocellulose to microbial lipids.

Delignification of wheat straw with potassium hydroxide solutions using potassium black liquor as an organomineral fertilizer

При производстве целлюлозы натронным способом из недревесного сырья небольшие предприятия не могут утилизировать черные щелока, так как наличие отдела регенерации экономически оправдано только для крупных целлюлозно-бумажных комбинатов. Эффективно утилизировать отработанные варочные растворы можно при переходе на варку с растворами КОН взамен NaOH и черные щелока использовать как органоминеральное удобрение. Цель исследования - получение целлюлозы из соломы пшеницы с растворами гидроксида калия при различных условиях варки и оценка возможности использования отработанных варочных растворов в качестве органоминерального удобрения. Варки проводили при условиях: гидромодуль 8:1; расход щелочи в ед. К2О и Na2O 10-16% от абсолютно сухого сырья; подъем температуры 60 мин.; варка при температуре 160 С 60 мин. При варке с растворами КОН проводили и изучали стадию пропитки. Из отработанного черного щелока от варки соломы пшеницы с расходом 14% готовили растворы для полива с заданными концентрациями. Дополнительная пропитка дает возможность получить калийную целлюлозу, сопоставимую с натронной по массовой доле лигнина и с меньшей зольностью. Показано, чем выше начальная концентрация варочного раствора при пропитке, тем выше скачок температуры и выше температура при дальнейшей пропитке. Снижение температуры пропитки на 5 С приводит к снижению температуры разогрева суспензии на 0,4-2,3 С, сокращению продолжительности разогрева в 3 раза и ускорению охлаждения системы в среднем в 5 раз. Продолжительность пропитки не более 60 мин. Выявлено, что растворы КОН способствуют лучшему удалению SiO2 из растительного сырья, чем растворы NaOH в заданных условиях варки. Установлено, что отработанный варочный раствор пригоден в качестве органоминерального удобрения с концентрацией 2,8·10-5 г взвешенных веществ в 1 л. In the production of cellulose by the natron method from non-wood raw materials small enterprises cannot dispose of black liquor. It is possible to effectively dispose of used cooking solutions in the transition to cooking with KOH solutions instead of NaOH and black liquor to use as an organomineral fertilizer. The goal of the study was to obtain cellulose from wheat straw with potassium hydroxide solutions under various cooking conditions and to evaluate the possibility of using spent cooking solutions as an organomineral fertilizer. Cooking was carried out under the conditions: hydromodule 8:1; consumption of alkali in units. K2O and Na2O - 10-16% of absolutely dry raw materials; temperature rise 60 min; cooking at 160 C - 60 min. When cooking with KOH solutions, the impregnation stage was carried out and studied. Solutions for irrigation with specified concentrations were prepared from the spent black liquor from cooking wheat straw with a consumption of 14%. Additional impregnation makes it possible to obtain potassium cellulose comparable to sodium in terms of the mass fraction of lignin and with a lower ash content. A decrease in the impregnation temperature by 5 C leads to a decrease in the heating temperature of the suspension by 0.4-2.3 C, a reduction in the heating time by 3 times and an acceleration of cooling of the system by an average of 5 times. It was found that KOH solutions promote better removal of SiO2 from plant raw materials than NaOH solutions under the specified cooking conditions. It was found that the used cooking solution is suitable as an organomineral fertilizer with a concentration of 2.8·10-5 g of suspended solids per liter.

Delignified wheat straw for production of xylo-oligosaccharides and monosaccharides using acetic acid/sodium acetate solution

The production of xylo-oligosaccharides (XOS) from lignocelluloses with conjugated acid-base system is highly efficient. However, XOS production from wheat straw with conjugated system of acetic acid/sodium acetate (HAc/NaAc) has not been reported. In addition, the impact of delignification of wheat straw for XOS production was not clear. In this study, the optimal conditions for HAc/NaAc hydrolysis were: 0.4 M, molar ratio 10:1, 170 °C, and 60 min. The yield of XOS could be rose to 50.2% after the HAc/NaAc hydrolysate was hydrolyzed using xylanase. After 70.3% of lignin in wheat straw was removed by hydrogen peroxide-acetic acid treatment, the XOS yield of delignified wheat straw using HAc/NaAc reached 54.7%. Finally, a glucose yield of 96.6% was gained by cellulase from wheat straw solid. This work suggested that HAc/NaAc hydrolysis could efficiently produce XOS from wheat straw and the delignification of wheat straw was conducive to XOS and monosaccharides production.

