Delay Ripening Research Articles

Application of nanoparticles blue-TiO2-x and chitosan coating to delay ripening and suppress ethylene-related gene expression of Cavendish banana

Cavendish bananas are climacteric fruits that rapidly respond to biochemical reactions during ripening. Therefore, it quickly undergoes a decrease in commercial value during post-harvest storage. This study evaluated the effect of Fruit Storage Chamber (FSC) modification on the photoactivation of nanoparticles blue-TiO2-x and 1.25 % (w/v) chitosan coatings on the physicochemical and molecular characteristics of Cavendish bananas stored at a low temperature (22 °C). The results showed that the combination of FSC treatment supplemented with nanoparticles blue-TiO2-x and bananas coated with 1.25 % (w/v) chitosan can maintain fruit quality during seven days of storage at 22 °C, compared to the control. Applying both of nanoparticles blue-TiO2-x variants with chitosan-coated bananas can suppress the relative expression of the MaACS1 gene until the 5th day but lead to an up-regulated expression on the following day. However, the control sample stored in FSC with nanoparticles blue-TiO2-x was the best treatment to suppress relative MaACS1 (Musa acuminata ACC synthase 1) and MaACO1 (Musa acuminata ACC Oxidase 1) gene expression in the pulp until the end of shelf life. It means the treated sample with 1.25 % chitosan could suppress MaACO1 gene expression six times more than a banana without chitosan while in storage without FSC. MaACO1 expression in nanoparticles blue-TiO2-x bananas coated with 1.25 % (w/v) chitosan decreased 28-fold during storage 7 days. This study suggested that a 1.25 % chitosan coating combined with storage in FSC with nanoparticles blue-TiO2-x can inhibit ACC oxidase from converting ACC to ethylene. This study demonstrated that an FSC equipped with nano-TiO2 variants, low temperature, and 1.25 % (w/v) chitosan coating is a promising solution that could delay ripening and extend the shelf life of Cavendish bananas.

Development of high barrier-coated white cardboard for fruit preservation.

Environment-friendly and biodegradable packaging materials have attracted widespread attention. Development of green solutions to extend the fruit shelf life and address fruit preservation thus has a far-reaching impact. In this study, high-barrier white cardboard (WC) was prepared by a facile coating method. Compared with the WC substrate, the WVP value of the polyvinylidene chloride (PVDC) emulsion-coated WC (3.46 × 10-11 g m m-2 s-1 kPa-1) decreased 73.8% and the OP value (14.8 cm3 m-2 day-1·Pa-1) decreased 61.9%. In addition, the mechanical properties of the PVDC emulsion-coated WC increased significantly. The weight loss rate and decay rate of the stored fruits packaged with PVDC emulsion-coated WC decreased by about 5%. The high barrier PVDC emulsion-coated WC with excellent mechanical properties, good barrier effect, and preservation function was successfully prepared. Benefitting from these investigated characteristics, the obtained coated WC can be used to package fruits to reduce water loss and delay ripening, and thus extend their shelf life, exhibiting a favorable effect on blueberry and grape storage. Overall, the fabricated eco-friendly coated white cardboard has shown great potential for biodegradable packaging applications. We believe the current work presents an approach to address perishable fruit preservation and provide a supplement alternative.

Postharvest Responses of Durian (Durio zibethinus cv. Puyat) Fruit to a Paraffin-based Wax as Surface Coating

Surface coatings have been used to delay deterioration and prolong the shelf-life of perishable crops. In this study, physico-chemical characteristics of ‘Puyat’ durian fruit surface-coated with a paraffin-based wax and held under ambient conditions (25.95 ± 0.91 °C, 90.5 ± 5.0% RH) were evaluated up to 12 d after treatment (DAT). The concentrations used were 0, 10, 15, and 20%. Weight loss was highest in control fruit while visual quality was better in wax-treated fruit at 12 DAT. Dehiscence was delayed by up to 5 d in fruit treated with 20% wax. Pulp firmness was better retained in higher wax concentrations at 4 and 8 DAT. Total soluble solids were lower by up to 44% in all surface-coated fruit. Electrolyte leakage decreased by up to 80% in surface-coated fruit (20%) relative to the untreated lot at 8 DAT. By the end of storage, all treated fruit had greener (lower a*) rinds compared to the untreated lot, hence indicating a delay in ripening. In all treatments, ascorbic acid content declined while total phenolic content was maintained with storage. Thus, the paraffin-based wax could potentially delay the ripening of ‘Puyat’ durian by delaying dehiscence and slowing down weight loss – while not affecting the fruit’s antioxidant potential.