Wheat straw delignification by Djerkandera adusta (Willd.) P. Karst. 1879: The effect on enzymatic hydrolysis

The use of lignocellulosic materials in the production of biofuels and biochemicals holds a huge prospect since wood and agricultural residues represent the most abundant global source of renewable biomass. However, delignification is an inevitable step in lignocellulose pre-treatment rendering the cellulose and hemicellulose more exposed to enzymatic saccharification. The aim of this study was to assess the potential of different Bjerkandera adusta strains to enhance the efficiency of enzymatic saccharification of wheat straw after solid-state culturing. Three white-rot fungal strains of Bjerkandera adusta (Willd.) P. Karst. 1879, (BEOFB1601, BEOFB1602 and BEOFB1603) were used for partial delignification of wheat straw during solid-state cultivation. Activity of ligninolytic enzymes were measured spectrophotometrically while wheat straw residues were used for determination of hemicelluloses, cellulose and lignin contents. Enzymatic hydrolysis of pre-treated wheat straw was conducted using commercial cellulase in loadings of 60 U g-1 of solid substrate. The content of reducing sugars was measured calorimetrically using 1,4-dinitrosalycilic acid. Enzymes predominantly responsible for lignin degradation by tested fungal strains were peroxidases. The highest rate of lignin degradation was noticed in samples pretreated with the strain BEOFB1601 (42.3 ? 3.7%). The highest reducing sugars yield (8.6 ? 0.3 gGE L-1) was achieved after enzymatic saccharification of samples pre-treated with the strain BEOFB1601, as the most selective lignin degrader. The obtained results suggest that fungal culturing as a bio?logical pre-treatment method can be significantly strain specific. A key mechanism which enhances convertibility of carbohydrates is selective lignin degradation of the biomass.

Optimization of ultrasound pretreatment and enzymatic hydrolysis of wheat straw: From lab to semi-industrial scale

In this paper, the dominance of the physical effects of ultrasound (US) pretreatment in improving the delignification of wheat straw at a 1:20 solid/liquid (biomass/water) ratio is reported showing low-frequency/high power (25 kHz, 2 or 3 kW) as preferred conditions. The sonochemical pretreatment with alkali was identified as an optimal method to make the cellulose more susceptible to further enzymatic hydrolysis, thereby increasing the yield of soluble sugars. Previous bench-scale studies have shown that US pretreatment has a positive effect on delignification, nevertheless this has not yet been demonstrated on a larger scale. The objective of this study was to demonstrate the effectiveness of US pretreatment for wheat straw delignification from lab to semi-industrial scale (40 kg biomass) in terms of sugar yield and energy efficiency. A 59.2% of lignin removal was recorded after the cavitational treatment of wheat straw with a subsequent 68 ± 5.7% yield in terms of enzymatic hydrolysis (52.6% glucose, 42.9% xylose and 4.5% arabinose).It was demonstrated that the positive effect of US alkali pretreatment of wheat straw on sugar yield was due to a combination of factors, including (i) an easier accessibility of the biomass carbohydrate network to enzymes due to the increased wheat straw porosity, (ii) the partial removal of lignin, which increases the relative proportion of carbohydrates in the treated biomass, and (iii) the disordering (amorphization) of the cellulose structure, which promotes carbohydrate hydrolysis as well as saponification of the acetyl groups in the hemicellulose. With respect to the Life Cycle Assessment (LCA) and the Cost Benefit Analysis the US pretreatment process herein reported was compared to an alternative Steam Explosion pretreatment. The results of the LCA analysis showed, that for all five environmental categories (energy consumption, global warming potential, water and fossil depletion, and the land occupation) the US pretreatment has advantages in comparison with the standard industrial Steam Explosion pretreatment.

Effects of ensiling on Irpex lacteus fermentation in wheat straw: Chemical composition, in vitro rumen digestibility, and fungal community

Ensiling before incubation with Irpex lacteus is effective in controlling spoilage microbes and improving the delignification of wheat straw, however, the interactions between I. lacteus and surviving fungi in silages and their further effects on chemical composition and in vitro rumen digestibility are still unclear. In the present study, 4 % I. lacteus spawn (w/w) was inoculated in untreated (WSI) and ensiled wheat straw (EWSI), and the same substrates without inoculation were set as control and named WS and EWS, respectively. Results show that EWS and EWSI had lower pH than WS and WSI at day 3, but the changing trends of EWS and WSI were opposite in the subsequent fermentation. Although the pH of WSI and EWSI was at an equal level and remained constant from day 7, there were more aerobic bacteria and molds in the former. After 56 days of fermentation, the lignin (sa) content of EWSI decreased by 36 %, and NDS content increased from 292.6 g/kg to 378.1 g/kg. The in vitro rumen dry matter digestibility of EWSI significantly increased by 22 % compared with that of intact wheat straw. The α-diversity of fungal community in all the samples decreased over time, and EWSI had the lowest Shannon index, reflecting only a few fungi dominated the substrates. Several fungi with hemicellulolytic and cellulolytic ability dominated the samples of EWS and WS before day 7, and then microbes with ligninolytic ability, such as Chaetomium, Aspergillus, and Sordaria, replaced them. However, I. lacteus had been the dominant fungal species in WSI and EWSI, though Sordaria and Chaetomium partly replaced it in WSI. The abundance of most fungi in WSI and EWSI decreased in the middle and late stages of fermentation. Negative correlations were observed between I. lacteus and pH, hemicellulose, and lignin (sa), and the relationship with lignin (sa) was further improved by ensiling. Above all, ensiling is an effective method to control unwanted fungi and to improve the delignification and digestibility of wheat straw with I. lacteus.

The effectiveness of the organosolv process in wheat straw delignification optimizing temperature and time reaction

The effectiveness of the organosolv process in wheat straw delignification optimizing temperature and time reaction

Strategic lignin removal from lignocellulosic matrix of wheat straw using selectivity characteristics of ozone

Current study was planned to investigate the ozone selectivity for lignin in wheat straw delignification process under controlled conditions. Operating variables such as ozone concentration, reaction time of ozone with wheat straw and flow rate of ozone were explored and the focus was to prevent depolymerization of cellulose in the lignocellulosic matrix. The novelty in the technique is the strategic use of operating parameters to get maximum lignin removal and least cellulose damage in wheat straw matrix. The removal of lignin was maximum till the first 60 minutes for the ozone flowrates of 4 L min-1, 2 L min-1 and 1 L min-1 of reaction. Further increase in reaction time above 60 min made the process less selective. Results support the idea that the lignin can both sustain and restrict the disintegration of carbohydrates. The flow rate of 2L min-1 with a contact time of 60 min is recommended for optimum removal of 85% of lignin.

HEAT AND MASS EXCHANGE PROCESSES DURING THE DELIGNIIFICATION OF WHEAT STRAW IN THE ROTOR-PULSATION APPARATUS

In the production of biofuel in Ukraine, wheat and sunflower are most widely used. The production of bioethanol from lignocellulosic raw materials (wheat straw) has both a number of advantages and disadvantages compared to traditional raw materials containing starch, due to the complex structure of lignocellulosic biomass. A mandatory stage of raw material processing is its preliminary preparation for hydrolysis. One of the tasks of the research is the delignification of wheat straw and the improvement of the efficiency of the preliminary treatment of lignocellulosic raw materials using the physical effects that occur during the movement of a viscous liquid in rotary-pulsation devices. Wheat straw was used as an object for research. The amount of isolated lignin was determined by the weight method. The results of the experimental studies showed that during the processing of the aqueous dispersion of straw in the rotary-pulsation apparatus, it is heated to a temperature at which intensive release of lignin is ensured. The main mechanism of these processes is the dissipation of the kinetic energy of the rotor rotation. It is shown that the use of rotary-pulsation devices for heating raw materials intended for hydrolysis is an effective substitute for external thermal energy sources. It was found that the treatment of aqueous dispersion of straw in a ratio of 1:10 for 70 min. leads to the release of 42 % of lignin. Changing the hydromodulus from 1:10 to 1:5 increases the percentage yield of lignin to 58 %.