Effects of Delayed Precooling on the Postharvest Quality and Anthracnose Incidence of “Irwin” Mangoes

Mangoes are typical climacteric tropical fruits with accelerated metabolism, degradation, and senescence after harvest due to the accumulation of field heat. Thus, removing field heat as soon as possible after harvest is essential. In addition, a severe postharvest disease of mango, anthracnose, has been reported to be caused by Colletotrichum gloeosporioides, thus decreasing the shelf life and limiting mango export. This study was performed to investigate the effect of delayed precooling on the quality and incidence of anthracnose during storage of Irwin mango at different operational times after the harvest. Therefore, in this study, Irwin was precooled for 30 min at 3, 6, 12, and 24 h postharvest and immediately stored in a cold room at 5°C. The appearance, anthracnose incidence, respiration rate, ethylene production, firmness, total soluble solids (TSS), titratable acidity (TA), and fruit overripening rate were monitored throughout the shelf life. Early precooling was found to delay anthracnose incidence during the shelf life. Furthermore, the fruit respiration rate during the shelf life was negatively correlated with the precooling delay. The respiration rate of fruits precooled for 3 h after harvest was 8.08 mg CO2 kg–1 h–1 and retained a good appearance throughout the shelf life. Their ripening, reduced firmness, and TA were significantly delayed compared with those of fruits with longer precooling delays, which had a firmness of 19 N, TA of 0.29%, and TSS of 12.6°Brix after 30 d of storage at 5°C, while subsequent warming to 20°C for 3 d, the rate of overripening was significantly lower in fruits with precooling delays of 3 and 6 h than in fruits with a precooling delay of 24 h. Thus, precooling mangoes within 3–6 h of harvest will reduce their respiration rate, delay ripening, decrease anthracnose incidence, preserve quality, and prolong storage life.

Dynamic controlled atmosphere monitored by respiratory quotient as a new method to store pears: Effect on the volatile compounds profile and general quality

Pears (Pyrus communis) are climacteric fruit that require storage under appropriate conditions to extend availability and delay ripening. This study evaluated the production of volatile organic compounds (VOCs) and the physicochemical quality of ‘Santa Maria’ and ‘Rocha’ pears after storage in a controlled atmosphere (CA) or dynamic controlled atmosphere (DCA), monitored by respiratory quotient (RQ). The pears, harvested from an orchard in São Joaquim (Santa Catarina State), were stored at − 0.5 °C in CA (2.0 kPa O2) and DCA with RQ levels of 1.1, 1.2, 1.3, and 1.4, all under 1.0 kPa CO2. ‘Santa Maria’ pears were stored for four months, and ‘Rocha’ pears for six months, followed by seven days at 20 °C before quality analysis. Storage of ‘Santa Maria’ pears in CA resulted in fruit with a more yellow skin color, lower flesh firmness, and higher ethylene production, while ‘Rocha’ pears in CA retained higher flesh firmness and produced less ethylene at 4 and 7 days at 20 °C compared to DCA conditions. Storage conditions did not significantly affect soluble solids and acidity. In ‘Rocha’ pears, DCA, regardless of RQ, suppressed the production of butyl acetate, hexyl acetate, pentyl acetate, methyl acetate, and propyl acetate, while in ‘Santa Maria’ pears, esters such as butyl acetate and hexyl acetate were less affected. In conclusion, no significant differences were found between the DCA-RQ levels evaluated in this research in maintaining physicochemical quality and VOCs production. However, DCA suppressed VOCs production in both ‘Santa Maria’ and ‘Rocha’ pears compared with CA storage.