Two C1-oxidizing AA9 lytic polysaccharide monooxygenases from Sordaria brevicollis differ in thermostability, activity, and synergy with cellulase.

Cellulolytic fungi usually have multiple genes for C1-oxidizing auxiliary activity 9 (AA9) lytic polysaccharide monooxygenases (LPMOs) in their genomes, but their potential functional differences are less understood. In this study, two C1-oxidizing AA9 LPMOs, SbLPMO9A and SbLPMO9B, were identified from Sordaria brevicollis, and their differences, particularly in terms of thermostability, reducing agent specificity, and synergy with cellulase, were explored. The two enzymes exhibited weak binding to cellulose and intolerance to hydrogen peroxide. Their oxidative activity was influenced by cellulose crystallinity and surface morphology, and both enzymes tended to oxidize celluloses of lower crystallinity and high surface area. Comparably, SbLPMO9A had much better thermostability than SbLPMO9B, which may be attributed to the presence of a carbohydrate binding module 1 (CBM1)-like sequence at its C-terminus. In addition, the two enzymes exhibited different specificities and responsivities toward electron donors. SbLPMO9A and SbLPMO9B were able to boost the catalytic efficiency of endoglucanase I (EGI) on physically and chemically pretreated substrates but with different degrees of synergy. Substrate- and enzyme-specific synergism was observed by comparing the synergistic action of SbLPMO9A or SbLPMO9B with commercial Celluclast 1.5L on three kinds of cellulosic substrates. On regenerated amorphous cellulose and PFI (Papirindustriens Forskningsinstitut)-fibrillated bleached eucalyptus pulp, SbLPMO9B showed a higher synergistic effect than SbLPMO9A, while on delignified wheat straw, the synergistic effect of SbLPMO9A was higher than that of SbLPMO9B. On account of its excellent thermostability and boosting effect on the enzymatic hydrolysis of delignified wheat straw, SbLPMO9A may have high application potential in biorefineries for lignocellulosic biomass. KEY POINTS: • C1-oxidizing SbLPMO9A displayed higher thermostability than SbLPMO9B, probably due to the presence of a CBM1-like module. • The oxidative activity of the two SbLPMO9s on celluloses increased with decreasing cellulose crystallinity or increasing beating degree. • The two SbLPMO9s boosted the catalytic efficiency of cellulase, but the synergistic effect was substrate- and enzyme-specific.

Two C1-oxidizing lytic polysaccharide monooxygenases from Ceriporiopsis subvermispora enhance the saccharification of wheat straw by a commercial cellulase cocktail

Two genes encoding lytic polysaccharide monooxygenases (LPMOs) CsLPMO9A and CsLPMO9B were cloned from the white-rot fungus Ceriporiopsis subvermispora and successfully expressed in Komagataella phaffii. The oxidative degradation of phosphoric acid swollen cellulose by the LPMOs was conducted using ascorbic acid as an electron donor. Analysis with high performance anion-exchange chromatography and matrix assisted laser desorption/ionization-time-of-flight mass spectrometry indicated that the two enzymes cleaved the polysaccharides by oxidization of the C1 carbon atom of the glucose unit. Synergistic effects between CsLPMO9A or CsLPMO9B and a commercial cellulase cocktail from Trichoderma reesei were observed in the saccharification of delignified wheat straw. The amounts of glucose, cellobiose and xylose released from the substrate by the combined enzymes were increased by 83 %, 16 % and 47 % (for CsLPMO9A), or 79 %, 52 % and 81 % (for CsLPMO9B), compared to the cellulase alone, respectively. The two new enzymes displayed promising potential in industrial bioconversion of lignocellulosic biomass.

Characteristics of flow and heat transfer in rotor-pulsation apparatus during delignification of wheat straw in technology of bioethanol production

Characteristics of flow and heat transfer in rotor-pulsation apparatus during delignification of wheat straw in technology of bioethanol production

Green Deep Eutectic Solvents for Microwave-Assisted Biomass Delignification and Valorisation.