Layer by layer application of chitosan and carboxymethyl cellulose coatings delays ripening of mango fruit by suppressing cell wall polysaccharides disassembly

Mango is a climacteric fruit that ripens quickly after harvest due to its climacteric nature. Edible coatings have been reported to delay the ripening of various harvested fruit. The efficacy of the applied edible coatings could be improved by using in combination as a layer-by-layer (LBL) approach. So, the influence of LBL application of chitosan (CH) and carboxymethyl cellulose (CMC) was studied on mangoes during postharvest storage at 15 °C for 20 days. Mangoes were coated with monolayers of CH (1 % w/v) and CMC (1 % w/v) as well as LBL application of CH and CMC and were compared with control. The treatment of mangoes with CH and CMC-based LBL treatment resulted in lower decay percentage and weight loss along with higher total chlorophyll pigments and suppressed total carotenoid accumulation. The LBL application of CH and CMC showed lower activity of chlorophyll degrading such as chlorophyllase (CPS), pheophytinase (Phe), Mg-dechalatase (MGD) and chlorophyll degrading peroxidase (Chl-POD) enzymes as well as reduced changes in b*, a* and L* along with a suppressed increase in ethylene (EP) and CO2 production (CPR) rates having higher antioxidant such as catalase (CAT), ascorbate peroxidase (APX), peroxidase (POD) and superoxide dismutase (SOD) enzymes activity. In addition, mangoes coated with LBL treatment of CH and CMC exhibited lower water-soluble pectin (WSP) and higher protopectin (PP) having higher concentrations of chelate soluble (CSP) and sodium carbonate-soluble pectin (SCP). Similarly, LBL-coated mangoes showed significantly higher hemicellulose (HCLS) and cellulose (CLS) contents in contrast with control. It was observed that mangoes coated with CH and CMC-based LBL coating exhibited higher flesh firmness and showed suppressed cellulase (CS), pectin methylesterase (PME), polygalacturonase (PG) and β-galactosidase (β-Gal) enzymes activity. The concentrations of total soluble solids and ripening index were markedly lower and titratable acidity was higher in the LBL-based coating treatment in comparison with control. In conclusion, LBL treatment based on CH and CMC coatings could be used for the delay of ripening and softening of harvested mangoes.

Composite Coatings Delay Ripening and Maintain Postharvest Quality in Pear Stored Under Cold and Supermarket Conditions

Composite Coatings Delay Ripening and Maintain Postharvest Quality in Pear Stored Under Cold and Supermarket Conditions

Nutritional and Physiological Effects of Postharvest UV Radiation on Vegetables: A Review.

Effective ultraviolet (UV) irradiation has been used as a postharvest technology to reduce decay, delay ripening, and delay senescence in crop products. In this review, the effects of UV radiation of different wavelengths and doses on physiological and phytochemical parameters in postharvest vegetables are discussed in summary, including appearance (color and texture), microbial load, respiration rate, enzymatic antioxidant system, and various bioactive compounds (phenolic compounds, carotenoids, chlorophylls, vitamins, glucosinolates, betalains, and antioxidant activities). In particular, postharvest UV radiation affects oxidative metabolism and increases the antioxidant activity of plant products, which could help delay yellowing and senescence of vegetables, trigger defense responses, and reduce decay and diseases. In some cases, irradiation stimulates the synthesis of bioactive secondary metabolites that may improve the nutritional value of vegetables. The findings presented in this review are very useful and valuable for the preservation and improvement of the nutritional quality of vegetables after harvest. It will also provide scientific support for industrial and commercial applications of UV radiation in postharvest.