Aiming to fulfil the sustainability criteria of future biorefineries, a novel biomass pretreatment combining natural deep eutectic solvents (NaDESs) and microwave (MW) technology was developed. Results showed that NaDESs have a high potential as green solvents for lignin fractionation/recovery and sugar release in the following enzymatic hydrolysis. A new class of lignin derived NaDESs (LigDESs) was also investigated, showing promising effects in wheat straw delignification. MW irradiation enabled a fast pretreatment under mild condition (120 °C, 30 min). To better understand the interaction of MW with these green solvents, the dielectric properties of NaDESs were investigated. Furthermore, a NaDES using the lignin recovered from biomass pretreatment as hydrogen bond donor was prepared, thus paving the way for a “closed-loop” biorefinery process.

Selective fungal pretreatment favored pyrolysis products of wheat straw based on pyrolytic polygeneration system

A novel wheat straw valorization strategy was developed in which selective fungal pretreatment was applied to promote pyrolytic polygeneration from wheat straw using a fixed-bed reactor. Selective delignification of wheat straw by Pleurotus ostreatus was confirmed. Kinetic analysis based on a modified three-parallel-reaction model indicated that thermally resistant lignin structures were converted to easily degradable structures during fungal pretreatment, facilitating the pyrolysis of wheat straw. The effects of the selective fungal pretreatment on the profiles of pyrolytic products (bio-oil, biogas and biochar) were clearly illustrated. After selective fungal pretreatment, the bio-oil yield increased by 51.09%, and the yields of some high value-added products, such as isosorbide, greatly increased. Moreover, the biochar produced by pyrolysis from the selective fungus-treated wheat straw had characteristics for the adsorption of dyes and pollutants. Therefore, selective delignification by fungal pretreatment increased the thermochemical conversion efficiency during pyrolytic polygeneration from wheat straw.

Pyridinium protic ionic liquids: Effective solvents for delignification of wheat straw

Lignocellulosic biomass covers a vast area of the globe and contains many valuable components that can be envisaged for numerous renewable products. The valorization of these components is hindered by its cumbersome isolation due to the recalcitrant nature of lignocellulosic biomass. Acidic ionic liquids (AILs) have emerged in this field as effective pretreatment solvents. The unique property of disrupting lignin-cellulose-hemicellulose bonds and dissolving lignin makes these ILs significant for the separation of lignin and cellulosic constituents. In this study three protic ionic liquids based on pyridinium cation and hydrogen sulfate anion (HSO4−) have been synthesized; [PyH][HSO4] (IL1), [PyH][HSO4. (H2SO4)] (IL2), and [PyH][HSO4 . (H2SO4)3] (IL3) and utilized for the isolation of lignin from wheat straw. The synthesized ILs were characterized by NMR and FTIR analyses. The lignin yield was optimized with respect to reaction temperature, time, biomass loading, and type of IL. The lignin removal efficacy of the ionic liquid with highest ratio of acid [PyH][HSO4.(H2SO4)3] (IL3) is indicated by high delignification (79%) and lignin recovery (77%) under mild conditions (60 °C, 2 h). The cellulose rich material (CRM) and isolated lignin were characterized by FTIR, SEM, TGA, GPC, and HSQC NMR to assess their structure, molecular weight and stability. The cellulosic part was further hydrolyzed enzymatically to evaluate the capability of the recovered pulp to generate glucose. An appreciable yield of reducing sugars (85%) substantiates high lignin removal.

Effect of introducing ozone in elemental chlorine free bleaching of pulp on generation of chlorophenolic compounds

Abstract Effect of using ozone before elemental chlorine free (ECF) bleaching of oxygen delignified wheat straw pulp produced following soda-anthraquinone pulping on bleaching effluent and pulp properties was studied. The effluent generated during bleaching of pulp contains high amount of adsorbable organic halogens (AOX), chemical oxygen demand (COD), biochemical oxygen demand (BOD) and highly toxic chlorophenolic compounds. This study is aimed to utilise green chemistry approach during bleaching of one of the abundantly used agro residue wheat straw for improving the quality of bleaching effluent and pulp properties. Introducing ozone stage before ECF bleaching resulted in significant reduction of chloriphenolic compounds like chlorocatechols by 48.9 %, chloroguaiacols by 33.3 %, chlorovanillins by 28.4 % and chlorophenols by 26.7 % in the effluent compared to those of control. Incorporation of ozone before ECF bleaching BOD, COD, AOX and colour were reduced by 40.0 %, 41.1 %, 46.7 % and 57.8 %, respectively, as compared to control. Optical properties like brightness and whiteness of the pulp bleached using ozone were also improved by 2.5 units and 4.0 units, respectively as compared to that with control.

Ozonolytic Delignification of Wheat Straw

The dynamics of wheat straw delignification with ozone treatment is studied. The dependence of the residual lignin content on the specific absorption of ozone is determined. It is found that in a prolonged stage of ozonation, ozone is consumed mostly for the reaction with lignin. HPLC is used to record kinetic profiles of water-soluble products of ozonation. The IR spectra of straw samples with various degrees of delignification are analyzed. The results confirm the ozonolytic degradation of lignin and suggest that polysaccharides undergo partial oxidation during prolonged ozonation. A relationship is found between the lignin content and the reactivity of ozone-pretreated straw in enzymatic hydrolysis to sugars. The optimum consumption of ozone is 2 mol-equiv O3 per phenylpropane structural unit (PPU) of the lignin contained in the straw, which corresponds to a residual lignin content of ~10%.

Sorption properties of organosolv lignin towards methylene blue

Involvement of secondary raw materials such as plant wastes and by-products of plant materials processing for solving problems of environmental pollution is considered as a promising direction by scientists in many countries of the world. The purpose of the paper is to study the structure and sorption properties of lignin as a by-product of organosolv delignification of wheat straw. In this work lignin precipitated from the spent cooking solution after oxidative-organosolv delignification of wheat straw was used as the raw material. Different methods of analysis were used to study the structure and properties of the obtained lignin, including gravimetric, spectroscopy, and microscopy. The sorption capacity of the material with respect to methylene blue was studied using model solutions and a spectrophotometric method to determine dye concentrations.Investigation of the sample by the method of infrared spectroscopy and scanning electron microscopy showed that organosolv lignin is a highly dispersed material with particles of various shapes and sizes, and contains a small number of polysaccharides, the total pore volume of such material is 0.431 cm3. The sorption capacity of lignin towards methylene blue was investigated as a function of the pH of the dye aqueous solution, its initial concentration, and the duration of the process. It is shown that the sorption capacity is largely depends on the pH of the medium and increases with a change in pH from 3 to 6. However, a further increase in pH does not change the specified indicator. This dependence of the sorption capacity is related to the property of the surface of the plant material to change the charge from positive in acidic medium to negative in neutral and alkaline, due to the presence of oxygen-containing functional groups (mainly phenolic and aliphatic hydroxyls), which are easy can be protonated and deprotonated. The absorption kinetics of the dye is quite fast and the sorption equilibrium is reached within the first 120 min of contact. Pseudo-first and pseudo-second order models and intraparticle diffusion were used to study the sorption kinetics. Compared to the kinetic model of pseudo-first order (R2 = 0,985) and intraparticle diffusion (R2 = 0,856), the kinetic model of pseudo-second order (R2 = 0,999) better describes the kinetics of sorption and indicates that fixation of the dye on the surface of the material takes place with the involvement of different mechanisms. To study the adsorption equilibrium in the sorption of methylene blue, model solutions with initial dye concentrations of 30 to 500 mg/l were used. It was found that at an initial concentration of methylene blue in a solution below 100 mg/l, the sorption capacity increases linearly with increasing initial concentrations. Then the increase in the sorption capacity is slower. The maximum sorption capacity is 44.7 mg/g. In the case of the adsorption isotherm, the Langmuir equation (R2 = 0.996) is more suitable than the Freundlich equation (R2 = 0.955) to describe the absorption process of the cationic dye and indicates that the adsorption is a monolayer.In general, it was shown that organosolv lignin from wheat straw with sorption capacity slightly exceeds oreganoslv lignin from other plant raw materials. Creating new, inexpensive and effective materials is an urgent task for scientists. Developing new resource-saving technologies to solve the problem of environmental pollution is a priority for chemical technology and ecology worldwide. This is especially important for Ukraine, as efficient processing of agricultural plant waste with the use of environmentally friendly technologies will help to meet the needs of many industries and improve the environment. It is promising for further to study the structure of organosolv lignin to determine the content of various functional groups and to determine the possibility of its use for absorption heavy metal ions from aqueous solutions.