Multifunctional edible chitin nanofibers/ferulic acid composite coating for fruit preservation

AbstractAbout one‐third of the world's entire food production is lost or wasted every year, in which the perishables such as fruits and vegetables account for the largest proportion due to their short shelf life. Therefore, it has attracted great attention to the development of food preservation. In this paper, a simple strategy for food preservation is developed. Chitin nanofiber aqueous suspension is used as the substrate, and ferulic acid (FA) acts as the physical crosslinker to obtain a multifunctional composite protective coating for fruits. The results of Zeta potential, X‐ray photoelectron spectroscopy (XPS) and X‐ray diffraction (XRD) indicate that there are multiple noncovalent interactions between FA and chitin nanofibers. The chitin/FA coating films show superior mechanical properties, water and oxygen resistance. They could effectively reduce the water loss rate, delay ripening, and resist oxidation and microbial invasion for the fresh strawberries and fresh‐cut apples, indicating the potential in food preservation.

Review Article on: 1-Methylcyclopropene (1-MCP) a Breakthrough to Delay Ripening and Extend Shelf-Life of Horticultural Crops

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Use of Sub-Atmospheric Pressure Storage to Improve the Quality and Shelf-Life of Marmande Tomatoes cv. Rojito.

In this study, the feasibility of storing Marmande tomatoes (Solanum lycopersicum, cv Rojito) under hypobaric conditions was evaluated. The fruits were sorted into four lots of 72 fruits each. One lot was considered as a control, and the fruits were kept in the open box, while the fruits of the rest of the three remaining lots were enclosed in airtight containers and subjected to 101, 75 and 50 Kpa, respectively. Control fruits and airtight containers were kept at room temperature, and every three days from the beginning of the experiment the following main quality parameters were analysed: ethylene production rate, firmness, colour, total solids content, ascorbic acid, total phenolics and pigments, as well as a sensory analysis carried out by panellists. The results show that sub-atmospheric storage led a reduction in ethylene production, which was associated with a delay in ripening. The differences in the evolution of pigments were very significant, while a large degradation of chlorophylls was observed in the control fruits and in those kept at 101 kPa, in the fruits kept at 75 kPa and 50 kPa the degradation was much slower. In relation to carotenoid pigments, it was observed that sub-atmospheric treatments delayed their appearance compared to control and 101 kPa fruits. In relation to other quality parameters, it was found that control fruit and fruit held at 101 kPa softened more rapidly than fruit under sub-atmospheric conditions, whose loss of firmness was more gradual with differences found only at 9 and 12 days of storage with respect to fruit firmness at harvest. The appearance of these fruits was evaluated with the same score as at the time of harvesting, during 9 of the 12 days of the experiment, then a positive effect of sub-atmospheric treatments was also found in the sensory analysis. The results suggest that sub-atmospheric storage could be a suitable method of increasing the shelf-life of fruits.

Using Response Surface Methodology to Optimize Edible Coating Formulations to Delay Ripening and Preserve Postharvest Quality of Tomatoes

Tomato is a nutrient-rich but highly perishable fruit. In order to delay the rapid ripening and degradation of fruits and reduce postharvest losses, response surface methodology (RSM) was used as the optimizing method to formulate edible coating based on pineapple peel extract and Arabic gum of twenty concentrations of pineapple (0.5–0.83 kg/l) and 20 concentrations of Arabic gum (5–15%, w/v). Tomatoes were soaked for 10–30 min in any of the coating solution. Five parameters including ripening rate, chlorophyll a content, firmness, total flavonoid content, and titratable acidity of tomatoes were evaluated after 8 days of storage at 24 ± 0.5°C and 82 ± 1.5% relative humidity. Results showed that the experimental data could be adequately fitted into a second-order polynomial model with coefficient of determination (R2) ranging from 0.775 to 0.976 for all the variables studied. The optimum concentrations were predicted as 0.70 kg/l pineapple peel extract and 17.04% with 18.72 min optimum time. Under these conditions, predicted values of response variables are as follows: ripening rate (RR) 40.75, chlorophyll a (Chl a) 8.11, firmness (Fir) 4.00, total flavonoid content (TFC) 43.51, and titratable acidity (TA) 0.30. It is concluded that RSM can be used to optimize pineapple peel extract and Arabic gum-based edible coating formulation to extend the shelf life or delay the ripening process of tomato fruit at ambient conditions.

Melatonin Treatments Reduce Chilling Injury and Delay Ripening, Leading to Maintenance of Quality in Cherimoya Fruit

Spain is the world's leading producer of cherimoya, a climacteric fruit highly appreciated by consumers. However, this fruit species is very sensitive to chilling injury (CI), which limits its storage. In the present experiments, the effects of melatonin applied as dipping treatment on cherimoya fruit CI, postharvest ripening and quality properties were evaluated during storage at 7 °C + 2 days at 20 °C. The results showed that melatonin treatments (0.01, 0.05, 0.1 mM) delayed CI, ion leakage, chlorophyll losses and the increases in total phenolic content and hydrophilic and lipophilic antioxidant activities in cherimoya peel for 2 weeks with respect to controls. In addition, the increases in total soluble solids and titratable acidity in flesh tissue were also delayed in melatonin-treated fruit, and there was also reduced firmness loss compared with the control, the highest effects being found for the 0.05 mM dose. This treatment led to maintenance of fruit quality traits and to increases in the storage time up to 21 days, 14 days more than the control fruit. Thus, melatonin treatment, especially at 0.05 mM concentration, could be a useful tool to decrease CI damage in cherimoya fruit, with additional effects on retarding postharvest ripening and senescence processes and on maintaining quality parameters. These effects were attributed to a delay in the climacteric ethylene production, which was delayed for 1, 2 and 3 weeks for 0.01, 0.1 and 0.05 mM doses, respectively. However, the effects of melatonin on gene expression and the activity of the enzymes involved in ethylene production deserves further research.

Effect of Chitosan nanoparticles on quality indices, metabolites, and vase life of Rosa hybrida cv. Black magic

BackgroundChitosan nanoparticles (CTS-NPs) protect the active ingredients from the environment for a specific period and reduces sweating, control weight loss, delay ripening, and increase vase life. So, a factorial experiment was carried out as a randomized complete design in three replications to investigate the efficiency of CTS-NPs in quality improvement and longevity extension of cut rose flowers.ResultsThe 15-day maximum vase life was attained by the rose placed in a preservative solution containing 10 mg L−1 CTS-NPs. CTS-NPs also reduced microbial growth as compared to controls. Total phenolics, total flavonoids, and amount of anthocyanin in treated petals were dramatically increased. CTS-NPs solutions especially at 10 and 15 mg L−1 concentrations, markedly reduced the H2O2 and malondialdehyde at the end of 15th day and maintained the membrane index. The protein and carbohydrate and petals anthocyanin content and enzymatic activities such as superoxide dismutase, polyphenol oxidase, peroxidase, catalase and ascorbate peroxidase increased in cut roses placed in 10 mg L−1 CTS-NPs vase solution which in turn caused to increase in vase life.ConclusionCTS-NPs especially at a level of 10 mg L−1 can assist plants to enhance light usage efficiency, as well as promote photosynthetic carbon fixation and the production of additional carbohydrate products for plant growth and development.Graphical

Changes of physico-chemical parameters in relation to storage time and temperature of mango fruit cv. Kent harvested in northern Côte d'Ivoire for export purposes

The aim of this study was to evaluate the physicochemical parameters of mangoes for export. The results revealed that mangoes lose firmness after three days of storage and the pulp turns yellow-orange. The sugar content increases progressively along storage time. However, phenylalanine ammonia-lyase (PAL) activity decreases with storage time confirming the decrease of phenol content. As for the activity of polyphenoloxidases (PPO) and peroxidases (POD), it increased during the storage of mangoes suggesting a degradation of phenolic compounds during storage. Furthermore, the study reported a loss of firmness inversely proportional to the time (7, 14, 21-days) and temperature (4, 10, 14 °C) of mango storage. The pulp of mangoes stored at low temperature started to show orange color after 14 days of storage at 10 °C and after 7 days of storage at 14 °C. The sugar content of mangoes stored at 14 °C was higher than that of mangoes stored at 4 and 10 °C. Similarly, phenol content, PAL, PPO and POD activities were lower at 4 and 10 °C and high when mangoes were stored at 14°C. Therefore, storage time and temperature influence harvested mangoes physico-chemical parameters of mangoes. High storage times and temperatures promote rapid ripening of mangoes by increasing sugar content and color, and decreasing pulp firmness. Furthermore, phenol synthesis decrease and oxidation increase was observed. Also, low temperatures delay ripening and preserve the quality of mangoes for up to 21 days. This makes it possible to envisage an export without deterioration of mangoes, which requires a maritime transport of 14 days.

Attenuating sugar spot and retaining quality of banana fruits by combined use of hot water and calcium lactate during storage.

Combined use of hot water (HW) treatment and calcium lactate (CL) is a promising postharvest approach to preserve the food value and prolong the shelf life of fruits. The present experiment aims to determine the physiological loss in weight, firmness, respiration rate, ethylene and biochemical attributes of banana fruits treated with hot water (50 °C for 7 min) and aqueous CL dipping (1, 2, and 3% for 2 min). Treated fruits were stored under ambient conditions (22-25°C temperature and 60-65% of relative humidity) for up to 9 days. The study showed that combined use of HW and CL (3%) maintained higher hue angle, peel firmness (4.4 N), reduced decay loss (10.63%), respiration and ethylene evolution rate of stored fruits. Also, CL treatments (3%) with HW proved the best which reduced 6-fold sugar spot and 1.5-fold decay loss over untreated fruits. At the end of storage sensory parameters such as mouthfeel, peel colour and overall acceptability (score 6.9) were recorded higher in HW and CL 3% treated fruits. The findings indicated that pre-storage combined use of HW and CL has a great potential to preserve quality, delay ripening, and reduce sugar spots, and postharvest decay loss in banana fruit without any adverse effect on consumer appeal.

Tragacanth gum coating suppresses the disassembly of cell wall polysaccharides and delays softening of harvested mango (Mangifera indica L.) fruit

Mango is a climacteric fruit and is prone to high perishability. The rapid softening and ripening (due to degradation and disassembly of cell wall polysaccharides) are the major limitations in extending the storability of the harvested mango fruits. Various types of gum-based edible coatings have been reported for the shelf life extension of mango fruits. Tragacanth gum (TCG) also has appropriate coating properties. Its use as an edible coating has been reported on certain fruits. However, the effect of TCG coating in the regulation of harvested mango fruits ripening and softening has not been reported yet. So, the objective of this work was to investigate the effect of TCG (control, 0.5 %, 1 % and 1.5 %) coating on postharvest softening and ripening of harvested mango fruits. TCG coating affected the ripening and softening of mango in a dose-dependent manner. Results exhibited that mango fruits coated with 1.5 % TCG showed substantially lower disease incidence and weight loss. The 1.5 % TCG-coated mangoes showed substantially lower ethylene biosynthesis and respiration rate peaks as well as superoxide anion and hydrogen peroxide contents compared with the control. In the same way, 1.5 % TCG-coated mango fruits had markedly higher total chlorophyll content and lower L*, b* and a* along with substantially lower total carotenoids in peel tissues. Mango fruits coated with 1.5 % TCG exhibited markedly lower water-soluble pectin and higher chelate-soluble pectin, Na2CO3-soluble pectin, protopectin, cellulose and hemicellulose in flesh tissues compared with control. The activity of polygalacturonase (PG), cellulase (CX), pectin methylesterase (PME), β-galactosidase (β-Gal) and β-glucosidase (β-Glu) were significantly lower in flesh of 1.5 % TCG treated fruits along with substantially higher firmness in contrast with control. In addition, 1.5 % TCG coating treatment showed significantly higher activity of antioxidative enzymes and delayed the increase in soluble solids content (SSC) and ripening index (RI) along with considerably higher titratable acidity (TA) compared with the untreated control. So, pre-storage TCG based edible coating (1.5 %) could be applied to delay ripening and softening in mango fruit industry under postharvest ambient conditions.

Recent Advancements of Polysaccharides to Enhance Quality and Delay Ripening of Fresh Produce: A Review.

The freshness of fruits and vegetables plays a significant role in consumers’ decision to purchase a product at the supermarket. Fresh-cut products are the latest trend in fulfilling society’s restless needs, and the food industry is faced with the challenge of maintaining the quality of fresh produce. The food industry is concerned with the natural maturation and degradation of fruits and vegetables, primarily due to enzymatic reactions. It has been demonstrated that polysaccharide coatings effectively preserve the freshness of these products, extending their shelf life depending on the preservation method used. This review informs readers about the different types of polysaccharides and their novel applications as natural food preservatives in the past five years (2018–2022). The key findings summarized the properties of the antimicrobial agent, the molecular mechanism of action, coating methods, and formulation for the preservation approach. Additionally, we discuss the scientific factors influencing polysaccharide processing and preservation efficacy, allowing it to be used in post-harvest management.

Impact of post-harvest application of chemicals and packaging materials on ripening and shelf-life of banana (Musa paradisiaca L.) cv. Grand Naine

An experiment was conducted in Department of Fruit Science, College of Agriculture, Indira Gandhi Krishi Vishwavidalaya Raipur (C.G.) during 2021–22 to delay ripening and extend shelf-life of banana (Musa paradisiaca L.) fruit. under the present investigation, the packaging materials were used individually as well as in combinations with different chemicals i.e. CaCl2 4%, CaCl2 6%, NaCl 0.2%, NaHCO3 0.3% and NaHCO3 0.4%. The treatments were viz. T0-Control (untreated), TrCaCl2 4%, T2-CaCl2 6%, T3-NaCl 0.2%, T4-NaHCO3 0.3%, T5-NaHCO3 0.4%, T6-Polyethylene bag LDPE 150 gauge, T7-Newspaper, T8-CaCl2 4% + Polyethylene bag LDPE 150 gauge, T9-CaCl2 6% + Polyethylene bag LDPE 150 gauge, T10-CaCl2 4% + Newspaper, T11-CaCl2 6% + Newspaper, T12-NaCl 0.2% + Polyethylene bag LDPE 150 gauge, T13-NaCl 0.2% + Newspaper, T14-NaHCO3 0.3% + Polyethylene bag LDPE 150 gauge, T15-NaHCO3 0.4% + Polyethylene bag LDPE 150 gauge, T16-NaHCO3 0.3% + Newspaper and T17-NaHCO3 0.4% + Newspaper. The treatment T9 (CaCl2 6% + Polyethylene bag LDPE 150 gauge) were the most potent treatment for enhancing storage period and maintaining physico-chemical characteristics of banana cv. Grand Naine while as CaCl2 6% + Polyethylene bag LDPE 150 gauge were very effectively reduced physiological loss in weight (PLW) and ripening per cent. The maximum retention of bio-chemical constitute like total soluble solids, titratable acidity, and reducing sugars was also obtained in fruits treated with CaCl2 6% + Polyethylene bag LDPE 150 gauge along with increased shelf-life up to 20th day storage period of banana fruits.

Does Shade Impact Coffee Yield, Tree Trunk, and Soil Moisture on Coffea canephora Plantations in Mondulkiri, Cambodia?

Shade is a natural condition for coffee plants; however, unshaded plantations currently predominate in Asia. The benefits of shading increase as the environment becomes less favorable for coffee cultivation, e.g., because of climate change. It is necessary to determine the effects of shade on the yield of Coffea canephora and on the soil water availability. Therefore, three coffee plantations (of 3, 6, and 9 ha) in the province of Mondulkiri, Cambodia, were selected to evaluate the effect of shade on Coffea canephora yields, coffee bush trunk changes, and soil moisture. Our study shows that shade-grown coffee delivers the same yields as coffee that is grown without shading in terms of coffee bean weight or size (comparing average values and bean variability), the total weight of coffee fruits per coffee shrub and the total weight of 100 fruits (fresh and dry). Additionally, fruit ripeness was not influenced by shade in terms of variability nor in terms of a possible delay in ripening. There was no difference in the coffee stem diameter changes between shaded and sunny sites, although the soil moisture was shown to be higher throughout the shaded sites.